The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
Volume 28, Issue 4-5
Displaying 1-16 of 16 articles from this issue
  • 63rd Report of the Peroxidase Reaction
    Akira Sato, Tamotsu Suzuki, Ryoji Shibata
    1936 Volume 28 Issue 4-5 Pages 285-288
    Published: April 19, 1936
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    In the present paper it has been shown how abnormal or pathological leucocytes, peroxidase-negative as well as peroxidase-positive, and nucleated red cells will appear under the original copper peroxidase reaction of Sato-Sekiy a and under the Tohoku-Pediatric Method, a modification of the original. And it has been shown how easily the abnormal leucocytes can be differentiated with these two methods, especially with the Toll oku-Pediatric Method.
    Download PDF (197K)
  • 64th Report of the Peroxidase Reaction
    Tamotsu Suzuki, Masayuki Shinto
    1936 Volume 28 Issue 4-5 Pages 289-298
    Published: April 19, 1936
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    In the present work it has been discussed whether or not blood mast cells are oxidase- and peroxidase-positive.
    1. There has been a discrepancy of opinion as to the question, and a survey of literature has shown that the difference may be due to different techniques of fixation.
    2. We were able to demonstrate that all mast cells are oxidaseas well as peroxidase-positive, or at least so on principle. This was due to a fixation of air-dried blood films with formalin-alcohol. Then these were treated with Sato-Sekiya's peroxidase method resp. with Winkler-Schultze's oxidase method. The oxidase and the peroxidase granules of the large majority of mast cells can not be fixed with copper sulphate solution.
    3. In non-fixed blood smears mast cells were mostly oxidase- as well as peroxidase-negative, though in a case of myeloid leukemia with many basophile myelocytes and promyelocytes some premature mast cells were distinctly oxidase as well as peroxidase positive.
    4. The oxidase picture of mast cells was, even when it was stained to be positive, different from that of the other granulocytes, because the interspace between the basophile granules seemed to be oxidase-stained.
    5. The peroxidase picture of mast cells was, even when it was shown to be positive, quite different from that of the other granulocytes. The colour was not beautifully blue, but yellowish-green.
    Download PDF (480K)
  • Junkichi Asano, Tatsuo Sato
    1936 Volume 28 Issue 4-5 Pages 299-303
    Published: April 19, 1936
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    A clinical rapid micro or super-micro method for urea is described, which will enable one to determine urea in a minute amount of urine, such as 0.1 c. c. or smaller amounts.
    Download PDF (502K)
  • Kenzo Hasimoto, Shuzi Hirohata
    1936 Volume 28 Issue 4-5 Pages 304-307
    Published: April 19, 1936
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    Download PDF (706K)
  • Yukio Yoshizawa
    1936 Volume 28 Issue 4-5 Pages 308-312
    Published: April 19, 1936
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    Download PDF (260K)
  • Seiichi Kato
    1936 Volume 28 Issue 4-5 Pages 313-321
    Published: April 19, 1936
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    Download PDF (435K)
  • 75th Report. Effect of Yakriton to Prevent Scarlatinal Nephritis
    Akira Sato, Matuiti Yoshida, Morie Chiba, Jun Kimura
    1936 Volume 28 Issue 4-5 Pages 322-326
    Published: April 19, 1936
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    We tried in our own 25 cases of typical scarlatina, yakriton therapy for the purpose of prevention of nephritis. And if nepritis would be prevented by means of yakriton, the use of this hormone should be begun, as has been taught by all the 35 cases including the 10 cases reported in the 51st report on yakriton, befor the 4th day of the disease and continued every day up to the third week.
    Download PDF (208K)
  • 76th Report. Effect of Yakriton upon Tuberculin from the Hematological Point of View
    Ryoji Shibata
    1936 Volume 28 Issue 4-5 Pages 327-334
    Published: April 19, 1936
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    A. If Koch's old tuberculin is subcutaneously injected in the amount of 0.1 c. c. per kilo of body weight into normal b-classed rabbits, a definite picture will be produced. The blood picture is described below:
    1. Total white cell count. Up to the 1st or the 2nd hour a leucopenia; then a marked leucocytosis up to the 5th hour.
    2. Lymphatic cells. Up to the 5th hour a marked lymphopenia, absolutely as well as relatively, which becomes marked still more as time passes on.
    3. Myeloid cells. Up to the 5th hour a very marked myelocytosis, absolutely as well as relatively, which becomes marked still more with the passing of time.
    4. Nuclear shift. Cells of Class I reach approximately four or five times the percentage in the 4th or the 5th hour after the injection.
