YOSHIE, O. and ONO, Y. Frequencies of Specific Antibody-Producing Cellsfor Sheep Red Blood Cells and for Phosphorylcholine in Human Tonsillar BLymphocytes Transformed by Epstein-Barr Virus. Tohoku J. exp. Med., 1982, 137 (3), 233-244-The frequencies of transformed B cells and of transformed specific antibody-producing cells in human tonsillar lymphocytes infected with Epstein-Barr virus (EBV) were determined by limiting dilution analyses. The latter was corrected by the former and the frequencies of specific antibody-producing cells in EBV-transformed B cell populations were obtained. The frequencies of specific B cells for sheep red blood cells were calculated to be one in 500 to 2, 000 EBV-transformed tonsillar B cells obtained from four donors, while those of specific B cells for a hapten, phosphorylcholine, were one in 700 to 5, 000. The method may be useful to define the repertoire of specificities in the human B cell populations.
KAMEYAMA, J., SASAKI, I., IMAMURA, M., NAITO, H. and SATO, T. SurgicalTreatment for Crohn's Disease, with Special Reference to Operative Procedures andTheir Relationship to Recurrence. Tohoku J. exp. Med., 1982, 137 (3), 245-251-Of 14 patients with Crohn's disease, 12 to whom operation was performed at our department were studied with regard to operative procedures and their relationship to recurrence. The following results were obtained: 1) Resection of the intestinal lesions was performed in all patients. The recurrence rate was as high as 42% (5 of 12 cases). 2) If the length of the normal intestine resected was more than 10cm, there was no correlation between the length of the resection and recurrence of the disease. 3) The postoperative recurrence rate tended to decrease by means of regional lymph nodes dissection. From these results, it is concluded that the recommendable operative procedures for Crohn's disease are intestinal resection including the normal intestine more than 10cm in length and regional lymph nodes dissection.
IKENO, T., MINAMI, R., TSUGAWA, S. and NAKAO, T. Acidic Glycosaminoglycans and Gangliosides in the Brains from Four Patients with Genetic Mucopolysaccharidosis. Tohoku J. exp. Med., 1982, 137 (3), 253-260-Acidic glycosaminoglycans (GAGs) and gangliosides were analyzed in the brains from control fetuses, fetal Hurler syndrome, control children and three patients affected by genetic mucopolysaccharidosis (Hurler, Hunter and Morquio syndromes). GAGs contents in the brains from patients with Hurler and Hunter syndromes were approximately 1.5-3.0-fold greater as compared with those from controls, and most of the GAGs were much more degraded than those from controls. Dermatan sulfate (DS), which was not identified in the control brains, comprised about 20-40% of the total GAGs. On the other hand, GAGs content and molecular weight distribution in the brain from the patient affected by Morquio syndrome were similar to those in the control brains. GAGs content in the brain from fetal Hurler syndrome was also 2.0-fold greater and DS, which was not detected in the control fetal brains, comprised 1.6% of the total GAGs. However, the molecular weight distribution of the GAGs was similar to those of the control fetal brains. The total amount of the brain gangliosides in all patients assayed here was similar to those in the control brains. However, a greater amount of GM1- and GM2-gangliosides was observed in the brains from patients with Hurler and Hunter syndromes.
CAMPBELL, M.G., COPP, E.P., JAMES, J.L., KEENAN, J., MERRY, P.H. and RUDOLFER, S.M. A Comparison of Normal Limits Arising in Nerve ConductionStudies. Tohoku J. exp. Med., 1982, 137 (3), 261-267-A detailed study has been carried out on the influence of various factors, including sex of the patient and side examined, on normal values in peripheral nerve conduction studies. Patients were selected from three clinics, thereby enabling comparisons not only to be made within clinics but also between clinics.
SHIMAMOTO, K., CHAO, J. and MARGOLIUS, H.S. A Method for Determinationof Human Urinary Inactive Kallikrein (Prekallikrein). Tohoku J. exp. Med., 1982, 137 (3), 269-274-Inactive kallikrein (prekallikrein) in human urine was discovered recently. A more accurate method to measure this zymogen and total kallikrein is described. With trypsin pretreatment, it was found that 35.3±2.7% of total urinary kallikrein activity was prekallikrein. The results are supported by a new direct radioimmunoassay which measures predominantly active kallikrein.
HARA, K., KASHIWAMATA, S., OGASAWARA, N., OHISHI, H., NATSUME, R., YAMANAKA, T., HAKAMADA, S., MIYAZAKI, S. and WATANABE, K. A Female Caseof the Lesch-Nyhan Syndrome. Tohoku J. exp. Med., 1982, 137 (3), 275-282-The classical Lesch-Nyhan syndrome has the deficiency of hypoxanthine-guanine phosphoribosyltransferase (HGPRT) activity as the result of mutation in the structural gene for the enzyme located on the X chromosome and is believed to be of X-linked recessive or sex-limited mode of inheritance. This is the first report of a girl who showed typical clinical features and biochemical characteristics of the classical Lesch-Nyhan syndrome. Her mother was not a heterozygote for a deficiency of HGPRT. Possible genetic mechanisms responsible for this case were discussed.
