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Volume 86 (1965)
- Issue 4 Pages 301-
- Issue 3 Pages 201-
- Issue 2 Pages 93-
- Issue 1 Pages 1-
Volume 86, Issue 4
Displaying 1-11 of 11 articles from this issue
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Tsuneo Arakawa, Kazuo Ohara, Rokuro Kakizaki, Yoshihiko Takahashi, Kei ...1965 Volume 86 Issue 4 Pages 301-306
Published: September 25, 1965
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSFolio acid deficiency was demonstrated in a child with hemochromatosis probably due to numerous blood transfusions for pure red cell anemia.
A defective storage of folio acid was suggested as an important factor con-tributing to the development of folio acid deficiency in hemochromatosis, basing upon the findings showing a rapid folio acid clearance from blood and the absence of defective intestinal absorption of folio acid of either free or conjugated form.View full abstractDownload PDF (306K) -
Zensaku Yosizawa, Eiaki Tsutsumi, Minro Watanabe1965 Volume 86 Issue 4 Pages 307-310
Published: September 25, 1965
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSIncorporation of Glucose-14C, in vitro, into the sugar components of the three carbohydrate fractions of colonic mucosa from liver tumor (3'-methyl-4-dimethyl-aminoazobenzene-induced liver tumor)-bearing rats was studied, comparing with that from normal younger rats.
The results showed that the rate of incorporation of glucose-14C into the sugar components of polysaccharide-containing fraction (PS) was small, but those of carbohydrate fraction eluted with 50% aqueous ethanol from the charcoal-absorbed substances (Et) and of carbohydrate fraction eluted with aqueous ethanol-ammonia from the charcoal-absorbed substances (Et-Am) were fairly large. And glucose-14C incorporated much more into these carbohydrate fractions from the tumor-bearing rats than those from normal animals.View full abstractDownload PDF (237K) -
Atsuo Suzuki1965 Volume 86 Issue 4 Pages 311-324
Published: September 25, 1965
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThis study was carried out in order to establish a simple and stable method to demonstrate acid phosphatase activity of leucocyte on blood smear. Settling a tentative method according to Pearse's azo dye method for tissue section, each experiment was carried out to choose the most suitable condition at each step of the procedure. It was made clear that the following method was a simple and reliable one. 1) Prepare blood or bone marrow smears. 2) Dry the smears. 3) Fix for 5 minutes in neutral formalin vapor at room temperature below 20°C with-in 60 minutes after the smear preparation. 4) Expose the smears to air for 1 hour to remove formalin. 5) Prepare the incubating solution as follows. Fifty mg of α-naphthylphosphate, 50mg of Fast-Garnet-GBC salt, 50ml of acetate buffer (pH 5.0, 0.1M) are mixed, shaken well, and filtrated. 6) Incubate in it for 90 minutes at 37°C. 7) Wash well in distilled water. 8) Counterstain nuclei with Meyer's hematoxylin for 30 minutes. 9) Wash for 5 minutes in tap-water. 10) Mount in 40% PVP and microscopy.View full abstractDownload PDF (577K) -
Masatoshi Sakka1965 Volume 86 Issue 4 Pages 325-333
Published: September 25, 1965
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSLife span of adult Sarcophaga peregrina became short after X-irradiation given in early pupal stage. It also inhibited imago formation of irradiated pupae. Shortening of life was adequately explained by upward shift of age specific mor-tality curve. In a range between zero and a single dose of about 2, 000 roentgen which inhibited 90 per cent of imago formation, there was a possible correla-tion of short-term and long-term effects and two kinds of injuries reduced when a dose was fractionated. Mitotic inhibition of disc cells induced by radiation plays some role in these effects.View full abstractDownload PDF (417K) -
Isao Miwa1965 Volume 86 Issue 4 Pages 334-340
Published: September 25, 1965
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSAn incubation method for the estimation of urinary kallikrein is described. It consists of incubation of urine with heated human plasma substrate (56_??_58°C, 3 hrs.) and assay of formed kinin on the guinea-pig ileum against synthetic brady-kinin as a standard. Kallikrein content of the urine is calculated by comparing its kinin forming activity with that of standard urine.
