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Volume 185 (1998)
- Issue 4 Pages 227-
- Issue 3 Pages 161-
- Issue 2 Pages 79-
- Issue 1 Pages 1-
Volume 185, Issue 2
June
Displaying 1-9 of 9 articles from this issue
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Regular Contributions
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Yuichi Kasanuma, Chiho Watanabe, Choong-Yong Kim, Kun Yin, Hiroshi Sat ...1998 Volume 185 Issue 2 Pages 79-87
Published: 1998
Released on J-STAGE: December 13, 2005
JOURNAL FREE ACCESSTo determine whether mild and chronic heat stress leads to oxidative stress and to differenciate such effects of different exposure periods, we kept male ICR-mice at an ambient temperature of either 35°C or 25°C for 6 hours, 3 days, or 7 days and measured the concentrations of thiobarbituric acid reactive substances (TBARS), glutathione (GSH), selenium (Se), and glutathione peroxidase (GSH-Px) activities in the liver. Since the food consumption of the heat-exposed group was only half that of the control, we prepared pair-fed groups, which were kept at 25°C and whose food consumption were limited to those of the heat-exposed group for the 3-day and the 7-day exposure. TBARS concentrations of the liver was significantly higher in the heat group than the control after the 3-day exposure, while there was no significant difference among the groups after the 7-day exposure. There was no significant difference in GSH concentrations between the heat-exposed group and the control after the 7-day exposure, when the GSH concentration of the pair-fed group was significantly lower than that of the control. Hepatic cytosolic Se GSH-Px activity in the heat group was significantly less than that in the control group after the 6-hour exposure and it tended to be lower in the heat group than that of the control group after the 7-day exposure, while there was no difference in the total GSH-Px activity among the three groups. Our results showed that mild and chronic heat exposure may cause oxidative damage to organisms and that GSH-related anti-oxidative systems would play an important role to defensive reaction.View full abstractDownload PDF (570K) -
Fumiyoshi Ojima, Tatsuo Ido, Isao Kijima-Suda, Yoshito Nakagawa1998 Volume 185 Issue 2 Pages 89-100
Published: 1998
Released on J-STAGE: December 13, 2005
JOURNAL FREE ACCESSReactivity of the monoclonal antibody with the tumor markers is known to be different between cultured cells in vitro and transplanted tumors in vivo. The monoclonal antibody should be investigated regarding its specific accumulation in tumor-bearing mice for immunodetection or immunotherapy. We studied the biodistribution of radiolabeled monoclonal anti-GD3 antibody (IgM) in normal mice and nude mice bearing human melanoma xenografts. Tissue-to-blood distribution ratios of the antibody in the liver, spleen and kidney increased with time in both normal and melanoma-transplanted mice, but no significant changes were observed in other normal tissues up to 5 days after injection. Specific accumulation of the monoclonal anti-GD3 antibody in the grafted human melanoma (HMV-II) was observed 4 and 5 days after injection. On the other hand, no specific accumulation of standard murine IgM in the tissue of HMV-II was observed in mice bearing the HMV-II xenograft 5 days after injection. Because the tissue-to-blood ratio of the distribution in the tissue of HMV-II became larger than that of other tissues 4 and 5 days after administration, 4 days after the administration of the monoclonal anti-GD3 antibody were required for immunoscintigraphy. Accumulation of the monoclonal anti-GD3 antibody in other human melanomas (HMV-I, HMY-1 and SK-MEL188) inoculated into mice was also observed 4 days after the antibody administration. The monoclonal anti-GD3 antibody used in this study would be useful in immunodetection or immunotherapy.View full abstractDownload PDF (686K) -
Atilla Soran, Mükerrem Cete, Cavit Çöl1998 Volume 185 Issue 2 Pages 101-106
Published: 1998
Released on J-STAGE: December 13, 2005
