Gross' reaction (GR) and Hayem's test (HT) were carried out on sera collected from 100 cattle, consisting of 56 brought to a slaughterhouse and 44 clinically healthy ones. The results obtained were compared with serum protein pictures determined by paper electrophoresis. The aim of this experiment was to clarify whether HT could be used as substitute for GR.
1. The 100 sera were divided into three groups, high-, normal-, and low-valued, according to the value of the serum protein fraction. Distribution of the degrees of response to GR and HT among these groups was studied byχ
2 examination. As a result, there were significant differences, at a level below 1 per cent, in albumin (A), total globulin (G), A/G ratio, and γ-globulin in both GR and HT. It was confirmed that these differences in distribution were induced, since there were many positive sera and a few negative sera both in the low albumin and low A/G ratio group and in the high total globulin and high γ-globulin group.
2. When the serum protein pictures and the results of GR and HT were compared, there were significant correlations, at a level below 1 per cent, in albumin, total globulin, A/G ratio, and γ-globulin in GR and HT, and a significant one, at a level below 5 per cent, in total protein content in GR alone.
3. The correlation coefficient between GR and HT was 0.589, which was significant at a level below 1 per cent.
4. The results of GR and HT were divided into two groups, negative and positive, and examined for the degree of agreement. It was found that there might be a very low probability for a chance to obtain more agreeable results than those of the present experiment. It was suggested that GR and HT might be essentially the same.
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