The feto-maternal transportation of substances through the villi of human placentae from gravidae of 2 through 10 months of pregnancy and human placenta charged with fat
in vitro was electron-microscopically studied.
The following conclusions in summary were arrived at:
1) Promontory-like cytoplasmic protrusion apparently partaking in the processes of feto-maternal substance transportation through and secretory function of the placenta, and many microvilli related with pinocytosis were found on the free surface of the layer of syncytiotrophoblasts. The endoplasmic reticula gradually changed into route of substance transportation and the surface of basement membrane of syncytiotrophoblasts and cytotrophoblasts became more and more conspicuously marked by basal infoldings and microvilli-like protrusion, with the advance of months of pregnancy.
2) The basement membrane is usually a homogenous structure formed by aggregation of fine granulous substance and was found to grow thicker with fetal age. It showed soundly morphological changes and included some matter indicative of substance transportation through it.
3) The stromal collagenous fibers increased both in number and in size with progress of pregnancy and were found to form a part of fluid route through the stroma.
4) The fetal capillaries had a basement membrane, their lumen enlarged and their endothelium thined from month to month. Here, the main substance transportation was apparently effected through pinocytosis.
5) Findings were obtained that fat particles are taken in by syncytiotrophoblasts from through pinocytosis. A part of the fat particles pass into cytotrophoblasts from syncytiotrophoblasts. The fat particles reaching the basement membrane beneath the chorionic epithelium permeate the membrane in diffuse form, but a part of them were seen going through the membrane in unchanged particle shape.
6) The fat transportation in stroma was found mainly passing through the cytoplasm of stroma cells from the chorionic villus side to the fetal capillary side. Besides, some fat particles were found extracellularly. Fat taken up by the perivasei lar pericytes was observed slowly passing over into the endothelial cells of the fetal-side capillaries, and through the cells finally to flow into the capillary blood through ruptures in the cell membrane.
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