LEE, M., HIROKAWA, M., KITABAYASHI, A., MIURA, A.B., ITOH, H. and TASHIMA, Y. Induction of 73-kDa and 90-kDa Heat Shock Protein Expression inHL60 Cells during Differentiation. Tohoku J. Exp. Med., 1995, 176 (4), 191-202 - We investigated the expression of heat shock proteins HSP73 and HSP90 in human promyelocytic line HL60 during their differentiation induced by phorbor ester and all trans retinoic acid (ATRA). Polyclonal antibodies which had been raised against HSPs purified from bovine brains were used to detect human HSPs. Semiquantitation of HSPs was performed by immunoblotting. Induction of monocytoid and granulocytoid differentiation with phorbor ester and ATRA was accompanied by increased expression of HSP73 and HSP90 with distinct kinetics. Increased expression of both HSPs was confirmed by immunocytochemical studies. Our results suggest that HSP73 and HSP90 may be involved in differentiation of HL60 cells.
ONODERA, H., UKAI, K. and MINAMI, Y. Hepatocellular Carcinoma Caseswith Five-Year Survival and Prognostic Factors Affecting the Survival Time. Tohoku J. Exp. Med., 1995, 176 (4), 203-211 - Between 1980 and 1994, 178 patients were confirmed to have hepatocellular carcinoma (HCC) in our hospital. The 5-year survival rates in patients with HCC of stage I, II and IVA were 38.1%, 31.8% and 3.9%, respectively. No patient in stage III or IVB survived for more than four years. The 5-year survival rates of the patients treated by hepatic resection, ultrasonically guided percutaneous ethanol injection and transcatheter hepatic arterial embolization were 53.7%, 38.7% and 13.5%, respectively. The logrank test showed a significant difference in cumulative survival rates obtained in patients with HCC according to the tumor stage (p<0.001) or principal treatment procedure (p<0.001). Twelve patients survived for more than five years. We employed a Cox's proportional hazards model to estimate the factors significantly affecting the survival time. Variables with statistical significance were the clinical stage (p<0.001), tumor size (maximal tumor diameter) (p< 0.001) and patient's age (p<0.05). Conclusively, patients in the early stage of HCC associated with mild liver cirrhosis have a significantly better chance for long survival.
OTSUJI, E., YAMAGUCHI, Y., KODAMA, T. and TAKAHASHI, T. Application ofMonoclonal Antibodies to Histologic Diagnosis of Gastric Cancers. Tohoku J. Exp. Med., 1995, 176 (4), 213-218 - Histologic diagnosis of poorly differentiated gastric adenocarcinomas by conventional hematoxylin-eosin (HE) staining is often limited by the quality of gastroendoscopic biopsy specimens. In this study we attempted to identify gastric cancer cells in biopsy specimens and ascites of gastric cancer patients using the gastric cancer reactive monoclonal antibodies S202 and S74. Twenty-four of 25 specimens shown to contain cancer cells by HE staining were stained positively by monoclonal antibody S202. In contrast, 3 of 16 specimens that were diagnosed as not including cancer cells by HE staining was stained positively by S202. The possibility that cancer cells in the biopsy specimens were not detected by HE staining cannot be ruled out. Cancer cells in ascites of patients diagnosed with carcinomatous peritonitis by Papanicolaou staining were stained positively by monoclonal antibody S74. From these results, we conclude that monoclonal antibodies S202 and S74 may be useful supplemental agents for the diagnosis of gastric cancer.
