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Volume 190 (2000)
- Issue 4 Pages 239-
- Issue 3 Pages 169-
- Issue 2 Pages 65-
- Issue 1 Pages 1-
Volume 190, Issue 3
March
Displaying 1-8 of 8 articles from this issue
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Regular Contributions
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Koichi Chida, Masahiro Sai, Haruo Saito, Kei Takase, Masayuki Zuguchi, ...2000 Volume 190 Issue 3 Pages 169-176
Published: 2000
Released on J-STAGE: May 24, 2005
JOURNAL FREE ACCESSQuantitative digital subtraction angiography (DSA) image analysis based on densitometry is widely accepted and used. For the densitometoric DSA image analysis, it is required that there is a linear relationships between the pixel values on DSA images (DSA values) and contrast medium iodine concentration or the thickness of the vessels or the chambers filled with contrast material. We studied on relationship between the DSA value and iodine concentration especially in high iodine concentration. As for the relationship between DSA values and iodine concentration on the DSA images at low concentration, DSA phantom had a good linear relationship. However, the relationship at the high iodine concentration, DSA phantom sometimes lost this linear relationship. Our results suggested that it was necessary to identify relationship between DSA values and iodine concentration in each DSA system, especially in high iodine concentration setting, for densitometoric DSA image analysis.View full abstractDownload PDF (411K) -
Hitoshi Kubota, Kyoji Okada, Kozo Sato, Masato Sageshima2000 Volume 190 Issue 3 Pages 177-184
Published: 2000
Released on J-STAGE: May 24, 2005
JOURNAL FREE ACCESSTo investigate whether the expression of human leukocyte antigen-DR (HLA-DR) in synovial tissues obtained at synovectomy contributes to the clinical features of patients with rheumatoid arthritis (RA), immunohistochemical analysis was performed to determine the intensity and pattern of HLA-DR expression in synovial tissue from 96 patients with RA. The clinical features before and 1 year after the synovectomy were investigated. At the time of the surgery, duration of morning stiffness was associated with the degree of HLA-DR expression in synovial lining layer, and this synovial lining expression of HLA-DR was more frequently observed in elbow and wrist joints than in knee joint. In patients who underwent knee synovectomy, erythrocyte sedimentation rate and C-reactive protein level one year after the surgery were significantly higher in the patients with intense expression of HLA-DR in the synovial lining. Our findings showed that the expression of HLA-DR in the synovial lining contributes to several clinical features, and the expression in large joint such as knee may related with disease course of patients with RA.View full abstractDownload PDF (1060K) -
Fumihiko Mizutani, Kohachiro Sugiyama, Kenji Goto, Toshihiro Ando, Koj ...2000 Volume 190 Issue 3 Pages 185-192
Published: 2000
Released on J-STAGE: May 24, 2005
JOURNAL FREE ACCESSThe prevalence of serum GB virus C (GBV-C)/Hepatitis G virus (HGV) RNA and anti-E2 was investigated in Japanese children younger than 16 years of age without a history of blood transfusion and the family members of serum GBV-C/HGV RNA-positive children. The prevalences of serum GBV-C/HGV RNA and anti-E2 were 0.5% (5/1000) and 0% (0/330), respectively. Viral RNA was also detected in the mothers of all five GBV-C/HGV RNA-positive children and in two of their siblings. Sequence determinations indicated the likelihood of mother-to-infant transmission in all cases. The presence of the virus persisted for at least 10-18 months in all 5 children, without any appearance of anti-E2.View full abstractDownload PDF (510K) -
Ryo Konno, Shinji Sato, Akira Yajima2000 Volume 190 Issue 3 Pages 193-203
Published: 2000
Released on J-STAGE: May 24, 2005
JOURNAL FREE ACCESSThe current standards for surgical procedures and lymph node dissection of endometrial cancer in Japan were investigated using a questionnaire survey. The estimated clinical stages used in the questionnaire were predicted from preoperative diagnostic imaging, histopathology of endometrial biopsies and intraoperative findings using a new classification, Federation Internationale de Gynecologie et d'Obstetrique (FIGO) in 1988. Questionnaires were mailed to 235 institutions, and 212 institutions (90.2%) responded. As a standard surgery for endometrial cancer, institutions performed simple total hysterectomy or semi-radical hysterectomy and bilateral adnexectomy, which accounted for 86% of all respondents. For stage I carcinoma, simple (44%) or semi-radical (47%) hysterectomy was carried out in 91% of institutions, while radical hysterectomy was selected in 84% of institutions when stage II carcinoma was diagnosed clinically. The consensus of this survey was that dissection of both the para-aortic and pelvic lymph nodes can be omitted in G1 cases showing lesions confined to the endometrium, and that pelvic lymph nodes should be dissected, but para-aortic lymph node dissection could be omitted in G1 or G2 cases demonstrating myometrial invasion of 1/2 or less. Moreover, findings from this survey suggest that biopsy or dissection of the para-aortic lymph nodes was required in G3 cases, or in those patients diagnosed with myometrial invasion more than 1/2.View full abstractDownload PDF (611K) -
