1. A dialyzable KIK factor (Prep. 1) was isolated from cancerous ascitic fluids and a corresponding mucoprotein (Prep. 1') from the noncancerous in electrophoretically homogeneous state, and their properties were examined.
2. Prep. 1 and 1' both were composed of galactosamine, glucosamine, galactose, glucose, mannose, L-fucose and xylose and/or lyxose.
3. Prep. 1 differed from the undialyzable factor from the same source in quantitative-analytical figures of the sugar components, optical rotation, electrophoretic mobility and solubility and from Prep. 1' in protein and sialic acid contents, optical rotation and electrophoretic mobility.
4. Prep. 1 was not deprived of the anemia-inducing potency by heating with water at 100°C, agitation with glacial acetic acid and boiling with alcohol.
5. The anemia-inducing potency of Prep. 1 was not damaged by heating at 100°C with 0.1
N acetic acid, notwithstanding that parts at least of L-fucose and of sialic acid were cleft off thereby. The potency was destroyed when the substance was heated with slightly alkalinized physiological saline, 1
N acetic acid and 0.1
N hydrochloric acid. Judging from the chemical findings in these cases, all the molecules of galactose look to be indispensable for the activity.
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