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Volume 137 (1982)
- Issue 4 Pages 349-
- Issue 3 Pages 233-
- Issue 2 Pages 115-
- Issue 1 Pages 1-
Volume 137, Issue 4
Displaying 1-16 of 16 articles from this issue
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HIROSHI HOSHIAI, SHIGEKI UEHARA, MASAKUNI SUZUKI, CLAUDE A. VILLEE1982 Volume 137 Issue 4 Pages 349-360
Published: 1982
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSHOSHIAI, H., UEHARA, S., SUZUKI, M. and VILLEE, C.A. The Variations of Estrogen Receptor, Progesterone Receptor, cAMP, ODC Activity and RNA Synthesis of Deciduomata in Pseudopregnant Rats. Tohoku J. exp. Med., 1982, 137 (4), 349-360-The present study was done to define the conditions of implantation of fertilized ovum from serial day-to-day changes in the tissue weight of deciduomata in pseudopregnancy, estrogen receptor (ER), progesterone receptor (PR), cAMP content, RNA synthesizing capacity and ornithine decarboxylase activity (ODC). Pseudopregnancy was induced in Wistar rats in the estrus morning by vaginocervical stimulation. At the 4th day of pseudopregnancy deciduomata was induced by intraluminal instillation of olive oil with simultaneous oophorectomy and intramuscular injection of 20mg progesterone depot (the day of induction of decidualization was arbitrarily expressed as D0 and the ensuing days, as D1, D2 and so on). The number of binding sites of ER and PR was estimated by exchange assay using dextran charcoal. cAMP content and RNA synthesizing capacity were determined by RIA and 3H-UTP uptake, respectively. ODC activity was measured by the method of Jänne and Williams-Ashman. Following the induction of deciduomata the uterus weight (unilateral) increased abruptly to 2-fold at D2 and approximately 30-fold (4.5g) at D8 and declined thereafter. ER diminished in a linear fashion from 2.77×10-10M at D2 to 0.53×10-10M at D7. On the other hand, PR was estimated 2.9×10-10 at D2, 2.5×10-10M at D5 and decreased further at D6 and D7. cAMP was 77nmoles/mg protein at D2 and decreased rapidly from D3 onward. RNA synthesizing capacity declined in a linear fashion from D3 to 1/2 at D5 and 1/3 at D7. ODC was found 1.7 CO2 nmoles/mg protein at D2, decreased at D3 and D4, but remained nearly the same at D4 and D5. -deciduomata; ODC activity; pseudopregnancy; hormone receptor; ratsView full abstractDownload PDF (1114K) -
KATSUNORI OGURO, HISAKUNI HASHIMOTO, MITSUYOSHI NAKASHIMA1982 Volume 137 Issue 4 Pages 361-368
Published: 1982
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOGURO, K., HASHIMOTO, H. and NAKASHIMA, M. Effects of Thymoxamine on Ouabain-Induced Arrhythmias in Dogs. Tohoku J. exp. Med., 1982, 137 (4), 361-368 - The effect of thymoxamine, a pure alpha-adrenoceptor blocking agent, on ouabain-induced arrhythmias was investigated in dogs to clarify whether thymoxamine has an arrhythmogenic or an anti-arrhythmic action. Thymoxamine, 1mg/kg i.v., inhibited the manifestation of ventricular arrhythmias (VA) and the pressor effect produced by ouabain infusion (1μg/kg/min i.v.) as 5mg/ kg i.v. of phentolamine did. Low doses of these drugs did not affect the VA. Simultaneous infusion of phenylephrine (5μg/kg/min i.v.) with ouabain enhanced the manifestation of VA accompanying pressor effects, which were inhibited by thymoxamine (1mg/kg) in a parallel manner. Infusion of isoprenaline (0.2μg/ kg/min i.v.) with ouabain enhanced the manifestation of VA, but the enhancement was to a lesser extent than with phenylephrine. Thymoxamine, 1mg/kg, did not affect the VA. The infusion of CaCl2 (20mg/kg/min i.v.) with ouabain enhanced the manifestation of VA, which was not inhibited by thymoxamine. These results show the possibility that the protective effect of thymoxamine on the manifestation of VA may be mainly associated with inhibitions of pressor effects mediated through vascular alpha-adrenoceptors. - alpha blocker; thymoxamine; phentolamine; ouabain-induced arrhythmiasView full abstractDownload PDF (712K) -
FUMIO UMEDA, KEN-ICHI KATO, HIROSHI IBAYASHI, MASAHARU KOJIMA, YOZABUR ...1982 Volume 137 Issue 4 Pages 369-377
Published: 1982
