The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
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Volume 110, Issue 3
Displaying 1-12 of 12 articles from this issue
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  • Effect of Heparinoid on Free Thyroxine Concentration in Human Serum
    TOSHIRO SAKURADA, SUMIO TAYAMA, SHINTARO SAITO, TATSUO TORIKAI
    1973 Volume 110 Issue 3 Pages 209-224
    Published: 1973
    Released on J-STAGE: November 28, 2008
    DOIhttps://doi.org/10.1620/tjem.110.209
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    Sodium salt of dextran sulfate ester (MDS-Kowa, Kowa Co., Japan) was administered intravenously to 4 euthyroid subjects, 7 patients with hyperthyroidism and 5 patients with hypothyroidism. Serum FFA rose to the level 4-6 times higher than the preinjection level 15min after the intravenous injection. At the same time, elutable fraction (EF) of T4, free T4, and binding capacity and concentration of TBPA increased, while binding capaty of TBG decreased. Addition of dextran sulfate to serum in vitro caused no change in binding capacity of TBG and EF of T4. Addition of palmitic acid to serum in vitro with increasing concentration resulted in a gradual decrease in the distribution of 131I-T4 to TBG and binding capacity of TBG, while EF of T4 gradually increased. Intravenous injection of dextran sulfate ester following the prior injection of protamine sulfate in dog caused a mild rise in serum FFA, but failed to increase the EF of T4. When reverse-flow paper electrophoresis was carried out after addition of 14C-palmitic acid to sera of normal and TBG-deficient subjects, it was confirmed that palmitic acid bound to albumin and TBG fraction. These results suggest that dextran sulfate ester raises serum FFA first, then the increased FFA combines with TBP at least with TBG, thus competing for binding sites with T4, and released T4 in turn increases free T4 level in the serum.
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  • The in vitro Deiodination of Thyroxine and Triiodothyronine
    TOSHIRO SAKURADA, YASUO SUZUKI, SHINTARO SAITO, TATSUO TORIKAT
    1973 Volume 110 Issue 3 Pages 225-234
    Published: 1973
    Released on J-STAGE: November 28, 2008
    DOIhttps://doi.org/10.1620/tjem.110.225
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    Deiodination of 131I-T3 and 131I-T4 in the liver, kidney and spleen homogenates obtained from normal and thyroidectomized rats was studied before and after the administration of l-T3 and l-T4. Deiodination of 131I-T3 in the liver and spleen homogenates was decelerated by the administration of l-T3 or l-T4 both in normal and thyroidectomized rats. Deiodination of 131I-T3 and 131I-T4 in kidney homogenates was accelerated by the administration of l-T3 or l-T4 both in normal and thyroidectomized rats. These results suggest that difference of metabolism between T3 and T4 would exist in thyroid disorders.
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  • The Effect of Autonomic Drugs on the Canine Electroureterogram
    SEIGI TSUCHIDA, HIROATSU SUGAWARA, SATOSHI MATSUMURA, YOSHITAKA SHIBUY ...
    1973 Volume 110 Issue 3 Pages 235-245
    Published: 1973
    Released on J-STAGE: November 28, 2008
    DOIhttps://doi.org/10.1620/tjem.110.235
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    Excluding the influence of changing urine flow by ligating the renal vein, canine electroureterographic studies were conducted to evaluate the effects of autonomic drugs upon the ureteral function. Intra-aortic administration of epinephrine, norepinephrine, phenylephrine, some amounts of acetylcholine, and dimethylphenylpiperazinium (DMPP) accelerated ureteral peristalsis. In the preadministration of phentolamine, epinephrine prolonged discharge intervals, and the response was suggestive of β effect. Phentolamine, propranolol, neostigmine, atropine, hexamethonium and tetrodotoxin caused no changes in electroureterograms.
