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Volume 171 (1993)
- Issue 4 Pages 267-
- Issue 3 Pages 185-
- Issue 2 Pages 101-
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Volume 171, Issue 2
Displaying 1-8 of 8 articles from this issue
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TOSHIHIRO ISHII, KUMIKO KASAMA, MAYUMI KONDO, TOHRU TAKAHASHI1993 Volume 171 Issue 2 Pages 101-105
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSISHII, T., KASAMA, K., KONDO, M. and TAKAHASHI, T. Cryostat Sectioning of Formalin-Fixed Brain: Further Attempt to Improve Section Quality by Previous Infiltration with O.C.T. Compound. Tohoku J. Exp. Med., 1993, 171(2), 101-105 - We previously reported that in non-brain and formalin-fixed specimens, one can obtain frozen sections of markedly improved quality not only by supporting specimens with O.C.T. compound as usually done, but by infiltrating them with the medium prior to embedding. In the present paper we show that this technic also proved to be quite effective in the cryosectioning of brain tissues, which so far has been difficult because of the liability of sections to shattering, particularly when one attempts to obtain a thick section. The present paper also discusses about the reason why sectioning is improved by the procedure, and aims to establish the optimal temperature for cryostat sectioning in relation to section thickness.View full abstractDownload PDF (2506K) -
SHIGERU DEGUCHI, YOSHIHIRO MUTO, TOSHIOMI KUSANO, SYUJI TOMITA, TAKAYO ...1993 Volume 171 Issue 2 Pages 107-118
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSDEGUCHI, S., MUTO, Y., KUSANO, T., TOMITA, S. and TODA, T. Intratumoral Heterogeneity of DNA Ploidy and Regional Differences in Epidermal Growth Factor and Epidermal Growth Factor Receptor of Esophageal Carcinoma. Tohoku J. Exp. Med., 1993, 171(2), 107-118 - Intratumoral regional differences in DNA ploidy patterns, and expression of epidermal growth factor (EGF) and EGF receptor (EGF-R) were studied to evaluate the biological and clinical significance of intratumoral DNA heterogeneity in 23 cases of esophageal carcinoma. Multiple specimens were subjected to histologic grading of carcinoma, DNA analysis and immunohistochemistry. DNA heterogeneity was found in 34.8% of the cases. Expression of EGF and EGF-R within a single tumor was observed in 69.6% and 73.9% of the lesions examined, respectively. A positive correlation was noted between the expression of EGF and that of the EGF-R. EGF expression showed positive correlation with the DNA index, but not with the proliferative index. There was no relationship between DNA heterogeneity and the degree of histopathological differentiation of carcinoma. Cases with DNA heterogeneity showed better prognosis than those with DNA homogeneity. The present study suggests that esophageal carcinoma consists of carcinoma cells with intratumoral regional polymorphism and variable types of clones. Furthermore, the autocrine mechanism of EGF and EGF-R could be one of the contributory factors on the development of DNA abnormalities during tumor proliferation.View full abstractDownload PDF (1944K) -
Study on Proper Time for Dynamic Magnetic Resonance Imaging of the Human Kidney on Low and High Magnetic FieldsMASAYUKI TAKEDA, YASUSHI KATAYAMA, TOSHIKI TSUTSUI, TAKESHI KOMEYAMA, ...1993 Volume 171 Issue 2 Pages 119-128
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSTAKEDA, M., KATAYAMA, Y., TSUTSUI, T., KOMEYAMA, T., MIZUSAWA, T., TANIKAWA, T. and SATO, S. Concentration of Gadolinium-Diethylene Triamine Pentaacetic Acid in Human Kidney - Study on Proper Time for Dynamic Magnetic Resonance Imaging of the Human Kidney on Low and High Magnetic Fields. Tohoku J. Exp. Med., 1993, 171(2), 119-128 - Although Gadolinium diethylene Tramine pentaacetic acid (Gd-DTPA) has been used as a contrast material in magnetic resonance imaging, it is known that contrast enhancement effect disappears if the concentration of Gd-DPTA increases beyond some levels. In this study, to evaluate the proper pulse sequences for dynamic magnetic resonance imaging (MRI) in the human kidney, the concentration of Gd-DTPA was quantitatively measured by inductively coupled plasma (ICP) emission spectrometry in human biological samples after administration of Gd-DTPA, and the signal intensity of MRI is the solutions of several concentrations of Gd-DTPA was measured. The results were; 1. In using a low magnetic field apparatus, signal intensity linearly correlated with the concentration of Gd-DTPA between 0 and 2.0μmol/g under saturation recovery sequences (flip angle was 6O° or 9O°). Using a high magnetic field apparatus, signal intensity linearly correlated with the concentration of Gd-DTPA between 0 and 2.0 or 3.0μmol/g under spin echo or gradient-echo sequences. 