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSUMEDA, F., KATO, K., IBAYASHI, H., KOJIMA, M., SHIBATA, Y. and YAMAMOTO, T. Inhibitory Effect of Vitamin E on Lipoperoxide Formation in Rat Adrenal Gland. Tohoku J. exp. Med., 1982, 137 (4), 369-377 - The tissue uptake of vitamin E administered to the rats was strikingly high in the adrenal glands, and microradioautographic studies showed that the vitamin was localized morphologically in the cells of fascicular and reticular zones of the adrenal cortex, where lipofuscin pigment appears with aging. On the other hand, it was confirmed that the TBA content in rat plasma and adrenal gland was elevated following whole-body 60Co irradiation as an aging model. It was also demonstrated that the TBA content in rat adrenal markedly increased in conditions of vitamin E deficiency with and without irradiation. - vitamin E; lipoperoxide formation; rat adrenal glandView full abstractDownload PDF (3270K) -
MASATOSHI OHTAKE, GORO TAKADA, SHIGEAKI MIYABAYASHI, NOBUHIRO ARAI, KE ...1982 Volume 137 Issue 4 Pages 379-386
Published: 1982
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOHTAKE, M., TAKADA, G., MIYABAYASHI, S., ARAI, N., TADA, K. and MORINAGA, S. Pyruvate Decarboxylase Deficiency in a Patient with Leigh's Encephalomyelopathy. Tohoku J. exp. Med., 1982, 137 (4), 379-386 - A 23-month-old boy with progressive muscular hypotonia and mental and motor deterioration was described. Four days after the admission he had a respiratory arrest and required the care in a respirator thereafter. Laboratory examinations showed metabolic acidosis and high levels of pyruvate, lactate and alanine in blood and cerebrospinal fluid. Oral administration of thiamine-HCl and lipoic acid was noneffective and he died 7 months after the admission. Autopsy findings were compatible with subacute necrotizing encephalomyelopathy (Leigh's disease). The activity of pyruvate decarboxylase in autopsy samples was not detectable and that in fibroblasts was 9% of that in control cell lines. The present study confirmed that pyruvate decarboxylase deficiency is one of the causes of Leigh's disease. - pyruvate decarboxylase deficiency; Leigh's diseasecView full abstractDownload PDF (1427K) -
TAKESHI YOSHIDA, HIDETOSHI SASAKI, KIICHIRO NODA, SUNAO YACHIKU, SHIGE ...1982 Volume 137 Issue 4 Pages 387-390
Published: 1982
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSYOSHIDA, T., SASAKI, H., NODA, K., YACHIKU, S. and KANEKO, S. Neuro-Pharmacologic Studies on Neurogenic Bladder after Radical Hysterectomy. Tohoku J. exp. Med., 1982, 137 (4), 387-390 - Miction function of patients who had had radical hysterectomy was investigated neuro-pharmacologically. The patients were divided into two groups according to the operation, with or without preservation of the plexus pelvicus, and bladder and urethral functions were compared by the bethanecol test and phentolamine test. The group with transected plexus pelvicus showed neurogenic bladder with impairment of functions of parasympathetic and sympathetic nerves and the preserved group showed much less impairment in functions of both nerves. - neurogenic bladder; radical hysterectomy; neuro-pharmacologic studyView full abstractDownload PDF (374K) -
TAMOTSU YOSHIOKA, YOSHIKI TAKEHARA, MIE SHIMATANI, KOUICHI ABE, KOZO U ...1982 Volume 137 Issue 4 Pages 391-400
Published: 1982
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSYOSHIOKA, T., TAKEHARA, Y., SHIMATANI, M., ABE, K. and UTSUMI, K. Lipid Peroxidation and Antioxidants in Rat Liver during Development. Tohoku J. exp. Med., 1982, 137 (4), 391-400 - The concentration of lipid peroxidation was extensively high in rat fetuses and early newborns. However, it declined sharply thereafter. Superoxide dismutase (SOD) activity was approximately 10% of the adult level during 5 days postpartum. The enzyme activity began to increase after the 10th day to 60% of the adult level at the 20th day. Catalase activity was low in the fetal period, corresponding to approximately 20% of the adult level, but increased rapidly after birth reaching approximately 5000 of the adult level at 5-7 days postpartum. Glutathione peroxidase (GSH-Px) activity was measured to amount to only 7% of the adult level in the fetal and early newborn period. The level of this activity was approximately 20% of the adult level at the 20th day. The difference in GSH-Px activity became wide between sexes after the first 30 days of life; the male adult level was 61% of the female adult level. The concentration of vitamin E was low in the fetus. It increased by a factor of 10 times within a few days after birth, and thereafter it decreased gradually. Fetal and early newborn livers have low enzymatic defense capabilities against possible deleterious effects of lipid peroxidation processes. lipid peroxidation; superoxide dismutase; glutathione peroxidase; catalase; vitamin EView full abstractDownload PDF (938K) -
TAKESI SAITO, FUMIYA OKI1982 Volume 137 Issue 4 Pages 401-408