KATAGIRI, T., ITO, S., NAKAMURA, H., NAKAGAWA, O., USAMI, A., TANI, N. and SHIBATA, A. Do the Effects of Blood Pressure Control on the Progression ofDiabetic Nephropathy Depend on Disease Stage? Tohoku J. Exp. Med., 1995, 176 (4), 219-225 - To determine whether the control of blood pressure has a consistent effect on the progression of diabetic nephropathy with macroalbuminuria in diabetic patients with differing serum levels of creatinine, we evaluated the relationship between mean blood pressure and the yearly rate of increase in serum creatinine concentration in two groups: one with macroalbuminuria and a serum creatinine concentration less than 2mg/100ml (group 1), and the other with macroalbuminuria and a serum creatinine concentration of 2mg/100ml or more (group 2). Both groups showed a positive relationship between the mean blood pressure and the yearly rate of increase in serum creatinine concentration. However, when the yearly rate of increase in serum creatinine concentration was compared in patients from both groups with matched mean blood pressures of less than 100mmHg, it was greater in patients of group 2 than in patients of group 1. Thus, although a beneficial effect of blood pressure control on the progression of diabetic nephropathy was found in both groups, the effect was less in patients with macroalbuminuria and a serum creatinine concentration of 2mg/100ml or more when mean blood pressure was controlled at less than 100mmHg.
MUNAKATA, A., IWANE, S., TODATE, M., NAKAJI, S. and SUGAWARA, K. Effectsof Dietary Fiber on Gastrointestinal Transit Time, Fecal Properties and FatAbsorption in Rats. Tohoku J. Exp. Med., 1995, 176 (4), 227-238-The gastro-intestinal transit time of food was determined by x-ray fluoroscopy using barium sulfate in rats fed with diets of various dietary fiber contents, and the effects of dietary fiber on the transit time, properties of feces, and fat absorption were examined. In 4- and 16-month-old rats fed with the diet for 3 and 15 month, respectively, the transit time of the cecum and colon in those receiving 20 and 40% wheat bran diets was shortened compared with that in the 0% group. The fecal pellet number and volume increased as the wheat bran content of the diet increased. In another experiments, the daily total fat excretion was found to be the greatest in rats receiving 15% pectin diet, followed by rats receiving 15% cellulose and non-fiber diets, respectively. These results suggest that shortening of the transit time through the cecum and colon with increase of fecal volume and suppression of fat absorption all participate in the mechanism of the inhibitory action of wheat bran on carcinogenesis and on the development of diverticulum of the large intestine.
KÖSE, K., DOGAN, P., ASÇIOGLU, M., ERKILIC, K. and ASÇIOGLU, Ö. Oxidative Stress and Antioxidant Defenses in Plasma of Patients with Behçet'sDisease. Tohoku J. Exp. Med., 1995, 176 (4), 239-248-Plasma myeloperoxidase (MPO) and glutathione peroxidase (GSH-Px) activities, and malondialdehyde (MDA), ceruloplasmin (Cp), transferrin (TF), thiol (SH), selenium (Se) and copper (Cu) levels were determined in patients with Behget's disease (BD), in order to investigate whether the plasma antioxidant defense system is impaired in BD. When compared to controls, plasma MDA, Cp, Cu levels and also plasma MPO activity were significantly higher in patients, whereas plasma TF, SH and Se levels, and also plasma GSH-Px activity were lower in BD patients than those in controls. In addition, there were significant and positive correlations between MDA-Cp, MDA-Cu, MDA-MPO, MPO-Cp, GSH-Px-Se, Cp-Cu, and TF-SH parameters, but negative correlations between MPO-TF and Cp-TF parameters in BD patients. Based on these findings, it is concluded that plasma antioxidant defense system is insufficient and impaired in BD.
TORINUKI, W. Contact Dermatitis to Biperiden and Photocontact Dermatitisto Phenothiazines in a Pharmacist. Tohoku J. Exp. Med., 1995, 176 (4), 249-252 -A case of contact dermatitis to biperiden, an anti-Parkinson agent, and photocontact dermatitis to phenothiazines in a pharmacist was reported. The patient developed eczematous lesions on exposed area after she had worked at a psychiatric hospital for 6 months. She showed positive patch test reaction to Biperiden. In addition, she reacted positively to photopatch testing with ultraviolet A and phenothiazines such as chlorpromazine and perphenazine. To our knowledge, contact dermatitis to Biperiden has not been previously reported in the English literature.