TAKEDA, T. and NAGAI, B. NH4Cl-HCO3--Mediated Hemolytic Reac tion in theErythrocytes of Hereditary Spherocytosis. Tohoku J. exp. Med., 1986, 148 (2), 119- 123 - A NH4Cl-HCO3--mediated, acetazolamide modulated hemolysis test was carried out in seven patients with hereditary spherocytosis (HS), and the amount of enzyme antigen as well as activities of carbonic anhydrase (CA) were determined. Except for a decrease in CA-B activity (p<0.05), the amount of enzyme antigen, total and specific activities of both isozymes of CA and dependent activity of CA-C were within the normal range. Fifty percent and 95% hemolysis times were significantly shorter than the normal range, and they remained abnormal even in the presence of acetazolamide. This hemolysis test, especially H50 valuse, was therefore shown to have diagnostic value in HS.
OIKAWA, N., UMETSU, M., TOYOTA, T. and GOTO, Y. Quantitative Evaluationof Diabetic Autonomic Neuropathy by Using Heart Rate Variations: Relationshipbetween Cardiac Parasympathetic or Sympathetic Damage and Clinical Conditions. Tohoku J. exp. Med., 1986, 148 (2), 125-133 - It was previously suggested that respiratory heart rate (HR) variations were predominantly affected by the parasympathetic nerve and HR increase to standing was predominantly affected by the sympathetic nerve. To compare parasympathetic and sympathetic nerve function in diabetics, these two tests were performed in 95 diabetics and 38 controls by use of an instantaneous-HR-change continuous recorder. All subjects were between 40 and 59 years-old. As indices of autonomic nerve function, the mean difference between maximal and minimal HR during deep breathing (ΔI-E) and the HR increase on standing (ΔHR) were determined. The mean ΔI-E and ΔHR in the diabetics were 9.4 beats/min and 15.1 beats/mm, respectively. These values were significantly lower than those in the controls (ΔI-E 14.4, ΔHR 20.5 beats/min). ΔI-E correlated negatively to duration of diabetes and mean fasting blood glucose during the last 6 months in the diabetics, but ΔHR did not correlate to them at all. Diminished ΔI-E was found in the patients with insulin treatment, retinopathy or persistent proteinuria. Diminished ΔHR, however, was found only in the patients with long-standing complicated diabetes. Thus, both cardiac parasympathetic and sympathetic nerve function were significantly impaired in the diabetics as compared with the controls and that parasympathetic nerve damage occurred early whereas sympathetic innervation was preserved.
YUMITA, S., FURUKAWA, Y., SOHN, H.E., UNAKAMI, H., MIURA, R. and YOSHINAGA, K. Familial Idiopathic Hypoparathyroidism and ProgressiveSensorineural Deafness. Tohoku J. exp. Med., 1986, 148 (2), 135-141 - Three cases from two families with idiopathic hypoparathyroidism and progressive sensorineural deafness are described. Cases 1 and 2 were siblings. Case 3 was one of four siblings from another family. All of them had both idiopathic hypoparathyroidism and progressive sensorineural hearing loss. There was no evidence to suggest involvement of autoimmune mechanism in these cases except for the associated Graves' hyperthyroidism in case 3. Human leukocyte antigen A9 and A11 were positive in both families. The sensorineural hearing loss was progressive even after the treatment for hypoparathyroidism. As the familial idiopathic hypoparathyroidism is a very rare entity, it is unlikely that this disease is associated with familial progressive sensorineural deafness by chance. The combination of these two diseases may compose a new syndrome.
