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Volume 170 (1993)
- Issue 4 Pages 207-
- Issue 3 Pages 135-
- Issue 2 Pages 63-
- Issue 1 Pages 1-
Volume 170, Issue 2
Displaying 1-8 of 8 articles from this issue
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YUKO SHINTAKU, TOSHIFUMI TAKABAYASHI, HIROYUKI SASAKI, NOBUYOSHI OZAWA ...1993 Volume 170 Issue 2 Pages 63-69
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSHINTAKU, Y., TAKABAYASHI, T., SASAKI, H., OZAWA, N. and YAJIMA, A. Sister Chramatid Exchanges in Mouse after Exposure to Pulse-Wave Ultrasound in Utero. Tohoku J. Exp. Med., 1993, 170 (2), 63-69 - The induction of sister chromatid exchanges (SCEs) was investigated in mice after a ten in exposure, in vivo, to 2MHz focused, pulse-wave ultrasound with a pulse repetition rate of 1000 Hz, pulse duration of 10μsec. The bone marrow cells of the pregnant female mice and the fetal liver cells were analyzed. The cell cycle specific metaphase patterns were additionally evaluated. In the bone marrow cells, the mean frequencies of SCEs were 2.77 in control, 3.56 in the cells exposed to ultrasound at 586.2 mW/cm2 (spatial average temporal average, SATA); in the fetal liver cells, 2.64 in control, 3.84 in the cells exposed. The frequencies of SCEs significantly were increased by the treatment. Faster cell kinetics was observed in fetal liver cells than bone marrow cells of pregnant female. But there was no cell-growth inhibitory effect of ultrasound on both bone marrow and fetal liver cells. In fetal liver cells, the critical acoustic power was 160.0-278.9mW/cm2 (SATA), - sister chromatid exchange; ultrasound; in vivoView full abstractDownload PDF (581K) -
MOTOHIKO OKANO, TAKAO AYA, FUMIO MIZUNO, KENZO TAKADA, TOYORO OSATO1993 Volume 170 Issue 2 Pages 71-79
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOKANO, M., AYA, T., MIZUNO, F., TAKADA, K. and OSATO, T. High Epstein- Barr Virus (EBV) Susceptibility of Both Lymphoblastoid and Lymphoma Cell Lines Derived From a Japanese Patient with EBV Genome-Positive Burkitt's Lymphoma. Tohoku J. Exp. Med., 1993, 170 (2), 71-79 - A Lymphoma cell line from the tumor tissue was established spotaneously from a Japanese patient with Epstein-Barr virus (EBV) genome-positive Burkitt's lymphoma (BL). Additionally lymphoblastoid cell lines from peripheral blood of this patient were established either spontaneously or by in vitro infection with B95-8 EBV. Lymphoma cells showed monoclonal surface immunoglobulins (kappa and gamma) with specific chromosomal translocations, t (8; 14). In contrast, lymphoblastoid cells expressed polyclonal surface immunoglobulins without specific chromosomal abnormalities. Lymphoma cells made colonies in soft agarose approximately 10 times more than those of the lymphoblastoid cells. When each cell line was cultured at lower temperature of 33°C, treated with 12-O-tetradecanoyl-phorbol- 13-acetate (TPA), and superinfected with P3HR-1 EBV, all cell lines expressed 5 to 10 times higher levels of EBV early antigens (EA) and viral capsid antigen (VCA) than lymphoblastoid cell lines from healthy controls. Furthermore, lymphoblastoid cell lines obtained from peripheral blood of this patient during the period of remission also exhibited high EA and VCA inducibility and superin- fectibility. These findings suggested that the lymphoid cells in this patient were genetically highly susceptible to EBV infection, and this evidence possibly linked to the lymphomagenesis of EBV genome-positive BL. - Epstein-Barr virus; inducibility; superinfectibility; Burkitt's lymphomaView full abstractDownload PDF (890K) -
MANABU KAGAYAMA, YASUYUKI SASANO, HIROTOSHI AKITA1993 Volume 170 Issue 2 Pages 81-91
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKAGAYAMA, M., SASANO, Y. and AKITA, H. Lectin Binding in Bone Matrix of Adult Rats with Special Reference to Cement Lines. Tohoku J. Exp. Med., 1993, 170 (2) 81-91 - The present study was designed to localize glycocon- jugates recognized by four lectins (LFA, MPA, WGA, and ConA) in adult rat bone, histochemically and biochemically. LFA, a lectin recognizing sialic acid, stained intensely the cement lines of calvariae and the interface between cartilage and bone of mandibular condyles, which were also stained with WGA, and intermittently stained with ConA. The irregular bone surface of calvariae displayed intense positive reaction with LFA and WGA, while the osteoid of mandibular condyles and the smooth bone surface of calvariae were intensely stained with WGA and ConA, but weakly stained with LFA and MPA. MPA recongnized osteoclasts. At the superficial layer of mandibular condyles, the bone matrix appeared positive as granules with LFA and as patches with MPA, WGA and ConA. Lectin blotting analysis of bone extracts revealed two LFA positive bands at molecular weights of 18kDa and 45kDa. The former was positive with WGA, ConA, and MPA, while the latter was positive with MPA. The result suggests that the LFA-binding glycoconjugates, possibly 18kDa and/or 45kDa sialoglycocon- jugates, may contribute to cementing mechanism of cement lines and/or to the bone formation in remodeling process as well as in intramembranous and intracartilaginous osteogenesis. - lectin binding; cement line; histochemistry; lectin blotting; bone extractsView full abstractDownload PDF (2924K) -
