All issues
Volume 150 (1986)
- Issue 4 Pages 365-
- Issue 3 Pages 231-
- Issue 2 Pages 103-
- Issue 1 Pages 1-
Volume 150, Issue 4
Displaying 1-15 of 15 articles from this issue
- |<
- <
- 1
- >
- >|
-
MAKOTO WAKUI, YASUE FUKUSHI1986 Volume 150 Issue 4 Pages 365-371
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSWAKUI, M. and FUKUSHI, Y. Evidence for Suppression of Potassium Conductance by Noradrenaline in Smooth Muscle of Guinea-Pig Vas Deferens. Tohoku J. exp. Med., 1986, 150 (4), 365-371-The ionic dependency was examined in the reversal potential of the NA-induced membrane depolarization of smooth muscle of the guinea-pig vas deferens, using the double sucrose-gap method. In the control (5.9mM K) solution and solutions containing 20 and 60mM K ions, replaced with equimolar amount of Na ions, the reversal potential was negative to the membrane potential at the steady state in each solution, by 19.5, 15.1 and 13.2 mV, respectively. Taking account that the higher external K concentration causes the larger membrane depolarization, these results indicate that the reversal potential shifted toward the positive direction as the external K concentration was increased. On the other hand, the calculated equilibrium potential of K ions in those solutions is -88, -52 and -24mV, respectively. In those solutions, furthermore, the equilibrium potential of Na ions is 42, 38 and 29mV, respectively. However, the equilibrium potentials of Ca and Cl ions should be unchanged. Therefore, it is suggested that the reversal potential of the NA-induced depolarization varied in the given different solutions compatibly only with the change in the K equilibrium potential. This provides evidence that in this tissue, NA induces a reduction in the K conductance of the cell membrane.View full abstractDownload PDF (766K) -
KENJI OKABE, PAUL S. MALCHESKY, YUKIHIKO NOSE1986 Volume 150 Issue 4 Pages 373-379
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOKABE, K., MALCHESKY, P.S. and NOSE, Y. Protective Effect of Prostaglandin I2 on Hepatic-Mitochondrial Function of the Preserved Rat Liver. Tohoku J. exp Med., 1986, 150 (4), 373-379-Mitochondrial function of the liver is one of the limiting factors in liver preservation. Nowadays, prostaglandin I2 (PGI2) is used as a cytoprotective agent in liver preservation without full understanding its mechanism involved. It is the objective of the present study to evaluate the protective effect of PGI2 on mitochondrial function of the rat liver during hypothermic preservation. Collins' solution was used as a preservation solution and PGI2 was added to it in some experimental groups. Both ischemic and nonischemic livers were perfused with the preservation solution and preserved under simple hypothermia for 8hr. Parameters of the mitochondrial function such as respiratory control, oxygen consumption rate in state 3 respiration, ADP/O ratio and the rate of ATP synthesis were decreased significantly after 8hr hypothermic preservation, even if ischemic injury was not induced prior to preservation. ADP/O ratio, which represents the efficiency of oxidative phosphorylation in mitochondria, was improved significantly (p<0.01) when PGI2 was used as a cytoprotective agent. The rate of ATP synthesis, a parameter of energy producing reaction, showed a tendency to be increased by PGI2, but was not significant. It was concluded that hypothermic preservation of the rat liver is associated with the deterioration of the mitochondrial function and PGI2 has a favorable effect on the impaired ADP/O ratio of oxidative phosphorylation in mitochondria.View full abstractDownload PDF (754K) -
MAKOTO WAKUI, YASUE FUKUSHI1986 Volume 150 Issue 4 Pages 381-389