KAWAI, H., SAKUMA, T., SASAKI, K., SATO, M., WATANABE, T., HONDA, M., OZAKI, S. and AZUMA, M. Application of Immune Adherence HemagglutinationTest in the Screening of Blood Donors Carrying a High Titer Antibody toVaricella-Zoster Virus. Tohoku J. exp. Med., 1986, 148 (2), 143-149 - The immune adherence hemagglutination (IAHA) test was compared with the complement fixation (CF) test and fluorescent antibody (FA) test to measure the antibody titer to varicella-zoster virus (VZV) in 70 sera of convalescents 2 to 8 weeks afte rherpes zoster onset. The IAHA titer correlated with the FA titer, but not with the CF titer which was lower than the IAHA and FA titers. Then, the IAHA test was used for screening of blood donors with a high titer (higher than 64 units) of antibody to VZV from 2, 592 blood donors. About 14% of the sera from the donors aged 16 to 19 years had an antibody titer higher than 64 units, about 10% of sera from the donors aged 20 to 49 years, and 5.6% of sera from the donors older than 50 years. An average positive rate was about 9%, indicating that an enough VZV immune plasma with a high titer of antibody might be obtained from blood donors.
HANEW, K., SATO, S., SASAKI, A., GOH, M., SHIMIZU, Y. and YOSHINAGA, K. The Dopaminergic Regulation of Plasma Growth Hormone Secretion in NormalSubjects. Tohoku J. exp. Med., 1986, 148 (2), 151-158 - The role of endogenous dopamine (DA) on plasma GH secretion was studied using domperidone (DA receptor blocker which does not cross blood brain barrier) in 16 normal subjects. After a bolus injection of domperidone (10mg, iv.), plasma PRL in 11 cases rose quickly and markedly from the basal level of 9.5±1.2ng/ml (Mean±S.E.) to a maximum of 76.3±10.6ng/ml at 30min. In contrast, plasma GH in these cases showed a delayed and slight increases to domperidone injection where the values at 90min and 120min (3.5±0.8ng/ml and 3.7±1.0ng/ml) were significantly higher than those in control study (1.2±0.2ng/ml and 1.0±0.1ng/ml ; p<0.05; n=8). Domperidone infusion (0.22mg/min/3hr) was performed in the remaining 5 subjects. The plasma PRL responses were similar to those in the bolus injection of domperidone. These PRL responses were not modified when L-dopa was administered 30min after the start of iv infusion of domperidone. Plasma GH showed slight but significant increases 135min after the infusion compared to control study (4.3±1.2ng/ml vs. 1.0±0.1ng/ml; p<0.05). By the prior infusion of domperidone plasma GH responses to L-dopa was delayed and blunted, i. e., the occurrence of elevation and peak value of GH delayed by 15min, and the values at 135min and 150min (2.3±0.5ng/ml and 1.5±0.2ng/ml) were significantly lower compared to those in the single L-dopa test (6.3±1.2ng/ml and 5.0±1.2ng/ml ; p<0.02 and 0.05, respectively). These results indicate that in normal subjects L-dopa has stimulatory role on GH secretion mainly at the level of CNS (hypothalamus) and partly at the level of median eminence. It is not plausible that endogenous DA has direct tonic inhibitory effects on pituitary somatotrophs.
NAKAMURA, R. and KOSAKA, K. Effect of Proprioceptive NeuromuscularFacilitation on EEG Activation Induced by Facilitating Position in Patients withSpinocerebellar Degeneration. Tohoku J. exp. Med., 1986, 148 (2), 159-161 - The shift of mean power in alpha band of EEG due to postural changes was examined in five patients with spinocerebellar degeneration, using EEG topography, before and after proprioceptive neuromuscular facilitation (PNF) maneuver. Normal EEG response to postural changes, i.e., an increase in the mean power at facilitating position compared to neutral position, was observed in only one patient before PNF maneuver, but in four after PNF maneuver. The improvement of motor function by PNF-treatment was clinically noticed in the patients with normal EEG response to postural changes after PNF maneuver.
ISAWA, T., TESHIMA, T., HIRANO, T., EBINA, A. and KONNO, K. Estimation ofa Drug Effect by a Simplified Radioaerosol Inhalation Lung Cine-Scintigraphy: Bromhexine on Mucociliary Clearance Mechanisms. Tohoku J. exp. Med., 1986, 148 (2), 163-167 - A proposed simplification of radioaerosol inhalation lung cine-scintigraphy, namely 60min measurement of radioactivity without repeating measurement at 24hr later and calculation of alveolar deposition ratio (ALDR) by the regression formula of ALDR=-48.08+0.47×FEV1.0%+0.59×LRR60, was found useful and applicable even to the evaluation of a drug on mucociliary clearance mechanisms. The simplified method indicated a very similar evaluation of the effect of bromhexine on airway clearance efficiency with those actually revealed by the original method.
