ヨ反応はグロス反応に代る簡易な臨床検査法であることが各方面で報じられ, 筆者らも多少修正した方法を考案し1961年以来乳牛に応用してきたが, 実用上明らかにすべき諸点が残されていることを感じ, 試験の結果つぎの知見を得た. 1) 任意のピペットの1滴量たはかなり大きなバラツキがあり, このため試薬対血清の量比が不定となり, ヨ反応の再現性が低下する. 1/3注射針程度のガラス毛細管をもちい振動を与えないよう静かに滴下すればこの欠陥はかなり防げる. 2) 同一ピペットで連続多数の検体を検査するとき, ピペット内で前後の血清の混合がおこり, ヨ反応に影響を与えることがある.1検体ごとにピペットをぬぐい去る注意が必要である. 3) 溶血血清ではヨ反応が強化し判定を誤りやすいので溶血防止に注意を要する. 4) 2重蔭酸加血漿ではヨ反応ははなはだ強く現われるので現行法では血清に代用できない. 5) 試薬は保存法さえよければ1年以上経てもヨ反応に大きな影響はない. 保存上注意すべき点はポリエチレン栓で密栓すること, 光線を避けること, 金属と接触させないことである. 6) 供試血清はとくに冷蔵は要しないが, 夏期4旧以内に供試すべきであろう.

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1. The most probable numbers (MPN) of Escherichia coil and Enterococcus in feces were determined in 10 head each of 9 animal species, including man, by the A. Y. I.-E. A. C. broth method. E. coli was found in dog, man, sheep, Japanese native ox, chicken, goat, guinea pig, rabbit, and dairy cow in the decreasing order of MPN. Enterococcus was detected in dog, sheep, chicken, man, goat, rabbit, guinea pig, Japanese native oxen, and dairy cow in the same order as above.
2. The number of enterococci was about 1/20 to 1/10 that of E. coli organisms in all the animals, except the chicken, in which it was almost equal to the latter.
3. When 487 samples of food were examined, 20.5 per cent of them was positive for E. coil and 13.7 per cent for Enterococcus. The resultant difference was significant at 1 per cent of a when examined by the X²-test.
4. The isolated organisms were classified into Streptococcus faecium (41.0%), Str. faecalis (36.4%), Str. durans (5.36%), Str. faecalis var. liquefaciens (4.6%), and atypical strains (12.64%).
5. Str. faecium was mostly found among the strains isolated from feces and Str. faecalis among those isolated from food.
6. The differences between the two bacterial species in the numbers of organisms isolated from “soft cream” and fresh milk were not significant at 5 per cent of α when examined by t-distribution.
These results seem to indicate that detection of enterococci, -when performed at the same time with that of E. coli organisms, may be useful as additional means for the determination of the degree of food contamination.

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A basic experiment was carried out to find a method of treatment of contaminated sera. In it, ultraviolet irradiation and β-propiolactone (PL) administration were examined for effect on canine serum contaminated artificially with infectious canine hepatitis (ICH) virus. When applied independently to the serum to such an extent as not injurious to the serum protein contained, neither of the two inactivating factors gave necessarily satisfactory results. Simultaneous application of the two factors, however, brought about good results within a short period of time, although the amount of ultraviolet irradiation used was less than half the amount applied alone and the concentration of PL one-trird to one-half that employed alone previously. When administered at such concentrations as these, PL exercised little influence upon neutralizing antibody in the serum and gave rise to no noticeable changes in the animal administered.

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アルサニル酸を, 飼料1kg当り100mg添加して, ニワトリヒナを9週令まで飼養した. アルサニル酸投与3週間で, ヒナの平均体重は, 対照区よりも重くなり, 以後9週令に至るまで, 添加区の平均体重値は, 常に対照区よりも高い値を示した. したがって, われわれの用いた実験条件では, ナルサニル酸は, ニワトリヒナの成長を促進する効果を宗したと結論できる.

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