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSWAKUI, M. and FUKUSHI, Y. Possible Relationship between the Release of Calcium Ions and Inactivation of the Potassium Conductance Induced by Noradrenaline in Smooth Muscle of the Guinea-Pig Vas Deferens. Tohoku J. exp. Med., 1986, 150 (4), 381-389-In order to examine whether any change in cytoplasmic Ca2+ concentration occurs during the noradrenaline (NA)-induced change in the membrane potential, the isometric tension of smooth muscle of the guinea-pig vas deferens was recorded together with membrane potentials using the sucrose-gap method. NA induced a depolarization and an increase in cytoplasmic Ca2+ comprising two (initial and second) phases. In time courses, the depolarization and the second phase of the increase in cytoplasmic Ca2+ were similar. Only at the phase around the onset of the depolarization, the membrane conductance was increased but then, it was decreased. Lowering the external Ca2+ concentration and application of D600 suppressed all of the NA-induced responses. In addition, D600 diminished the size of the NA-induced decrement in the membrane conductance. The present results suggest that the initial phase of the increase in the cytoplasmic Ca2+ is due to the influx of Ca2+ and the second phase, probably to the release of Ca2+ from some intracellular stores. It was discussed that the release of Ca2+ might occur at the internal surface of the membrane, and the membrane might be left less permeable to K+ as a result.View full abstractDownload PDF (885K) -
HIROSHI SATO, MASAYUKI ITO, TATSUYA ABE, NOBUKO KUMANO, MASAKICHI MOTO ...1986 Volume 150 Issue 4 Pages 391-399
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSATO, H., ITO, M., ABE, T., KUMANO, N., MOTOMIYA, M. and KONNO, K. Antitumor Activity of Lipopolysaccharide in Tumor-Bearing Mice Pretreated with BCG. Tohoku J. exp. Med., 1986, 150 (4), 391-399-The antitumor activity of lipopolysaccharide (LPS) was investigated in BCG-treated mice. C3H/He mice and CDF1 mice were injected with BCG and then were inoculated with syngeneic mouse hepatoma MH134 and mastocytoma P815 respectively. Hemorrhagic necrosis and retarded growth of tumor were observed after an intravenous (i.v.) injection of LPS, when tumor cells had been inoculated subcutaneously (s.c.). However an intraperitoneal (i.p.) injection of BCG plus LPS did not increase the mean survival time of mice that had been inoculated with tumor cells i.p. Sera from mice that had been treated with BCG plus LPS i.v. were cytotoxic for cultured tumor cells. These results seemed to indicate that growth-inhibitoryeffects of LPS on tumors inoculated s.c. were mediated by a humoral factor.View full abstractDownload PDF (798K) -
SHIGERU ASAKI, NORIO ISHII, TOSHIAKI NISHIMURA, SHUICHI OHARA, NORIO Y ...1986 Volume 150 Issue 4 Pages 401-406
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSASAKI, S., ISHII, N., NISHIMURA, T., OHARA, S., YAMAGUCHI, N. and TAMURA, T. New Bioptic Method for Submucosal Tumor of the Gastrointestinal Tract. Tohoku J. exp. Med., 1986, 150 (4), 401-406-Biopsy for the submucosal tumor has not been established and it is extremely rare that tumor tissues are obtained by usual biopsy forceps except in cases showing ulceration. Moreover, tumor tissues from the ulcerated site often showed degeneration or necrosis, and are difficult for pathohistological diagnosis. In addition, the biopsies are often associated with bleeding. We have developed a new biopsy method. Briefly, a small amount of pure ethanol is injected topically into the surface of submucosal tumor to produce a small ulcer for biopsy, and several days later a biopsy specimen is obtained from the exposed tumor at the ulcer bottom. We performed this method for 12 lesions in 12 cases. In 64 of 100 bioptic specimens, tumor tissue was obtained and in all cases pathohistological diagnosis could be established. We believe this method will be important to determine the indication of endoscopic treatment or surgical operation, and for follow up observation.View full abstractDownload PDF (3583K) -
KOROKU OTOKIDA, MOTOYUKI NAKAMURA, MAKOTO TAKEDA, TOSHIHARU ITO, MASAT ...1986 Volume 150 Issue 4 Pages 407-415