ISAWA, T., TESHIMA, T., HIRANO, T., EBINA, A. and KONNO, K. MucociliaryClearance Mechanism in Interstitial Lung Disease. Tohoku J. exp. Med., 1986, 148 (2), 169-178 - To evaluate mucociliary clearance mechanisms of the lungs, 14 patients with pulmonary interstitial fibrosis and 8 with sarcoidosis were studied by lung function tests and radioaerosol inhalation lung cine-scintigraphy. Although all these patients showed interstitial densities on chest x-rays, only the patients with pulmonary interstitial fibrosis indicated restrictive and diffusion abnormalities by lung function tests and those with sarcoidosis did not show either of these functional abnormalities. Mucociliary clearance mechanisms were well maintained qualitatively and quantitatively in these patients with interstitial lung disease.
KOBARI, M., MATSUNO, S., YAMAUCHI, H., SATO, T., KUDO, T. and TACHIBANA, T. Human Pancreatic Cancer Associated Antigen Detected by Monoclonal Antibody. Tohoku J. exp. Med., 1986, 148 (2), 179-195 - Monoclonal antibody F30 was produced by the fusion of murine myeloma cell line P3-X63-Ag8-653 with spleen cells from a BALB/C mouse immunized with established human pancreatic cancer cell line (PK-1) and the reaction specificity was analyzed. The antigen recognized by monoclonal antibody F30 was different from HLA-associated antigen, β2-microglobulin, fetal bovine serum components, ferritin, AFP, or CEA. Monoclonal antibody F30 reacted with all of six pancreatic cancer cell lines established in our laboratory. Cross-reactivity was detected with a colon cancer cell line or an esophagus cancer cell line among various tumor cell lines tested. No reaction was detected with red blood cells, lymphocytes, or lymphoid and myeloid cell lines. By immunoperoxidase staining of frozen sections, the F30-defined antigen was detected not only on pancreatic cancer cell membrane but also on other adenocarcinomas. In addition, the monoclonal antibody F30 had a more widespread distribution on normal epithelial cells in the gastrointestinal organs, respiratory system, and urinary system. F30-defined antigen was composed of two protein components with molecular weight of 190 and 160K. It was indicated that the antigen was an integral protein in the cell membrane since the antigen was not detected in the spent culture medium of antigen-positive cells.
YAMAUCHI, H., SUNAMURA, M., TAKEDA, K., SUZUKI, T., ITOH, K. and MIYAGAWA, K. Hyperlipidemia and Pregnancy Associated Pancreatitis withReference to Plasma Exchange as a Therapeutic Intervention. Tohoku J. exp. Med., 1986, 148 (2), 197-205 - A 23 year old pregnant woman presented in her third trimester with severe pancreatitis and hyperlipidemia. Initial investigations suggested that her pancreatitis was induced by profound hypertriglyceridemia, which was the result of an underlying Fredrickson's V type hyperlipoproteinemia exacerbated by pregnancy. Concern for the life of the fetus prompted a caesarean operation and then drainage procedure for pancreatitis. Plasma exchange, carried out to lower the levels of lipids and the pancreatic enzymes, improved the signs and symptoms of the patient. Plasma exchange may be of great use in the management of hyperlipidemic pancreatitis.
