The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
Volume 135 , Issue 4
Showing 1-16 articles out of 16 articles from the selected issue
  • MASATOSHI OHTAKE, RYO CHIBA, KEIKO MOCHIZUKI, KEIYA TADA
    1981 Volume 135 Issue 4 Pages 335-343
    Published: 1981
    Released: November 17, 2010
    JOURNALS FREE ACCESS
    RTARE, M., CHIBA, R., MOCHIMICI, K. and TADA, K. Zinc and Copper Concentrations in Human Milk and in Serum from Exclusively-Breast-Fed Infants during the First 3 Months of Life. Tohoku J. exp. Med., 1981, 135 (4), 335-343-Zinc and copper concentrations in human milk and in serum from exclusively-breast-fed infants born at full-term were serially determined during the first 3 months of life by the atomic absorption spectrophotometoric method. Zinc and copper concentrations of human milk decreased as the stage of lactation progressed. The mean value of the concentration of serum zinc at birth was just below the value previously reported for school children, significantly decreased at one month of age (p<0.02), then returned to the level at birth at 2 months of age, and reached at 3 months of age to the level of school children. On the other hand, the mean value of serum copper at birth was markedly low, and rapidly increased until 2 months of age and then gradually increased until 3 months of age. These changes in serum zinc and copper concentrations during early infancy suggested that human milk can provide sufficient zinc and copper for full-term infants during the first 3 months of life. Calculated daily zinc and copper intakes in infants fed on human milk were lower than recommended values. It is necessary to reconsider the appropriateness of previously recommended values for breast-fed infants.-zinc; copper; human milk; serum; infants
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  • TETSU YAMAMOTO, SANYA SAKADA
    1981 Volume 135 Issue 4 Pages 345-347
    Published: 1981
    Released: November 17, 2010
    JOURNALS FREE ACCESS
    YAMAMOTO, T. and SAKADA, S. Distribution of Sensory Nerve Endings in the Labial Mucosa of the Mouse. Tohoku J. exp. Med., 1981, 135 (4), 345-347 The distribution of sensory nerve endings in the labial mucosa of the mouse was investigated by the vital methylene blue staining method. Encapsulated corpuscles were distributed uniformly throughout the mucosa, whereas bush-like nerve endings were localized in the area along the median line where the mucosal epithelium showed a considerable thickness. The number of the encapsulated corpuscles per individual was 72-133, and that of the bush-like nerve endings was 131-248.-sensory nerve endings; oral mucosa; lip; mouse
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  • YASUYUKI ENDO, SHIGEO MAMIYA, MAKI SATOH, KEIKO TAKAHASHI, TADASHI HAR ...
    1981 Volume 135 Issue 4 Pages 349-358
    Published: 1981
    Released: November 17, 2010
    JOURNALS FREE ACCESS
    ENDO, Y., MAMIYA, S., SATOH, M., TAKAHASHI, K. and HARADA, T. Plasma β-Thramboglobulin and Platelet Factor 4 in Patients with Chronic Ren al Failure and Effect of Hemodialysis. Tohoku J. exp. Med., 1981, 135 (4), 349-358-Significantly increased levels of plasma β-thromboglobulin (β-TG)(76.8±25.5ng/ml, p<0.01) were observed in 24 patients with chronic renal failure (blood urea nitrogen (BUN)> 20mg/100ml), as compared with normal subjects (13.2±5.6 ng/ml). The increase in β-TG was highly correlated with BUN (γ=0.651, p<0.01), creatinine (γ=0.778, p<0.01) and creatinine clearance (τ=-0.723, p<0.01). Plasma platelet factor 4 (PF4)(normal 5.0±2.0ng/ml) also increased significantly to 8.5±3.4ng/ml (p<0.01). However, statistical correlation between β-TG and PF4 was not found in these patients. The reason is thought to be due to differences in molecular weight (PF4 8, 000 MW; β-TG 36, 000 MW) and half-life time (PF4 30 min; β-TG 100 min), and due to the difficulty in calculating statistically the correlation becasue of the narrow distribution of PF4 levels. The high levels of β-TG (89.4±3.4ng/ml) showed a further increase (109.4±5.8ng/100 ml, p<0.01) after dialysis. This is thought to be due to hemoconcentration, because other blood factors such as RBC, WBC, platelets, fibrinogen, etc were elevated by about 20% during hemodialysis and because no adhesion of platelets to the cellulose membrane did occur. The increase in PF4, levels at 15 min (55.2±19.6ng/ml, p<0.01) and 1 hr (23.7±8.4ng/ml, p<0.01) of hemodialysis from the level before it (7.7±1.3ng/ml) is thought to be caused the effect of heparin infusion. The change in PF4 was not accompanied by the change in β-TG. During hemodialysis the decrease of other platelet functions such as adhesiveness, aggregation induced by ADP, collagen and PF3 remained unchanged.-chronic renal failure; β-thromboglobulin; platelet factor 4; hemodialysis
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  • JUN KUSAKARI, TOSHIMITSU KOBAYASHI, MASAAKI ROKUGO, EIICHI ARAKAWA, KE ...