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOTOKIDA, K., NAKAMURA, M., TAKEDA, M., ITO, T., KATO, M., MASUDA, T. and TASHIRO, A. Significant Regression of the Tumor Combined with the High Level of Plasma Cortisol by Low-Dose Administration of O, p'-DDD in a Case with Cushing's Syndrome Caused by Adrenocortical Carcinoma. Tohoku J. exp. Med., 1986, 150 (4), 407-411-The effect of o, p'-DDD on a functioning adrenocortical carcinoma is described. A 52-year-old woman, who had a huge adrenocortical carcinoma and pulmonary metastasis, was treated by a low-dose administration of o, p'-DDD, which reduced the tumor size. The excretion of urinary 17-OHCS was decreased, whereas plasma cortisol was not decreased but rather increased. This was considered to be caused that o, p'-DDD functioned as a cytotoxic factor which did not improve the states of hyperkalemia, hyperglycemia and hypertension. This suggested that monitoring to measure multiple steroids in addition to plasma cortisol and urinary 17-OHCS was useful for recognizing the general conditions of patients with adrenocortical carcinoma receiving o, p'-DDD therapy.View full abstractDownload PDF (3989K) -
SHIGETOSHI CHIBA, MIYOKO TSUKADA1986 Volume 150 Issue 4 Pages 417-426
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSCHIBA, S. and TSUKADA, M. Angiotensin II-Induced Tachyphylactic Constrictions in Isolated and Perfused Canine Mesenteric Arteries. Tohoku J. exp. Med., 1986, 150 (4), 417-426-The stainless steel cannula inserting method was used to investigate the effects of angiotensin II on isolated and perfused dog mesenteric arteries with and without indomethacin treatment, or with and without intraluminal saponin-treatment which removed the endothelium. In non-treated preparations, angiotensin II was intraluminally administered repetitively at 10, 20, 30 or 60min inervals. Each period of angiotensin II causes tachyphylaxis. The order of degree of tachyphylaxis was 10>20>30>60min intervals. Relatively large doses much readily caused tachyphylaxis. Tachyphylaxis was not affected by phentolamine-treatment preparations. In indomethacin (5mg)-treated preparations, the angiotensin II-induced tachyphylaxis was significantly less than that in non-treated ones. In small doses of angiotensin II, tachyphylaxis did not appear in the majority of cases in indomethacin-treated preparations. In deendothelial preparations which were induced by intraluminal administration of saponin (1 and 3mg), the tachyphylaxis was induced similar to non-saponin treated preparations. It is concluded that 1) prostaglandin may partially participate in induction of angiotensin-induced tachyphylaxis, 2) the prostaglandin may be synthesized in the inside of vasculature, and 3) a large dose of angiotensin II induces tachyphylaxis unrelated to production of prostaglandin.View full abstractDownload PDF (931K) -
YASUHISA WAKABAYASHI, TAKAO MISHINA, FUMIAKI MARUMO, RHUICHI KIKAWADA1986 Volume 150 Issue 4 Pages 427-433
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSWAKABAYASHI, Y., MISHINA, T., MARUMO, F. and KIKAWADA, R. Two Cases of Saline-Responsive Metabolic Alkalosis Associated with High Urinary Chloride Concentrations. Tohoku J. exp. Med., 1986, 150 (4), 427-433-Two particular cases of saline-responsive metabolic alkalosis associated with high urinary chloride concentrations were documented. In the first case, pseudoaldosteronism with long term glycylrrhetinic acid ingestion, and in the second case, probably salt losing nephropathy with extracellular fluid volume contraction were the putative causes of metabolic alkalosis, respectively. Sodium chloride loading with saline (0.9% NaCl) infusion to these patients corrected the accompanying hypopotassemic metabolic alkalosis. Recent trends in the pathophysiology of both saline-responsive and saline-resistant metabolic alkalosis were discussed.View full abstractDownload PDF (728K) -
NOBUMICHI ICHIKAWA1986 Volume 150 Issue 4 Pages 435-446
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSICHIKAWA, N. Study on Monoamine Metabolite Contents of Cerebrospinal Fluid in Patients with Neurodegenerative Diseases. Tohoku J. exp. Med., 1986, 150 (4), 435-446-Main monoamine metabolities of cerebrospinal fluid (CSF), i.e., 3-methoxy-4-hydroxyphenylglycol (MHPG), 5-hydroxyindoleacetic acid (5-HIAA), homovanillic acid (HVA), were measured in 74 patients with neurodegenerative diseases, in 30 normal control subjects and in 16 diseased control subjects without neurodegenerative diseases. The symptoms of patients were scored according to an original scale. It was suggested by statistical study including multiple regression analysis that there might be significant relationship between CSF-MHPG level and extrapyramidal symptoms, or