YASUHIKO OTAKE, MASARU MIYAO, SHIN'YA ISHIHARA, MINAKO KASHIWAMATA, TA ...1993 Volume 170 Issue 2 Pages 93-102
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOTAKE, Y., MIYAO, M., ISHIHARA, S., KASHIWAMATA, M., KONDO, T., SAKAKIBARA, H. and YAMADA, S. An Experimental Study on the Objective Measurement of Accommodative Amplitude under Binocular and Natural Viewing Conditions. Tohoku J. Exp. Med., 1993, 170 (2), 93-102 - A high resolution optometey makes it possible to measure changes in refractive power objectively using reflected light from the fundus of the eye. There are limitations in that the subject has to see the target monocularly through a hole in the instrument. In the present study, we attempted to measure the accommodation and pupillary response with a newly developed device as a means to measure these factors in both binocular and natural viewing conditions. We compared the subjective accommodative amplitude levels obtained from the near-point distance with those of objective accommodative amplitude obtained in earlier studies using a monocular internal target. The amplitude of accommodation of the cases with binocular, natural viewing conditions was approximately 0.7 D larger than with monocular, internal target gazing. In previous studies, the difference between the subjective amplitude of accommodation and the results of the objective measurements, had been reported to be approximately 2 D, and was explained by the depth of focus. However, the difference between the subjective and objective amplitude of accommodation under binocular, natural viewing was smaller than that between the subjective and objective measurements (under monocular internal target gazing) from previous studies. This leads us to believe that besides the depth of the focus, we should consider the fact that the advantageous conditions inherent in binocular, natural viewing may increase the amplitude of accommodation. In the measurement of accommodative amplitude, the use of objective measuring methods are not affected by the subjects' subjective judgement. - accommodation; aging; binocular vision; monocular vision; optometryView full abstractDownload PDF (915K) -
TEN-JEN LIAO, HIROMOTO NAKANISHI, HIROYASU NISHIKAWA1993 Volume 170 Issue 2 Pages 103-112
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSLIAO, T.-J., NAKANISHI, H. and NISHIKAWA, H. The Effect of Acupuncture Stimulation on the Middle Latency Auditory Evoked Potential. Tohoku J. Exp. Med., 1993, 170 (2), 103-112 - The effects of acupuncture stimulation (AS) on the middle latency auditory evoked potentials (MLAEPs) were studied in 19 normal male volunteers. Scalp recordings were made from 21 points (including Cz) and posterior auricular muscle (PAM) electrodes referenced to the linked mastoid. Four components of MLAEPs (Po, Na, Pa and Nb) and two components of PAM reflex (N12 and P17) were statistically analyzed for changes in latency and amplitude. Wave phase of Po and Na reversed to N12 and P17, respectively, and the peak-to-peak amplitude of Po-Na, Na-Pa, Pa-Nb and N12-P17 showed a marked increase during AS to the ipsilateral side whereas no change in latency was detected. Each component's isovoltage topographic maps and dipole locations were calculated to be near both PAM (Po and Na) and temporal positions (Pa and Nb) during AS. These data showed that AS can promote MLAEPs activity and these signals (Po and Na) may originate from increased activity in N12 and P17 of the PAM reflex, respectively, and that the auditory cortex of the temporal gyrus is the generator substrate of Pa and Nb. - middle latency auditory evoked potential; acupuncture stimulation; posterior auricular reflex; human subjectsView full abstractDownload PDF (873K) -
CESAR G. PAEZ, NOBUO YAEGASHI, SHINJI SATO, AKIRA YAJIMA1993 Volume 170 Issue 2 Pages 113-121
