The ADP and ATP levels of erythrocytes were determined by the column-chromatography in patients with uremia associated with chronic renal failure, in order to study the mechanism of the increased hemolysis in uremia. The in vitro incorporation of radiophosphorus in ADP and ATP of erythrocytes was also studied. The ADP and ATP levels of erythrocytes were slightly elevated in patients with uremia, averaging 11.9mg/100ml erythrocyte and 98.1 mg/100 ml erythrocytes, respectively. The ATP/ADP ratio was within normal limits. When normal erythrocytes were incubated with 32P in vitro for 15minutes at 37°C, no radioactivity was demonstrated in the 5/1, 000 N HCl fraction containing inorganic phosphorus and AMP. However, when erythrocytes of patients with uremia were incubated with 32P for 15 minutes, a large amount of radioactivity was still present in the 5/1, 000 HCl fraction. The in vitro incorporation of radiophos-phorous in ADP and ATP of erythrocytes was markedly reduced in patients with uremia. These results demonstrate the decrease of the phosphorylating activity of erythrocytes in patients with uremia. When normal erythrocytes were suspended in uremic plasma and incubated with radiophosphorus, the in vitro incorporation of 32P was markedly reduced, suggesting the inhibitory effect of the increased non-protein nitrogen or toxic products in uremic plasma on the phosphorylating activity of erythrocytes. It seems likely that this decrease of the phosphorylating activity of erythrocytes might play some roles in the mechanism of the increased hemolysis in uremic patients.
This paper reports the electrophysiologic activity of the canine stomach after transaction at various levels followed by end-to-end anastomosis. The spike discharges remain little changed when the transection line is placed in the upper third of the stomach or adjacent to the pyloric ring. When the stomach is divided and anastomosed in the corpus or the pyloric antrum 3-4cm apart from the pyloric ring, on the other hand, the activity is considerably modified in the portion distal to the line of anastomosis. The changes on this occasion include an increase of the discharge interval and an appearance of antiperistaltic discharges, probably representing automatism of the stomach. Although such changes are subject to physiological adaptation, they persist for a considerably long period after the operation.
In order to ascertain a correlation of the juxtaglomerular apparatus, renin or angiotensin and aldosterone to the arterial pressure, a series of ten groups of experiment was performed by rabbits and rats. Hypertrophy of the zona glomerulosa of the adrenal cortex and an elevated plasma aldosterone level were observed in rabbits receiving angiotensin IT. In the rats with Goldblatt hypertension, a slight increase in the juxtaglomerular granulation index in the clamped kidney and a marked decrease in the untouched contralateral kidney were observed. No appreciable change was detected in the zona glomerulosa. It was suggested that renin and/or angiotensin are potent stimulants of aldosterone secretion, but there was no evidence that the renin-angiotensin-aldosterone system is essential to induce or maintain renal hypertension. And an increase in the potassium content of the aortic wall in the rats with experimental hyperten-sion of Goldblatt and DOCA plus NaCl treatment seems to have a close relation to the development of hypertension.
This report concerns with a one year and 3 months old female infant. She was thought to have seizure attacks and right hemiparesis due to kinking of the internal carotid artery which was found by carotid angiography. The left laternal angiogram showed a marked tortuous course of the internal carotid artery imediately above the carotid bifurcation. Pneumoencephalogram suggested severe degree of atrophy of the left cerebral hemisphere. An operation was performed to correct the buckling of this vessel by traction and fixation to straighten its course. Postoperative course was un-eventful. Previous convulsions disappeared with improvement of the right hemiparesis.
One cell culture line was obtained from ascites fluid of a case diagnosed as reticulosarcomatosis. After about 50 days lag period, we were able to obtain a cell line which could grow continuously in vitro. This cell line has grown only in a floating state. At the present time, the doubling time of the culture is within 30 hours and the cells can multiply actively. Chromosomal analysis showed that the modal number of this line was 46, but the karyotype was not the same as that of the normal human cells. So far this pseudo-diploidal character has not changed in its successive culture.
The β-glucuronidase activity found in bile of patients with calcium bilirubi-nate stones is presumed to be significantly related to the pathogenesis of this type of gallstones. In this connection, this study presents evidence to show that β-glucuronidase in bile of those patients is predominantly of bacterial origin, mostly related to E. coli in bile, in contrast to the ease of cholesterol stones in which the β-glucuronidase activity of bile is usually that of tissue β-glucuronidase. These results were obtained by comparative studies, on an individual basis, of the β-glucuronidase activities of bile, E. coli isolated from bile, serum and liver tissue.
The excretion of doma in 24 hour urine was determined in 15 normal subjects, 32 patients with hypertension including 5 cases of pheochromocytoma, and 10 patients with hyperthyroidism, using the high-voltage paper electrophoretic method. Estimation by this method revealed that the excretion of doma in the normal subjects amounted 40_??_180μg/day (mean 99.9±43.1μg). In hyperthyroid or hypertensive patients except the cases of pheochromocytoma, the excre-tion of doma was almost equal to that in normal subjects. But the excretion of doma, as well as catecholamines and the other metabolites of catecholamines, was markedly increased in patients with pheochromocytoma. Moreover, in this disease the urinary excretion ratio of catecholamines/doma/methoxycatechol-amines/VMA was always in good agreement with the reported excretion ratio obtained in man after the infusion of catecholamines. From these findings, it was supposed that there were no overproduction and no metabolic disturbances of catecholamines in hypertension without pheo-chromocytoma and in hyperthyroidism. It was concluded that estimation of doma in urine could facilitate the diagnosis of pheochromocytoma, and that the catecholamines secreted from the tumors in the cases reported here are metabolized in the same fashion as exogenously administered catecholamines.
As the first step of epidemiological studies of regional differences in apoplectic death rate, practical surveys were performed on hypertension and other cardiovas-cular disturbances in 4 regions with different apoplectic death rates in Akita Pre-fecture in order to find out factors concerned in the differences, and the following results were obtained. 1) Average blood pressure and frequency of hypertension were higher in the regions where apoplectic death rate was higher, but the former were not always lower where the latter was lower. There was no parallelism be-tween them. 2) As to the relation between frequencies of cardiovascular distur-bances in hypertensive subjects and apoplectic death rates in the 4 regions, fre-quency of electrocardiographic abnormality did not show any parallelism with the death rates but did with the frequency of funduscopic abnormality, especially of high degree and proteinuria. 3) The living environment in the region with high apoplectic death rate was unhygienic, and it is therefore considered that this may increase the danger of bacterial infection, producing as the result complication of nephrosis or arteriosclerosis, thus exacerbating hypertension.