DEMURA, R., SUDA, T., WAKABAYASHI, I., ONO, M., JIBIKI, K., ODAGIRI, E., DEMURA, H. and SHIZUME, K. Plasma LH, FSH and TSH Responses to theSynthetic Enkephalin Analog (FK 33-824) in Normal Subjects and Patients withPituitary Diseases. Tohoku J. exp. Med., 1982, 137 (3), 283-287-D-Ala, Mephe, Met, enkephalin (Sandoz FK 33-824), 1.0 or 0.5mg, was administered by i.v. infusion to normal subjects, patients with acromegaly and hyperprolactinemia due to pituitary adenoma, and patients with pituitary dwarfism. FK 33-824 induced no significant change in plasma level of LH, FSH or TSH in any of the subjects studied. These results suggest a lesser role of opioid regulation in the release of above pituitary hormones compared with that in GH, PRL and cortisol.
FUJIMURA, Y., KAWAMURA, M. and NARUSE, H. Simultaneous QuantitativeEstimation of Galactose-1-Phosphate and Galactose in Blood for the Diagnosis ofGalactosemia. Tohoku J. exp. Med., 1982, 137 (3), 289-295-A new microfluorometrical simultaneous assay method of galactose-1-phosphate and galactose in blood discs was devised by use of alkaline phosphatase and β-galactose dehydrogenase. Our method statistically corresponded well with the Kirkman's method. It can detect 1×10-10 mole of minimal concentration of galactose-1-phosphate and galactose in one blood disc paper (3mm in diameter), and this means the sensitivity of assay of galactose-1-phosphate was 0.1mg%. Assay range in our method was very broad (0-2mM or 0-10mM). The accuracy and reproducibility of galactose-1-phosphate assay were 3.4±0.1mg%, 8.0±0.4mg% or 15.1±0.6 mg%. Mean values of galactose-1-phosphate and galactose in blood of normal infants were 0.8mg% and 0.3mg%, respectively. We applied this method to mass screening of galactosemia and could accurately distinguish many positive and false positive cases detected by Paigen's and Beutler's methods. This method gave us an easy and accurate assay system for the diagnosis of uridyl transferase and galactokinase deficiencies.
YAMAMOTO, R. and SUZUKI, T. Decreased Membrane Fragility of MouseErythrocytes by Small Dose of Methylmercury and Its Restoration by Coadministered Selenite. Tohoku J. exp. Med., 1982, 137 (3), 297-303-Female IVCS mice were fed methylmercury at a low dose level (10nmoles/g feed) with coadministration of selenite (0, 8, 20 or 50nmoles/ml of drinking water) for one week or two weeks. Mice fed methylmercury alone showed the decreased fragility of erythrocytes membrane when compared with the non-treated control. Coadministration of selenite produced such a change to smaller extents than methylmercury treatment alone. Mercury levels in the blood varied according to the dose level of selenite (the highest level was found in methylmercury alone group).
WATANABE, T., MIYASAKA, M., KOIZUMI, A. and IKEDA, M. RegionalDifference in Sodium Chloride Content in Home-Made and Store-Bought Preparationsof Miso Paste. Tohoku J. exp. Med., 1982, 137 (3), 305-313-Miso, a fermentation product of salted soya beans and rice (or soya beans and barley), is one of the leading sources of sodium chloride intake for Japanese people. Sodium chloride contents in 973 home-made preparations collected from 39 Regions in 20 Prefectures in Japan showed wide variation from 9.1 to 18.2% on the Region average, while the levels in store-bought samples were rather low and constant at 10 to 12%. When classified by Regions or Prefectures, positive and significant correlations were observed between indices of sodium chloride intake via miso (i.e., sodium chloride in home-made miso, or frequency of miso soup intake) and death rate indices for cerebrovascular diseases.
SUZUKI, T., KASHIMURA, S. and UMETSU, K. Experimental Model of SuddenCardiac Death from Unexplained Cause. Tohoku J. exp. Med., 1982, 137 (3), 315-323-To find a possible explanation for sudden cardiac death from unexplained cause, an experimental model was made with rabbits. The rabbits were fed an atherogenic diet for 2 and 4 months without precordial x-irradiation. Though the coronary sclerosis in the hearts of these rabbits was only slight or not detectable, the ventricular premature contraction threshold (VPCT) and the ventricular fibrillation threshold (VFT) decreased by about 50% and 60% of those in the control animals, respectively. The investigation of isozyme patterns of LDH, GOT, MDH and CPK in the hearts revealed that the changes of these isozyme patterns were almost identical with the results from the human hearts of sudden cardiac death from unexplained cause. From these results, the rabbits fed an atherogenic diet without precordial x-irradiation can be an experimental model of sudden cardiac death from unexplained cause.
