Serum zinc and copper concentrations of 156 healthy children (6-12 years old) were measured by the atomic absorption spectrophotometric method. The average serum zinc concentration was 92±13 üg/100ml (mean±s.D.) with a range of 62-127 üg/100 ml. No statistically significant age or sex differences were found in serum zinc levels. The mean serum copper level was 109±17 üg/100ml with a range of 70-164 üg/100ml. Average serum copper concentration in younger age groups (6-9 years old) was higher than that in older age groups (10-12 years old). The highest value of mean serum copper level was observed in the group of 8-year-old children. No sex differences in serum copper levels were found in all the age groups.
An 18-year-old mentally and physically retarded boy, suffering from episodes of anorexia, vomiting, coma and convulsion which have been severer with advance in age, had periodic hyperammonemia, hyperlysinemia and homocitrullinuria. Blood cell arginase activity of the patient on normal diet was markedly reduced after an oral load of Y. -lysine. The oral loading tests of L-lysine revealed hyperammonemia, hyperlysinemia, hyperargininemia, hypercitrullinemia and homocitrullinuria. Etiology of metabolic error of our patient was discussed in reference to lysine-urea cycle.
Three atypical patients with clinical and laboratory findings of Hurler syndrome, but without α-L-iduronidase deficiency, are described. Clinical features included characteristic facies, mental retardation, corneal clouding, dysostosis multiplex, restriction of joint mobility, and hepatosplenomegaly. Excessive amounts of chondroitin sulfate B and heparitin sulfate were excreted in the urine. α-L-Iduronidase activities in leucocytes and liver tissues were within the normal range or somewhat elevated.
A new procedure for radioimmunoassay (RIA) was applied to determine the serum concentration of thyroxine (T4). Mixtures of T4-methylester hydrochloride-bovine serum albumin complex and complete Freund's adjuvant were injected to rabbits to get them immunized. Specific anti- T4 rabbit serum, high in titer, could be obtained 18 months after the start of immunization. RIA for T4 extracted by ethanol from serum was performed by an application of dextran-coated charcoal to separate bound and free 125I-T4. On the other hand, RIA for T4 performed directly in the serum containing 100 mg/ 100 ml of 8-anilino-l-naphthalene-sulfonic acid (ANS) gave a similar standard curve for T4 obtained by the ethanol-extraction method. Serum T, values obtained by the methanol-extraction method were 9.7±3.3, μg/100 ml for normal subjects, 17.8±2.9μg/100 ml for patients with hyperthyroidism and 0.6±0.3 μg/100 ml for those with hypothyroidism. The corresponding figures obtained by RIA in the serum containing ANS were 11.1±2.1, 19.1±3.3 and 0.7±0.7 μg/100ml, respectively. The figures positively correlated respectively to the serum T4. values obtained by the competitive protein-binding analysis method (Tetrasrob-125)
Based on clinicopathological observations of 91 female patients with breast cancer in Miyagi Prefecture, TNM stage, histologic type, grade of infiltrative growth tendency (INF), vessel involvement, lymph node metastasis, nuclear grade and sinus histiocytosis were assessed in relation to prognosis. Follow-up studies indicated that 73.6% of the patients survived more than five years after operation. Among the factors sub-mitted to histologic evaluation, nuclear grade, INF, vessel involvement and lymph node metastasis were apparently correlated to prognosis, while sinus histiocytosis was not significantly related to it, except for patients with positive nodes.
Antecubital venous blood was sampled from stroke patients in the presence of disodium ethylenediamine tetraacetate. Plasma was analyzed for cyclic AMP applying a competitive protein binding method without any special pretreatment. In mild cerebral hemispheric infarction as manifested by moderate hemiparesis and/or dysarthria, plasma cyclic AMP remained in the normal range (8-18 picomoles/ml). In most of the cases with moderate infarction, the cyclic AMP level was distinctly below the normal range several days after the onset of symptoms. However, cyclic AMP remained in the normal range in severe infarction with signs of brain edema, and in two cases with moderately severe symptoms. One of the two cases suffered from later develop-ment of brain edema, and the other revealed a large lesion in brain scintigrams. The sizes of the lesion revealed in brain scintigrams were smaller in the moderate cases and larger in the severe cases, except in one of the cases mentioned above. It appeared that with plasma cyclic AMP levels we could predict the extent of the lesion, and perhaps the subsequent development of impending brain edema in a few days after the onset of cerebral infarction. In moderate cases of cerebral hemorrhage, judged from the consciousness, cyclic AMP decreased to a subnormal level 2-4 days after the onset. In severe cases it remained in the normal range. Subarachnoid hemorrhage showed significantly elevated cyclic AMP levels in the early stage.