2. Gd-DTPA concentration of the renal cortex ranged from 0.132 to 0.152μmol/g tissue at 5min after intravenous injection of Gd-DTPA 0.05mmol/kg body weight in 7 patients with adrenal tumor or renal cell cancer, and 1 patient with both urinary bladder cancer and prostatic cancer. Seven of them showed normal renal function and the other had renal insufficiency (GFR 25 ml/min/1.48m2). Gd-DTPA concentrations of renal medulla and renal cell cancer tissue were 0.123 and 0.108μmol/g tissue, respectively, at 5min after intravenous injection of Gd-DTPA 0.5mmol/kg body weight.View full abstractDownload PDF (964K) -
KAZUYA KITAMURA, TOSHIO TAKAHASHI, HIROKI TANAKA, MASATAKA SHIMOTSUMA, ...1993 Volume 171 Issue 2 Pages 129-133
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKITAMURA, K., TAKAHASHI, T., TANAKA, H., SHIMOTSUMA, M., HAGIWARA, A., YAMAGUCHI, T. and HASHIMOTO, S. Two Cases of Thiamine Deficiency-Induced Lactic Acidosis during Total Parenteral Nutrition. Tohoku J. Exp. Med., 1993, 171(2), 129-133 - Two cases of severe lactic acidosis induced by total parenteral nutrition (TPN) are reported. Both cases were admitted to our department for the surgical treatment of advanced gastric cancer, and subsequently underwent TPN because of poor nutritional status. Following the initiation of TPN, both patients went into an unstable circulatory state following shock. Both cases showed signs of peritonitis, suggestive of an intraabdominal abscess, and subsequently underwent an emergency laparotomy to explore the origin of the lactic acidosis. There was, however, no apparent infectious focus which would lead to severe metabolic acidosis in the abdominal cavity. One case died of irreversible shock. The other case similarly exhibited a deteriorated cardiovascular state but promptly responded to the administration of thiamine and was resuscitated. The blood thiamine level was low in both individuals, and the two patients were subsequently diagnosed as having thiamine deficiency-induced lactic acidosis.View full abstractDownload PDF (498K) -
MINORU YASUJIMA, KAZUNORI YOSHIDA, MASAYUKI KANAZAWA, MASAHIRO KOHZUKI ...1993 Volume 171 Issue 2 Pages 135-144
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSYASUJIMA, M., YOSHIDA, K., KANAZAWA, M., KOHZUKI, M., OMATA, K., WATANABE, H., HIWATARI, M., SATO, T. and ABE, K. Urinary Active Kallikrein Excretion and Diabetic Renal Impairments in Streptozotocin-Treated Rats. Tohoku J. Exp. Med., 1993, 171(2), 135-144-To assess possible roles of the renal kallikrein-kinin system in the development of renal impairments in diabetes mellitus, we determined daily excretion of urinary total and active kallikrein in uninephrectomized Wistar-Kyoto rats made diabetic by streptozotocin (45mg/kg) as a bolus injection. We also evaluated the effect of captopril (50mg/kg/day) administered orally on the development of diabetic renal impairments in the streptozotocin-treated rats. Active kallikrein was determined by its kininogenase activity, and generated kinins were radioimmunologically measured. Total kallikrein was also determined by measuring kininogenase activity after inactive kallikrein had been activated with trypsin (200μg/ml). Urinary active kallikrein excretion was significantly reduced in streptozotocin-treated rats whereas urinary total kallikrein excretion was unchanged, resulting in the decreased ratio of active to total kallikrein compared to that in the controls. These reductions were preceded by the increased excretion of urine protein measured as an index of renal impairments. The administration of captopril for 12 weeks attenuated the development of diabetic renal impairments evaluated by urine protein excretion in streptozotocin-treated rats, although it did not induce significant changes in urinary total and active kallikrein excretion, and the ratio of active to total kallikrein. Thus the results of this study indicate that the renal kallikrein-kinin system might not play major roles in the development of diabetic renal impairments in the rat, although the pathophysiological relevance of impaired activation of renal kallikrein system to the development of diabetic renal impairments remains to be determined. In addition, they suggest that the renoprotective effects of captopril may, be independent of the activation of renal kallikrein system in streptozotocin-treated rats.View full abstractDownload PDF (1025K) -
