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Volume 161 (1990)
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Volume 161, Issue 1
Displaying 1-10 of 10 articles from this issue
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KAMRUDDIN AHMED, KEIZO MATSUMOTO, NAOTO RIKITOMI, TSUYOSHI NAGATAKE, T ...1990 Volume 161 Issue 1 Pages 1-7
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSAHMED, K., MATSUMOTO, K., RIKITOMI, N., NAGATAKE, T., YOSHIDA, T. and WATANABE, K. Effect of Ampicillin, Cefmetazole and Minocycline on the Adherence of Branhamella catarrhalis to Pharyngeal Epithelial Cells. Tohoku J. Exp. Med., 1990, 161(1), 1-7 - Using pharyngeal epithelial cells from a healthy adult and eight strains of Branhamella catarrhalis (B. catarrhalis) isolated from eight patients with respiratory infection the effect of subminimal inhibitory concentrations of cefmetazole, ampicillin and minocycline on adherence was examined. Cefmetazole-treated bacterial attachment (44±28; mean±S.D.) decreased significantly (p<0.05) compared to the control (84±27). Statistically no significant difference in adherence was found between ampicillin-treated bacteria (63±36) and the control (95±40) or minocycline-treated bacteria (91±39) and the control (109±40). Large bacteria was observed after cefmetazole and ampicillin treatment. In addition to diplococci, tetrads were observed after cefmetazole treatment. Significant correlation between the MICs and adherence ability was not found. The results suggests that these three antibiotics were not responsible for the increase in B. catarrhalis infection by increasing adherence ability.View full abstractDownload PDF (1897K) -
TSUYOSHI YOKOI, TOSHIO KIMURA, KAORU SAGISAKA1990 Volume 161 Issue 1 Pages 9-18
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSYOKOI, T., KIMURA, T. and SAGISAKA, K. Individual Identification of Human Bloodstains Investigated with Hypervariable DNA Loci. Tohoku J. Exp. Med., 1990, 161(1), 9-18 - Seven kinds of hypervariable DNA probes, which recognize hypervariable loci, were applied for individual identification of human bloodstains. Allelic frequencies and their confidence intervals among unrelated Japanese individuals were previously reported from our laboratory, and codominant segregation of the polymorphism was confirmed in family studies. One-month-old bloodstains from ten individuals were investigated in the present study. The probability of matching was calculated using the database among Japanese population. Cumulative probability of matching from 7 kinds of hypervariable DNA probes was 1.1×10-11, which was 4.5×109 times higher than that from 6 kinds of common blood group markers such as ABO, MN, Rh-Hr, P1, Hp and PGM1. Accordingly, DNA polymorphism is considered to be informative enough for individual identification of bloodstains.View full abstractDownload PDF (1275K) -
KOROKU OTOKIDA, KAZUTERU OHIRA, MOTOKO ISHIKAWA, NAOSHI ARAKAWA, AKIHI ...1990 Volume 161 Issue 1 Pages 19-24
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOTOKIDA, K., OHIRA, K., ISHIKAWA, M., ARAKAWA, N., YOSHIDA, A., KAMIMURA, A., KOU, E. and KATO, M. A Case of Turner Syndrome with the Karyotype of 45, X/46, X, i(Xq) Associated with Acute Monocytic Leukemia. Tohoku J. Exp. Med., 1990, 161(1), 19-24 - An infertile 37-year-old woman was diagnosed as having acute monocytic leukemia (AMoL) (FAB classification; M5b). In addition, a diagnosis of infertile Turner syndrome was made, based on the presence of the ovarian dysplasia, abnormal physical features (short stature, lack of pubic hair, shield-like chest, etc.), and low urinary estrogen excretion with high plasma gonadotropin level. Karyotypes in the peripheral blood and bone marrow cells were mosaic 45, X and 46, X, i(Xq): isochromosome Xq, which were consistent with infertile Turner syndrome. No further chromosomal abnormalities were found during the course of her treatment for leukemia. This is the first report of the combination of Turner syndrome and AMoL. However, this patient did not have any of the other autosomal chromosomal abnormalities which are common in acute non-lymphocytic leukemias.View full abstractDownload PDF (574K) -
Sweat Protein Components Tested by SDS-Polyacrylamide Gel Electrophoresis Followed by ImmunoblottingNORI NAKAYASHIKI1990 Volume 161 Issue 1 Pages 25-31