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSPAEZ, C.G., YAEGASHI, N., SATO, S. and YAJIMA, A. Prevalence of Serum IgG Antibodies for the E7 and L2 Proteins of Human Papillomavirus Type 16 in Cervical Cancer Patients and Controls. Tohoku J. Exp. Med., 1993, 170 (2), 113- 121 - Relationship between the prevalence of the antibodies to HPV16E7 and L2 proteins and the development of cervical cancer was examined. Sera from 57 patients with invasive cervical cancer and from 200 age-matched healthy blood donors (16 to 64 years old) were examined for antibodies against E7 and L2 proteins of human papillomavirus (HPV) type 16. Bacterially expressed fusion antigens were used in a Western immunoblot assay. Ten (18%) of the patients and 10 (5%) of the controls were positive for only E7. Each eighteen of the patients (32%) and of the controls (9%) were positive for only L2. Three of the patients and 2 of the controls were positive for both E7 and L2 antibody reactivity. The patients' prevalence of antibodies for the E7 and L2 proteins was significantly higher than that of the controls (E7: χ2=14.3, p<0.01; L2: χ2=23.8, p<0.01). On the other hand, neither sex specific difference in the antibody prevalence was observed, nor was there any difference in the antibody prevalence with age. Our findings indicate that antibodies to the HPV16L2 proteins could be a parameter for- cervical cancer development as well as those to the HPV16E7 proteins. human papillomavirus; type 16; serum antibody; L2 protein; E7 proteinView full abstractDownload PDF (1257K) -
KEI NUMAZAKI, SHUNZO CHIBA, YUUICHI NIIDA, MAYUMI KOMATSU, NOBUO HASHI ...1993 Volume 170 Issue 2 Pages 123-129
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNUMAZAKI, K., CHIBA, S., NIIDA, Y., KOMATSTT, M. and HASHIMOTO, N. Evaluation of Diagnostic Assays for Neonatal and Infantile Chlamydial Infections. Tohoku J. Exp. Med., 1993, 170 (2), 123-129 - Several diagnostic assays for neonatal and infantile chlamydial infections, isolation with tissue culture, antigen detection by enzyme immunoassay (ETA) (IDEIA Chlamydia test), a nonisotopic DNA probe (Gen-Probe PACE 2 assay), serum IgM antibody detection by ETA (SeroELISA Chlamydia TRUE IgM), and polymerase chain reaction (PCR), were evaluated. Of 210 clinical specimens (170 nasopharyngeal and 40 conjunctival swabs) from 53 neonates and 102 infants with respiratory insufficiency and respiratory tract infections which were suspected to be associated chlamydial infection, chlamydial antigens were detected in 30 by IDEIA Chlamydia. Of these 3Q samples, C. trachomatis was isolated from 27 specimens. Samples from 15 neonates and 6 infants were culture-positive and IDEIA Chlamydia-positive. Of 30 samples, 27 were tested with PCR and 8 with DNA probe. Twenty-three of 27 specimens were positive with PCR, while only one specimen was positive with DNA probe. ETA can be used for the diagnosis and screening of neonatal and infantile chlamydial infections. - Chlamydia trachomatis; ETA; pneumoniaView full abstractDownload PDF (696K) -
ICHIRO NAKAZAWA, MASAMOTO IWAIZUMI, KAZUO OHUCHI1993 Volume 170 Issue 2 Pages 131-133
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNAKAZAWA, I., IWAIZUMI, M. and OHUCHI, K. Some Features in Prostaglandin Synthesis of the Cancer Cells Which Metastasized into Liver from Intestinal Cancer Lesion. Tohoku J. Exp. Med., 1993, 170 (2), 131-133 - In order to study the mechanism of cancer metastasis, AH100B cells, an ascitic hepatoma cell line, were transplanted into the small intestine of male Donryu rats. Each metastatic nodule in the liver was collected with the respective intestinal lesion. Each sample thus obtained was injected into the peritoneal cavity of male Donryu rats to make free cancer cells. Then, the cancer cells, having an intact cell surface, of the metastatic and primary intestinal lesion were collected respectively. After washing in Dolbecco's PBS (Ca2+ and Mg2+-free, pH 7.2), the definite numbers of cancer cells of the metastatic and primary intestinal lesion were incubated in the PBS containing [1-14C]-AA at 25°C for 30min, respectively. AA metabolites formed during the incubation period were extracted and subjected to TLC, followed by autoradiography. Each radioactive part was scraped off the plate and measured for its radioactivity. The pattern of the ability to synthesize PGs was different between the cancer cells which metastasized to the liver and those of the primary lesion, that is, percentage values of PGE2 and PGF2α were higher (p< 0.01) in the cancer cells which metastasized to liver as compared with those of the primary intestinal lesion. These results suggest that PGs produced by hepatic metastatic cancer cells might play an important role in cancer metastasis.- cancer metastasis; prostaglandin; liver; intestinal cancerView full abstractDownload PDF (377K)
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