IKEDA, M., WATANABE, T., HAYASHI, H. and TSUNODA, A. ToxicologicalEvaluation of Quartz and Silica Contents in Studded Tire-Generated Dust in theCity of Sendai. Tohoku J. exp. Med., 1986, 148 (2), 207-211 - Roadside dust samples were collected at six locations in the City of Sendai at the end of the winter season of 1980-1981, and analyzed for quartz and silica contents. X-ray diffraction and ICP analyses revealed that the mean quartz and silica contents in dust samples were 14.6% (ranging from 12.2 to 19.4%) and 56.3% (53.7 to 59.7%), respectively. From these results in connection with current dust levels and estimated duration of exposure, it appears likely that the risk of silicosis among the citizens may not be high if appropriate measures are taken to protect the atmosphere.
OTA, H., WAKIZAKA, A. and FUKUSHIMA, M. Modulation of Ovarian LHReceptor and Serum Hormone Levels in Rats with Hyperprolactinemia Induced byAdministration of Ovine Prolactin or Sulpiride. Tohoku J. exp. Med., 1986, 148 (2), 213-227 - Hyperprolactinemia was experimentally produced in rats by administration of ovine prolactin (oPRL) and sulpiride, and tried to evaluate the effect of hyperprolactinemia on ovarian receptor for luteinizing hormone (LH) as well as that on serum gonadotropin and steroid hormone levels. Wistar-Imamichi strain mature female rats showing 4-day estrous cycles were treated with various doses of oPRL or sulpiride twice a day for 4 days from diestrus. They were killed on the fifth day. Binding of ovarian LH receptors was reduced by a small dose of oPRL (0.1 IU) or sulpiride (0.25mg) and restored to normal by larger doses of oPRL. However, larger doses of sulpiride (50 or 100mg) increased the receptor bindings beyond the control level (4.39±0.40ng/mg homogenate protein). Serum prolactin levels decreased in rats treated with larger doses of oPRL, but increased with larger doses of sulpiride. Serum LH levels increased with both agents. Although the ovaries treated with either oPRL or sulpiride suggested the lack of ovulation, there were no significant changes of steroid hormones in oPRL groups. In contrast, sulpiride treatment resulted in a reduction of estradiol and an increase of progesterone secretion, suggesting the prolonged effect of the drug. Thus, prolactin appeared to act on the rat ovarian LH receptors in two different manners in hyperprolactinemia, depending on the amount of this hormone or a ratio of prolactin to LH.
YAMAMOTO, T., TAZAKI, M. and SAKADA, S. Morphological Study of theSensory Innervation of the Rat Labial Mucosa. Tohoku J. exp. Med., 1986, 148 (2), 229-238 - The sensory innervation of the rat labial mucosa was investigated by means of methylene blue vital staining and osmic acid staining. Sensory receptors in this region were of three kinds (free nerve endings, encapsulated corpuscles and bush-like nerve endings) which constituted separate sensory units respectively. The encapsulated corpuscles were observed in the deep part of lamina propria, and distributed mainly in the margin of labial mucosa. Almost all (78.8%) of encapsulated corpuscles were of a simple type which had a non-branched axon terminal. No clew-like type corpuscles or glomerular-Meissner corpuscles were observed. The bush-like nerve endings were located in the lamina propria close to the epithelium, and localized in the central part of labial mucosa where the formation of papillae was remarkable. The density of the encapsulated corpuscles in the entire mucosa was 3.5-5.3/mm2, and that of the bush-like nerve endings in the densely distributed area was 38.9-60.6/mm2
KODAMA, M., TSUBURAYA, Y., KOYAMA, H., KOTANAGI, H., ISHIKAWA, K., NARISAWA, T., KOYAMA, K., YAMAGUCHI, T. and TAKAHASHI, T. Production ofGroup I Pepsinogen by Gastric Carcinoma. Tohoku J. exp. Med., 1986, 148 (2), 239-240 - Group I pepsinogen levels were determined in the homogenized supernatant of the lymph nodes with metastasis of the gastric carcinoma by radioimmunoassay. Mean levels of group I pepsinogen were 941ng/g and some of the lymph nodes showed very high levels compared with those of non-metastatic lymph nodes, 269ng/g. They were also significantly higher than those of the sera in the same sabjects, that is, the production of group I pepsinogen by gastric carcinoma was clarified.