NAKAZAWA, I. and IWAIZUMI, M. A Correlation between Cancer Metastasesand the Fluidity of Cancer Cell Membrane. Tohoku J. exp. Med., 1982, 137 (3), 325-328-Male Donryu rats weighing from 100 to 120g were transplanted with AH100B, AH66F or Yoshida sarcoma (YS) cells intraperitoneally. After collecting ascites, while not bloody, the cancer cells were washed three times with Dulbecco's phosphate buffered saline (PBS) without Ca++ and Mg++ (pH 7.2). Then, each sediment was suspended in PBS mentioned above. Each suspension in which 10 million of cancer cells were contained was violently vibrated with a definite amount of 5-doxyl stearic acid and spin labeling of cancer cell membrane was done. Furthermore, each specimen thus obtained was subjected to measurements of ESR spectra and the order parameter was determined from the spectra. The order parameter of AH100B was the highest and that of YS the lowest at each temperature measured from 5°C to 35°C. YS cells have been reported to be found in blood stream within 24hr, AH66F cells in a day and AH100B cells in 10 days or more after intraperitoneal inoculation. The results obtained here suggest that the accelerated fluidity of cancer cell membrane is related with the cancer metastasis.
HAYASAKA, K., METOKI, K., SATOH, T., NARISAWA, K., TADA, K., KAWAKAMI, T., MATSUO, N. and AOKI, T. Comparison of Cytosolic and MitochondrialEnzyme Alterations in the Livers of Propionic or Methylmalonic Acidemia: A Reduction of Cytochrome Oxidase Activity. Tohoku J. exp. Med., 1982, 137 (3), 329-334-The activities of mitochondrial, cytosolic and microsomal enzymes in liver specimens obtained from three patients with propionic or methylmalonic acidemia were compared with those of control patients who had died from unrelated causes. Only the activity of cytochrome oxidase (mitochondrial enzyme) was significantly reduced in the patients of propionic acidemia and methylmalonic acidemia who were in the state of metabolic acidosis; in two patients the activity was less than 30% of that in controls, but in the other patient of propionic acidemia, who was under the treatment with a low protein diet (0.8g/kg/day), the activity was 50% of that in controls. The metabolites of branched chain amino acids (tiglic acid, propionic acid, methylmalonic acid, succinic acid, tiglyl-CoA and propionyl CoA) exhibited no inhibitory effect on the cytochrome oxidase activity of the sonicated rat liver mitochondria. The reduction of cytochrome oxidase activity found in these organic acidemias may be caused secondarily by some unknown mechanism.
TANNO, Y., ARAI, S. and TAKISHIMA, T. Iron-Containing Proteins AugmentResponses of Human Lymphocytes to Phytohemagglutinin and Pokeweed Mitogenin Serum-Free Medium. Tohoku J. exp. Med., 1982, 137 (3), 335-343-The effects of various iron-containing compounds on the responses of human peripheral lymphocytes to phytohemagglutinin (PHA) and pokeweed mitogen (PWM) were studied in serum-free medium supplemented with bovine serum albumin. Hemoglobin, transferrin and ferritin enhanced the incorporation of 3H-thymidine into DNA after PHA-stimulation of lymphocytes, while hemin, iron metal powder, ferrous sulfate, chromium powder, and zinc sulfate have little effect. The response to PWM, measured by plaque formation, was enhanced only by transferrin. Desferrioxamine, a chelating agent specific for ferric iron, completely removed these augmentative effects. The results indicate that iron-containing proteins influenced the responses of lymphocytes to stimulation by PHA and PWM in serum-free medium.
KOIWA, Y., HASHIGUCHI, R., ISOYAMA, S., SATOH, S., SUZUKI, H., TAKISHIMA, T. and NI-INO, H. The Acoustic Characteristics of the Left Ventricle. Tohoku J. exp. Med., 1982, 137 (3), 345-346-A new method was developed to describe the instantaneous resonance curve (IRC) of the left ventricle (LV), which represents the acoustic characteristics and instantaneous viscoelastic properties of LV. This method was evaluated using coronary-perfused isolated canine LV preparations
YOSHIDA, T., HATTORI, Y., TAKASHIMA, Y. and NODA, K. Serum QxytocinConcentrations in Women under Pseudopregnant Therapy. Tohoku J. exp. Med., 1982, 137 (3), 347-348-The serum oxytocin concentrations in the pseudopregnant women were investigated. Since the beginning of the administrations of estrogen and gestagen, the serum oxytocin level had increased gradually and it reached at the same level as in the third trimester pregnant women in the 12th week of administration. The mechanism of increase of oxytocin in pregnant women is discussed.