All issues
Volume 99 (1969)
- Issue 4 Pages 309-
- Issue 3 Pages 207-
- Issue 2 Pages 107-
- Issue 1 Pages 1-
Volume 99, Issue 1
Displaying 1-13 of 13 articles from this issue
- |<
- <
- 1
- >
- >|
-
Takeshi Yoshida, Nobuo Ishihara1969 Volume 99 Issue 1 Pages 1-8
Published: 1969
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe level of succinyl-CoA synthetase in rat liver mitochondria was not appreciably increased by the administration of allylisopropylacetamide (AIA), while the activity of δ-aminolevulinate synthetase in liver mitochondria was increased more than ten times in these rats. The synthesis of δ-aminolevulinate by liver mitochondria was significantly increased by the addition of ATP in the assay system. The ATP-stimulated increase of the δ-aminolevulinate synthesis in mitochondria from control rats was completely abolished by the further addition of atractyloside, but the ATP-stimulated synthesis of δ-aminolevulinate by mitochondria from AIA-treated rats was far less sensitive to atractyloside. The atractyloside barrier itself appeared to be not affected by the AIA administration as far as examined with respect to the α-ketoglutarate oxidation system and the ATPase system.View full abstractDownload PDF (451K) -
Yoichiro Sasai, Tadaaki Fujiyama1969 Volume 99 Issue 1 Pages 9-17
Published: 1969
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSTissue changes in epidermolysis bullosa acquisita were investigated by histological and histochemical methods. In an attempt to identify acid mucopolysac-charides, staining was carried out with alcian blue solutions containing different concentrations of electrolytes. Methylation, saponification and digestion by hyaluronidase, sialidase and diastase were also applied. The alteration of collagen was examined by the luxol fast blue, trichrome and sulfation toluidine blue methods. The most striking abnormalities of the skin, whether clinically involved or not, were an increase of dermatan sulfate and a decrease of luxol fast bluepositive collagen and oxytalan fibers in the upper dermis. The pathogenesis of epidermolysis bullosa acquisita was discussed on the basis of these results.View full abstractDownload PDF (3917K) -
Kazukuni Yamashita1969 Volume 99 Issue 1 Pages 19-23
Published: 1969
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe effect of anthraquinone derivatives (anthraquinone, sodium anthraquinoneβ-sulfonate, anthrarufin and anthrone) and other compounds (α-naphthoquinone, lecithin and tannic acid) on progestational proliferation produced by progesterone in the rabbit uterus was investigated by measurements of endometrial carbonic anhydrase activity.
In immature rabbits primed with estrogen, the subcutaneous administration of progesterone (2mg/animal for 5 days) caused a typical activation of endometrial carbonic anhydrase. Of the test compounds used, oral treatment with anthrarufin in company with progesterone inhibited the enzyme activation by progesterone. The most effective dose was 1.0mg, causing a 50% inhibition to the action of 2mg progesterone. When given subcutaneously together with progesterone, anthrarufin caused little or no inhibition of the progesterone-induced enzyme activation. Oral administration of anthraquinone, sodium anthraquinone-β-sulfonate, α-naphthoquinone. lecithin and tannic acid did not interfere with the endometrial response to progesterone. It thus appears that in the rabbit oral administration of anthrarufin causes a significant inhibition of the progesterone-induced increase in endometrial carbonic anhydrase activity and has an antiprogestational action.View full abstractDownload PDF (308K) -
Takeo Kuroyanagi, Hajime Sugiyama1969 Volume 99 Issue 1 Pages 25-33
Published: 1969
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe direct anti-globulin test was performed at random on 310 hospitalized patients with various diseases. Thirty-three patients were positive to anti-globulin test without any evidence of manifest autoimmune hemolytic anemia.
Hematologic and erythrokinetic studies on these patients with latent autoimmune hemolytic syndrome revealed that their slight anemia was secondary anemia due to their basic diseases with a slightly increased hemolysis.
The basic diseases of these patients were autoimmune diseases, mesenchymal disease, chronic infections, hypersensitivity disease, pulmonary silicosis associated with tuberculosis, leukemia, Hodgkin's disease and others.
Anti-erythrocyte autoantibodies were of warm type and intermediate type except for one case of γ-globulin type. The immunoglobulin class of anti-erythrocyte autoantibodies was IgG. Their light chain type was as follows: K type, 2; λ type 5; and K+λ type, 1. The coexistence of other various autoantibodies was demonstrated in most of the patients.
A marked increase of peripheral large lymphocytes, hyperplasia of germinal centers associated with proliferations of reticulum cells and lymphogonias (immunoblasts) and a marked infiltration of plasma cells were demonstrated in biopsied lymph nodes.
