Three lots of anti-canine distemper serum were prepared. They contained antibody at the titer of 558 (lot A), 1250 (lot B), and 13940 (lot C), respectively. Each of them was injected into dogs in a dose of 2 ml per kg of body weight and studied for the rise and fall of antibody in the dog body. As a result, antibody hardly existed at 10 to 14 days after injection with lot A. It showed a titer of 3 to 10 and 22 to 34 at 21 days after injection with lots B and C, respectively. The value of the highest antibody titer was proportional to the amount of antibody contained in antiserum. There was a tendency that the antibody titer decreased gradually, with its peak at 6 or 7 days after serum injection.
Some dogs were collected from places which were presumed to have been thickly contaminated with distemper virus. They were regarded as being at the early stage of infection with a distemper-like disease. Then they were injected with a serum-like product in a dose of 30, 000 per kg of body weight, together with some antibiotic. Among dogs injected with the product, penicillin, and str9ptomycin concurrently, many died after showing pneumonic symptoms, the rate of recovery being 40 per cent. Among those injected with the product and chloramphenicol, the same number of deaths occurred from among those manifesting pneumonic symptoms as those exhibiting encephalitic signs, the total rate of recovery being 40 per cent. The rate of recovery was 70 per cent among those injected with gamma-globulin preparation and chloramphenicol concurrently, indicating the synergistic action of the two agents.

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A 5 per cent solution of ferric chloride with addition of procaine hydrochloride was injected (0.3 to 2.0 cc per testicle) into the testicles of 8 pigs (2-3 months old) experimentally. In 7 pigs, the necrotic testicle began to discharge fluid from the hole of injection or skin wounds destroyed by the solution 7 to 10 days after the injection. The wounds were healed successfully after 14-21 days. In one case, however, a small mass was made by cicatrical tissue around the necrotic testicle with no discharge. The 8 pigs showed very good growth during 58 days after the injection, as well as 2 control pigs castrated surgically at the same time.
Using 34 young swine, a clinical examination was carried out by the same intratesticular injection. Twenty-five pigs began to discharge fluid from the necrotic testicle 7 to 14 days after the injection. Their wounds with discharge were healed successfully 14 to 30 days after the injection. The other 9 pigs were unsuccessful in castration. Of them, 8 pigs had a mass of cicatrical tissue around the necrotic testicle. The mass increased in size from little-finger's top to tennis-ball 30 days.after the injection, without disturbing growth of any pig. One pig, however, had a mass which became the size of child's head 141 days after the injection, growth being severely disturbed. This mass was formed by fibrinous edema around a necrotic focus which had been made by the solution injected into the outside of each testicle.
The rate of the successful intratesticular injection technique was 74 per cent. It seems lower than the surgical castration technique. That technique was so easy that a veterinarian could treat many young pigs in. a day. There was no bleeding nor infection after the injection of the solution. Accordingly the intratesticular injection technique for swine will become more practical than the surgical castration technique in future.

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Three lots of anti-canine distemper serum were prepared. They contained antibody at the titer of 558 (lot A), 1250 (lot B), and 13940 (lot C), respectively. Each of them was injected into dogs in a dose of 2 ml per kg of body weight and studied for the rise and fall of antibody in the dog body. As a result, antibody hardly existed at 10 to 14 days after injection with lot A. It showed a titer of 3 to 10 and 22 to 34 at 21 days after injection with lots B and C, respectively. The value of the highest antibody titer was proportional to the amount of antibody contained in antiserum. There was a tendency that the antibody titer decreased gradually, with its peak at 6 or 7 days after serum injection.
Some dogs were collected from places which were presumed to have been thickly contaminated with distemper virus. They were regarded as being at the early stage of infection with a distemper-like disease. Then they were injected with a serum-like product in a dose of 30, 000 per kg of body weight, together with some antibiotic. Among dogs injected with the product, penicillin, and str9ptomycin concurrently, many died after showing pneumonic symptoms, the rate of recovery being 40 per cent. Among those injected with the product and chloramphenicol, the same number of deaths occurred from among those manifesting pneumonic symptoms as those exhibiting encephalitic signs, the total rate of recovery being 40 per cent. The rate of recovery was 70 per cent among those injected with gamma-globulin preparation and chloramphenicol concurrently, indicating the synergistic action of the two agents.