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  • Human Mast Cells in the Allergic Nasal Mucosa
    TOMONORI TAKASAKA, YONOSUKE UENO
    1973 Volume 110 Issue 3 Pages 247-261
    Published: 1973
    Released on J-STAGE: November 28, 2008
    DOIhttps://doi.org/10.1620/tjem.110.247
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    The human mast cells in the allergic nasal mucosa contained many granules with various features. On the basis of electron microscopic observations, we have classified these granules into two groups: “normal” and “altered”. The former group consisted of granules which showed comparable structure to those in the normal environment. They contained electron dense, homogeneous material or less dense amorphous material. Many of them were surrounded only by a unit membrane, but sometimes a very thin envelope of the matrix was discernible immediately inside the unit membrane. The latter group comprised granules with marked ultrastructural changes. The swelling of the granules showing a clear halo formation around the matrix was a common feature. The internal substance obviously lost its electron density and became fibrillar or granular in appearance. These “altered” granules were fairly predominant in number as compared with those with “normal” structure. The ultrastructural changes found in the present study was quite smilar to those observed in the experimental degranulation studies caused by compound 48/80. Therefore, it seems reasonable to expect that mast cell granules of the allergic nasal mucosa are also released from the cytoplasm, responding to the antigenantibody reaction.
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  • Studies on the Phospholipase A2 in Pyloric Caeca of the Starfish Asterina pectinifera
    HIROAKI OKABE, AKIO NOMA
    1973 Volume 110 Issue 3 Pages 263-271
    Published: 1973
    Released on J-STAGE: November 28, 2008
    DOIhttps://doi.org/10.1620/tjem.110.263
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    The occurrence of remarkable activity of phospholipase A2 was found in fresh homogenate of pyloric caeca of starfish, mainly using lecithin labeled with 3H-palmitic acid at α-position as substrate. The effects of incubation time, enzyme (protein) concentration, divalent cations, and EDTA on the hydrolysis of substrate were investigated. The activity was markedly inhibited with EDTA and was dependent on divalent cations such as Ca++. Sodium deoxycholate and certain organic solvents such as benzene, diethylether etc. also activated the enzyme. The optimal pH was about 8. The occurrence of low activity of lyso-phospholipase was also found in fresh homogenate but little lipase activity was detected.
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  • Choline Phosphokinase, Phosphorylcholine Cytidyltransferase and CDP-choline: 1, 2-diglyceride Cholinephosphotransferase Activity in Developing Rat Lung
    NOBUKAZU CHIDA, HARUKO HIRONO, YASUHIRO NISHIMURA, TSUNEO ARAKAWA
    1973 Volume 110 Issue 3 Pages 273-282
    Published: 1973
    Released on J-STAGE: November 28, 2008
    DOIhttps://doi.org/10.1620/tjem.110.273
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    CHIDA, N., HIRONO, H., NISHIMURA, Y. and ARAKAWA, Ts., Choline Phosphokinase, Phosphorylcholine Cytidyltransferase and CDP-choline-1, 2-diglyceride Cholinephosphotransferase Activity in Developing Rat Lung. Tohoku J. exp. Med., 1973, 110 (3), 273-282-Incorporation of choline-methyl-14C into phosphorylcholine, CDP-choline, and phosphatidylcholine by lung tissue of developing rats was studied. Choline phosphokinase, phosphorylcholine cytidyltransferase, and CDP-choline: 1, 2-diglyceride choline phosphotransferase were determined in the lung tissue of developing rats. Results indicated that phosphorylcholine cytidyltransferase was the rate-limiting enzyme in the reactions-choline→phosphoryl-choline→CDP-choline→phosphatidylcholine
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  • A Metanephrine Test for a Screening of Catecholamine-producing Tumors
    TATSUO SATO, KAORU YOSHINAGA
    1973 Volume 110 Issue 3 Pages 283-287
    Published: 1973
    Released on J-STAGE: November 28, 2008
    DOIhttps://doi.org/10.1620/tjem.110.283
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    A simple method is described for the determination of free total metanephrine (metanephrine plus normetanephrine) in urine. The method includes direct oxidation of 3-methoxyamine metabolites of catecholamine to vanillin in alkaline urine and extraction of formed vanillin with toluene which is followed by spectrophotometrical assay. The method has been proved its reliability and simplicity for screening of patients with catecholamine-producing tumors.
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  • Clinical Evaluation of 99mTc-labeled Sodium Tripolyphosphate as a Bone Imaging Agent
    MAMORU NAKAMURA, YOSHIKAZU SAWAI, MITSUNOBU ABE, HIROSRI TAKAHASHI, NO ...