autonomic symptoms, and between CSF-5-HIAA level and extrapyramidal, or cerebellar symptoms, and between CSF-HVA level and extrapyramidal, pyramidal, or cerebellar symptoms. It was also suggested that the severer the symptoms of patients with Parkinson's disease, Shy-Drager syndrome, or olivopontocerebellar atrophy became, the lower the levels of monoamine metabolities of CSF became. The activity of peripheral catecholamine metabolism might influence the CSF-MHPG level because of the significant positive correlations between CSF-MHPG level and urine noradrenaline or vanillylmandellic acid level. The level of MHPG was high in patients with severe amyotrophic lateral sclerosis or hereditary spinocerebellar degeneration possibly because of stress with respiratory dysfunction.View full abstractDownload PDF (1035K) -
RYUICHI NAKAMURA, ICHIRO TSUJI1986 Volume 150 Issue 4 Pages 447-453
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNAKAMURA, R. and TSUJI, I. Effect of Antispastic Drugs on Rapid Force Generation of Spastic Muscle. Tohoku J. exp. Med., 1986, 150 (4) 447-453-The effect of antispastic drugs, dantrolene sodium and baclofen, on the initiation of rapid knee extension was examined in 8 normal subjects and 8 patients with spastic hemiparesis. Three biomechanical variables, the latency between EMG onset of the rectus femoris and rise of tension (TLT), that between the rise of tension and the maximum tension of knee extension (FTmax) and the maximum tension attained (Fmax), were measured before and after administration of one of the drugs. Dantrolene sodium increased TLT, but did not change FTmax or Fmax. The increase of TLT was remarkable in the normal subjects and the non-affected side, compared to the affected side of the patients. Baclofen did not modify TLT or FTmax, but decreased Fmax. The decrease of Fmax was definite in the normal subjects while it was negligible in the affected side of the patients. Clinical implications and pathophysiological mechanisms of these results were discussed.View full abstractDownload PDF (733K) -
SHIGERU TSUCHIYA, NAOKO MINEGISHI, MASAYOSHI MINEGISHI, TETSUO SATO, T ...1986 Volume 150 Issue 4 Pages 455-465
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSTSUCHIYA, S., MINEGISHI, N., MINEGISHI, M., SATO, T. and KONNO, T. Adaptation of a Human Monocytic Leukemia Cell Line (THP-1) in a Protein- Free Chemically Defined Medium. Tohoku J. exp. Med., 1986, 150 (4), 455-465 -We have adapted a human monocytic leukemia cell line [THP-1c12(+)] so that it can proliferate in a completely protein-free chemically defined medium of an equal mixture of Dulbecco's modified Eagle's minimum essential medium and Ham's F12. This cell line was designated as THP-1c12(-). When a sufficient number of THP-1c12(-) cells was seeded in culture, the maximum cell density after 6 days of culture was 1×106/ml with a doubling time of 30 hr. Transferrin showed a slight stimulative effect on the growth of THP-1c12(-) cells, but insulin did not. The surface profile of THP-1c12(-) was the same as that of THP-1c12(+), which possessed Mol. Mo5, LeuM3, My9 and Ia-like antigens as well as a large number of Fc receptors. Phagocytic ability with respect to IgG-coated sheep red blood cells was evident in both THP-1c12(+) and THP-1c12(-) cells. A small percentage of THP-1c12(-) cells retained the ability to reduce nitroblue tetrazolium when stimulated with 12-tetradecanoyl-phorbol-13-acetate. Culture supernatant from THP-1c12(-) stimulated the incorporation of 3H-thymidine into its own cells, suggesting an autocrine mechanism by which the growth of THP-1c12(-) cells is induced in a protein-free medium.View full abstractDownload PDF (909K) -
JIRO HOZAWA, SHINICHI USAMI, ISAO MORI1986 Volume 150 Issue 4 Pages 467-474
