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Volume 177 (1995)
- Issue 4 Pages 271-
- Issue 3 Pages 183-
- Issue 2 Pages 93-
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Volume 177, Issue 4
Displaying 1-11 of 11 articles from this issue
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KAZUAKI SHIMAMOTO, KATSUHIRO HIGASHIURA, MOTOYA NAKAGAWA, ATSUSHI MASU ...1995 Volume 177 Issue 4 Pages 271-278
Published: 1995
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSHIMAMOTO, K., HIGASHIURA, K., NAKAGAWA, M., MASUDA, A., SHIIKI, M., MIYAZAKI, Y., ISE, T., FUKUOKA, M., HIRATA, A. and IIMURA, O. Effects of Hyperinsulinemia under the Euglycemic Condition on Calcium and Phosphate Metabolism in Non-Obese Normotensive Subjects. Tohoku J. Exp. Med., 1995, 177 (4), 271-278 - The effect of acute insulin infusion on the metabolism of calcium (Ca) and phosphate (P) was examined in 17 healthy subjects. They were hospitalized and kept on a constant diet for 5 days, and an euglycemic hyperinsulinemic glucose clamp was applied. Synthetic human insulin was infused at the rate of 40 mU/m2/min for 2hr, and glucose was also infused to maintain basal glucose levels of each subject. The control study was performed in 8 of the 17 subjects, into whom 10% xylitol was infused for 2hr at the rate of 100ml/hr. The plasma insulin concentrations were 7.94±0.35 and 62.3±143mU/liter before and after the glucose clamp technique, but serum free Ca ion was increased significantly (p< 0.05), and serum P and serum parathyroid hormone (PTH) were decreased significantly (p<0.001). Creatinine clearance did not change during the glucose clamp technique. Urinary excretion of Ca (UCaV) was significantly higher after the glucose clamp than the control study. Fractional excretion of Ca (FECa) was increased significantly (p<0.05), and urinary excretion of P (UPV) and fractional excretion of P (FEP) were decreased significantly (p<0.05) under the hyperinsulinemic condition. The results suggested that, under the conditions of euglycemic hyperinsulinemia by glucose clamp technique, insulin increased the serum free Ca ion, and as a result, PTH was suppressed. Decreased PTH might induce calciuresis and enhance tubular P reabsorption under hyperinsulinemia. Insulin increased serum free Ca ion might relate to the vasodilating action of insulin by its decrease of intracellular free Ca ion in vascular smooth muscle. - hyperinsulinemia; glucose clamp technique; Ca metabolism; P metabolism; PTHView full abstractDownload PDF (854K) -
TAKASHI DOI, HIROO NAITO, MICHINAGA TAKAHASHI, CHIKASHI SHIBATA, IWAO ...1995 Volume 177 Issue 4 Pages 279-291
Published: 1995
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSDOI, T., NAITO, H., TAKAHASHI, M., SHIBATA, C., SASAKI, I. and MATSUNO, S. Effect of Chronic Pancreatic Juice Diversion on Enteroglucagon Secretion and Intestinal Mucosal Growth in Dogs. Tohoku J. Exp. Med., 1995, 177 (4), 279-291 - We developed a new canine model of chronic pancreatic juice diversion from duodenum to distal ileum in order to investigate the effect of pancreatic juice on the secretion of enteroglucagon, and the growth of intestinal mucosa. In seven adult mongrel dogs, the minor pancreatic duct was ligated and excised. Then, 3 cm of duodenal segment including the pancreatic duct was transformed into a small pouch which was anastomosed to the distal ileum. Butter ingestion tests were performed before and after surgery to evaluate the effect of surgery on enteroglucagon secretion. In addition, intestinal mucosal thickness was measured, using specimens taken both at the time of surgery and during autopsy at the end of the study. We observed significant hypersecretion of enteroglucagon and mucosal growth throughout the whole small intestine. However, extent or grade of mucosal growth was not related to the mucosal contact of pancreatic juice. These results suggested that chronic pancreatic juice diversion from duodenum to the distal ileum induced mucosal growth of the whole small intestine and hypersecretion of enteroglucagon may play a important role in this growth. - Pancreatic juice diversion; enteroglucagon; intestinal mucosal growth; pancreaticoileostomyView full abstractDownload PDF (2381K) -
HISAYA TADA, HITOSHI KAWAI, HIROYUKI ISHII, KAYOKO NOMURA, TSUTOMU URA ...1995 Volume 177 Issue 4 Pages 293-302
Published: 1995