    B. If yakriton is subcutaneously injected in the amount of _??_ R. A. U. per kilo of body weight into normal b-classed rabbits, 30 minutes before Koch's old tuberculin injection (0.1 c. c. per kilo of body weight), the blood picture will be changed as below:
    1. Total white cell count. Up to the 1st or the 2nd hour, normal or a slight leucocytosis. A temporarily marked leucocytosis in the 2nd or the 3rd hour, then up to the 5th hour normal, or, a slight leucocytosis again.
    2. Lymphatic cells. Gradually a lymphopenia, reaching the maximum in the 5th hour. This decrease is not so pronounced as in the case of tuberculin injection alone. Up to the 1st or the 2nd hour no change, or, a slight increase in the absolute number.
    3. Myeloid cells. Gradually a myelocytosis, absolutely as well as relatively, reaching the maximum in the 5th hour. This increase is not so marked as in the case of tuberculin injection alone.
    4. Nuclear shift. Cells of Class I reached, about twice the percentage in the 3rd or the 4th hour. Up to the 1st or the 2nd hour, cells of Class I decrease and those of Types IV and V increase.
    Contrary to what the above mentioned authors state, 1)2)3)4) I have obtained a definite change of blood picture resulting from the injection of old tuberculin. The reason is, I believe, very simple. I have used selectively only b-classed animals. It would have been interesting to do the same experiment in f-classed ones too, but unfortunately I was unable to obtain a sufficient number of such rabbits during the present work.
    Download PDF (345K)
  • 77th Report. Effect of Yakriton upon Urobilinuria and Third Report on Clinical Experiment to Prevent Scarlatinal Nephritis by Yakriton
    Issei Horiuti
    1936 Volume 28 Issue 4-5 Pages 335-348
    Published: April 19, 1936
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    A preliminary report on the prophylactic treatment of scarlatinal nephritis was published in 1929 by Prof. S ato, Yamagi shi, Abe and Yoshida.1) And then a summarizing report on 35 scarlatinal cases was published in 1931 by Prof. Sato, Yoshida, Chiba and Kimura.2) The most important point in the report was that yakriton therapy should be commenced as early as possible in the first week of the disease in order to prevent nephritic complications. It is very clear that their favorable result was not an accidental one, which may well be inferred also from T. Sato's19) paper concerning antinephritic effect of yakriton.
    In the present work I tried to use yakriton not daily, but every second or third day to see whether such a usage would likewise act to prevent nephritic complication. And I found that, if yakriton therapy is begun early in the first week of scarlatina, then its use on every other day will be able to prevent the complication. Besides I made it a point of investigation, whether yakriton would act favorably upon scarlatinal hepatitis or scarlatinal disorder of the liver and counteract urobilinuria, and I found that it would act to decrease urobilin excretion.
    Download PDF (632K)
  • 78th Report. Possibility of Judging Individual Liver Power of Ammonia-Detoxication by Adrenalin; Classification of Liver Power by Use of Yakriton and Adrenalin. The Yakriton-Adrenalin Method
    Akira Takamatsu
    1936 Volume 28 Issue 4-5 Pages 349-356
    Published: April 19, 1936
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    Sato-Sakurada's liver function test based on the individual liver power of ammonia detoxication in the case of rabbits of course cannot be applied to human beings, because the method aims at the development of severe convulsions. It has been my intention to apply the principle of the method to clinical medicine. In the present paper I tried a yakriton-adrenalin method of my own device in the case of rabbits to test whether a direct relation would exist between the Sato-Sakurada's liver function test and my own method. And a rather close relation was found between the two methods, so that β-, γ- and ζ-classed rabbits in my own method corresponded in most cases to b-, c- and f-classed ones in Sato-Sakurada's method. During this experiment, it was found that the ammonia detoxicating liver power was weakest in summer and strongest in autumn. Though the experimet did not happen to be performed in spring, yet the result of the experiment in this season would, as it may be easily imagined, have been almost the same as in autumn.
    Download PDF (355K)
  • Studien über den kolloid-osmotischen Druck des Bluts im normalen und pathologischen Zustand. XX
    Masami Horikawa
    1936 Volume 28 Issue 4-5 Pages 357-375
    Published: April 19, 1936
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    Download PDF (918K)
  • Studien über den kolloid-osmotischen Druck des Blutes im normalen und pathologischen Zustand. XXI
    Masami Horikawa
    1936 Volume 28 Issue 4-5 Pages 376-380
    Published: April 19, 1936
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    Download PDF (252K)
  • Kenkai Ujiie
    1936 Volume 28 Issue 4-5 Pages 381-422
    Published: April 19, 1936
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    Download PDF (2231K)
  • Nach den Versuchen von M. Takano
    Sakuzi Kodama
    1936 Volume 28 Issue 4-5 Pages 423-433
    Published: April 19, 1936
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    Der Gaswechsel der Rattennetzhaut wurde in vitro nach Warburg'scher Methode (1924) gemessen und der Einfluss des Lichtes und der Zuckerkonzentration in der Ringerlösung darauf untersucht.
