1) Urinary estrogen excretion of climacteric women with uterus cancer was found in a comparatively large quantity and there were individual differences. 2) Urinary estrogen excretion of climacteric women after post-oophorectomy decreased temporarily, increased afterwards, and 12 days leter the post-operation estrogen was found to decrease again. Of the estrogen fraction, the decrease of estradiol was the clearest. 3) The greater part of estrogen extracted from the rabbit urines was about estradiol fraction. The mean value of estrogen excretion 28 days after oophorectomy was about a half of the pre-operation level. After adrenalectomy besides, estrogen decreased apparently, but a small amount of estrogen could be detected now in the urine. 4) Blood estrogen of adult female rabbits indicated the mean value of 2.35±0.24 μg per serum 10ml, after oophorectomy decreased to 1.56 μg and there were significant differences. However, the decrease rate of free and protein bound estrogen was about the same. 5) In this test it was affirmed that there were comparatively large amounts of estrogen in rabbit brain, lung, liver, kidney, bowel, uterus, muscle and fat. 6) The estrogen contained in rabbit fat, muscle, kidney and uterus decreased markedly after oophorectomy, especially, the decrease of estrogen contained in fat was most prominent. 7) It was concluded that no estrogen production occurs in rabbit uterus and kidney, but that the conversion of testosterone to estrogen occurs.
1. In the present paper, animal experiments of the chemotherapy with Citronellal in MH 134 (adenocarcinoma) of C3H FI strain are reported. 2. About 30 to 50% of tumor animals were cured when the chemotherapy with Citronellal was started on the same day as inoculation of tumor. 3. When the administration of Citronellal was begun on the third day of tumor inoculation, the result of chemotherapy was lower, yet the effect was quite obvious. In this case the efficacy was very much promoted with the combined use of PCMB. 4. Our expectation in the co-operation of PCMB with Citronellal and Citral, based upon the electron microscopic study of tumor cells mentioned in the previous paper, is verified in this report.
Based on instrumental conditioned avoidance responses, retention and acquisition of visual discrimination of flicker frequencies were studied in 15 dogs with surgical lesion limitted to the bilateral neocortecies of polus temporalis and gyrus temporalis inferior. In addition, the differential threshold of visual performance, i.e., the least difference of flicker frequencies to be discriminated was determined at various pre- and postoperative stages in order to investigate the capacity to form learning sets, i.e., learn how to learn. Histological control of the ablated areas was made by a conventional method. The results obtained are as follows: 1. The retention of the preoperatively learned discrimination was markedly reduced by bilateral ablation of the inferotemporal cortex, while it was not affected in the control animals which underwent bilateral removal of gyrus ectosylvius or unilateral lesion of the inferotemporal lobe. 2. For the inferotemporal-operated dogs, learning was further more difficult than for the control dogs. 3. Impairments in retention and acquisition were more marked, when tested with more difficult tasks. 4. The differential threshold of visual performance was remarkably impaired by ablation of the inferotemporal lobes, but it recovered to the preoperative level after a long period of training. It was, however, not affected by the control operation. 5. The inferotemporal-operated dogs showed some kind of spatial disorientation, while the control dogs did not. 6. The significance of the inferotemporal lobe for visual performance was discussed.
Two families of congenital nonspherocytic hemolytic anemia were reported. A metabolic abnormality in the erythrocytes from the patients was demonstrated, consisting of a low content of GSH, an abnormality in the GSH stability test and a low activity of G6PD. These abnormalities were also found in the erythrocytes from the mothers in a milder degree, while there was no abnormalities in the erythrocytes from the fathers. An aggravation of hemolysis and metabolic disturbances mentioned above was observed following administration of Domian (6-sulfanilamido-2, 4-dimethylpyrimidine) to the patient. It was also argued by way of experiment that the difference between the so-called congenital nonspherocytic hemolytic anemia and drug-induced hemolytic anemia is not essential, but only quantitative, so that both disorders belong highly probably to the same entity of enzymopathy fromm the viewpoint of pathogenesis of hemolysis.
Basing upon the observations of hemolytic effect by the administration of Domian to the patients with erythrocytes deficient in G6PD and in GSH, a relationship between sulfa-drug and metabolism in erythrocytes was investigated. The experimental results obtained are as follows. 1) Domian has in vitro an inhibitory effect on G6PD activity but no effect on glutathione reductase activity. 2) A series of sulfa-drugs have in vitro a similar effect on G6PD activity as that by Domian. 3) Inhibition of G6PD activity by Domian may be noncompetitative against glucose-6-phosphate. 4) Inhibition rate of G6PD activity by Domian tends to decrease in proportion with increase of concentration of the enzyme. 5) Domian has in vitro no effect on oxidation of GSH. 6) Domian has in vitro no effect on oxidation of TPNH or DPNH. 7) The hemolysis of human erythrocyte caused by incubation with Salyrgan is completely protected by addition of an equimolar solution of GSH.
Enzymological studies on serum, erythrocytes and muscle were carried out in two patients with progressive muscular dystrophy. The activities of serum aldolase, transaminase and LD were remarkably elevated. The activities of the erythrocyte enzymes such as aldolase, LD and G6PD were within normal limits. The contents of ATP and PN in erythrocytes showed no abnormal values. The glucose uptake test by the erythrocytes revealed normal findings. Analytical results of the muscle revealed that the activity of creatinephosphokinase and the contents of creatine, creatinine and creatine phosphate were definitely lower, while the content of glycogen and ATP and the activities of aldolase, transaminase and LD did not differ much from those of controls in the values per 1.0g of nitrogen in the muscle.