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSTADA, H., KAWAI, H., ISHII, H., NOMURA, K., URAYAMA, T, and ISOGAI, S. Thrombospondin Modulates Adhesion, Proliferation and Production of Extracellular Matrix in Mesangial Cells. Tohoku J. Exp. Med., 1995, 177 (4), 293-302 - Thrombospondin (TSP) is produced by glomerular mesangial cells and one of the extracellular matrix in the mesangium, whereas the physiological role of TSP in mesangial cells is poorly understood. In order to know whether TSP modulates mesangial cell functions, we investigated the effects of TSP on cell adhesion, proliferation, synthesis of extracellular matrix and serine proteinases in cultured human mesangial cells. The substratum of TSP inhibited cell attachment and spreading in a TSP-dose-dependent manner in mesangial cells. Soluble TSP (50 μg/ml) also caused the detachment of fully adherent mesangial cells, whereas TSP less than 10μg/ml did not. [3H]-thymidine incorporation into mesangial cells was dose-dependently reduced by TSP. On the other hand, the production of both fibronectin and type IV collagen from mesangial cells was enhanced by TSP. The incubation of mesangial cells with TSP increased the secretion of tissue-type plasminogen activator (tPA) and urokinase-type plasminogen activator (uPA), while plasminogen activator inhibitor-type 1 (PAI-1) decreased. These observations indicate that TSP inhibits cell adhesion and proliferation in cultured human mesangial cells. It is also suggested that TSP influences the metabolism of mesangial matrix by modulating both synthesis and degradation of matrix components. Thus, TSP may be an important mediator of mesangial cell functions in an autocrine fashion. - thrombospondin; mesangial cells; cell adhesion; proliferation; extracellular matrixView full abstractDownload PDF (985K) -
TOKIHISA KIMURA, KOZO OTA, MASARU SHOJI, TAKEHARU FUNYU, MASAHIRO OHTA ...1995 Volume 177 Issue 4 Pages 303-313
Published: 1995
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKIMURA, T., OTA, K., SHOJI, M., FUNYU, T., OHTA, M., SATO, K., YAMAMOTO, T., MORI, T., SAHATA, T., SUGIMURA, K. and ABE, K. Chlorpropamide-Induced ADH Release, Hyponatremia and Central Pontine Myelinolysis in Diabetes Mellitus. Tohoku J. Exp. Med., 1995, 177 (4), 303-313 - Chlorpropamide (CPM) has been reported to produce impaired water excretion due to the enhancement of renal vasopressin (ADH) action and/or due to centrally enhanced ADH release, but it is still unknown whether CPM gives rise to ADH release with a subsequent hyponatremia in diabetes mellitus (DM), which, in turn, causes an impairment of the central nervous system. In 3 patients with DM, who developed hyponatremia during the treatment with CPM, an acute water load (WL) was carried out in the presence and absence of the drug, and plasma ADH was determined with plasma and urine osmolalities. Moreover, in 2 cases, MRI scans of the brain were taken. In all the patients, acute WL tests failed to suppress completely ADH release in response to changes in plasma osmolality in the presence of CPM, which, in turn, resulted in the impaired water excretion. In the absence of CPM, an acute WL normally suppressed plasma ADH leading to the diuresis. MRI scans illustrated the presence of central pontine myelinolysis. It is likely that CPM might stimulate ADH release in DM with a subsequent hyponatremia and brain damages. - SIADH; vasopressin; neuropathyView full abstractDownload PDF (1424K) -
SHUICHI MORI, WATARU WATANABE, SHIRO SHIGETA1995 Volume 177 Issue 4 Pages 315-325
Published: 1995
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSMORI, S., WATANABE, W. and SHIGETA, S. A Colorimetric LDH Assay for the Titration of Infectivity and the Evalution of Anti-Viral Activity against Ortho- and Paramyxoviruses. Tohoku J. Exp. Med., 1995, 177 (4), 315-325 - A rapid and precise screening assay was developed for in vitro evaluation of anti-orthomyxo- and anti-paramyxovirus agents. The procedure is spectrophotometrical assessment for viability of cells via extracellular leakage of lactic dehydrogenase (LDH). HMV-II cells, a human melanoma cell line was found to be suitable for the titration of virus infectivity and screening of anti-viral agents for orthomyxo- and paramyxoviruses. Comparative titration of infectivity of stock viruses by the LDH and the MTT in site reduction of 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide (MTT) methods with HMV-II cells as well as plaque titration with MDCK, Vero and HeLa cells was carried out. The LDH method was comparable or more sensitive for influenza viruses (FLUV)-A, B, C, parainfluenza viruses (PFLUV)-1, 2 and less sensitive for PFLUV-3, mumps virus (MPSV), measles viruses (MLSV) and respiratory syncytial virus (RSV) than the plaque titration. The 50% effective concentration (EC50) of 1-β-D-ribofuranosyl- 1, 2, 4-triazol-3-carboxamide (ribavirin) and 5-ethynyl-1-β-D-ribofuranosyl- imidazole-4-carboxamide (EICAR) against orthomyxo- and paramyxoviruses were examined comparatively by the LDH, MTT and plaque reduction (PR) methods. The EC50 values of FLUV-C and PFLUV-1 were able to be evaluated only by the LDH but not by the MTT and PR methods. The LDH method with HMV-II cells simplifies the assay procedure and permits the evaluation of a large number of compounds for anti-orthomyxo- and anti-paramyxoviruses activity in vitro. - orthomyxovirus; paramyxovirus; anti-viral assay; LDH assay; MTT assayView full abstractDownload PDF (1143K) -
