1. Group A and O lipids were separated in an electrophoretically homogeneous state from human lungs of individuals belonging to respective blood groups, although group specificity became a little ambiguous during the course of preparation in accord with the group conversion
in vitro of the pig lung group lipid which had been discovered by Masamune and Akama.
2. a) The preparations showed no discrepancies of solubility and N, P and galactose contents between Groups A and O.
b) The Group A and O lipids both contained aspartic and glutamic acid, serine, glycine, arginine, valine and/or methionine and phenylalanine as amino acids, and the hexosamines and galactose as sugars. Choline, glycerophosphoric acid, a fatty acid or acids and a higher alcohol or alcohols were also detected in the both group lipids.
c) Either of those group lipids was devoid of mannose, L-fucose, inositolphosphoric acid, plasmal and sphingosine.
In short, no chemical difference was found between these Group A and O lipids in so far as examined.
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