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Volume 163 (1991)
- Issue 4 Pages 233-
- Issue 3 Pages 149-
- Issue 2 Pages 77-
- Issue 1 Pages 1-
Volume 163, Issue 1
Displaying 1-7 of 7 articles from this issue
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HIROSHI TAKAHASHI, SHUICHI FUJITA, NOBUO TSUDA, FUMIAKI TEZUKA, HARUO ...1991 Volume 163 Issue 1 Pages 1-16
Published: 1991
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSTAKAHASHI, H., FUJITA, S., TSUDA, N., TEZUKA, F, and OKABE, H. Iron-Binding Proteins in Adenoid Cystic Carcinoma of Salivary Glands: An Immunohistochemical Study. Tohoku J. Exp. Med., 1991, 163 (1), 1-16 - An immunoperoxidase staining technique was used for detecting three major iron-binding proteins (transferrin, ferritin and lactoferrin) in 54 adenoid cystic carcinomas (ACCs) of major and minor salivary glands. Twenty-three normal salivary glands were investigated for comparison. Transferrin staining was detected mainly in the intercalated duct and serous acinar cells, and was found inconsistently in the myoepithelial cells surrounding normal ductules. Ferritin was always absent in the normal epithelial component of salivary gland. The presence of lactoferrin was demonstrated in the serous acinar cells and intercalated duct cells of normal salivary tissues. Five histological patterns were found in ACC, and for the cellular components of each, it was possible to establish a special immunohistochemical profile. Transferrin positivity was detected in the small angular cells of 25 cases (48%), in the duct luminal cells of 19 cases (37%) and in the hyalinized stroma of 4 cases (8%). Ferritin staining was positive in the small angular cells of 23 cases (44%) and in the duct luminal cells of 15 cases (29%). Lactoferrin was detected in only duct luminal cells of 38 ACCs (73%). The comparative immunohistochemical analysis between transferrin and ferritin showed a similar distribution in this carcinoma. On the basis of the immunohistochemical data, lactoferrin might be used as a marker of glandular differentiation.View full abstractDownload PDF (7035K) -
KIYOYASU FUKUSHIMA1991 Volume 163 Issue 1 Pages 17-30
Published: 1991
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSFUKUSHIMA, K. Expression of Lewisx, Sialylated Lewisx, Lewisa, and Sialylated Lewisa Antigens in Human Lung Carcinoma. Tohoku J. Exp. Med., 1991, 163 (1), 17-30 - One hundred and five cases of various human lung neoplasms were studied immunohistochemically using monoclonal antibodies recognizing the antigens, Lex, sialylated Lex (SLEX), Lea, and sialylated Lea (SLEA). Of the 92 lung cancers examined for antigen expression SLEX was detected in 57% (52 cases), Lex in 42% (39), SLEA in 36% (33), and Lea in 23% (21). None of these antigens were expressed in 9 tumor like lesions of the lung or the 4 other non-epithelial malignant lung tumors examined. Higher expression was seen in the 54 lung adenocarcinomas: SLEX in 72%, Lex in 48%, SLEA in 39%, and Lea in 24%. The type 2 carbohydrate antigens (SLEX and Lex) were more prevalent than the type 1 antigens (SLEA and Lea) in lung adenocarcinoma tissues. In adenocarcinomas, SLEX was expressed in 71% (10/14) of the Leb patients, and in 100% (5/5) of the Lea patients. Unexpectedly, SLEX was not detected in 4 out of 5 Lea-b- patients tested. This suggests that the expression of he Lex antigens and the Lea antigens are related in lung adenocarcinoma. These antigens were less expressed in other types of lung cancers. Tissue sections obtained serially showed heterogeneity in the expression of these antigens, as evidenced by the concurrent presence of both SLEX and SLEA. These results indicate that SLEX is a useful tumor-associated marker for lung adenocarcinomas, and that terminal fucosylation and sialylation may be activated heterogeneously in these lung cancers.View full abstractDownload PDF (8210K) -
YUTAKA TOKUE, SATORU SHOJI, KEN SATOH, AKIRA WATANABE, MASAKICHI MOTOM ...1991 Volume 163 Issue 1 Pages 31-37
Published: 1991
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSTOKUE, Y., SHOJI, S., SATOH, K., WATANABE, A. and MOTOMIYA, M. Detection of Methicillin-Resistant Staphylococcus aureus (MRSA) Using Polymerase Chain Reaction Amplification. Tohoku J. Exp. Med., 1991, 163 (1), 31-37 - Polymerase chain reaction (PCR) was applied for the detection of methicillin-resistant Staphylococcus aureus (MRSA). This procedure amplified a segment of MRSA-PBP (penicillin-binding protein) gene of DNA extract from the clinical isolates of S. aureus. A 1339-base-pair fragment of MRSA-PBP gene in DNA from S, aureus (MIC of methicillin, ≥16μg/ml) was amplified and detected by a specific oligonucleotide probe. Moreover, a 4.3kb HindIII fragment containing MRSA-PBP gene was detected by using the same oligonucleotide probe. On the other hand, no PCR-amplified product was detected in DNA from methicillin-sensitive S. aureus (MIC of methicillin, <16μg/ml).View full abstractDownload PDF (1276K) -
HIDEAKI KONO, YOUNG CHIN LIN, MAMORU YAMAGUCHI, FREDERICK P. ZUSPAN, N ...1991 Volume 163 Issue 1 Pages 39-45
Published: 1991