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNAKAYASHIKI, N. Sweat Protein Components Tested by SDS-Polyacrylamide Gel Electrophoresis Followed by Immunoblotting. Tohoku J. Exp. Med., 1990, 161 (1), 25-31 - Sweat samples were collected in a sauna from 74 healthy volunteers (72 men and 2 women) and concentrated. SDS-polyacrylamide gel electrophoresis (SDS-PAGE) of the individual samples revealed, in general, five main proteins and four PAS positive components. In pooled sweat, a method of SDS-PAGE followed by immunoblotting with specific antisera or antibodies against 24 human serum components was applied, and three out of the five main proteins showed the same molecular weights and antigenicities corresponding to serum albumin (67, 000 Da), Zn-α2-glycoprotein (42, 000 Da) and lysozyme (14, 000 Da). Moreover, orosomucoid, transferrin, IgG and IgA were demonstrated in the pooled sweat. Although α1-antitrypsin was probably in the pooled sweat, other serum components could not be detected. On the pooled and individual sweat samples, anti-carcinoembryonic antigen (CEA) formed three bands at 42, 000, 19, 000 and 18, 000 Da, but the antibody did not react with normal serum. It might be considered from these molecular weights that those sweat components are CEA-related antigens.View full abstractDownload PDF (1117K) -
YUKARI KONISHI, RYUNOSUKE KANAMARU, CHIKASHI ISHIOKA, AKIRA ISHIKAWA, ...1990 Volume 161 Issue 1 Pages 33-42
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKONISHI, Y., KANAMARU, R., ISHIOKA, C., ISHIKAWA, A., SHIBATA, H., WAKUI, A. and DEI, T. The Changes in the Levels of Dihydrofolate Reductase mRNA and Its Gene Dosage in 5-Fluorouracil-Resistant L1210 Cells. Tohoku J. Exp. Med., 1990, 161(1), 33-42 - 5-Fluorouracil (5-FU)-resistant L1210 cell line (L1210/5-FU-1) was established in this laboratory, and maintained by serial passage in the peritoneal cavities of BDF1 mice. This and another 5-FU-resistant cell line (L1210/5-FU-2) showed approximately 50-fold increase in resistance to 5-FU, i.e., IC50 of 5-FU determined for wild type L1210 cells was 3×10-7M, whereas those for 5-FU-resistant lines, L1210/5-FU-1 and L1210/5-FU-2 were 1.65×10-5M and 1.35×10-5M, respectively. The incorporation of 3H-5-FU into L1210/5-FU-1 cells was about 57% of that observed in wild type L1210 cells. Northern blot analysis of DHFR mRNA obtained from 5-FU-sensitive and -resistant cell lines revealed four distinct bands of 1.6kb, 1.2kb, 1.0kb and 0.75kb in length. Although all these bands showed higher density in autoradiography in 5-FU-resistant lines than in wild type, no extra band was observed. Southern blot analysis of DHFR DNA, digested with the restriction enzymes, EcoRI, BamHI or HindIII, revealed no rearrangement. However, all the fragments were expanded, showing that DHFR gene increased in 5-FU-resistant cells. The karyotype analysis carried out for L1210/5-FU-1 showed abnormal banding region in a part of chromosome X, and this chromosomal aberration was considered to be the reflection of the amplification of DHFR gene. Many investigators have reported that thymidylate synthetase (TS), a target enzyme for 5-FU, increased in 5-FU-resistant cells and that the increase of TS was responsible for the drug resistance to 5-FU. The increase both in DHFR mRNA and DHFR DNA suggested the increase in DHFR and also in N5, N10 methylenetetrahydrofolate (methylene THF), a coenzyme of TS. The increase of methylene THF, together with the increase of TS, might result in the resistance of the cells to 5-FU.View full abstractDownload PDF (2799K) -
HIDEAKI KONG, YOUNG CHIN LIN, MAMORU YAMAGUCHI, ATSUSHI SANBUISSHO, FR ...1990 Volume 161 Issue 1 Pages 43-47
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKONG, H., LIN, Y.C., YAMAGUCHI, M., SANBUISSHO, A., ZUSPAN, F.P., FURUHASHI, N. and YAJIMA, A. The Protein Synthesis of Human Full Term Placenta Cell in Monolayer Culture System. Tohoku J. Exp. Med., 1990, 161(1), 43-47 - Protein synthesis of human placenta from cesarean section was analyzed by SDS gel electrophoresis using full term cell culture system. The qualitative pattern of cytoskeletal proteins before and after culture was also examined. After trypsinization, cytotrophoblasts were cultured for 20 days in the humidified incubator of 5% CO2 in 95% air. The confluency was obtained in 10 days after inoculation. The pattern of SDS-PAGE showed several protein bands including actin (43, 000 Da) and desmin (55, 000 Da) as major constituents of 12 and 20 day cultures. The significant differences between band appearances in samples before and after culturing were noted. The present results indicated that myosin may not be synthesized in high content, differing from previous observations. Cytoskeletal protein production seemed to be markedly enhanced in the cultured systemView full abstractDownload PDF (637K) -
TADASHI MISAWA, JUN ARAMAKI, YOSHIHARU CHIJIIWA, TEPPEI KABEMURA, TOSH ...1990 Volume 161 Issue 1 Pages 49-54