AKIRA SHIMODA, KIYOSHI HANAUMI, KATSUO KUMAGAI1995 Volume 177 Issue 4 Pages 327-335
Published: 1995
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSHIMODA, A., HANAUMI, K. and KUMAGAI, K. TNF Inhibitor with a Low Molecular Weight Found in the Human Sera. Tohoku J. Exp. Med., 1995, 177 (4), 327-335 - We found a TNF inhibitory factor with a molecular weight of 5 to 10kDa in the human sera. The activity was detected by inhibiting the activity of serum to TNF-induced cytotoxicity against target cells. It was found in sera of all the healthy donors tested without any febrile diseases. Moreover, our results demonstrated that TNF inhibitory factor decreases in the semum of patients on regular hemodialysis treatment and in the serum of diabetes mellitus patients. The activity found in human sera was eluted from DEAE-cellulose column (Mono Q) at 0.25 and 0.45M NaCl, and was labile to incubation for 60min at 56°C and susceptible to treatment with trypsin, which destroyed 60% of its biological activity. TNF inhibitory factor may act as a regulator of the biological activity of TNF and could have beneficial effects in certain inflammatory conditions, and therefore, could be useful in clinical application. - TNF inhibitor; TNF inhibitory factor; cytotoxicity; human serumView full abstractDownload PDF (868K) -
YUKIHIRO TERADA, TAKAO FUKAYA, HIRONOBU HARAYA, AKIRA YAJIMA1995 Volume 177 Issue 4 Pages 337-341
Published: 1995
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSTERADA, Y., FUKAYA, T., HARAYA, H, and YAJIMA, A. Sperm Motility Characteristics and Pregnancy Outcome of Artificial Insemination with Husband's Semen for Male Infertility. Tohoku J. Exp. Med., 1995, 177 (4), 337-341 - What parameter of semen has influence on the pregnancy outcome of artificial insemination with the husband's semen (AIH) with washed sperm for male infertility was investigted. Two hundred fifteen cycles in 62 patients of AIH with washed sperm for male infertility were prospectively studied. We compared six parameters (total sperm count, motility rate, sperm motile efficiency (SME) both before and after washing) in pregnant and non-pregnant cycles. SME of washed sperm was significantly higher in the pregnant than in the non-pregnant cycles (241.0±68.0 vs. 169.5±80.0). There were no differences in other five parameters between pregnant and non pregnant cycles. SME, the parameter of sperm motility characteristics, of washed sperm is closely related to the outcome of AIH for male infertility. Sperm motility characteristics after washing may be one of the important indexes of the result of AIH for male infertility. - male infertility; artificial insemination with husband's semen (AIH); sperm motile efficiency (SME)View full abstractDownload PDF (510K) -
KATSUHISA IKEDA, TAKASHI SAKURADA, TOMONORI TAKASAKA, TAKUJI OKITSU, S ...1995 Volume 177 Issue 4 Pages 343-351
Published: 1995
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSIKEDA, K., SAKURADA, T., TAKASAKA, T., OKITSU, T, and YOSHIDA, S. Anosmia Following Head Trauma: Preliminary Study of Steroid Treatment. Tohoku J. Exp. Med., 1995, 177 (4), 343-351 - Twenty patients with posttraumatic anosmia were subjected to olfactory function testing, including olfactory acuity tests using a T & T olfactometer and an intravenous olfaction test. T & T tests revealed complete loss in 14 patients. In the intravenous olfaction test, 14 patients showed no response and 5 patients showed abnormal responses. The severity of olfactory dysfunction showed no correlation with background factors such as the site of head trauma, the presence of the fracture of skull, the presence of unconsciousness, or the presence of head operation. As a preliminary study, seventeen patients were administered a corticosteroid, a topical nasal drop of 0.1% betamethasone for 12 patients and an oral administration of prednisolone for 5 patients. Four patients showed slight recovery of olfactory function following a corticosteroid therapy. Effects of corticosteroids on olfaction might be explained by regeneration of olfactory receptor cell axons and reestablishment of contact with cells in the olfactory bulb. - anosmia; head trauma; corticosteroidView full abstractDownload PDF (721K) -
MIKIKO NAGAE, YASUHIRO AOKI, MASAYUKI NATA, MASAKI HASHIYADA, KAORU SA ...1995 Volume 177 Issue 4 Pages 353-364