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKONO, H., LIN, Y.C., YAMAGUCHI, M., ZUSPAN, F.P., FURUHASHI, N., TAKAYAMA, K. and YAJIMA, A. Effects of Progesterone and Gossypol on Monoamine Oxidase Activity in Human Term Placental Explant. Tohoku J. Exp. Med., 1991, 163 (1), 39-45 - Human placental explant was cultured, using fresh full term placenta delivered by elective cesarean section (n=10), and monoamine oridase (MAO) activity was measured in the tissue by the spectrophotometric method. Placentae were obtained from patients without complications in pregnancy. Chorionic villi 2g was incubated for 3hr under the 95% air-5% CO2 humidified condition at 37°C. When progesterone was added in the media (100-500μg/ml), MAO activities significantly decreased, compared with the control. The gossypol (100μg/ml) treated group showed no inhibitory or stimulatory effects on MAO activities, compared with the control. However, progesterone (100-500μg/ml) and gossypol (100μg/ml) treated group showed the same value of MAO activities, compared with the control. Those results indicate that gossypol might block progesterone action which is inhibitory against MAO activity. The mechanism of pharmacological function of gossypol was discussed.View full abstractDownload PDF (620K) -
TOSHIHIKO MORIKAGE, YUTAKA MIZUSHIMA, SABURO YANO1991 Volume 163 Issue 1 Pages 47-57
Published: 1991
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSMORIKAGE, T., MIZUSHIMA, Y. and YANO, S. Prevention of Bacterial Infection and LPS-Induced Lethality by Interleukin-1α in Mice. Tohoku J. Exp Med., 1991, 163 (1), 47-57 - In order to make clear the characteristic biological properties of IL-1α, its protective capabilities on bacteria-induced and LPS-induced lethality were compared with other BRMs in BALB/c mice. Pretreatment with IL-1α (i.p., s.c.) or OK-432 (i.p.), could protect mice from the lethality of a K. pneumoniae infection (i.p.), and pretreatment with IL-1α or TNF α could protect mice from LPS (i.p.) lethality. IL-1α could protect mice from both bacteria- and LPS-induced lethality. There was no difference in serum corticosterone levels after the LPS injection between the IL-1α pretreated and untreated groups. However, a more rapid clearance of LPS from the serum and a stronger LPS-neutralizing activity in serum were observed with the LAL assay in IL-1α pretreated mice than in untreated mice. The enhanced LPS-clearance was suggested to be partly responsible for the increased protection against LPS-lethality. IL-1α was indicated to be a unique BRM and to become a useful tool for the prevention of life-threatening bacterial infections and subsequent endotoxin shock.View full abstractDownload PDF (1018K) -
HARUO NAKATSUKA, TAKAO WATANABE, SHIGERU HISAMICHI, HIROYUKI SHIMIZU, ...1991 Volume 163 Issue 1 Pages 59-71
Published: 1991
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNAKATSUKA, H., WATANABE, T., HISAMICHI, S., SHIMIZU, H., FUJISAKU, S., ICHINOWATARI, Y., KONNO, J., KURODA, S., HIRAI, J., IDA, Y., SUDA, S., KATO, K. and IKEDA, M. Different Rates of Acceptance of Health Examination among the People Under 2 Separate Health Insurance Programs in Japan. Tohoku J. Exp. Med., 1991, 163 (1), 59-71 - The entire population in Japan is covered by either workplace-based health insurance program or region-based health insurance program. While both programs were originally for curative medical care, only the former program has recently extended its financial support to preventive health examination. The attitude of the people toward health examination was studied in Miyagi prefecture in north-east Japan by questionnaires with more than 32, 000 respondents to examine whether the difference in health insurance reflect on the rates of acceptance of 4 health examination items (i.e., blood pressure measurement, chest x-ray taking, and 2 screening tests for stomach and cervical cancer). Throughout the 4 items, the rate of acceptance was higher among those who were covered by workplace-based health insurance than among those covered by regional health insurance. The difference is larger for the insured and less so for the family dependents of the insured. Further analysis for the preferred opportunities for health examination such as personal examination at clinics in contrast to region-based mass examination showed that the weight of clinics was quite large for the insured of national health insurance and their family dependents (i.e., 50% or even larger) but it was less so for the people covered by workplace-based insurance.View full abstractDownload PDF (1334K) -
HIROSHI SUZUKI, SETSUKO KITAOKA, TASUKE KONNO, TETSUO SATO1991 Volume 163 Issue 1 Pages 73-75
Published: 1991
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSUZUKI, H., KITAOKA, S., KONNO, T. and SATO, T. The Localization and Function of the Neutralizing Protein, VP4, in Human Rotavirus. Tohoku J. Exp. Med., 1991, 163 (1), 73-75 - The binding sites of a monoclonal antibody (mAb) to human rotavirus VP4 (K-1532) on the virion surface were examined with electron microscopy and the Markham photorotation method. The mAb, which had both hemagglutination inhibiting and neutralizing activities, appeared to bind to the outer margin of double-shelled particles of human rotavirus, especially to the parts overlaying the tubelike channels, which seemed to be the “cover lid” of the tubelike channels that extruded radially from the core region. This binding point may be a putative fusion site of the virus (Mackow et al. 1988) as well as a viral nucleus component ejection gate (Suzuki et al. 1986). Rotavirus particles treated with the mAb were shown to be capable of binding to MA104 cells by ultra-thin section examination. These observations suggest that neutralization via VP4 is related to virus uncoating but not to attachment of rotavirus.View full abstractDownload PDF (735K)
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