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSMISAWA, T., ARAMAKI, J., CHIJIIWA, Y., KABEMURA, T., NASU, T. and NAWATA, H. Vasoactive Intestinal Polypeptide Response to Ethanol in Dogs. Tohoku J. Exp. Med., 1990, 161(1), 49-54 - Only one report has described the ethanol-induced release of vasoactive intestinal polypeptide (VIP), and its mechanism of action is unknown. We studied changes in mesenteric immunoreactive VIP (IR-VIP) concentrations following the intrajejunal administration of 100ml of normal saline, 5% and 10% ethanol, and hypertonic saline which was isoosmolar to 10% ethanol (1, 670mOsm/liter) in dogs. Administration of 5% and 10% ethanol resulted in significant and dose-dependent increases in mesenteric IR-VIP. Mesenteric IR-VIP changes and incremental integrated responses to 10% ethanol and to hypertonic saline were the same. We concluded that ethanol-induced VIP release in dogs is mainly due to ethanol's hyperosmolarity.View full abstractDownload PDF (498K) -
MASANORI MUNAKATA, YUTAKA IMAI, NAOYOSHI MINAMI, SYUUICHI SASAKI, TOSH ...1990 Volume 161 Issue 1 Pages 55-64
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSMUNAKATA, M., IMAI, Y., MINAMI, N., SASAKI, S., ICHIJYO, T., YOSHIZAWA, M., SEKINO, H., ABE, K. and YOSHINAGA, K. Cosinor Analysis of Changes in Circadian Blood Pressure Rhythm with Aging in Spontaneously Hypertensive Rats. Tohoku J. Exp. Med., 1990, 161(1), 55-64 - Evolution of circadian rhythm of blood pressure with age in male spontaneously hypertensive rats (SHR) was compared with that in male Wistar Kyoto rats (WKY). Three different age groups (5-6 week old, 19-22 week old, 29-31 week old) were provided for each strain. Chronogram and cosinor method were used for time series analyses. In all age groups of WKY, a diurnal fall and a nocturnal rise in blood pressure (BP) as well as in heart rate (HR) were observed. There was no significant difference in acrophase between BP and HR in each age group of WKY. Regarding SHR, however, each age group demonstrated a different relation between circadian BP rhythm and HR rhythm. In 5-6 week old SHR, a difference in acrophase between BP and HR (about 6.9hr, p<0.1) was observed, which became more conspicuous with the increase in age, eventually presenting an inverted relation between BP and HR in 19-22 week old SHR. The relation between circadian BP rhythm and circadian HR rhythm in 29-31 week old SHR was almost identical with that in 19-22 week old SHR. In other words, a phase lag of acrophase of BP from that of HR already observed in young SHR increased with aging. The results indicate that parasympathetic periodicity remained unchanged even in SHR since circadian HR rhythm was similar in all groups, while the periodicity of sympathetic neural tone relating to the regulation of circadian BP rhythm seems to be disturbed. The mechanism responsible for development and maintenance of high BP in SHR may be linked to a disturbance in the sympathetic mechanism which regulates BP periodicity.View full abstractDownload PDF (1029K) -
SATOSHI SUMI, MIKIHISA OBAYASHI, MAYUMI MURAKAMI, JIN ITO, NOBORU OKAD ...1990 Volume 161 Issue 1 Pages 65-67
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSUMI, S., OBAYASHI, M., MURAKAMI, M., ITO, J., OKADA, N. and SHIBATA, Y. Acute Megakaryoblastic Leukemia in Down Syndrome, Following Thrombocytopenia with Antiplatelet Antibody. Tohoku J. Exp. Med., 1990, 161(1), 65-67 - We report a case of acute megakaryoblastic leukemia (AMKL) with antiplatelet antibody in a boy with Down syndrome. When the patient was admitted, his platelet count was 1.3×104/mm3 and antiplatelet antibody in the plasma was detected. Two months after admission, blasts, which showed positive reaction to both antiplatelet monoclonal antibody and platelet peroxidase, increased.View full abstractDownload PDF (1113K) -
MASAHARU YAMAMOTO, MASATO HAGA, SHUKO TAKAGI, KAZUO ENDOH, SEIKI ITO, ...1990 Volume 161 Issue 1 Pages 69-71
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSYAMAMOTO, M., HAGA, M., TAKAGI, S., ENDOH, K., ITO, S., YOSHIDA, K., KATO, K. and AKAM, S. HLA Antigens in Cancer of the Gallbladder. Tohoku J. Exp. Med., 1990, 161(1), 67-71 - Thirty one patients with gallbladder cancer (GBC) and 32 healthy controls were typed using antisera against 12 HLA-A, 31 HLA-B, 7 HLA-C and 13 HLA-DR antigens. The DR4 frequency of 61.3% (19/31) in GBC was significantly different from that of 28.1% (9/32) in the control (p=0. 00794 by Fisher's exact test). The relative risk and the etiologic fraction were 4.0 and 0.46, respectively.View full abstractDownload PDF (254K)
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