JOURNAL FREE ACCESSIt is known that long-term administration of octreotide leads to changes in the histology of intraabdominal organs and plasma biochemical values. The purpose of the present study was to evaluate the histological effect of short-term octreotide administration on digestive organs in the experimentally induced pancreatitis by ligating pancreatic duct. The sham operation was performed on 20 rabbits in Groups 1 and 2. Acute pancreatitis was induced by pancreatic duct ligation in 20 rabbits in Groups 3 and 4. Octreotide was administered subcutaneously to the rabbits in Groups 2 and 4 at a dosage of 10 μg/kg/day for 7 days. The animals were sacrificed at the end of day 7, blood and tissue samples were collected. There was no histological changes in the stomach, duodenum, gallbladder, or small and large intestines of those group which received octreotide, while hepatic bile duct proliferation, bile duct epithelium proliferation, periportal inflammation and venous stasis were observed in liver histology. In conclusion, one-week octreotide administration in this experimental acute pancreatitis model was not associated with pathologic changes in digestive organs except liver.View full abstractDownload PDF (404K) -
Ting Chen, Hiroyuki Kato, Tsutomu Araki, Yasuto Itoyama, Kyuya Kogure1998 Volume 185 Issue 2 Pages 107-118
Published: 1998
Released on J-STAGE: December 13, 2005
JOURNAL FREE ACCESSCholinergic neurotransmission and protein kinase C (PKC) in the brain play important roles in the processes of cognitive function. In this study, we examined the effect of chronic treatment with rolipram, a 3', 5'-cyclic adenosine monophosphate (cyclic AMP)-selective phosphodiesterase inhibitor, on age-related changes in [3H]quinuclidinyl benzilate (QNB) and [3H]phorbol 12, 13-dibutyrate (PDBu) binding, which labeled brain muscarinic cholinergic receptors and PKC, respectively. Rolipram was administered per os to young (15 weeks old) and old (80 weeks old) Wistar rats at dosage of 0.01 mg/kg and 0.1 mg/kg once a day over 4 weeks. Then, quantitative in vitro autoradiography was performed. Control old rats showed elevations in [3H]PDBu binding in the hippocampus and the cerebellum compared to young rats, but [3H]QNB binding was largely unchanged. Chronic treatment of the old rats with the higher dose of rolipram led to reductions in [3H]QNB and [3H]PDBu binding in many brain regions. However, the same treatment of the young rats induced no or minimal effect. Thus, the response of the brain to rolipram was different between young and old rats. These results suggest that the cyclic AMP-selective phosphodiesterase system in the brain is modified during aging, modulating subsequently cholinergic neurotransmission and PKC activity exclusively in old rat brains.View full abstractDownload PDF (1182K) -
Tsutomu Akahane, Yoshimochi Kurokawa, Hiroshi Yaegashi, Susumu Satomi, ...1998 Volume 185 Issue 2 Pages 119-129
Published: 1998
Released on J-STAGE: December 13, 2005
JOURNAL FREE ACCESSLaser ablation has been employed as a therapeutic measure for chronic pulmonary emphysema. As yet, however, its effect is not understood on firm pathological basis. We aimed to study, both histopathologically and using Scanning Electric Micrscopy (SEM), the changes produced by irradiation with contact Neodymium-yttrium aluminum garnet laser (Nd: YAG laser) in rat lungs with experimentally induced emphysema. Emphysema was produced in 34 rats by instilling elastase via airways. Eight weeks after the instillation, the emphysematous left lung was irradiated under thoracotomy with contact Nd: YAG laser at a power of 5 watts. The animals were sacrificed in acute as well as chronic phase for histopathological observation of lung and scanning electron microscopy. Laser caused necrotic and inflammatory changes in the subpleural zone of lung. Immediately after irradiation, the alveolar septa were destroyed as visualized by SEM, only leaving the elastic skeleton. In a chronic phase, the necrotic zone was collapsed and replaced with a thick fibrous scar which seemed to serve more or less to keep the organ from being excessively inflated. In this model, irradiation induces subpleural dense scarring, which, by “encasing” an emphysematous lung, is expected to more or less normalize the excessive compliance.View full abstractDownload PDF (4149K) -
Tetsuaki Kawase, Hiroshi Hidaka, Masaki Ogura, Sho Hashimoto, Tomonori ...1998 Volume 185 Issue 2 Pages 131-137
Published: 1998
Released on J-STAGE: December 13, 2005
