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Volume 172 (1994)
- Issue 4 Pages 295-
- Issue 3 Pages 175-
- Issue 2 Pages 97-
- Issue 1 Pages 1-
Volume 172, Issue 1
Displaying 1-10 of 10 articles from this issue
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HIDEAKI KONO, YOUNG CHIN LIN, MAMORU YAMAGUCHI, FREDERICK P. ZUSPAN, R ...1994 Volume 172 Issue 1 Pages 1-8
Published: 1994
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKONO, H., LIN, Y.C., YAMAGUCHI, M., ZUSPAN, F.P., O'SHAUGHNESSY, R.W., LEE, A.C., FURUHASHI, N., YOKAICHIYA, T., TAKAYAMA, K. and YAJIMA, A. Monoamine Oxidase Activity in Rat Organs during Pregnancy. Tohoku J. Exp. Med., 1994, 172 (1), 1-8-To elucidate the involvement of MAO activity in rat pregnancy, female rats exhibiting at least 3 consecutive 4-day estrous cycles were used. The day of sperm-positive smears was designated as day 0 of pregnancy. The rats were sacrificed on day 2 of diestrus (diestrus-2) and days 7, 14 and 21 of gestation. The organs were collected and weighed, and mitochondrial fractions were prepared for MAO analysis with kynuramine dihydrobromide as substrate. Specific activity (pmol/mg of protein/min) in the ovary is significantly lower in rats on diestrus-2 (792±127) (mean±S.D.) than on day 14 (1, 038±140) and day 21 (1, 580±61) of pregnancy. However, the levels of MAO activity in the uterus of rats on diestrus-2 (899±266) is higher than on day 21 (570±80) of pregnancy. The MAO activity in the placenta on day 21(644±82) was higher than day 14 (356±88) of pregnancy. Total MAO activity per organ (nmol/min) of ovary on day 21(27.2±5.2) is higher than on diestrus-2 (8.7±3.0) and on day 14 (14.2±3.6). Our results suggest that MAO activity in rat reproductive organs such as uterus, ovary, and placenta might fluctuate significantly near term, in correlation with steroid levels, tissue catecholamine contents and so on, in order to maintain pregnancy.View full abstractDownload PDF (788K) -
TETSUO SATO, TOMOHARU AKIBA, HIROSHI SUZUKI, MITSURU NAKASATO, SATOSHI ...1994 Volume 172 Issue 1 Pages 9-15
Published: 1994
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSATO, T., AKIBA, T., SUZUKI, H., NAKASATO, M., SATO, S., YOSHIKAWA, M. and YAMAKI, S. Histopathological Findings of Lung Vessels in Five Children with Primary Pulmonary Hypertension. Tohoku J. Exp. Med., 1994, 172 (1), 9-15-A histopathologic study was carried out on pulmonary vessels of lung tissues from 5 children with primary pulmonary hypertension (PPH), in whom diagnosis was made on the basis of cardiac catheterization. Histological sections of lung were obtained in 4 patients by open lung biopsy and in one patient by closed needle necropsy soon after death. In all cases the vascular diseases were classified as grade 6 of the Heath-Edwards. Morphologic studies of small pulmonary arteries revealed medial hypertrophy (41.0%), intimal cellular proliferation (2.5%), intimal fibroelastic proliferation (36.9%) and medial destructive changes (19.6%) such as dilatation lesions, plexiform lesions, arteritis, or fibrinoid necrosis. Index of pulmonary vascular disease (IPVD) was 1.6 to 3.4 (mean 2.5). The advancement of these pulmonary changes sufficiently explains the extremely poor prognosis of children with PPH.View full abstractDownload PDF (2554K) -
MASANORI SUZUKI, TOHRU TAKAHASHI, KIYOAKI OUCHI, SEIKI MATSUNO1994 Volume 172 Issue 1 Pages 17-28
Published: 1994
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSUZUKI, M., TAKAHASHI, T., OUCHI, K. and MATSUNO, S. Perineural Tumor Invasion and Its Relation with the Lymphogenous Spread in Human and Experimental Carcinoma of Bile Duct. A Computer-aided 3-D Reconstruction Study. Tohoku J. Exp. Med., 1994, 172 (1), 17-28-The pathogenesis of perineural tumor invasion was studied by computer aided 3-D reconstruction of bile duct wall from two patients submitted to surgery for hepatohilar carcinoma, in order to analyze how and via what route carcinoma reaches the perineural spaces. Rabbits with VX2 carcinoma implanted in the wall of the common bile duct were also examined. It was found that carcinomas growing along the perineural spaces had abundant connections with the tumors growing outside the nerve, especially those lurking in the lymphatics. In an additional analysis on the wall tissues of bile duct from 35 patients operated for carcinoma, the degree of invasion into perineural spaces proved to correlate with that into lymphatics much higher than with venular invasion. Thus it is likely that tumors reach distant nerves mainly via lymphatics, i.e., forming satellite lymphogenous foci around nerves and then, as a second step, breaking into the perineural spaces.View full abstractDownload PDF (7841K) -