Published: 1982
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSAITO, T, and OKI, F. The Strain-Energy Density Function of the Urinary Bladder. Tohoku J. exp. Med., 1982, 137 (4), 401-408 - In previous articles we proposed a finite deformation theory of the urinary bladder. The present paper is the third article in a series of these works. Our aim is to study more carefully the strain-energy density function W of the urinary bladder, because the determination of this function W is one of the central problems in the finite deformation theory of a hyperelastic continuum. We found that, except for an infinitesimally small deformation region where Hook's law is valid, the third term in W of the Valanis-Landel type takes much smaller values than the other two terms, so that this term can be neglected. Hence, the formula giving the true stress t1 for the uniaxial extension test is reduced to the same one as the true stress t for the equal-biaxial extension test. This may be useful in practical applications. - neurogenic bladder; urodynamics; theory of elastic body; cystometrogramView full abstractDownload PDF (490K) -
SYO ISOGAI, KAZUYOSHI KIKUCHI, MASAAKI KAMEYAMA, YASUO OSHIMA, TSUTOMU ...1982 Volume 137 Issue 4 Pages 409-413
Published: 1982
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSISOGAI, S., KIKUCHI, K., KAMEYAMA, M., OSHIMA, Y., URAYAMA, T. and KOMOTO, M. Plasma Soluble Fibrin Monomer Complexes in the Development of Diabetic Retinopathy. Tohoku J. exp. Med., 1982, 137 (4), 409-413 -To elucidate the relationship between plasma soluble fibrin monomer complexes (SFMC) and retinal or vitreous hemorrhages, 11 diabetics were studied for 15 months. The levels of SFMC in 3 out of the 11 cases were elevated prior to hemorrhages and in the other 8 cases SFMC levels were elevated after hemorrhages. With regard to fibrinogen (Fbg) concentrations, there was no significant difference between the values before and after hemorrhages. The plasma glucose levels did not change significantly during the observation. It was concluded that the SFMC level was not only an indicator of the intracapillary generation of thrombin but an important factor affecting the development of diabetic retinopathy.-diabetic retinopathy; retinal hemorrhage; soluble fibrin monomer complexes;fibrinocoagulopathyView full abstractDownload PDF (454K) -
MINEO IWASA, TSUYOSHI YOKOI, KAORU SAGISAKA1982 Volume 137 Issue 4 Pages 415-421
Published: 1982
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSIWASA, M., YOKOI, T. and SAGISAKA, K. An Interaction between Human Seminal Acid Phosphatase and Canavalia gladiata DC Lectin. Tohoku J. exp. Med., 1982, 137 (4), 415-421 - Interactions between human seminal acid phosphatase (AcP) and five kinds of lectins were studied. Seminal plasma was mixed with the lectins at various ratios. The mixtures were centrifuged and the supernatants were assayed for AcP activity. The activity was effectively reduced only with Canavalia gladiata DC (CG) lectin. CG lectin purified by Sephadex G-200 gel filtration was added to two fractions of AcP separated by Sephadex G-200 gel filtration. There was no difference in the rate of reduction of the two fractions. To isolate seminal AcP by affinity chromatography, CG lectin was coupled to agarose gel. AcP bound with the agarose gel could be eluted with α-methyl-D-mannoside. Specific activity of the purified AcP was elevated to as much as 54-fold. - lectin; seminal plasma; acid phosphataseView full abstractDownload PDF (803K) -
HIROYOSHI ONODERA, TORU HIRATA, HIRAKU SUGAWARA, KOJI SUGAI, BINKO YOD ...1982 Volume 137 Issue 4 Pages 423-428
Published: 1982
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSONODERA, H., HIRATA, T., SUGAWARA, H., SUGAI, K., YODA, B., TOYOTA, T. and GOTO, Y. Platelet Sensitivity to Adenosine Diphosphate and to Prostacyclin in Diabetic Patients. Tohoku J. exp. Med., 1982, 137 (4), 423-428 - We studied the platelet sensitivity to adenosine diphosphate (ADP) and to prostacyclin (PGI2) in order to clarify the mechanism of the onset or development of diabetic retinopathy. The platelet sensitivity to ADP in diabetics with simple and proliferative retinopathy was significantly increased as compared with that in diabetics without retinopathy and in controls. The platelet sensitivity to PGI2 in diabetics with simple and proliferative retinopathy was decreased significantly as compared with the normal value. There was a significant negative correlation between the platelet sensitivity to ADP and that to PGI2 in all diabetics. Since the effect of PGI2 on platelets is to inhibit its aggregation, the decreased sensitivity to PGI2 implies a tendency to accelerate platelet aggregation. The increased platelet aggregation due to the alteration of the platelet sensitivity is considered as an important factor in diabetic microangiopathy. - diabetes mellitus; diabetic retinopathy; platelet sensitivity; prostacyclin (PGI2); adenosine diphosphate (ADP)View full abstractDownload PDF (526K) -