The mechanism of the association of latent au toimmune hemolytic syndrome with basic diseases was discussed.View full abstractDownload PDF (447K) -
Hiroshige Kurokawa, Syusaku Katsura, Mitsugi Kamada, Wataru Matsuo, Ka ...1969 Volume 99 Issue 1 Pages 35-44
Published: 1969
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSAn 8-year-old girl with myelocytic leukemia was found to have blood group B by detection of B agglutinogen and anti-A agglutinin, agglutinin absorption test, elution test and family studies, but her red cells not agglutinated by anti-B serum amounted to 83% in the peripheral and bone marrow blood, and the red cells failed to react with anti-H.
By the microprecipitation method, HbF-cells were found to occupy 79.8% of the whole red cells and 87.9% of the non-agglutinated red cells at the first examination. Thirty-seven days later, fetal hemoglobin containing red cells were 78.1% of the whole red cells, 87.1% of the non-agglutinated red cells, 58.2% of the agglutinated ones and 88.8% of the non-agglutinated red cells treated with bromelin. The quantity of HbF tested by the alkali denaturation method amounted to 54.8% of the non-agglutinated and 28.8% of the agglutinated red cells.
HbF components were increased and carbonic anhydrase was reduced to a low level in the patient's blood.View full abstractDownload PDF (2329K) -
Hiroshi Shiono, Kimie Bandou, Junichi Kadowaki1969 Volume 99 Issue 1 Pages 45-50
Published: 1969
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe chromosomal and dermatoglyphic studies were carried out on nine cases of Waardenburg's syndrome.
1) The shortening of the palpebral fissures on the horizontal direction (blepharophimosis), at first glance, gives the characteristic impression. Out of 9 patients 3 were without this feature.
2) We supported Feingold et al. s view that the whole characteristic features of Waardenburg's syndrome were not present at birth in some cases and either appeared or disappeared during their life.
3) All the depigmented sides of iridis were not parts of the affected deafness.
4) Two patients were born from parents over thirty years old. The pregnancies of all patients were of full term and normal in 5 individuals, and there was no low-birth-weight baby at full term.
5) With regard to complication, there was only one individual who was clinically diagnosed as interventricular septal defect.
6) The chromosomal constitution was definitely normal.
7) The dermatoglyphic analyses in patients and their families revealed that the frequencies in both the arch patterns and the simian lines were increased, and that those in the total ridge counts were decreased. The frequencies in the right third interdigital loop and the right thenar loop were higher in patients than in normal.View full abstractDownload PDF (343K) -
Satoru Matsushita, Lie Hon Ying, Jiro Kaneko, Kazuhiko Murata, Jun Fuj ...1969 Volume 99 Issue 1 Pages 51-57
Published: 1969
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSVasodilatation and vasoconstriction were produced in the crossperfused kidney of anesthetized dogs by intravenous injection of noradrenaline or stimulation of the cervical efferent vagus nerve. The vasomotor responses were evaluated by recording the renal blood flow with an electromagnetic flowmeter together with the systemic arterial pressure and perfusion pressure. Ifydergine and β-TM10 abolished the constrictor and dilator responses, and there was no apparent tendency of specific blocking of either dilator or constrictor response in small doses. Atropine, propranolol, and hexamethonium failed to block the vasodilator response. It is concluded that the only mechanism for eliciting the renal vasodilatation is inhibition of sympathetic constrictor discharge.View full abstractDownload PDF (914K) -
Tsuneo Arakawa, Takashi Mizuno, Mobukazu Chida, Kuniaki Narisawa1969 Volume 99 Issue 1 Pages 59-64
Published: 1969
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSFolic acid deficiency during the suckling period was induced by intraperitoneal injections of aminopterin to both lactating dams and offsprings in the following way; in the case of lactating rats, aminopterin 0.038mg/kg. 0.038mg/kg and 0.076mg/kg, was given on the 4th, 10th and 18th days of delivery, respectively, and in the case of offpsrings, aminopterin, 0.038mg/kg, was given only on the 18th day of life.
At the age of 6 weeks rats thus treated were examined for DNA and RNA content of the brain, lipids in myelin fraction, and analyzed for basic waves of EEG.
The results revealed that, in 6-week-old rats with the aminopterin-treatment during the suckling period, there was a significant decrease in both cholesterol and phospholipids contents of myelin fraction and a definite delay of the maturation pattern of the EEG basic waves.View full abstractDownload PDF (325K) -
Takuro Ogata, Fumino Murata1969 Volume 99 Issue 1 Pages 65-71
Published: 1969
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe normal rat gastric mocosa was fixed in 10% formalin, dehydrated in acetone, dried in air, coated with gold and examined under a JMS-2-type scanning electron microscope. The surface structure of the gastric mucosa is well preserved in this process. The surface cells are arranged rather like a bunch of grapes and microvilli are seen as tiny processes. The process of dehiscence of the surface mucous cell membrane for the secretion of mucous granules is observed in a three-dimensional aspect.