Thirteen patients with acromegaly were subjected to the examination of autonomity in growth hormone (GH) secretion. TRH (500μg iv), arginine (0. 5g/kg of body weight iv infusion), LH-RH (100μg iv) and L-dopa (500mg orally) were administered, and plasma GH was measured. Among them, 11 patients showed some response in plasma GH to at least one agent, but the other 2 cases showed no response to any of the above 4 agents. In the former 111 cases, the patients were regarded as belonging to the less autonomous type and in the latter 2, to the more autonomous type in GH secretion. Six cases (4 cases of the less autonomous and 2 cases of the more autonomous type) received an admin-istration of 500μg of synthetic somatostatin parenterally. Following administra-tion of somatostatin, the patients of both types showed significant GH decrease, although GH decrease in the more autonomous type was smaller than that of the less autonomous type. These results would suggest that there might be no acromegalics secreting GH from the pituitary with complete autonomity, and the difference of autonomity in acromegalic patients might depend either on the difference in sensitivity and/or the number of receptors in the pituitary rather than those in hypothalamus to exogenous stimuli.
It has been known that the agglutinability of human red cells is changed or enhanced by treatments with proteolytic enzymes or neuraminidase. In this paper, the serological properties of agglutinogens developed by proteolytic enzymes (bromelin, ficin, papain, trypsin and pronase) and neuraminidase are investigated by using antisera to trypsin- and neuraminidase-treated red cells. The adsorptions of the antiserum to trypsinized red cells with the cells treated with each of the proteolytic enzymes showed that the agglutinogens uncovered by bromelin, ficin and papain were different from those by pronase and trypsin. It was demonstrated that pronase was the most effective enzyme to uncover the agglutinogen located on deeper site of red cell membrane. This was confirmed by the agglutination with the test cells treated twice with two kinds of the enzymes. The reactions of the antiserum to neuraminidase-treated red cells treated with six kinds of the enzymes indicated that the agglutinogens developed by neuraminidase resembled those by bromelin, ficin and papain more than to those by trypsin and pronase. Blood
A case of membranous glomerulonephritis with initial proliferative changes accompanied by nephrotic syndrome was followed up for seven years. With three serially obtained renal biopsy tissues, the regression of the glomerular membranous alterations was confirmed after the remission of nephrotic syndrome. The diagnosis of lupus nephritis was not conclusive but was suggestive. The resolution of the basement membrane seemed to start structurally at the outer, epithelial surface of the membrane with the formation of basement membrane material. The “microtubular structures” in the glomerular endothelial cytoplasm were continuously observed in three renal tissues obtained serially for five years, regardless of the clinical remission and of glomeru-larstructural resolution.
During dietary treatment of a case of maple syrup urine disease, it was found that abnormal EEGs were observed when serum levels of leucine were abnormally high while those of valine and isoleucine were normal, and also when sernln levels of valine and isoleucine were abnormally high while serum leucine levels were normal.
The effect of dibutyryl 3', 5'-cyclic monophosphate (D-c AMP) on the neuromuscular transmission was studied in a patient with myasthenia gravis during anesthesia. D-cAMP caused a slight increase in single twitch tension, and an initial transient decrease in tension which resulted from the trains of 2 Hz stimuli disappeared after D-cAMP. The finding of the present study suggests that D-cAMP has an anti-fatigue effect in patients with myasthenia gravis.
Penial scintiphoto-images were obtained by using 99m Tc-labeled autologous red blood cells in three functionally impotent patients. The cavernosum penis was clearly pictured disclosing no organic abnormalities in these three patients' penises. This method enables one to present clear macroscopic images of the cavernosurn penis without causing pain to the patient, and it may be recommended for clinical use as an alternative to cavernosography.