The effects of droperidol combined with pentazocine and with fentanyl on the pulmonary hemodynamics were investigated in intact dogs anesthetized with urethane-chloralose, and the results were compared with those of each analgesic alone. Mean pulmonary arterial pressure and pulmonary driving pressure remained almost unchanged after droperidol and pentazocine. Pulmonary vascular resistance decreased significantly, and pulmonary vascular compliance and its radius tended to increase. These results indicate that droperidol counteracts the vasoconstrictive action of pentazocine on the pulmonary hemodynamics. On the contrary, almost, all the parameters of the pulmonary hemodynamics after droperidol and fentanyl changed to similar directions to those after fentanyl alone. Droperidol seemed not to modify but to exaggerate the pulmonary circulatory effects of fentanyl.
The common bile ducts of the Wistar rats were ligated and severed, and liver biopsies were done weekly for 7 postoperative weeks. Light and electron microscopic specimens were prepared for the morphometric studies. The volume ratio of the hepatic parenchyma declined with the lapse of time after bile duct ligation. However, elevated mean sectional area of the nucleus, increased mitotic index and unchanged estimated weight of the hepatic parenchyma after biliary obstruction suggested that lost hepatocytes were compensated by regeneration. Mitochondrial swelling and curling of the cristae were noted in biliary obstruction in general. Moreover, both the number and volume ratio of the mitochondria were increased corresponding to the duration of biliary obstruction. These changes were interpreted as an adaptation process to mito-chondrial dysfunction.
In 87 cases of acute leukemia, leukemic and normal hematopoietic cell count in the bone marrow was serially observed, and the findings were used for evaluating the effectiveness of antileukemic agents and also for determining the grade of decrease in the marrow leukemic cell count required to start the proliferation of normal hematopoietic cells and to obtain complete remission of acute leukemia in adults.
The effects of anticancer agents were studied on the basis of survival rates following surgery for gastric carcinomas in different histologic types of Lauren's classification. It was revealed that Lauren's classification would provide an index to the use of anticancer agents to a certain extent. Anticancer agents were effective against diffuse gastric carcinoma, and should therefore be intensively used for treatment of this malignancy. For female patients with diffuse gastric carcinoma, careful selection of adequate anticancer agents is highly necessary. Anticancer agents were less effective against intestinal-type carcinoma, and it would therefore be necessary to use anticancer agents on the aged male patient with this malignancy by taking his age and side effects of the agents into consideration.
Distal two-thirds of the esophagus without macroscopically detectable malignant lesions from a total of 248 autopsy cases were examined for epithelial dysplasia on histological sections from serial blocks. The autopsy specimens had been obtained from Miyagi, Nara and Wakayama prefectures known for high incidence of esophageal carcinoma in Japan, and Aomori prefecture where the incidence of the disease is low. Epithelial dysplasia was classified into mild, moderate, and severe including carcinoma in situ according to the grade of epithelial atypism. Of 248 cases 91 (36.7%) had epithelial dysplasia and 30 (12.1%) had moderate and severe dysplasia. In one of the cases of severe dysplasia, in situ carcinoma was diagnosed. Lesions of dysplasia of the resected specimen were displayed in a diagram for the distribution of the abnormal epithelium. Cases of higher grade dysplasia tended more extensive in area and were slightly dominant in the distal third of the esophagus. Possible relationships of dysplasia with long-standing irritation to the esophagus and with precancerous lesions were discussed.
Chromosome studies of an infant with multiple malformations were made by means of the trypsin-Giemsa banding as well as conventional Giemsa staining methods. The propositus showed 46, XY, +3, -C, and it was indicated that the abnormal metacentric chromosome was induced by the pericentric inversion of chromosome No. 8, in which chromosomal breakage had occurred most likely at the bands Sp23 and 8g23. The probable formula of the inversion is 46, XY, inv (8) (p23q23). Karyotypic analyses of the parents revealed no abnormalities, and the inversion therefore occurred spontaneously. Clinical features of the propositus are postulated to be caused by a loss of very small portions of the chromosomal material with the occurrence of the pericentric in-version.