Sumiko Yoshida, Yohtaro Numachi, Hiroo Matsuoka, Mitsumoto Sato2000 Volume 190 Issue 3 Pages 205-212
Published: 2000
Released on J-STAGE: May 24, 2005
JOURNAL FREE ACCESSCliff avoidance reaction (CAR), an index of behavioral teratology in rodents, can be impaired by motor, arousal, or cognitive dysfunction. We formerly reported subchronic administration of methamphetamine (MAP) induced the CAR impairment, which might reflect MAP-induced cognitive dysfunction, in three strains of rats. In this study, the effects of subchronic MAP treatment on the behavioral sensitization in stereotypy (stereotypy sensitization) and CAR were examined in two inbred strains of male mice; C57BL/6J(C57) and DBA/2J(DBA). The animals received 4 mg/kg/day MAP intraperitoneally for 28 days. There were apparent strain differences in the development of stereotypy sensitization induced by chronic MAP; DBA mice developed stereotypy sensitization quickly, but C57 did not. Unlike rats, neither strains of mice showed the CAR impairment. These results suggest that chronic MAP (4 mg/kg) administration did not introduce any cognitive dysfunction measured by CAR in the two inbred mice, DBA and C57. The discrepancy between rats and mice is still unclear. It might relate to the species-selective effect of MAP on the CAR impairment. Further studies should to be required.View full abstractDownload PDF (459K) -
Inci Senyüz Simsek, Sevda Menevse, Feride Iffet Sahin2000 Volume 190 Issue 3 Pages 213-222
Published: 2000
Released on J-STAGE: May 24, 2005
JOURNAL FREE ACCESSHelicobacter pylori (H. pylori) infection is the most common gastrointestinal tract infection which plays an important role in the ethiopathogenesis of peptic ulcer and gastritis. In recent years, molecular biological methods have been presented for detection of H. pylori in addition to histopathological and microbiological methods. Among these methods, polymerase chain reaction (PCR) and following restriction fragment length polymorphism analyses (RFLP) are highly sensitive methods for diagnosis and follow up of patients. In this present study our aim was to amplify H. pylori urease A and B genes by PCR and perform RFLP analysis. Gastric biopsy specimens from 17 female and 18 male patients were included in the study. Amplified PCR products were subjected to RFLP analysis and typing of the bacteria in pre and posttreatment specimens were performed. H. pylori urease A and B gene amplification was observed in 32 pretreatment samples and in 8 of 21 posttreatment specimens. As a result, PCR is a sensitive method to determine the H. pylori infection. RFLP, which is another effective method in order to demonstrate the reinfection of H. pylori.View full abstractDownload PDF (1469K) -
Hiroshi Tomita, Toshiaki Abe, Makoto Tamai2000 Volume 190 Issue 3 Pages 223-229
Published: 2000
Released on J-STAGE: May 24, 2005
JOURNAL FREE ACCESSWe investigated whether retinal pigment epithelial (RPE) responds to ceramide, a known second messenger of apoptosis. RPE cells were isolated by 6-8 day old Long Evans rat eye. We used MTS assay for viability test, and used Hoechst 33552 and propidium iodide for apoptic cell staining. In cultured rat RPE cells, the addition of membrane-permeable ceramide induced apoptosis-like cell death rapidly. RPE cell death was dependent on C2-ceramide concentration. The effective dose (ED50) of C2-ceramide was 23.64 μM. Ceramide-induced RPE cell death was inhibited by zVAD-fmk, a CPP32-like protease inhibitor. Our findings indicated that ceramide in RPE cell death functions upstream of CPP32-like proteases.View full abstractDownload PDF (481K) -
Chika Kinugawa, Takashi Murakami, Kunihiro Okamura, Akira Yajima2000 Volume 190 Issue 3 Pages 231-238
Published: 2000
Released on J-STAGE: May 24, 2005
JOURNAL FREE ACCESSThe average age for the onset of menopause in humans is about 51 years. On the other hand, premature ovarian failure (POF) is generally defined as the onset of menopause before the age of 40 years. Telomeres have been extensively examined as a mitotic clock. Telomeric DNA is elongated by telomerase. We analyzed the telomerase activity of 20 patients with normal ovaries and 5 patients with POF. Telomerase activity was present in the normal ovaries, however it decreased with age. Eight normal ovaries under 38 years of age showed significantly higher telomerase activity among the women with a regular menstrual cycle. Two POF patients showed high telomerase activity and 3 showed low telomerase activity. Our findings indicate that telomerase is present in the normal human ovary and that telomerase activity decreases with age. Patients with follicle dysfunction showed high telomerase activity and those with follicle depletion showed very low telomerase activity. Based on these results, we speculated that the decline of telomerase activity in the ovary is related to primordial follicle depletion with age and telomerase activity can be used as a marker of the ovarian functional age.View full abstractDownload PDF (884K)
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