UZUKA, Y. and SAITO, Y. THP-ADM in the Treatment of AcutePromyelocytic Leukemia. Tohoku J. exp. Med., 1985, 146 (1), 1-8-Three patients, diagnosed as acute promyelocytic leukemia and disseminated intravascular coagulation (DIC), were treated with THP-ADM in combination with heparin and intensive platelet transfusion. Two of the three patients achieved complete remission. The remaining one patient also responded favorably to the therapy and achieved marrow aplasia, and significant improvement of coagulopathy was observed after chemotherapy. However, he suddenly died of intractable congestive cardiac disturbance ten days after the completion of THP-ADM induction therapy. The mechanism of this unique delayed anthracycline-associated cardiotoxicity was discussed.
SATO, E., GOTO, M. and SAKU, T. Nuclear Changes in Colo-Rectal Epitheliumwith Special Reference to Nuclear Inclusions in Carcinoma, Dysplasia, Adenomaand Peutz-Jeghers Polyps. Tohoku J. exp. Med., 1985, 146 (1), 9-16-The incidence and types of unusual nuclear structures (UNS) were examined based on the observation of randomly photographed 1, 919 nuclei in total from the normal epithelium, dysplasia, Peutz-Jeghers type polyp, adenoma and carcinoma of the large bowel. These nuclear structures were classified as filamentous, granular and vesicular inclusions, and invaginated structures were also taken into consideration. Although all types of UNS were found in the normal epithelium, carcinoma cells had the most numerous UNS, especially of the invaginated type. The possible process of nuclear inclusion (NI) formation was discussed in connection with malignant transformation of colo-rectal epithelial cells.
CHIBA, T., MOCHIZUKI, I., HANAMATSU, M., OHI, R. and KASAI, M. Studies onthe Changes of Serum Bilirubin Level after Surgery in Biliary Atresia. Tohoku J. exp. Med., 1985, 146 (1), 17-25-In biliary atresia, one-fourth of the patients showed prolonged jaundice in spite of a good excretion of bile after surgery. To investigate the cause of jaundice, factors related to the serum bilirubin level were examined in 28 recent cases. Furthermore, clinical analysis was performed in 86 cases of good excretion of bile after surgery. The following results were obtained: 1) The daily amount of bilirubin excretion in bile and bilirubin concentration play an important role in decreasing the serum bilirubin level. 2) The most important factor for prolonged jaundice is ascending cholangitis. 3) Shortening of the life span of the red blood cells is one of the causes of prolonged jaundice.
TOKI, T., OIKAWA, N., TASE, T., WADA, Y., YAJIMA, A. and SUZUKI, M. Immunohistochemical Localization of Carcinoembryonic Antigen in Adenocarcinomaof the Endocervix and the Endometrium. Tohoku J. exp. Med., 1985, 146 (1), 27-32-Immunoperoxidase localization of carcinoembryonic antigen (CEA) was performed on tissue sections of adenocarcinoma of endocervical and endometrial origin to clarify the criteria for their differentiation. Twenty-two (96%) of 23 endocervical and 32 (70%) of 46 endometrial adenocarcinomas revealed positive CEA immunostain. The extent of staining did not seem to correlate with the degree of differentiation in either kind of cancer. Among the positive cases, CEA staining was located over whole cytoplasm in 18 (80%) of 22 positive endocervical adenocarcinomas and on apical surface in 24 (75%) of 32 positive endometrial adenocarcinomas. The pattern of CEA distribution in endocervical adenocarcinoma of endometrioid type was not similar to that observed for primary endometrial adenocarcinoma.