JOURNAL FREE ACCESSThe effects of the cut-off frequency of the filtered broadband stimuli on the human acoustic reflex (AR) were examined to observe the relation between the area of excitation in the cochlea and the AR response. The results obtained have indicated that all the input from the cochlear region does not equally contribute to trigger the AR equally; i.e., there is a lesser contribution from the frequency region below 700 Hz.View full abstractDownload PDF (385K) -
Yuji Otsuka, Hiroyuki Sakagami, Yuji Owada, Hisatake Kondo1998 Volume 185 Issue 2 Pages 139-146
Published: 1998
Released on J-STAGE: December 13, 2005
JOURNAL FREE ACCESSMammalian homologues for Drosophila trp are likely to be candidates for capacitative calcium entry channels. By in situ hybridization histochemistry, we have demonstrated that the mRNAs for four species of the mouse homologues (Mtrps-1, -3, -4, -6) were differentially expressed in the adult mouse brain. Mtrp-1 mRNA was expressed widely throughout the gray matters, while the expression for Mtrp-3 was dominant in the cerebellar Purkinje cells, the olfactory mitral cells and the striatal large-sized intrinsic neurons. Mtrp-4 mRNA was evident in the olfactory bulb, the septum, the hippocampal neuronal layers, and the cerebellar granule cell layer, while the expression for Mtrp-6 was rather confined to the dentate granule cell layer. Their differential localization suggests that the individual homologues exert their functions in region-specific and neuron-specific manners in the calcium signaling.View full abstractDownload PDF (3100K)
Case Report
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Kazunori Yoshida, Masahiro Arakawa, Shyuichi Ishida, Yasuhiko Sasaki1998 Volume 185 Issue 2 Pages 147-155
Published: 1998
Released on J-STAGE: December 13, 2005
JOURNAL FREE ACCESSHemolytic uremic syndrome (HUS) is characterized by microangiopathic hemolytic anemia, thrombocytopenia and acute renal failure. Most cases of HUS are characterized by a prodromal phase of diarrhea and melena, and affect mainly children. Here we report a unique case of adult-onset HUS that was associated with emphysematous cholecystitis and a liver abscess. The patient did not suffer from diarrhea or melena on admission, but abdominal CT scans revealed emphysematous cholecystitis and a liver abscess. Cholecystectomy was performed and the liver abscess was drained. Cultures of the bile and liver abscess contents were negative, but the serum samples had antibodies against Escherichia coli (E. coli) O157. The patient was anuric for 14 days, and underwent hemodialysis that was repeated 15 times and plasma exchanges 6 times. She recovered from acute renal failure but with inadequate urinary concentrating ability as a sequela. Histopathological examination of renal biopsy specimens on the 83rd hospital day revealed almost normal glomeruli and patchy atrophy of tubules with an increase of interstitium. This is a very rare case of HUS associated with emphysematous cholecystitis and a liver abscess successfully treated with aggressive supportive care. It is possible that an infection with verotoxin-producing E. coli O157 caused the disease.View full abstractDownload PDF (2057K)
Short Report
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Toshiaki Fushimi, Sanae Shimura, Satsuki Suzuki, Hiroki Saitoh, Hirosh ...1998 Volume 185 Issue 2 Pages 157-160
Published: 1998
Released on J-STAGE: December 13, 2005
JOURNAL FREE ACCESSWe examined the effect of dexamethasone on the gene expression and production of interleukin (IL)-13 by human peripheral blood mononuclear cells from healthy controls. The gene expression was assessed semiquantitatively by sequential transcription polymerase chain reaction and Southern blot analysis, and the production of this cytokine was assessed by enzyme-linked immunosorbent assay (ELISA). Dexamethasone suppressed IL-13 gene expression induced by stimulation with phytohemagglutinin and phorbol 12-myristate 13-acetate in a dose-dependent manner, with 96% suppression at 10−6 M, and also suppressed the increased production of IL-13. This is suggested to be one of the mechanisms by which glucocorticoids suppress allergic inflammation.View full abstractDownload PDF (320K)
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