ATSUSHI MURAI, TADAO MIYAHARA, TOMOJI TANAKA, MASAKUNI KAMEYAMA1982 Volume 137 Issue 4 Pages 429-435
Published: 1982
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSMURAI, A., MIYAHARA, T., TANAKA, T. and KAMEYAMA, M. A Simple Method for Quantitative Determination of High Density Lipoprotein 2- and 3-Cholesterol Concentrations by Means of Polyacrylamide Gel Electrophoresis. Tohoku J. exp. Med., 1982, 137 (4), 429-435 - A simple and rapid electrophoretic method was developed to determine quantitatively the cholesterol concentration in two main subfractions of high density lipoprotein, HDL2 and HDL3. A polyacrylamide gel on which prestained serum was fractionated was directly subjected to densitometry for cholesterol concentration. The result, in conjunction with the HDL-cholesterol concentration simultaneously determined by the heparin-Ca2+ method, enabled us to calculate the HDL2- and HDL3-cholesterol concentrations. These values correlated well with those determined by the ultracentrifugal method. Hence, the present method was considered useful for routine clinical application and to be superior to the ultracentrifugal method in subjects with a high level of Lp(a) lipoprotein. - HDL subfraction; Lp(a) lipoprotein; polyacrylamide gel electrophoresis; densitometry; heparin-Ca2+ precipitationView full abstractDownload PDF (965K) -
AKIRA OHNEDA, TAKASHI KOBAYASHI, JIRO NIHEI1982 Volume 137 Issue 4 Pages 437-444
Published: 1982
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOHNEDA, A., KOBAYASHI, T. and NIHEI, J. Evaluation of New Criteria for Diagnosis of Diabetes Mellitus Based on Follow-Up Study of Borderline Diabetes. Tohoku J. exp. Med., 1982, 137 (4), 437-444 - In order to evaluate the validity of recently proposed criteria for diagnosis of diabetes mellitus, a retrospective study was performed in 315 patients with borderline diabetes, who were selected from approximately 4, 000 outpatients under treatment for more than three years. They were divided into two groups, BI and BII, according to the 2-hr blood glucose level in the 50g glucose tolerance test (GTT) : BI between 100 and 135mg/100ml and BII between 135 and 195mg/100ml. In group BI, glucose tolerance was deteriorated in 38 of 75 patients (51%) and converted to diabetes mellitus in 21 patients (28%). In group BII, 78 among 232 patients (34%) developed diabetes mellitus. Furthermore, in 85% of the 99 cases of the conversion to diabetes mellitus from borderline diabetes, it occurred within 5 years. Diabetic retinopathy was observed in 25 of 72 patients (35%) in group BI and in 60 of 212 patients (28%) in group BII. More than two thirds of the patients with diabetic retinopathy observed in the borderline diabetes developed their retinopathy within 5 years. Normal insulin response during GTT was proved only in 21% and 17% among groups BI and BII, respectively. These results indicate that the patients with borderline diabetes, BI as well as BII, are closely related with diabetes mellitus, and that the patients with impaired glucose tolerance should be treated with diet restriction as in diabetes mellitus. - borderline diabetes; diabetic retinopathy; glucose tolerance test; diagnostic criteria; insulin responseView full abstractDownload PDF (817K) -
HIROSHI ASAKAWA, HIROKAZU OTAWA, SHOGO YAMADA, KO MATSUMOTO1982 Volume 137 Issue 4 Pages 445-452
Published: 1982
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSASAKAWA, H., OTAWA, H., YAMADA, S, and MATSUMOTO, K. Combination Therapy of Gastric Carcinoma with Radiation and Chemotherapy. Tohoku J. exp. Med., 1982, 137 (4), 445-452 - The concurrent combination therapy of radiation and chemotherapy was performed in a total of 134 cases of stomach cancer. Radiation response of tumor was remarkable in 35 (37%) of 95 cases, irradiated more than 5, 000 rad. Yearly survival rates in 81 cases, in which the scheduled curative treatment was completed, were 63% in one, 31% in two, 21% in three, 17% in four and 13% in five years. These rates were intimately correlated to tumor size and cancer type. However, this combination therapy accompanied some fatal complications in a few percent. From the results, it was concluded that this combination therapy should be valuable to prolong the life of patients with gastric cancer, and that the curable indications for this treatment should be T1-T3: M0 cases with radio-responsive tumor. -radiotherapy; chemotherapy; gastric carcinomaView full abstractDownload PDF (795K) -