    1981 Volume 135 Issue 4 Pages 359-369
    Published: 1981
    Released: November 17, 2010
    JOURNALS FREE ACCESS
    KUSAKARI, J., KOBAYASHI, T., ROKUGO, M., ARAKAWA, E., OHYAMA, K., KAWAMOTO, K. and SERINO, H. The Inner Ear Dysfunction in Hemodialyeis Patients. Tohoku J. exp. Med., 1981, 135 (4), 359-369-Hearing and vestibular tests were performed in 229 patients who were treated with hemodialysis (H. D.) and the following results were obtained.(1) One hundred and six patients exhibited bilateral hearing loss and 31 showed unilaterally impaired hearing.(2) Vestibular dysfunction was found in 83 patients.(3) Those who were impaired both in the cochlea and the vestibular organ were 60.(4) No correlations were observed between the occurrence rate of the inner ear dysfunction and Hct, BUN and serum creatinine levels.(5) The results in the present study did not suggest the possibility that the hearing loss was progressively getting worse during the period of H. D. therapy. Based on these results, the possible etiological factors in the inner ear dysfunction were discussed.-hemodialysis; inner ear dysfunction.
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  • KENZO KOBAYASHI, TETSUO ARAKAWA, HAJIME NAKAMURA, SHINJI CHONO, HIROAK ...
    1981 Volume 135 Issue 4 Pages 371-378
    Published: 1981
    Released: November 17, 2010
    JOURNALS FREE ACCESS
    KOBAYASHI, K., ARAXAWA, T., NAKAMURA, H., CHONO, S., YAMADA, H. and YAMAMOTO, S. Determination of Prostaglandins and the Effect of Indomethacin on Rat Gastric Mucosa. Tohoku J. exp. Med., 1981, 135 (4), 371-378-Prostaglandins protect the gastrointestinal mucosa against various ulcerogens. There are only a few reports about endogenous prostaglandins in the gastric mucosa. We present a method for determining mucosal prostaglandins and an investigation on the effect of indomethacin on prostaglandin E2 levels in the rat gastric mucosa. The levels of prostaglandins B, E1, E2, and F were higher in the antral mucosa (311±123, 632±147, 2099±455, and 415±115 ng/g, respectively) than in the fundic mucosa (33±2, 202±38, 914±229, and 138±22 ng/g, respectively). In both mucosal tissues, the prostaglandin E, level was the highest and it was markedly reduced with indomethacin in a dose-related manner. The difference in the mucosal levels of prostaglandins suggests that in rats, mucosal damage occurs mainly in the fundus. The observed marked reduction of mucosal prostaglandin E2 following the administration of indomethacin may aid in elucidating the pathogenesis of gastric mucosal lesions induced by these types of agents. mucosal prostaglandin E2; indomethacin; cytoprotection; gastric mucosal lesions; radioimmunoassay
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  • SHIGERU TSUCHIYA, SHINGI NAKAE, TASUKE KONNO, KEIYA TADA, YASUSHI ONO
    1981 Volume 135 Issue 4 Pages 379-385
    Published: 1981
    Released: November 17, 2010
    JOURNALS FREE ACCESS
    TSUCHIYA, S., NAKAE, S., Koxno, T., TADA, K. and ONo, Y. Estimation of B-Cells Transformed by Epstein-Barr Virus in Patients with Congenital Agammaglobulinemia. Tohoku J. exp. Med., 1981, 135 (4), 379-385 - In vitro immunoglobulin synthesis was measured in lymphocytes from four patients with congenital agammaglobulinemia (cAy) stimulated by two different polyclonal B-cell activators, pokeweed mitogen (PWM) and Epstein-Barr virus (EBV). In PWM -stimulated cultures, patient T-cells treated with mitomycin C (MMC) were able to help the immunoglobulin (Ig) synthesis of normal B-cells. Patient B-cellenriched fraction not containing surface Ig positive cells did not produce Ig in combination with MMC-treated autologous or allogeneic T-cells. Patient lymphocytes were infected with EBV and the subsequent production of Ig was measured. In lymphocytes from control subjects, exponential growth of the cells having EBV-associated nuclear antigen (EBNA) was shown to be associated with an exponential increase in Ig secretion within 1 week after EBV infection. However, in lymphocytes from three of the four patients, it took 2, 4 and 10 weeks, respectively, until lymphocyte-transformation and subsequent Ig-secretion were observed. Lymphocytes from one patient were not transformed nor did they secrete Ig after EBV infection. These results may imply that a small number of B-cells are present in peripheral blood of most of patients with cAy, and that they are able to produce Ig after transformation by EBV which takes a much longer time than in controls. B cell; Epstein-Barr virus; congenital agammaglobulinemia