YASUOKA, S., NAKAYAMA, T., KAWANO, T., OGUSHI, F., DOI, H., HAYASHI, H. and TSUBURA, E. Comparison of Cell Profiles of Bronchial and BronchoalveolarLavage Fluids between Normal Subjects and Patients with Idiopathic PulmonaryFibrosis. Tohoku J. exp. Med., 1985, 146 (1), 33-45-The cell profiles of bronchial and bronchoalveolar lavage fluids (BLF and BALF) of patients with idiopathic pulmonary fibrosis (IPF) were compared with those of normal volunteers (NV) and age-matched control patients (CP), to characterize the cell profiles of the bronchoalveolar region in normals and patients with IPF. In BALF of nonsmokers from both control groups (NV and CP), alveolar macrophages (AM) were predominant and the percentage of neutrophil leukocytes and that of eosinophil leukocytes below 1% of the total cells. The percentage of neutrophils and that of bronchial epithelial cells were higher in BLF than in BALF of both control groups. Of the immune and inflammatory cells in BLF, the mean percentage of neutrophils was 12% in NV group and 42% in CP group. The percentage of neutrophils and that of eosinophils in BALF were higher in IPF group than in CP group, but the percentage of neutrophils in BLF of IPF group was comparable to that of CP group. In the IPF group, the percentage of neutrophils in BALF was lower than that in BLF. These results indicated that even in healthy subjects, a considerable number of neutrophils are present in the bronchial region and that the cell profile of the lavage fluid of the bronchoalveolar tree changes depending on the method of lavage. Presumably the higher percentage of neutrophils in BALF of patients with IPF is partly due to derangements of the alveolar structure, because the amount of saline infused into this region is limited
OHNEDA, A., KOBAYASHI, T., NIHEI, J. and SAKAI, T. Secretion of Glucagon inLiver Cell Carcinoma. Tohoku J. exp. Med., 1985, 146 (1), 47-57-In order to elucidate the response of plasma glucagon in liver cell carcina, a clinical study was performed in 12 patients with liver cell carcinoma in addition to 8 patients with liver cirrhosis and 8 normal subjects. Arginine infusion elicited increases in plasma insulin and glucagon in 6 patients with liver cell carcinoma as well as 8 patients with liver cirrhosis compared with the controls. However, the responses of plasma insulin and glucagon in liver cell carcinoma did not exceed those in liver cirrhosis. No glucagon secreting cell was proved in the hepatic cancer tissues from two other patients. Furthermore, no measurable glucagon was demonstrated in the tumor tissues extracted from four other patients with liver cell carcinoma. The extract of the tumors, infused into the pancreatic artery of anesthetized dogs, did not elicit any discernible changes in glucagon and insulin in the pancreatic vein. The present study demonstrates an elevated response of plasma glucagon in liver cell carcinoma. Since the morphological and biochemical studies failed to demonstrate the glucagon secreting cell or glucagon-stimulating material in the tumor tissues, the elevated plasma glucagon response might be interpreted by the increased A-cell function of the pancreas and the decreased degradation of the hormones in the liver.
ABE, T., SUZUKI, M., WADA, Y., YAMAYA, Y. and MORITSUKA, T. Clinicaland Endocrinological Features of Statistical Clusters of Women with ClimactericSymptoms. Tohoku J. exp. Med., 1985, 146 (1) 59-68-Clinical and endocrinological features of women with climacteric symptoms, who were clustered by means of factor analysis and cluster analysis, were evaluated as to serum concentrations of estradiol, progesterone, follicle-stimulating hormone and luteinizing hormone, and also as to mean ages as well as a rate of premenopausal women or that of postmenopausal women against all women in each one of the clusters. Comparison of these parameters among these clusters indicated, with statistical significance, that vasomotor symptoms, neurotic symptoms, and depressive state of the climacteric women are endocrinologically characterized by a depressed level of serum estrogen, relatively high level of serum estrogen, and an elevation of serum gonadotropin, particularly of luteinizing hormone, respectively.