HbA1 weight in individual red cells, amounting to 13, 746, was quantitatively determined in 72 male and 17 female healthy adults. The red cells were besmeared on the anti-HbA1 serum agarose plate and the precipitation rings formed were measured from the size printed on sheets of printing paper. HbA1weight in individual cells was calculated from the calibration curve prepared specially for HbA1 weight in a red cell and the volume of a precipitation ring. HbA1 weight in individual cells ranged from 4.7 to 62.6 pg and averaged 23.7 pg. The mean HbA1 weight in individual cells per head ranged from 18.1 to 34.0 pg and averaged 26.4±4.3 pg. No difference in the mean HbA1 weight was found between male and female adults, and among the age groups of them. Average ratio of the mean HbA1 weight in individual cells to the total hemoglobin in the average red cell was 87.4%. The HbA1 weight in the average red cell was determined from HbA1 concentration and red cell counts of the whole blood. The correlation coefficient between the mean HbA1 weight in individual cells and the HbA1 weight in the average red cell was 0.546 (p<0.001).
Making use of common antigenicity among allogeneic tumors, the immunotherapy against primary 3-methylcholanthrene(MCA). induced sarcomas in rats was attempted. At the time when MCA-induced sarcomas reached approximately 1cm in diameter, allogeneic Usubuchi sarcoma cells were injected 5 times into the subcutaneous tissue of rats. In most cases, inhibitory effect on the growth of the tumor was observed in the early period, but none of 29 cases treated resulted in final regress-ion. Twenty-nine non-treated cases showed uniformly progressive growth from the beginning. This result seems to indicate the possibility of using allogeneic tumor(s) for the immunotherapy against human cancer.
This study was conducted under a working hypothesis that the repeating of selective breeding of normal rats with a slight impairment of glucose tolerance would produce spontaneous diabetes rats. This is the second report. 18 rats were selected by an oral glucose tolerance test from 211 normal Wistar rats obtained from an experimental animal farm. By the breeding 162 Fl rats were obtained. By the selective breeding in this way, 204 F2, 174 F3 and 215 F4 rats were obtained. The mean glucose tolerance curve became more diabetic with the increasing number of generations. The percentage of cases of a positive test for urine sugar during the glucose tolerance test increases with the number of generation. The results indicate the possibility of production of spontaneous diabetes from normal rats by the repetition of the selective breeding.
In non-obese but diabetic 15-week-old KK mice which showed fatty liver histopathologically, the content of liver lipids and the levels of blood glucose and plasma IRI were greater than those in the control ICR mice of the same age and were quite similar to those in GTG-obese mice. In 6-week-old KK mice which excreted no glycosuria and showed normal hepatic tissues, only plasma IRI level was slightly elevated as compared with that in the control mice. The cyclic 3', 5'-AMP stimulators like epinephrine and theophylline exerted far less potent stimulatory effects on lipolytic activity in 6-week-old KK mice than in the control mice, as in diabetic 15-week-old KK mice and GTG-obese mice. Theophyl-line potentiated the lipolytic effect of epinephrine linearly in KK mice, the tendency being different from that in the control mice, and only the submaximal rate was obtained. Furthermore, the inhibitory effect of theophylline on PDE from the epididymal adipose tissue was less potent in 6-week-old KK mice than in healthy ICR mice of the same age.
The effects of potassium chloride on inotropic and chronotropic activity were investigated in five isolated canine atrium preparations which were suspended in a bath and perfused with arterial blood from the carotid artery of the heparinized support dog. Potassium chloride administered into the cannulated sinus node artery in a dose range of 100 μg-1 mg produced a dose-related negative inotropic and a positive chronotropic effect. These effects were not influenced by treatment with either atropine or propranolol. From these results, it is concluded that potassium has a direct negative effect on atrial contractility and a direct positive effect on atrial rate.