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  • WAKIO TORINUKI, TAKASHI MIURA
    1981 Volume 135 Issue 4 Pages 387-393
    Published: 1981
    Released: November 17, 2010
    JOURNALS FREE ACCESS
    TORINUKI, W. and MIURA, T. Singlet Oxygen and Ultraweak Chemiluminemence in Rat Skin. Tohoku J. exp. Med., 1981, 135 (4), 387-393-The intensity of ultraweak chemiluminescence (CL) was estimated in rat epidermal homogenate with a technique utilizing UV irradiation (in vitro and in vivo). CL markedly increased in the homogenate after irradiation. The CL intensity was enhanced to 300% by the substitution of deuterium oxide for H2O, and it was reduced to 50% when diazabicyclo-octane, a scavenger for singlet oxygen, was added. It was con-cluded that the weak CL would be attributable to singlet oxygen, and the deter- mination of the lipoperoxide in skin tissue by the method of the CL counting system was discussed. singlet oxygen; ultraweak chemiluminescence; rat skin
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  • TAKASHI SAITO, KIYOSHI OMURA, SEIJI HAYASAKA, HISAO NAKAJIMA, KATSUYOS ...
    1981 Volume 135 Issue 4 Pages 395-402
    Published: 1981
    Released: November 17, 2010
    JOURNALS FREE ACCESS
    SAITO, T., OMURA, K., HAYASAKA, S., NAKAJIMA, H., MIZUNO, K. and TADA, K. Hyperornithinemia with Gyrate Atrophy of the Choroid and Retina: A Disturbance in De Novo Formation of Proline. Tohoku J. exp. Med., 1981, 135 (4), 395-402-Two patients with hyperornithinemia and gyrate atrophy of the choroid and retina, the first report in Japan, were described. Serum ornithine was increased 10- to 15-fold and serum lysine was slightly decreased in the affected patients. Urinary excretion of ornithine was also markedly increased. There was no increase in serum proline after the oral loading of ornithine in the patients, whereas serum proline increased after the loading in normal controls. Ornithineketoacid transaminase (OKT) activity was markedly reduced in phytohemagglutinin transformed lymphocytes from the patients. In all of their parents and one of the siblings, OKT activity was found to be decreased to about half of the normal control values. These findings indicate that the pathway from ornithine to proline is a major route of ornithine catabolism in man. A possible pathogenesis of ocular disturbance in the patients with OKT deficiency was postulated.-hyperornithinemia; chorioretinal atrophy; ornithine-ketoacid transaminase; proline deficiency
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  • NOBUO KOJIMA, MAMORU ISEMURA, ZENSAKU YOSIZAWA, TERUO ONO, SHOJI SHINA ...
    1981 Volume 135 Issue 4 Pages 403-412
    Published: 1981
    Released: November 17, 2010
    JOURNALS FREE ACCESS
    KOJIMA, N., ISEMURA, M., YOSIZAWA, Z., ONO, T., SHINADA, S., SOGA, K., AOYAGI, Y. and ICHIDA, F. Distribution of Fibronectin in Fibrotic Human Livers at Various States. Tohoku J. exp. Med., 1981, 135 (4), 403-412-Fibronectin was isolated from human plasma by affinity chromatograpy with gelatin-coupled Sepharose 4B. Anti-fibronectin antiserum was prepared using rabbits, and human liver biopsy specimens at various stages of fibrosis were investigated for distribution of fibronectin by the indirect immunofluorescence technique. Fibronectin was absent in liver parenchymal cells, while it was detected intensely in reticulin and the area containing collagen fibers. The existence of fibronectin in the necrotic area and in prematured fibers suggests the earlier deposition of fibronectin than that of collagen at the locus of tissue repairing, and thus the important role of fibronectin at the early stage of fibrosis.-fibronectin; liver fibrosis; collagen: immunofluorescence
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  • KISHIRO NAGATA, YOSHIHIRO TAKARASHI, SHINOBU WAGA, MAKOTO FUJITA, TADA ...