ISHIKAWA, Y., MINAMI, R. and NAKAO, T. Diagnosis of AdrenoleukodystrophyUsing Dried Blood Spots. Tohoku J. exp. Med., 1985, 146 (1), 69-72-We have demonstrated the increased C26:0/C22:0 ratio in the fatty acids of sphingolipid fraction in dried blood spots on filter paper from the patient with adrenoleukodystrophy. The ratio of C26:0/C22:0 in the dried blood spots from the patient was 2.1-fold higher than those of the normal controls. This value was almost the same as the ratio in erythrocyte membrane sphingomyelin from the patient, because sphingolipid fraction in the dried blood spots consists of mainly the sphingomyelin from erythrocyte membranes. Blood samples are easy to obtain by mail and the method is useful in the diagnosis of the patient and screening for the families known to be at risk.
YOSHIKAWA, K., YAMAGUCHI, H., OJIMA, M., MASUDA, T. and SASANO, N Particular Cases of Cushing's Syndrome-One with Adrenocortical Carcinomaand the Other with Double Adenomas-. Tohoku J. exp. Med., 1985, 146 (1), 73-82-Two particular cases of adrenocortical tumors associated with Cushing's syndrome were documented. In the first case, the steroid pattern suggested a malignancy, and the histology of the excised tumor confirmed it. In the second case, the left adrenal had two distinct tumors; one dark-brown, and the other yellow in color. Recent trends in the diagnosis and treatment of Cushing's syndrome were discussed.
MAJIMA, M., NAKAMURA, T. and ENDO, M. Urinary GlycosaminoglycansBearing Glucuronic Acid or Iduronic Acid Residue at the Reducing Terminals. Tohoku J. exp. Med., 1985, 146 (1), 83-88-The urinary glycosaminoglycan was reduced with NaB [3H]4, and was separated into three fractions according to molecular size by column chromatography on Sephadex G-200. The hexuronic acid residues at the reducing terminals of each fraction were quantified by the method of preferential quantitation of hexuronic acid at the reducing terminals. This method consisted of hydrolysis with trifluoroacetic acid and nitrous acid, lactonization, and paper chromatographic analysis. Both radioactive gulonolactone and idonolactone were detected in the fractions with a lower molecular size, but not in those with a higher molecular size. These observations demonstrated that the glucuronide and iduronide linkages at other-than-terminal sites of carbohydrate chains were cleaved by the processes of depolymerization of glycosaminoglycans in tissues. Therefore, it is highly likely that endo-β-glucuronidase(s) and endo-α-iduronidase(s) which belong to the glycosidase of endo type such as hyaluronidase are involved in the catabolic degradation of glycosaminoglycans in tissues.
KUMANO, N., SUZUKI, S., ISHIKAWA, T., KOINUMARU, S. and KONNO, K. Antitumor Effect of the Yeast Polysaccharide Preparation in Syngeneic MouseTumor Models. Tohoku J. exp. Med., 1985, 146 (1), 89-96-Antitumor effect of our Candida utilis glucomannan preparation (YPS) was evaluated in two syngeneic transplantable tumors; the Lewis lung carcinoma (3LL) in C57BL/6 (B6) mice and the 3-methylcholanthrene (MCA)-induced fibrosarcoma in C3H/He mice. YPS inhibited preferentially the 3LL pulmonary metastases only when optimal dosage was given at an earlier stage; 7 daily intraperitoneal (i.p.) doses of 100mg/kg per dose from the next day of tumor inoculation (1×105/mouse, subcutaneously, s.c.) substantially reduced the number of metastatic nodules, yielding the inhibition ratio 54% on day 21. However, the same treatment delayed until day 8 markedly facilitated the metastatic spread (-105%). Postsurgical-adjuvant therapy with YPS (10 daily i.p. doses of 100mg/kg per dose from the next day of surgery) was effective in improving the survival rate with no evidence of metastatic growth on day 200, even though the amputation of the tumor-bearing legs was performed after the pulmonary metastases had been established (on day 20). In the MCA-fibrosarcoma model, YPS treatment from day 3 (10 daily doses of 100mg/kg per dose) was effective in prolonging the survival period. The 3LL local tumor was affected only marginally and temporarily, while the MCA-tumor was not at all.