Twenty-four hour excretions of urinary kallikrein were 23_??_600 Frey units in total 58 individuals including both healthy and variously diseased subjects. These values are in good accordance with those of former authors who estimated kallikrein on dog's blood pressure. No significant difference was observed in kallikrein excretion between various diseases.View full abstractDownload PDF (329K) -
Ryo Chiba, Mitsuko Sato, Tsuneo Arakawa1965 Volume 86 Issue 4 Pages 341-347
Published: September 25, 1965
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSBy using 57CoB12 an intestinal absorption of vitamin B12 and vitamin B12-binding power of the gastric mucosa of rats with vitamin B2 deficiency were investigated. The results revealed that a decrease in vitamin B12 in the liver and serum of rats with vitamin B2 deficiency was a result from a defective intestinal absorption of vitamin B12, and that this malabsorption may be due to a decrease in ‘intrinsic factor’ caused by vitamin B2 deficiency.View full abstractDownload PDF (305K) -
Susumu Majima, Iwao Yamaguchi, Katsumi Karube, Teiichi Teshima, Koichi ...1965 Volume 86 Issue 4 Pages 348-365
Published: September 25, 1965
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe mechanism of carcinogenesis in the stomach is still unknown. However, accumulation of information on histopathology of early gastric carcinomas will provide a clue for elucidation of true pro-cancerous conditions and will eventually lead to solution to the problem. The authors studied 95 selected cases of early carcinoma that were encountered at their surgical depart-ment and tried to identify the conditions on which bases the malignancies had developed. As reported previously, 10 of the cases (10.5%) were judged to have arisen from pro-existing gastric polyps and other 25 (26.3%) from chronic penetrating ulcers. Excluding these cases, this paper deals with the results with the other 60 cases - those which are customarily referred to as gastritis-carcinomas. Chronic gastritis appeared to be the most probable matrix of carcinoma in a large majority (38 cases) but not all of these 60 cases. Fifteen of the cases were suggestive of carcinogenesis from shallow gastric ulcer or its scar, and in the remaining seven both chronic gastritis and shallow ulcer were considered to be responsible for development of malignancy. Thus, the ulcerative lesion of the stomach, shallow or penetrating, should be regarded as one of the most significant conditions as regards the possibility of malignant change.View full abstractDownload PDF (5260K) -
Hiroshi Demura, Soitsu Fukuchi, Hidenori Takahashi, Koji Goto1965 Volume 86 Issue 4 Pages 366-379
Published: September 25, 1965
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe reactivity of blood vessels to DL-noradrenahne and angiotensin II was examined using the perfusion technic of isolated hindlimbs of rats and rabbits. Rats with Goldblatt hypertension and animals treated with DOCA, agniotensin II and NaCl; with D-aldosterone 21-monoacetate, angiotensin II and NaCl for 4 weeks; and with angiotensin II were found to show an increased reactivity to vasoactive substances, an increase in the vascular wall potassium content and decrease in serum potassium concentration. Rats treated with aminonucleoside and with carbon tetrachloride had a decreased reactivity and a decreased potassium content of their arterial walls. Vascular reactivity was elevated in the hind-limbs of rabbits perfused with the solution containing a high concentration of potassium, while it was almost normal when the solution containing a low concentration of potassium was used. The changes in reactivity of the hindlimb vessels did not always parallel the blood pressure changes.
These results show that the changes in the intracellular potassium concentra-tion seem to be more important determinants of the changes in the reactivity of the hindlimb vessels to vasoactive agents than the changes in the extracellular potassium concentration.View full abstractDownload PDF (729K) -
Tatsunori Tsuda1965 Volume 86 Issue 4 Pages 380-393
Published: September 25, 1965
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSTwo cell lines were established in vitro from Syrian hamster embryos. The shift of biological characteristics, such as cell morphology, growth rate, the ability to grow at low inoculation density, chromosome constitution and tumor-producing capacity was examined for ten months. The growth rate upon successive transfers declined slowly and about three months after the initial cultures cellular growth stopped. Thereafter colonial growth was performed and the growth rate began to rise which soon reached a value higher than that at the culture onset. No distinctive morphological alteration was observed after the colony formation. Chromosomal studies indicated that the cells before the phase of declining growth rate were diploid, but after colony formation, the population shifted to the hyperdiploid range. The cells implanted periodically into isologous hamsters never gave tumors.View full abstractDownload PDF (1154K) -
Tatsuo Koyanagi, Hitoshi Obori1965 Volume 86 Issue 4 Pages 394-396
Published: September 25, 1965
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSAmount of taurine has increased in urine of rats fed on pantothenicc acid-deficient ration. In contrast, it has decreased in pyridoxine-deficient rats. The increase in pantothenic acid-deficient rats might be resulted from hyperfunction of adrenals and the decrease in vitamin B6-deficient rats would be due to lowered activity of evsteinsulfinic acid decarboxvlase in liver.View full abstractDownload PDF (165K) -
Yoichiro Sasai1965 Volume 86 Issue 4 Pages 397-404
Published: September 25, 1965
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe application of the graphical method of Lineweaver and Burk to the histochemical system was attempted to obtain qualitative information as to the nature of phosphorylase activity. As substrate for the reciprocal of the reaction velocity, the time required for the first appearance of phosphorylase reaction in a tissue site at various substrate concentrations were plotted against the reciprocal of the substrate concentrations. This technic made it possible to examine precisely the effects of adenylic acid, adenosine triphosphate and Mg++ as the activators, and sodium fluoride, ethylendiaminetetraacetic acid as inhibitory agents. On the basis of these data, it was suggested that the decrease in phosphorylase a following cantharidin application was caused by the alteration of the respective activities of phosphorylase kinase, phosphorylase phosphatase and proteolytic enzymes.View full abstractDownload PDF (416K)
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