In addition, the study of the surface epithelium after pilocarpine administration reveals much more widely opened gastric pits in comparison with the control.View full abstractDownload PDF (2419K) -
Tadashi Igari, Masao Tsuchizawa, Kijuro Obara, Shigeru Ono1969 Volume 99 Issue 1 Pages 73-80
Published: 1969
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSTryptophan and its intermediate metabolites in the synovial fluid were studied in order to know a specificity of the tryptophan metabolism in rheumatoid arthritis and osteoarthritis. A metabolic map was made, and the pathways of tryptophan breakdown in both diseases were compared. It was found that tryptophan in rheumatoid arthritis was metabolized mainly to anthranilic acid through kynurenine, whereas in osteoarthritis the pathway of kynurenic acid and nicotinic acid was characteristic.
Tryptophan in the blood and the synovial fluid, and intermediate tryptophan metabolites in the synovial fluid were determined for the purpose of examining the specificity of tyrptophan metabolism in joint diseases.
1) The fluorescent spots in the concentrate of the synovial fluid, namely anthranilic acid, xanthurenic acid and 3-hydroxykynurenine derivative and kynurenine, were found in both diseases.
2) The tryptophan content in the synovial fluid in rheumatoid arthritis was found to be higher than that of osteoarthritis, but no change was found in the blood of joint diseases.
3) No relation was found between the tryptophan content in the blood and theexistence of joint hydrops in both diseases and also among the tryptophan content in blood and synovial fluid and stages in rheumatoid arthritis.
4) The closest correlation was found between the tryptophan content in the synovial fluid and the erythrocyte sedimentation rate in rheumatoid arthritis.
5 Among the tryptophan intermediates in synovial fluid, kynurenine and anthranilic acids were higher in rheumatoid arthritis than those in osteoarthritis, but kynurenic acid and nicotinic acid contents in rheumatoid arthritis were found to be about half those in osteoarthritis. No difference was found in xanthurenic acid content between the two diseases.View full abstractDownload PDF (422K) -
Aiko Kaneko, Shinichi Sakamoto, Masahiko Morita, Tamenori Onoé1969 Volume 99 Issue 1 Pages 81-101
Published: 1969
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe early changes in the rat liver cells after the administration of ethyl chlorophenoxyisobutyrate (CPIB) were studied. At first, mitochondrial changes appeared 6 to 12 hours after the first dose of CPIB; the mitochondria were deformed and the succinic oxidase activity decreased; these changes disappeared in 24 hours. At 12 hours after administration an increase in smooth endoplasmic reticulum was observed and this proliferation reached the maximum on the 4th day accompanying increase of activity of drug metabolizing enzyme system. After 24 hours increased formation of microbodies without nucleoids followed the appearance of smooth ER and it was parallel to the increase of enzyme activities associated with microbodies, catalase and D-amino acid oxidase. These augmentations attained the plateau after 4 days. On the other hand, CPIB caused less remarkable change in lysosomes.
A consistent increase in the liver weight of rats receiving CPIB was found and this rise was not accompanied by any histological evidence of liver damages. easurements of protein, RNA and DNA show that the increase in liver weight is not due to the increase in the number of cells, but to the increase of the protein content in cytoplasm.
The relationship among intracellular organelles on the effect of CPIB was discussed.View full abstractDownload PDF (5768K) -
Shigetoshi Chiba, Keitaro Hashimoto, Koroku Hashimoto1969 Volume 99 Issue 1 Pages 103-104
Published: 1969
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSDownload PDF (127K) -
Masao Endoh, Koroku Hashimoto1969 Volume 99 Issue 1 Pages 105-106
Published: 1969
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe anterior papillary muscle of the canine right ventricle was perfused at a constant pressure of 100mm Hg with the blood from the donor dog by a crosscirculation technique. Temperature was kept constant at 38 to 39°C. Isometric tension was measured by a force displacement transducer. The muscle was electrically stimulated at the voltage approximately twice the threshold (0.5 to 1.5 volts), 5 msec and at variable frequencies from 36 to 360min, because the muscle contracted spontaneously at a rate of 20±3 beats/min (mean±s. E.; n=10). Contractile force was markedly increased by raising the stimulation frequency. The rate of spontaneous contraction, stimulation threshold, and frequency-force relationship were not significantly changed in the course of experiments of 10 to 20 hours.
None of atropine, β-adrenergie blocking agent and tetrodotoxin modified the frequency-force relationship.
These results demonstrate that the frequency-force relationship is a characteristic property of the ventricular muscle itself and there is no participation of the autonomic nerve stimulation.View full abstractDownload PDF (138K)
- |<
- <
- 1
- >
- >|