KUMANO, N., ISHIKAWA, T., KOINUMARU, S., KIKUMOTO, T., SUZUKI, S., NAKAI, Y. and KONNO, K. Antitumor Effect of the Organogermanium CompoundGe-132 on the Lewis Lung Carcinoma (3LL) in C57BL/6 (B6) Mice. Tohoku J. exp. Med., 1985, 146 (1), 97-104 -Effects of the organogermanium compound Ge-132 (i.p.) were examined on the 3LL local tumor (1×105/mouse, s.c.) and its pulmonary metastases in B6 mice. A characteristic feature of its action was the preferential antimetastatic effect under strictly defined conditions. Either inhibition or facilitation was observed depending on the treatment schedules; 7 daily doses of 100mg/kg yielded the inhibition ratio 49% when started from day 1, whereas the treatment from day 8 resulted in the ratio -99%. The maximum inhibition was obtained at 100mg/kg. The postsurgical-adjuvant treatment with Ge-132 was of no beneficial effect. The local tumor growth was affected only marginally and temporarily. When inoculum size was minimized (1×104), a single dose of 300mg/kg on day 1, but not on day 8, was effective in prolonging the latency before tumor take. The antitumor action of Ge-132 was discussed with reference to its interferon (IFN)-inducing activity.
SUZUKI, H., JUWONO, S.S. and MATSUMOTO, K. Invasion of Haemophilusinfluenzae and Pseudomonas aeruginosa in Humans. Tohoku J. exp. Med., 1985, 146 (1), 105-117-Levels of serum IgG antibodies to somatic antigens of Haemophilus influenzae and Pseudomonas aeruginosa were determined in 127 healthy people, 37 patients with chronic bronchitis and 20 patients with bronchiectasis by enzyme-linked immunosorbent assay. The logarithmic mean titers of antibodies to Haemophilus influenzae and Pseudomonas aeruginosa were significantly higher in the patients with chronic bronchitis and bronchiectasis than in healthy people. The invasion rate of Haemophilus influenzae was identical in healthy people, chronic bronchitis and bronchiectasis, while that of Pseudomonasaeruginosa was significantly lower in healthy people than in chronic bronchitis and bronchiectasis patients. These results indicate that patients with chronic bronchitis and bronchiectasis may often have recurrence and are easily infected because of lowered defense mechanisms in the respiratory tract.
TSUJI, I., NAGASAKI, H. and NAKAMURA, R. Motor Time of the RectusFemoris under Loading of Different Weights. Tohoku J. exp. Med., 1985, 146 (1), 119-120 -Motor time (MT) of the rectus femoris was measured for seven normal subjects during rapid extension of the knee joint at 60° flexion in the sitting position with and without external loads on the leg. MT prolonged linearly with external load from 0 to 4kg. Tangent of this linearity, the rate of tension development (RTD), was significantly different from one another of the subjects and was not related to MT without external load. RTD is a relevant index to assess an efficiency of rapid force generation of a muscle.
TAKISHIMA, T., YAMAUCHI, K., ISHII, M., CHAO, C.-L., IIJIMA, H., SHIMURA, S., SHINDOH, Y., INOUE, H. and MUE, S. Late Pulmonary Response in Guinea Pigsafter Ascaris Challenge. Tohoku J. exp. Med., 1985, 146 (1), 121-122-Respiratory resistance of guinea pigs immunized with Ascaris suum was measured over 6hr after aerosol challenge of the allergen by use of the oscillation method. These animals showed a biphasic pulmonary response to the aerosol challenge with Ascaris suum. Histological analysis of these animals revealed a narrowing of bronchial lumen, peribronchial edema and neutrophil-rich mucous in bronchial lumen at the late pulmonary response.