    1973 Volume 110 Issue 3 Pages 289-296
    Published: 1973
    Released on J-STAGE: November 28, 2008
    DOIhttps://doi.org/10.1620/tjem.110.289
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    99mTc complex of tripolyphosphate and stannous chloride has been evaluated as a bone seeking agent. This compound is prepared simply using “kit” method. Animal studies show that bone/muscle, bone/blood and bone/liver ratio are high enough to delineate the skeletal systems. This compound is promptly excreted in the urine. Various bone diseases are delineated by rectilinear scanning. A blood pool background is high at the end of two hours after injection. The optimal time for scanning is about three to six hours after injection. The image with 99mTc-STPP is comparable to that with 87mSr.
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  • Serial Surveys for Hepatitis B Antigen in an Institution for the Mentally Retarded: A Detection of the Antigen in Saliva
    TASUKE KONNO, RHOICHI SHIRACHI, NAKAO ISHIDA
    1973 Volume 110 Issue 3 Pages 297-302
    Published: 1973
    Released on J-STAGE: November 28, 2008
    DOIhttps://doi.org/10.1620/tjem.110.297
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    Serial tests for the HB antigen were carried out in an institution for the mentally retarded. During the study the frequency of the antigen carrier was found to be 23%, of which one third had a persistent antigenemia. The antigenemia was found more frequently in the younger (under 10 years of age) than in the older. In contrast, the incidence of the antibody carrier was higher in the older. The antigen was acquired in 2 newly admitted patients 3 and 4 months after institutionalization. The HB antigen was detected by means of RIA in saliva obtained from 5 patients who were positive for the antigen in serum. The salivary antigen may be an important source for spreading the antigen in a closed environment.
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  • Adrenaline and Noradrenaline Secretion of the Adrenal Gland in Conscious Dogs in Response to Pilocarpine and Nicotine
    SHINJI NARITA, OKIHIKO SHIBATA, SHINJI WAKI, KINVA EGASHIRA, TATUZI SU ...
    1973 Volume 110 Issue 3 Pages 303-305
    Published: 1973
    Released on J-STAGE: November 28, 2008
    DOIhttps://doi.org/10.1620/tjem.110.303
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    Conscious dogs were injected intravenously with 1.0mg/kg of pilocarpine or 0.3mg/kg of nicotine. Adrenal venous effluent was collected before and after the injection and analyzed for adrenaline and noradrenaline fluorimetrically. After the injection of pilocarpine a marked increase in adrenaline secretion with an inconsistent and slight increase in noradrenaline secretion was observed. Following the injection of nicotine the adrenaline and noradrenaline secretion increased markedly, the increase in the former being predominant.
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  • Effect of Glucose Infusion on Adrenaline and Noradrenaline Secretion from the Adrenal Gland in Conscious Dogs
    TAEKO HIROSE, KAZUNORI TAMURA, HIROYUKI IKEDA, TATUZI SUZUKI
    1973 Volume 110 Issue 3 Pages 307-308
    Published: 1973
    Released on J-STAGE: November 28, 2008
    DOIhttps://doi.org/10.1620/tjem.110.307
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    Conscious dogs were infused intravenously with glucose solution over 10 minutes. The samples of the adrenal venous blood were collected before and after the infusion of glucose and analyzed differentially for adrenaline and noradrenaline by the use of fluorimetric method. The blood glucose level was elevated by glucose infusion from 69-92mg/100 ml to 270-338mg/100 ml and then subsided gradually to the pre-infusion level. However, no definite changes in adrenaline and noradrenaline secretion were observed after the infusion of glucose.
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  • Pattern of β-Galactosidase Deficiency in GM1-Gangliosidosis Type 1 and Type 2
    TADAO ORII, KAZUKO SUKEGAWA, RYOJI MINAMI, SATOSHI TSUGAWA, TOORU NAKA ...
    1973 Volume 110 Issue 3 Pages 309-310
    Published: 1973
    Released on J-STAGE: November 28, 2008
    DOIhttps://doi.org/10.1620/tjem.110.309
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    Despite distinctly different clinical phenotypes, enzymatic variations in GM1-gangliosidosis type 1, type 2 and new type of mucolipidosis could not be detected in pH activity curves or in starch-gel electrophoresis.
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