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A survey was conducted in six fishing ports on the Japan Sea and three cities in Tottori Prefecture over a period of July, 1962, to March, 1965, to clarify the distribution of Vibrio Parahaemolyticus, an vibrio causing acute enteritis, in sea water and fish caught and on sale.
1. Vibriones were detected from 14 samples (16.7 per cent) of 84 of sea water collected from five ports. The positive samples were among those collected during a period of April to October.
2. When fish were examined at the fishing ports, vibriones were detected from 28 samples (12.5 per cent) of 225. The positive samples had been collected from four ports from April through October. As for fish on sale examined during a seven-month period ending in December, 1963, 4 samples (7.1 per cent) out of 56 were positive for isolation of vibriones. They had been collected from August to October.
3. A total of 65 strains of Vibrio Parahaemolyticus were isolated during this survey. Of them, 16 strains (24.6 per cent) belonged to biotype 1 and the other 49 strains to biotype 2.

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Infectious laryngotracheitis (ILT) was discovered in Osaka Prefecture in March, 1962. Since then, II4 outbreaks of ILT have been recognized by the authors till January, 1965.
A diagnosis of ILT was made when intranuclear inclusion bodies were found microscopically in smears of tracheal mucus, lesions were observed on the chorioallantoic membrane (CAM) of chicken embryo, or cytopathogenic changes and intranuclear inclusion bodies appeared in chicken kidney cell culture after inoculation with the field material to be tested. In some cases, neutralization tests were made between the virus detected from the trachea and the CAM inoculated with the field material and the immune serum to the ILT strain used to inoculate into chicken kidney cell culture.
In Osaka and Nara Prefectures, outbreaks of ILT have been found mostly in autumn and winter. On collective poultry farms, however, ILT has infected young chickens usually all the year round, but no outbreaks have been recognized among adult birds.

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放牧子牛における消化管内寄生線虫の実態調査を3カ年に亘り, 糞便検査を中心に実施した結果, 放牧子牛はいずれも線虫の高度な感染を受けていることが認められ, そのためにこうむる発育障害その他の被害は, かなり甚大なものであると推察された.
EPGの消長から, 放牧子牛が重度な感染を受ける時期は, 初山放牧時の6月～7月の梅雨期であることが推定され, この時期の衛生管理の重要性が指摘された.
また, この牧野における線虫の主力はCooperia属であり, ネグボンあるいはサイベンゾールによる駆虫によってEPGは著明に減少し, その効果は明瞭に認められた.
終りに本調査に当り終始ご懇得なご指導を賜った農林省家畜衛生試験場の渡辺昇蔵技官ならびに上野計技官に対し厚くお礼申し上げます.

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An epizootic broke out on a hog farm in Tokyo Prefecture in April, 1963. It was diagnosed as one of hog cholera, judging from clinical symptoms and autopsy findings obtained from some of the pigs involved. During this outbreak, 84 pigs exhibited no particular clinical signs among those which had been injected with crystalviolet inactivated vaccine (CVV) 1 to 2 months before the outbreak of hog cholera. When they were slaughtered in July of the same year, blood samples were collected from them and examined for the presence of hog cholera virusneutralizing antibody by means of the END (exaltation of Newcastle disease virus) method. Fifty-nine pigs examined gave the following neutralizing antibody titers, as expressed by the serum dilution:≤ 20 in one head (1.7 per cent), 40 in one head, 160 in six head (10.1 per cent), 640 in thirteen head (22.0 per cent), and 1280 in thirty-eight head (64.4 per cent). Judging from these high antibody titers, it was confirmed that the epizootic was that of hog cholera.
Furthermore, pigs inoculated with CVV were subjected experimentally to challenge inoculation with a high virulent strain of hog cholera virus. They withstood the challenge inoculation, usually showing a mild reaction, if any. After this inoculation, the neutralizing antibody titer increased rapidly and remarkably in them. From these findings, it is presumed that those pigs which resisted infection, without manifesting any clinical signs, during the present epizootic might have been infected with hog cholera, although they had been inoculated previously with CVV.

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