    Der Sauerstoffverbrauch war sowohl bei lang dauernder als auch bei kurz dauernder Beleuchtung der Lichtstärke nicht proportional. Bei lang dauernder Beleuchtung wurde der Verbrauch im Anfangsstadium von einer 127 Fx Lampe und von der Lichtstärke fördernd beeinflusst, welch letztere durch Schwächung der 2, 25 Fx Lampe mit einer und zwei schwarzen Glasplatten erzeugt wurde, und bei kurz dauernder Beleuchtung von 254, 127, 32 Fx und von der Lichtstärke, welch letztere durch Schwächung der 2, 25 Fx mit einer schwarzen Glasplatte erzeugt wurde.
    Die aerobe Glykolyse wurde so wohl bei lang dauernder als auch bei kurz dauernder Beleuchtung von der Lichtstärke wenig beeinflusst.
    Der Gaswechsel verminderte sich zeitlich in allen Fällen, und zwar war der Abfall beim Sauerstoffverbrauch auffallend gross.
    Von den farbigen Lichtern war der Sauerstoffverbrauch bei Beleuchtung mit rotem Licht am grössten, bei dem grünen am nächsten, und bei dem blauen am geringsten und sogar im Anfangstadium nicht nachweisbar. Die aerobe Glykolyse zeigte im ganzen eine fast gleiche Tendenz wie der Sauerstoffverbrauch, zeitlich aber mit geringerem Abfall.
    In der zuckerfreien Ringerlösung war der Sauerstoffverbrauch (im Dunkeln) anfangs gross, und dann verminderte er sich rasch auf Null. Die anaerobe und aerobe Glykolyse war verschwindend gering. In 0, 1% Zucker-Ringerlösung war der Sauerstoffverbrauch anfangs sehr gross, aber zeitlich verminderte ersich rasch und die aerobe Glykolyse war noch mässig vorhanden. In 0, 4% Zucker-Ringerlösung war der Sauerstoffverbrauch und die aerobe Glykolyse von Anfang an relativ gering, ohne zeitlichen Abfall. Die anaerobe Glykolyse war bei allen 0, 1, 0, 2 und 0, 4% Zucker-Ringerlösung sehr gross in fast gleichem Grad.
    Download PDF (520K)
  • Kenzo Hasimoto
    1936 Volume 28 Issue 4-5 Pages 434-454
    Published: April 19, 1936
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    A review of the studies on the blood alkalinity occourring by the carbon monoxide poisoning and like conditions is given as an introductory, because the findings, in fact the former findings, of the pioneer of the carbon dioxide therapy in respect to the blood alkalinity have very close relation to the recommendation of that therapy.
    The present writer himself, who conducted inhalation of carbon monoxide on rabbits, is able to find also definitely the beneficial effect of mixing carbon dioxide to oxygen for treatment of carbon monoxide poisoning, clearly superior to the pure oxygen. Carbon monoxide is more rapidly eliminated, the oxygen content of blood is more rapidly restored and the carbon dioxide content of blood more rapidly still. The pH of blood, which shows usually the acidosis, inclines to shift further. And all the tendencies increase according to the increase of the content of carbon dioxide in the inhalational mixture.
    With these results we do not mean, contrary to critics, that the alkali administration should act detrimentally against the carbon monoxide poisoning and like conditions. Kamei's work demonstrates it beyond doubt. One must take care, there is little need to say, of its dosage.
    Further it may be said in passing that we will not take the present outcome as unconditionally proving a view of some critic that intensification of acidosis is never harmful to the organism though it is wholly settled that the use of carbon dioxide-oxygen mixture largely accelerates the expulsion of CO from blood, the restoration of the O2, and CO2, content of blood.
    Download PDF (1078K)
  • Kenzo Hasimoto
    1936 Volume 28 Issue 4-5 Pages 455-458
    Published: April 19, 1936
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    On working with the toad, bufo formosus Baulenger, the dorsal spinal roots, the third, fourth and fifth, were stimulated with the motor effect upon the stomach and the lower part of oesophagus. No narcosis was used, and the section was carried out between the medulla oblongata and the spinal cord. The corresponding ventral spinal roots had no effect at all.
    The present investigations therefore designate to revive the view that the dorsal roots carry viscero-motor impulses, which apparently nearly died out through rejection by the Cambridge physiologists, just as did the vasodilator problem.
    Download PDF (222K)
feedback
Top