The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
Volume 70, Issue 3
Displaying 1-8 of 8 articles from this issue
  • Yoshinobu Ishikawa, Sakari Sato, Sbinao Kakizaki, Jin Takita
    1959 Volume 70 Issue 3 Pages 213-219
    Published: August 25, 1959
    Released on J-STAGE: November 28, 2008
    1) Skin homotransplantation was performed in the fourth to fifth week after birth of uniform dd-strain mice which had been injected subcutaneously with the whole blood or spleen cell suspension obtained from the prospective donors (C 57 BL/6 Ms mice) 8 to 24 hours after birth. It was successful in considerably high percentage. Homografts survived for a long period of time and the growing of black hair inhered in the donor was also observed in many cases.
    2) Subcutaneous injection of the cells from homologous origin was effective even after 16 to 18 days after birth.
    3) If the recipients were X-irradiated shortly after birth, the tolerance range was prolonged evidently against controls without irradiation.
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  • Eizo Yamashita
    1959 Volume 70 Issue 3 Pages 221-233
    Published: August 25, 1959
    Released on J-STAGE: November 28, 2008
    A KCl-filled microelectrode was inserted into toad's inverted retina to record slow potentials to microillumination or to even illumination.
    1. Responses obtained from an inverted retina was compared with those recorded from an opened eyebulb, and from this experiment it was suggested that the pigment epithelium contributes to the c-wave of ERG.
    2. Responses were divided into two types; positive and negative. It was possible to obtain the positive type by giving a slight damage to the receptor surface. The negative type was obtainable more easily in light adaptation than dark adaptation.
    3. Properties of focal responses to microillumination were examined. In experiments in which a mechanical cut or twin stimulus spots were used it was confirmed that the spread of potential over 1mm. from the illuminated focus was not due to stray light. The latency of the response obtained at the illuminated spot was found shorter than that of the response obtained from a point of the surrounding area, as evidenced by comparison of the two records or by Lissajous' figure.
    4. Slow potentials of positive and negative types were recorded from different layers of the retina and compared with similar experiments by previous workers.
    5. It was discussed on the origin of each component of ERG.
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  • Takeo Kuroyanagi, Akira Kurisu
    1959 Volume 70 Issue 3 Pages 235-246
    Published: August 25, 1959
    Released on J-STAGE: November 28, 2008
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  • Tatuo Saito, Tsutomu Yagi, Hiroshi Sasaki, Hisao Otsuka, Akiyuki Hoshi ...
    1959 Volume 70 Issue 3 Pages 247-260
    Published: August 25, 1959
    Released on J-STAGE: November 28, 2008
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  • Kaoru Yoshinaga
    1959 Volume 70 Issue 3 Pages 261-269
    Published: August 25, 1959
    Released on J-STAGE: November 28, 2008
    The method described by Weil-Malherbe and Bone for the estimation of the catecholamines in blood plasma was reinvestigated. The values estimated by this technique are unduly high because protein and possibly other contaminants are present in the eluate. When compared with THI method, the ED method gives significantly higher values than the former.
    Attempts were made to eliminate protein in the eluates and to make an extract blank by destroying the catecholamines in one aliquot of the sample, but both failed.
    The remarkable discrepancy between the catecholamine levels estimated by various authors using ED method is easily understood if it is assumed that the samples were purified in different grade, resulting in different degrees of contamination.
    The THI method may be excellent one, but it requires large amounts of blood for estimation, because it is not adequately sensitive. Thus the THI method is also unapplicable in clinical laboratories.
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  • Kitsuya Iwama, Chosaburo Yamamoto
    1959 Volume 70 Issue 3 Pages 271-280
    Published: August 25, 1959
    Released on J-STAGE: November 28, 2008
    A suppressing effect of γ-aminobutyric acid (GAB) upon cortically or reflexly induced movements was studied with cats by injecting the substance intracisternally in amount less than 10mg.
    1. Cortically induced movements due to strychninization or electrostimulation of the motor cortex were arrested by GAB within a short time. At this time there could not be found any noticeable changes of electrical activity in the motor cortex which was the impulse origin of the suppressed motor response.
    2. A suppression of spinal reflexes was obtained by administrating GAB. It was associated with a suppression of the cortically induced movement and spontaneous shivering. The reflex suppression was found to occur in decerebrate cats as well, when GAB was sprayed around the cut end of the brain stem.
    3. When the spinal cord was transected acutely, the reflex activity of the isolated spinal segments escaped the suppressing effect of GAB.
    4. It was found in some cases that EEG synchronization was caused by GAB in association with the reflex suppression.
    5. Concerning the site of action of GAB, a supposition was made that the motor suppression due to GAB might be the result of an inhibition of the reticular facilitatory system of the brain stem.
    The authors are indebted to Dr. Tomoaki Asano for his invaluable suggestions in regard to preparing the manuscript. A part of the expense for this work was defrayed by a grant of the Ministry of Education.
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  • Suteyo Yagi, Kiyoshi Sakurai, Hyoichiro Kusakari
    1959 Volume 70 Issue 3 Pages 281-290
    Published: August 25, 1959
    Released on J-STAGE: November 28, 2008
    1) The contents of cytochrome C and nucleic acids in the fresh muscle strips and the stored powders from various parts of the dog heart were determined.
    2) The left heart in situ contains probably 19 mg. % of cytochrome C, 8mg. % of DNA-P and 20mg. % of RNA-P.
    3) The puppie's hearts contain 1.4 times as much DNA and cyto-chrome C as that of adult dogs. The RNA contents are rapidly lost after death, and also are not differentiated between both groups.
    4) Among the various parts of the dog heart, both RNA and cytochrome C are concentrated predominantly in the left heart, which contains comparatively smaller amounts of DNA and then may consist of much larger cells. The auricles, which consumes oxygen most actively, contain the least amount of cytochrome C.
    5) During the maturation of the dog, the right heart of which the absolute content of cytochrome C is relatively small, synthesizes this enzyme increasingly, and in contrast the left heart apparently discontinues its synthesis.
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  • I. Clinical Observations
    Iwao Yamaguchi
    1959 Volume 70 Issue 3 Pages 291-304
    Published: August 25, 1959
    Released on J-STAGE: November 28, 2008
    Catalase activity of the liver was measured in 82 cases with gastric carcinoma as well as in 18 cases with peptic ulcer, resulting in the following conclusions.
    1. Liver catalase activity is generally lower in gastric carcinoma cases than in the controls.
    2. In gastric carcinoma cases, the lower catalase activities are observed in cases with the more progressed lesions, that is, in cases with the more extended disseminations and in those with the more widely involved lymph nodes, while the activities have little relation to the morphological features of the tumor themselves.
    3. In gastric carcinoma cases, the liver catalase activities are comparatively low in those with severe anemia and in those with hypoproteinemia.
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