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSHOZAWA, J., USAMI, S. and MORI, I. Validity of Ewald's Law in the Horizontal Semicircular Canals and the Vestibular Nuclei: Electron Microscopic Observation and Quantitative [14C]-2-Deoxy-D-Glucose Study. Tohoku J. exp. Med., 1986, 150 (4), 467-474-Two series of experiments were performed by using a total of 36 guinea pigs. The 1st series was to compare the influence of rotatostimulation on the bilateral cristae ampullare. After repeating the rotatostimulation with the cupulometric mode (the terminal turning velocity: 450°/sec) for 48hr, ultrastructural changes of sensory cells were found in 9 of 17 animals with transmission electron microscopy. They showed stronger influences on the ampullopetal flow side with the exception of one animal. In the 2nd series, the difference in excitability between the bilateral vestibular nuclei was investigated on 6 guinea pigs which were exposed to the clockwise constant angular acceleration of 1°/sec2 for 8min. The autoradiographic study with [14C]-2-deoxy-D-glucose (the 2-DG) revealed that the rotation-induced glucose uptake was increased significantly in the superior and the medial vestibular nuclei of the ampulloptal flow side. The results of the two series of experiments suggest the predominance of the ampullopetal effect not only in the horizontal semicircular canals but also in the vestibular nuclei.View full abstractDownload PDF (3276K) -
TOSHIHIKO IIJIMA, NORIO TAIRA1986 Volume 150 Issue 4 Pages 475-480
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSIIJIMA, T. and TAIRA, N. Modification by Nicorandil and Carbachol of the Inward Calcium Current in Single Cells of the Guinea-Pig Heart. Tohoku J. exp. Med., 1986, 150 (4), 475-480-Effects of nicorandil and carbachol on the inward calcium current were studied in single heart cells isolated from the guinea-pig using a collagenase dispersion method. The inward calcium current was obtained in the absence of outward potassium currents by intra- and extra-cellular perfusion of the cell with Cs+-solutions. Nicorandil (0.3mM) failed to modify the inward calcium current in the atrial cell. In the ventricular cell the inward calcium current was increased by isoproterenol (1μm). The augmentation of the inward calcium current was abolished by carbachol (1μm) but was not changed by nicorandil (0.3mM). From these results it can be concluded that nicorandil has no direct effect on the inward calcium current even when the inward calcium current is previously augmented by isoproterenol.View full abstractDownload PDF (502K) -
SETSUKO KITAOKA, MASAHIKO TERASAWA, EISUKE GOTO, TATSUO MIYAJI, SHIGER ...1986 Volume 150 Issue 4 Pages 481-482
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKITAOKA, S., TERASAWA, M., GOTO, E., MIYAJI, T., TSUCHIYA, S. and KONNO, T. Trimethoprim Interference in Methotrexate Assay by an Enzyme Inhibition Assay Kit. Tohoku J. exp. Med., 1986, 150 (4), 481-482-Spurious methotrexate (MTX) concentrations in sera and cerebrospinal fluids from leukemia patients who were given trimethoprim (TMP) were estimated using an MTX assay kit which is based on its inhibition of dihydrofolate reductase. The interference of TMP with MTX was confirmed in the assay system. A concentration of 0.17μg/ml of TMP gave a value for an apparent MTX of 10μM.View full abstractDownload PDF (419K) -
NOBUKO KUMANO, SADAHIRO KOINUMARU, SHUJI SUZUKI, TETSUKO ISHIKAWA, KOT ...1986 Volume 150 Issue 4 Pages 483-484
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKUMANO, N., KOINUMARU, S., SUZUKI, S., ISHIKAWA, T., OIZUMI, K. and KONNO, K. Tumor Histology and the Immunoregulatory T Lymphocyte Subsets in Lung Cancer Patients. Tohoku J. exp. Med., 1986, 150 (4), 483-484-The immunoregulatory T lymphocyte subsets (CD4+, CD8+) in 39 patients with primary lung cancer were analyzed as for the possible correlation with tumor histology. Compared to the healthy controls (n=36), cancer lymphocytes were generally characterized by a higher proportion of CD8+ subset (p<0.01) with no difference in either CD4+ subset or CD3+ cells. There was, however, a significant difference (p<0.05) between one extreme of epidermoid carcinoma with the highest CD8+ and the lowest ratio (CD4+/8+) and the other end of adenocarcinoma with the lowest CD8+ and the highest ratio. Large cell and small cell carcinomas were of the intermediate values. A spectrum of the subset marker profiles was thus found in association with tumor histology. A diversity in the host immune status was suggested in primary lung cancer, although clinical implication remains to be clarified.View full abstractDownload PDF (419K)
- |<
- <
- 1
- >
- >|