KENICHI KIMURA, TAKAYOSHI TOYOTA, MASAEI KAKIZAKI, MIKIHIKO KUDO, KAZU ...1982 Volume 137 Issue 4 Pages 453-459
Published: 1982
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKIMURA, K., TOYOTA, T., KAKIZAKI, M., KUDO, M., TAKEBE, K. and GOTO, Y. Impaired Insulin Secretion in the Spontaneous Diabetes Rats. Tohoku J. exp. Med., 1982, 137 (4), 453-459 - Dynamics of insulin and glucagon secretion were investigated by using a new model of spontaneous diabetes rats produced by the repetition of selective breeding in our laboratories. The perfusion experiments of the pancreas showed that the early phase of insulin secretion to continuous stimulation with glucose was specifically impaired, although the response of the early phase to arginine was preserved. The glucose-induced insulin secretion in the nineth generation (F8) which had a more remarkably impaired glucose tolerance was more reduced than in the sixth generation (F5). No significant difference of glucagon secretion in response to arginine or norepinephrine was noted between the diabetes rats and control ones. The present data indicate that the defective insulin secretion is a primary derangement in a diabetic state of the spontaneous diabetes rats. This defect in the early phase of glucose-induced insulin secretion suggests the specific impairment of the recognition of glucose by the pancreatic β-cells. The spontaneous diabetes rats are very useful as a model of a disease for investigating pathophysiology of non-insulin dependent diabetes mellitus.View full abstractDownload PDF (597K) -
KENJI RINSHO, KAZUMASA AOYAGI1982 Volume 137 Issue 4 Pages 461-462
Published: 1982
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSRINSHO, K. and AOYAGI, K. Urinary Hydroxyproline Excretion as a Marker of Bone Metastasis in Prostatic Cancer. Tohoku J. exp. Med., 1982, 137 (4), 461-462 - In 25 patients with prostatic cancer and 26 patients with benign prostatic hypertrophy, urinary hydroxyproline excretion was estimated by the method described by Cleary and Saunders. The patients were kept on a diet free of gelatin. Urinary creatinine was measured in all patients and serum acid phosphatase, prostatic acid phosphatase, alkaline phosphatase and calcium were measured in patients with prostatic cancer. Urinary hydroxyproline excretion and the hydroxyproline/creatinine ratio were both elevated in patients who had prostatic cancer with bone metastasis when compared to values in patients who had benign prostatic hypertrophy or prostatic cancer without bone metastasis. These two were more sensitive indicators of bone metastasis than serum acid phosphatase, prostatic acid phosphatase, alkaline phosphatase and calcium. These results suggest that urinary hydroxyproline is a valuable index of bone metastasis in prostatic cancer.View full abstractDownload PDF (180K) -
TAMOTSU TAKISHIMA, SHUNSUKE SUZUKI, KIYOHISA SEKIZAWA, YOSHIO HIROSE, ...1982 Volume 137 Issue 4 Pages 463-464
Published: 1982
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSTAKISHIMA, T., SUZUKI, S., SEKIZAWA, K., HIROSE, Y., SASAKI, H., SUGIYAMA, M., SHIMIZU, Y., AKAIZAWA, Y. and SHINDO, C. Laryngeal Narrowing Measured with Low Frequency Sound. Tohoku J. exp. Med., 1982, 137 (4), 463-464 -We measured laryngeal resistance with 800Hz sound in human subjects. 800Hz sound was forced into the mouth and the intensities of sound above and below vocal cord were detected by two microphones on the anterior neck. Respiratory resistance (Rrs) was simultaneously measured by an oscillation method. When the subject voluntarily controlled the glottis aperture at functional residual capacity, the percent increase of difference of intensity of sound between two microphones (Y%) was curvilinearly proportional to the increase of Rrs (X) from the control state; Y=20.8×X1.24. Since Rrs below the larynx was constant at a given lung volume, we could estimate the increase of laryngeal resistance from the control state by knowing the percent increase of difference of intensity of sound. The present method would be clinically applicable to measure Rrs independent from laryngeal artifact.View full abstractDownload PDF (218K)
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