YOSHIAKI KONDO, YUTAKA IGARASHI, KEI KUDO, NOBUYUKI TAKAHASHI, OSAMU I ...1994 Volume 172 Issue 1 Pages 29-38
Published: 1994
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKONDO, Y., IGARASHI, Y., KUDO, K., TAKAHASHI, N., ITO, O., INOUE, C.N., FUJIWARA, I. and ABE, K. Electrophysiological Analysis of Effect of Propranolol in Rabbit S2 Proximal Straight Tubule. Tohoku J. Exp. Med., 1994, 172 (1), 29-38-The effect of dl-propranolol on the basolateral membrane potential (Vb) of in vitro microperfused S2 proximal straight tubules of the rabbit kidney was examined using conventional microelectrode techniques. In the steady-state condition, the average of 23 measurements of Vb was -44.8±2.0mV. Addition of 10-4mol/l of dl-propranolol to the basolateral solution rapidly depolarized Vb by 12.1±1.3mV in 20sec (n=15). The same dose of d-isomer of propranolol, which has no beta-blocking effect, also depolarized Vb to a similar extent. The non-selective beta-blocker nadolol, which possesses no membrane stabilising activity, had no effect on Vb. Depolarization of Vb by dl-propranolol in 20 seconds (propranolol-induced ΔVb) occurred in a dose-dependent manner. In the presence of 1mmol/l Ba++ in basolateral solution, propranolol-induced ΔVb was strongly inhibited. The stilbene derivative DIDS at 1mmol/l did not change propranolol-induced ΔVb, whereas the elimination of Cl- from the ambient conditions increased propranolol-induced ΔVb. The minimization of the luminal Na+-coupled organic solute transporter by collapsing of the lumen did not inhibit propranolol-induced ΔVb, indicating the lack of effect of propranolol on luminal Na+-coupled transporters. Ouabain at 10-3mmol/l in the bath did not eliminate propranolol-induced ΔVb, indicating the presence of a target transporter other than Na+/K+ ATPase for propranolol. These results suggest the following; 1) propranolol has a depolarizing effect on Vb in proximal tubule; 2) the effect of propranolol is independent of Cl- transport or Na+-coupled transporters in the luminal membrane; 3) propranolol depolarizes Vb by inhibiting the K+ channel in the basolateral membrane of S2 proximal tubule.View full abstractDownload PDF (958K) -
TORU SATO1994 Volume 172 Issue 1 Pages 39-49
Published: 1994
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSATO, T. Changes in the Gap Junction of Rat Exocrine Pancreatic Cells Induced by Long-term Ethanol Ingestion. Tohoku J. Exp. Med., 1994, 172 (1), 39-49-Fourteen Male Wistar rats were orally administered 20% ethanol ad libitum in place of water. After 1, 2, 3, 6 and 12 months of ethanol ingestion, the gap junctions of pancreatic acinar and ductal cells were observed by transparent electron microscopy. The result was compared with fifteen control animals. In the control, the ductal cells proved to have only small sized (0.1 micron in length) gap junction located in the apical domain as a part of junctional complex. While, in the acinar cells, not only small but large-sized gap junctions several microns in length were observed in the baso-lateral domain. After 3 months of ethanol ingestion, the gap of these two types of gap junctions was replaced with a continuous, granular structure. In addition, irregularly running gap junctions were observed after 6 months of ethanol ingestion. The functional implication of these morphological changes is not clear.View full abstractDownload PDF (5346K) -
KOZO ITO1994 Volume 172 Issue 1 Pages 51-57
Published: 1994
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSITO, K. Histological Evaluation of Mixed Mesodermal Tumor of the Ovary. Tohoku J. Exp. Med., 1994, 172 (1), 51-57-Mixed mesodermal tumor (MMT) arising from the ovary is very rare, but we recently encountered two cases of this tumor. The tumors were examined histologically using hematoxylin-eosin, alcian blue, periodic acid-Schiff (PAS), and toluidine blue staining, as well as immunohistochemical staining for S-100 protein. The two patients were postmenopausal women aged 68 and 58 years, respectively. The carcinomatous region of the mixed tumor was endometrioid carcinoma in Patient 1 and serous cystadenocarcinoma in Patient 2, while the sarcomatous region was chondrosarcoma in both patients. The chondrosarcoma tissue was positive for PAS, alcian blue, toluidine blue, and S-100 protein.View full abstractDownload PDF (6263K) -
Effective Treatment of L-CarnitineAKIRA ISHIDA, ATSUKO GOTO, YASUSHI TAKAHASHI, WAKO NAKAJIMA, HIROKAZU ...1994 Volume 172 Issue 1 Pages 59-64
Published: 1994