    1981 Volume 135 Issue 4 Pages 413-417
    Published: 1981
    Released: November 17, 2010
    JOURNALS FREE ACCESS
    NAGATA, K., TAKAHASHI, Y., WAGA, S., FUJITA, M., KURONUMA, T. and AOYAMA, R. Monocyte Function in Idiopathic Nephrotic Syndrome in Childhood. Tohoku J. exp. Med., 1981, 135 (4), 413-417-The monocyte function in 2-15 year old patients with idiopathic nephrotic syndrome (INS) in childhood was studied in reference to the phagocytosis of sensitized red blood cells and NBT reduction. The monocyte function was found to be increased in fresh cases of INS. In the case of refractory INS not responding to steroid treatment, the monocyte function was normal or tended to be low.-monocyte; phagocytosis; NBT; reticuloendothelial system; idiopathic nephrotic syndrome
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  • TOSHIHIRO OKAMURA, HIROSHI TAJIMA, YOSHIYUKI IIJIMA, HARUTO GOMI, SHIR ...
    1981 Volume 135 Issue 4 Pages 419-422
    Published: 1981
    Released: November 17, 2010
    JOURNALS FREE ACCESS
    OKAMURA, T., TAJTIMA, H., IIJIMA, Y., GoMI, H., TOMINAGA, S., NIBHINARI, H. and OYAMADA, T. Standard Blood Pressure Curves of Schoolboys and Schoolgirlsin Akita Prefecture. Tohoku J. exp. Med., 1981, 135 (4), 419-422 Bloodpressure of 251, 742 schoolboys and schoolgirls aged 7 to 18 years was measuredin the year 1970. Analyzing these data, the authors obtained the percentile valuesclassified by age. The lowest hypertensive systolic blood pressures, which arerepresented by the 95th percentile, are 123, 126, 128, 128, 129, 134, 138, 144, 149, 152, 155, and 154 mmHg in boys from 7 to 18 years of age, respectively.The corresponding values are 123, 126, 128, 130, 134, 138, 139, 143, 144, 142, 142, and 141 mmHg in girls of the same ages. Girls' systolic blood pressureis higher than that of boys between the ages of 10 and 13 years. After the 14thyear of age, girls' systolic blood pressure does not increase any further, whereasboy's blood pressure continuously increases until 18 years. The present resultsdiffer in the following two points from the study in USA (1977): 1) The uniformrise of blood pressure with age is not observed. 2) Blood pressure, especiallysystolic pressure, is higher in girls than in boys between the ages of 10 and 13years, although it is higher in boys thereafter until 18 years.-bloodpressure in children; percentile values of blood pressure; juvenile hypertension
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  • KENICHI YAMADA, KAZUO MORI, YOSHIO GOTO
    1981 Volume 135 Issue 4 Pages 423-429
    Published: 1981
    Released: November 17, 2010
    JOURNALS FREE ACCESS
    YAMADA, K., MORI, K. and GOTO, Y. Platelet Aggregation and Plasma Levelsof Beta-Thromboglobulin in Diabetes Mellitus. Tohoku J. exp. Med., 1981, 135 (4), 423-429-In view of the tendency toward vascular complications in diabetesmellitus, we studied platelet aggregation and plasma beta-thromboglobulin (β-TG) levels in 35 healthy controls, 25 non-diabetic patients with ischemic heartdisease (IHD) and 85 diabetic patients. Blood platelets from diabetic patientsshowed no significant hyperaggregation induced by ADP, collagen or epinephrine, as compared with controls and non-diabetic patients with IHD, nor could bedemonstrated significant differences in platelet aggregation between diabetics withdiabetic microangiopathies and those without diabetic vascular complications.Significantly high levels of plasma β-TG were observed in diabetics in comparisonwith those in controls and non-diabetic patients with IHD. Patients withdiabetic microangiopathy had more significantly elevated β-TG levels thandiabetics without diabetic microangiopathies. Diabetics without diabeticmicroangiopathy had similar levels of β-TG to those of controls and non-diabeticpatients with IHD. High levels of plasma β-TG in diabetics with diabetic microangiopathyseem to indicate a platelet hyperfunction in vivo due to diabeticvascular complications, -platelet aggregation; diabetic microangiopathy; beta-thromboglobulin; diabetes mellitus; ischemic heart disease
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  • MASAI KOSEKI, SHIGERU INO, ATSUSHI KIMURA, KOICHI TSURUMI
    1981 Volume 135 Issue 4 Pages 431-439
    Published: 1981