AKIHIRO MUNAKATA, SHIGEYUKI NAKAJI, HIDEKI TAKAMI, HITOSHI NAKAJIMA, S ...1993 Volume 171 Issue 2 Pages 145-151
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSMUNAKATA, A., NAKAJI, S., TAKAMI, H., NAKAJIMA, H., IWANE, S. and TUCHIDA, S. Epidemiological Evaluation of Colonic Diverticulosis and Dietary Fiber in Japan. Tohoku J. Exp. Med., 1993, 171(2), 145-151 - The incidence of colonic diverticulosis was examined in 5 hospitals geographically isolated from each other in Japan during the period between mid '70s and 1986. The incidence rapidly increased in all hospitals from 3.5-9.0% in mid '70s to 8.4-23.2% in 1986. The ratio of right-sided type colonic diverticulosis was approximately 70-80% in each hospital and the ratio did not fluctuate significantly during the period. Birth cohort analysis revealed that although the incidence of right-sided diverticula had increased with aging, in groups born in more recent decades right-sided diverticula appeared in younger age than other groups. It was suggested that environmental factors rather than congenital factors may cause the increase of the incidence of right-sided colon diverticula.View full abstractDownload PDF (1914K) -
YOSHIHARU HOSHIYAMA, TAKESHI SEKINE, TAKAFUMI SASABA1993 Volume 171 Issue 2 Pages 153-165
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSHOSHIYAMA, Y., SEKINE, T. and SASABA, T. A Case-Control Study of Colorectal Cancer and Its Relation to Diet, Cigarettes, and Alcohol Consumption in Saitama Prefecture, Japan. Tohoku J. Exp. Med., 1993, 171(2), 153-165 - A case-control study of colorectal cancer in relation to dietary, smoking, and drinking habits was undertaken in Saitama Prefecture, Japan. The study was based on 181 newly diagnosed cases of adenocarcinoma of the colorectum at a single institution and 653 general population controls. Dietary habits were investigated on the basis of the intake of 12 foods and 12 food groups in a food intake frequency questionnaire, together with individual food preferences. Preference for salty foods was positively related to the risk of both colon and rectal cancer, and the consumption of seaweed was inversely related to these cancers, both with a dose-response relation. Cigarette smoking was inversely related to colon cancer risk, but not to rectal cancer risk. Alcohol intake tended to be associated inversely with colon cancer, but not with rectal cancer. In the multiple logistic regression, preference for salty foods (positively) and the consumption of seaweed (inversely) were independently related to both colon and rectal cancer risks.View full abstractDownload PDF (1167K) -
MAMORU ISEMURA, TAKASHI KAZAMA, KUNI TAKAHASHI, YU YAMAGUCHI1993 Volume 171 Issue 2 Pages 167-183
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSISEMURA, M., KAZAMA, T., TAKAHASHI, K. and YAMAGUCHI, Y. Immunochemical Localization of Integrin Subunits in the Human Placenta. Tohoku J. Exp. Med., 171(2), 167-183 - Localization of integrins in the human placenta was examined in comparison with those of fibronectin and laminin. In the early chorionic villi, the α5 and β1 subunits were localized on the basal surface of villous cytotrophoblastic cells, while the α6 and β4 subunits were distributed around the entire surface of the cytotrophoblastic cells, suggesting that these cells attach to fibronectin rather than laminin on the epithelial basement membrane. In the term placenta, the α5 subunit was not detected on the epithelial cells, but the β6 and β4 subunits were detected on the basal surface of syncytiotrophoblastic cells, suggesting that these cells make use of α6 β4 integrin to attach to the trophoblastic basement membrane. These results suggest that cells utilize the specific receptor for their attachment in correspondence to the extracellular matrix component to which they attach.View full abstractDownload PDF (14791K)
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