1. Hydrazinolysis of monosaccharides resulted in the degradation of 47.5 per cent of N-acetylglucosamine, 32.7 per cent of N-acetylgalactosamine, 20.9 per cent of galactose, 12.9 per cent of rhamnose and 43.5 per cent of glucuronic acid, respectively. 2. Small amounts of 2, 5-anhydrosugars, besides other unknown compounds, were found in all the reaction products of the hydrazinolysis of the monosaccharides and small amounts of pentoses and hexoses were also detected in some of the reaction products. 3. All the hydrazinolyzed products of the monosaccharides, more or less, contained the nitrogen, which was unable to determine by the Van Slyke procedure. 4. Almost all of the hexosamines and some of the other sugars in the hydrazinolyzates of the monosaccharides were absorved on Amberlite IIt 120 (H+) resins and were easily recovered by the elution with hydrochloric acid or sulfuric acid. 5. Per cent contents of the hexosamines, which could be determined as 2, 5-anhydrohexoses by the direct deamination with nitrous acid, in total hexosamines in the hydrazinolyzates of N-acetylglucosamine and N-acetylgalactosamine amounted to 80.0 per cent and 71.4 per cent, respectively.
We examined Bial's reaction applied for estimation. of sialic acid in the cerebrospinal fluid samples, consisting in whole and protein-free cerebrospinal fluid and the protein in it. We concluded it was difficult to estimate sialic acid in the samples of whole and protein-free cerebrospinal fluids by this reaction. We suppose the free or non-protein-bound sialic acid is inconstant and a large part of the sialic acid in the cerebrospinal fluid is in the protein-bound form.
We examined the diphenylamine reaction for determination of sialic acid in various samples of the human cerebrospinal fluid. It is recognized that the reaction is an unsatisfactory method for this purpose, except in estimating the acid content in cerebrospinal fluid protein or Niazi-State's fraction. In the cases trichloracetic acid is present in the samples or the reaction system, the blue or indigo-blue coloration tends to intensify, even when the main coloration is purple, and the extinction value is heightened in its presence, not only at 530mμ, but also at 660 mμ. It is necessary to investigate this tendency furthermore. Though there is a possibility that some free sialic acid exists in the cerebrospinal fluid, it might be denied that free sialic acid exists in a quantity several times larger than protein-bound sialic acid.
Correlation coefficients were calculated between national average supplies of 10 foodstuff groups and corrected death rate for 30-69 age group from cerebrovascular lesions and arteriosclerotic heart disease, among 20 Western countries. Results follow: 1. No significant correlation of the corrected death rates is found between cerebrovascular lesions and arteriosclerotic heart disease. 2. There are high correlation between male and female in both corrected death rates for cerebrovascular lesions and for arteriosclerotic heart disease. 3. There are some correlations among foodstuff groups by nations, for instance, sugar and syrups-meat-milk (positive correlation), and cereals-meat (negative correlation). 4. Nations which consume more cereals and less fats and oils tend to have a high mortality from cerebrovascular lesions. 5. Nations which consume less potatoes and other starchy roots, more sugar and syrups and probably more meat, eggs and milk tend to have a high mortality for arteriosclerotic heart disease. These results are discussed on the data of peculiar condition in Japan.
The problem whether circulating angiotensin is, as catecholamines, excreted in urine was investigated in the hope that the study of renin-angiotensin system would be greatly facilitated, if it is the case in human or in experimental animals. Though the assay method used here could accurately estimate angiotensin in urine in low concentrations down to 0.05 μg./100 ml., no angiotensin was found in the urine of healthy or hypertensive subjects including a patient with unilateral renal arterial stenosis with severe hypertension. Infusion of angiotensin in doses enough to cause violent elevation of blood pressure resulted in no definite excretion of it in urine. It is concluded that the circulating angiotensin is not excreted in urine in any significant amount.
Purification of Sendai virus by using aluminum phosphate gel was studied to remove the host components which are derived from chick embryos and are hardly removable by fractional centrifugation. As a result, 10 mg of aluminum phosphate gel prepared under the standard condition has been shown to adsorb almost 15, 000 hemagglutinin units of the virus at maximum. The adsorption and elution occurred almost immediately at 4°C whenever the pH and salt concentrations of the medium are optimal. When the virus adsorbed to a large amount of gel was eluted fractionally with 0.25 M phosphate buffer of pH 8.0, highly purified virus, in the sense of hemagglutinin units per mg nitrogen, was constantly obtained. This value reached 682, 000. The final fraction revealed a single component of 1, 100 S in the sedimentation pattern. Specificity of viral protein in the behavior at the time of adsorption and elution was discussed.
The fluorescent spot technique was developed so that the flocculation reaction between viral particles and antibodies could be seen quickly and simply on a glass slide. This technique was readily available for testing the purity of the Sendai virus during its purification. Immune serum prepared against normal sedimentable material of uninfected eggs was particularly useful to test for the presence of host components in purified viral suspensions. A highly purified fraction of high HA titer obtained by adsorption on, and elution from aluminum phosphate gel, was shown by this spot technique to be almost free from normal components. When such a purified preparation was used for HAI titration, the antibody titer was low because of the increased viral antigenic surface around the particles. The hemolytic properties known to be associated with Sendai virus were also reduced in such a purified preparation.
1) Polarographical activity in catalytic wave of ‘mucoprotein complex’ of rat skin of different ages was compared; the second wave at the potential of -1.55 - -1.60 V decreased with increasing age. A possibility of this qualitative change in non-collagenous protein moiety was discussed. 2) The mucoprotein complex of normal rabbit skin and that of the granulation tissue developed after injury of the skin were compared in polarographical activity, analytical values of carbohydrate component and the electrophoretic pattern. The polarographical activity increased with an increase of a glycoprotein resembling the α-glycoprotein of serum in the granulation tissue after the tenth to fifteenth day of injury and decreased with the process of wound healing. These results were discussed.