    Released: November 17, 2010
    JOURNALS FREE ACCESS
    KOSEKI, M., INO, S., KIMURA, A. and TSURUMI, K. Abnormal Urinary Excretionof Sialoglycoconjugates in Patients with Mucopolysaccharidosis. Tohoku J.exp. Med., 1981, 135 (4), 431-439-Abnormal urinary excretion of sialoglycoconjugateswas observed in four patients with mucopolysaccharidosis. Urinarysialoglycoconjugates were fractionated into 8 fractions by sequential gel filtrationon Sephadex G-25, G-50 and by Dowex 1 ion-exchange chromatography.The comparison of the amounts of these fractions indicated that the fraction richin mannose and glucosamine contents contributed to the increased urinary excretionof sialoglycoconjugates in patients with mucopolysaccharidosis. The majorcomponent of this fraction was disialyl-oroso-N-octaose which was a representativeoligosaccharide side chain of glycoproteins with an Asn-GlcNAc linkage. Undoubtedlythis abnormality is the secondary lesion of mucopolysaccharidosis, butit is conceivable that the disturbed metabolism of sialoglycoprotein is closely relatedto the pathogenesis of these diseases.-lysosomal storage diseases;mucopolysaccharidosis; sialyloligosaccharides; sialoglycoconjugates
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  • HIROKI WATANABE, TERUO MISHINA, HIROSHI OHE, HIROTAKA ARAKI, MASAHIRO ...
    1981 Volume 135 Issue 4 Pages 441-442
    Published: 1981
    Released: November 17, 2010
    JOURNALS FREE ACCESS
    WATANABE, H., MISHINA, T., OHE, H., ARAKI, H. and NAKAO, M. A Cluster-ing of Prostatic Cancer in an Area with Many Manganese Mines. Tohoku J. exp. Med., 1981, 135 (4), 441-442 In the process of our mass screening trials for prostatic diseases, a significant clustering of prostatic cancer in a town in the suburbs of Kyoto was detected. Upon survey a possible association was found between the locations where the patients lived and the manganese ore deposits distributed in the town.-prostatic cancer; clustering; manganese
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  • SHOZO NAKAMURA
    1981 Volume 135 Issue 4 Pages 443-444
    Published: 1981
    Released: November 17, 2010
    JOURNALS FREE ACCESS
    NAKAMURA, S. Influence of Exercise and Smoking on High Density Lipoprotein Cholesterol in University Students. Tohoku J. exp. Med., 1981, 135 (4), 443-444 - High density lipoprotein (HDL) cholesterol was measured in male university students with normal body weight and daily alcoholic intake of less than 22g. HDL cholesterol measured by the precipitation method was significantly higher in non-smokers with active exercise and in smokers with active exercise than in smokers with inactive exercise. Values more than 60mg/100ml were observed significantly more frequently in non-smokers with active exercise, in non-smokers with inactive exercise, and in smokers with active exercise than in smokers with inactive exercise. Thus association of HDL cholesterol with exercise and smoking is already present in university students.-high density lipoprotein cholesterol; exercise; smoking; university student
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  • KENJI MIZUNO, MITSUHIRO GOTOH, SOITSU FUKUCHI
    1981 Volume 135 Issue 4 Pages 445-446
    Published: 1981
    Released: November 17, 2010
    JOURNALS FREE ACCESS
    MIZUNO, K., GOTOH, M. and FUKUCHI, S. Brain Angiotensin-Converting Enzyme Activity in Experimental Hypertensive Rats. Tohoku J. exp. Med., 1981, 135 (4), 445-446 Angiotensin-converting enzyme (ACE) activity was measured in six areas of the brain of normotensive and experimental hypertensive rats; one-clip, one-kidney (1-c, 1-k) and one-clip, two-kidney (1-c, 2-k) Goldblatt hypertensive (GH) rats. ACE activity was consistently high in the thalamus of normotensive and both 1-c, 1-k and 1-c, 2-k GH rats. However, the enzyme activity in the hypothalamus of 1-c, 2-k GH rats was significantly higher than that of normotensive rats, while there was no significant difference in the enzyme activity between normotensive and 1-c, 1-k GH rats. These results demonstrate that in 1-c, 2-k GH rats, increased ACE activity in the brain may play a central role in the hypertension.-angiotensin-converting enzyme; brain; hypertensive rats
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