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSISHIDA, A., GOTO, A., TAKAHASHI, Y., NAKAJIMA, W., ARAI, H., TAZAWA, Y. and TAKADA, G. A Preterm Infant with Secondary Carnitine Deficiency due to MCT Formula-Effective Treatment of L-Carnitine-. Tohoku J. Exp. Med., 1994, 172 (1), 59-64-We report a preterm infant who was prescribed an MCT formula and subsequently developed carnitine deficiency with liver dysfunction and an elevation of serum CK level. A male infant who had been born at 24 weeks' gestation with birth weight 799g, was fed with an MCT formula containing 76.8% of all kinds of lipids, because of his steatorrhea after the 30th day. On the 100th day, he was noted hepatomegaly and elevation of serum levels of AST, ALT and CK. The needle biopsy of the liver indicated the existence of the liver damage. He showed low serum carnitine with high urinary loss of acylcarnitine and dicarboxylic aciduria. Administration of L-carnitine was an effective treatment. The carnitine deficiency might be exaggerated by an increased urinary loss of acylcarnitine. We should be cautious of the risk of carnitine deficiency in preterm infants during prolonged use of MCT formula.View full abstractDownload PDF (538K) -
HIRONOBU IKEHATA1994 Volume 172 Issue 1 Pages 65-81
Published: 1994
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSIKEHATA, H. Comparison of DNA Polymerases α, δ, and ε of Mouse Cell Line FM3A and Its Temperature-Sensitive Mutant tsFT20. Tohoku J. Exp. Med., 1994, 172 (1), 65-81-DNA polymerases (pol) α, δ and ε of a mouse cell line FM3A and its temperature-sensitive derivative tsFT20, which is defective in DNA replication at a non-permissive temperature, were purified by chromatographic procedures monitored by a set of relatively specific assays for the respective DNA polymerase activities. The pol ε activity was separated into two fractions with similar enzymatic properties except for their optimal KCl concentrations and processivities. The fractions of pol δ and ε were not homogeneous, but their identities were confirmed by their sensitivities to DNA polymerase inhibitors, their associated 3'→5' exonuclease activities, optimal concentrations of salts, dependencies on the proliferating cell nuclear antigen and processivities in polymerization, which also excluded significant contamination with other DNA polymerases. Of the DNA polymerases prepared from tsFT2O cells, only pol α showed greatly decreased activity and remarkable sensitivity to the non-permissive temperature, demonstrating that pol δ and ε, the other polymerases supposed to be involved in nuclear DNA replication, are unequivocally different entities from poi α. The level of pol ε activity tsFT20 was also significantly lower than in the parental cells, suggesting cooperation and/or interaction between pol α and ε, and some relevance of pol ε to DNA replication.View full abstractDownload PDF (2900K) -
ATSUSHI KANNO, HITOSHI AMAKASU, WAKIO TORINUKI, TAKESHI YAMAMOTO, MOTO ...1994 Volume 172 Issue 1 Pages 83-90
Published: 1994
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKANNO, A., AMAKASU, H., TORINUKI, W., YAMAMOTO, T., ISHII, M., NUMATA, N. and OHORI, H. Two Cases of Porphyria Cutanea Tarda Associated with Chronic Hepatitis Positive for an Antibody against Hepatitis C Virus. Tohoku J. Exp. Med., 1994, 172 (1), 83-90-We report two cases of porphyria cutanea tardy (PCT) positive for the antibody against hepatitis C virus (anti-HCV). The serological and histological examinations revealed that they were persistently infected with HCV and were suffering from liver disease compatible with chronic viral hepatitis. It is suggested that one of the factors which contribute to liver damage of patients with PCT may be HCV infection. It now may be advisable to examine anti-HCV in PCT patients with liver disease.View full abstractDownload PDF (6548K) -
TOKUTARO SATO, MASAO HIWATARI, ETSURO SATO, HIROSHI WATANABE, MINORU Y ...1994 Volume 172 Issue 1 Pages 91-96
Published: 1994
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSATO, T., HIWATARI, M., SATO, E., WATANABE, H. and YASUJIMA, M. Changes of Plasma Lipid and Lipoproteins by Rehabilitation Therapy for Stroke. Tohoku J. Exp. Med., 1994, 172 (1), 91-96-Changes of the plasma levels of lipi d by rehabilitation therapy for approximately 10 weeks were studied in 14 cases of stroke. Hypercholesterolemia or hypertriglyceridemia was present in a half cases of stroke, and HDL cholesterol was lower than the normal level in most cases before starting rehabilitation therapy. After rehabilitation therapy, hypertriglyceridemia was normalized in most cases. On the other hand, the plasma levels of cholesterol and LDL cholesterol were decreased slightly. HDL cholesterol was increased and atherogenic index was improved by rehabilitation therapy.View full abstractDownload PDF (589K)
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