Published: 1995
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNAGAE, M., AOKI, Y., NATA, M., HASHIYADA, M. and SAGISAKA, K. Monoclonal Antibody against an Amniotic Protein Carrying ABH Blood Group Epitopes and Its Forensic Application. Tohoku J. Exp. Med., 1995, 177 (4), 353-364 - A mouse monoclonal antibody against an amniotic protein carrying ABH antigenic epitopes was established. BALB/c mice were immunized by an amniotic protein of molecular weight over 200kDa, which had proved to be the carrier protein of ABH blood group epitopes by analysis with SDS-PAGE and immunoblotting. The antibody, ASP-1, was directed to the amniotic carrier protein without affecting the ABH blood group antigenicity, and did not cross-react with other body fluids which included blood, saliva, semen, urine or vaginal secretion. The immunoglobulin class of ASP-1 was IgG1 with a titer of 1:1, 600. ASP-1 was used to detect the ABH blood group of amniotic fluid by the sandwich ELISA in which wells of plates were coated with ASP-1, and the ABH blood group of the captured protein was detected with mouse IgM anti-A and -B antibodies and enzyme conjugated anti-mouse IgM. The sandwich ELISA could successfully detect the blood group of amniotic fluid in mixed body fluids. - amniotic fluid; ABH blood group; ELISA; monoclonal antibody; forensic serologyView full abstractDownload PDF (1500K) -
MASAKI AKIYAMA, HIDEKI TAKAMI, YUTAKA YOSHIDA1995 Volume 177 Issue 4 Pages 365-374
Published: 1995
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSAKIYAMA, M., TAKAMI, H. and YOSHIDA, Y. The Mechanism of Cold-Induced Platelet Aggregation in the Presence of Heparin. Tohoku J. Exp. Med., 1995, 177 (4), 365-374 - Low temperature induces platelet aggregation, but this phenomenon is slight and poorly reproduced. However, heparin potentiated the reaction in a dose dependent manner. The degree of aggregation increased as the temperature at which the platelet-rich plasma was chilled was lowered, and as the time of chilling lengthened. Acetylsalicylic acid, a cyclooxygenase inhibitor, and staurosporin, an inhibitor of protein kinase C, partially inhibited cold-induced platelet aggregation (CIPA), suggesting that at least part of the reaction mechanism involves production of thromboxane A2 and activation of protein kinase C. Prostaglandin E1 (PGE1), which inhibits platelet responses through elevating platelet cyclic AMP, completely blocked CIPA, suggesting that PGE1 dependent pathway in platelets plays an important role for CIPA. The inhibition of CIPA by these inhibitors suggests that CIPA is aggregation with platelet activation but not platelet agglutination. Extracellular Ca2+ is essential for CIPA because ethylene glycol-bis (β-aminoethylether) N, N, N', N'-tetraacetic acid (EGTA), extracellular
Ca2+ chelating agent, completely inhibited CIPA. Monoclonal antibodies against glycoprotein (GP) IIb/IIIa (10E5, P2) and Ang-Gly-Asp-Ser-peptide (RGDS-peptide) which inhibit fibrinogen binding to GPIIb/IIIa completely blocked CIPA but monoclonal antibodies against GPIb (6D1, SZ2) partially. In addition, CIPA occurred only when fibrinogen was added to washed platelets suspension. These results indicate that CIPA is dependent on binding of fibrinogen to GPIIb/IIIa and GPIb is partly related with the reaction. - platelet; aggregation; temperature; heparinView full abstractDownload PDF (835K) -
YASUO YAMADA, HIDEMI YAMAUCHI, KENJI KAKIZAKI, HIROYOSHI SUZUKI1995 Volume 177 Issue 4 Pages 375-377
Published: 1995
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSYAMADA, Y., YAMAUCHI, H., KAKIZAKI, K. and SUZUKI, H. Expression of Ki-67 by Monoclonal Antibody MIB-1 in Carcinoma of the Middle and Lower Bile Duct. Tohoku J. Exp. Med., 1995, 177 (4), 375-377 - To evaluate the relationship between cell proliferation and clinical outcome of bile duct carcinoma, MIB-1 immunohistochemistry and clinicopathological study were performed in 21 patients who underwent resection of carcinoma of the middle and lower bile duct. Some histological factors, especially pancreatic infiltration affected survivals. MIB-1 scores (percentages of MIB-1 positive cells) tended to be higher in the patients who died within three years after operations than in the group that survived over three years. No relationship was observed between MIB-1 scores and histological tumor spread. These results suggest that MIB-1 scores may be useful to predict the clinical outcome of the patients with carcinoma of the bile duct, independently of histological tumor spread. - carcinoma of the bile duct; immunohistochemistry; Ki-67; MIB-1; survival rateView full abstractDownload PDF (341K)
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