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Volume 150 (1986)
- Issue 4 Pages 365-
- Issue 3 Pages 231-
- Issue 2 Pages 103-
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Volume 150, Issue 3
Displaying 1-16 of 16 articles from this issue
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MASAO KOBARI, HIROTAKE HISANO, SEIKI MATSUNO, TOSHIO SATO, MIKIO KAN, ...1986 Volume 150 Issue 3 Pages 231-248
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKOBARI, M., HISANO, H., MATSUNO, S., SATO, T., KAN, M. and TACHIBANA, T. Establishment of Six Human Pancreatic Cancer Cell Lines and Their Sensitivities to Anti-Tumor Drugs. Tohoku J. exp. Med., 1986, 150 (3), 231-248-Six human pancreatic cancer cell lines PK-1, -8, -9, -12, -14 and -16, were established. They originated from either primary pancreatic cancer biopsy or liver metastasis biopsy, or xenografts of these biopsy specimens in athymic nude mice. The primary tumors were all well differentiated adenocarcinoma of pancreatic duct origin. The six established PK cell lines were all CEA positive and had tumorigenicity in athymic nude mice. Morphology of the xenografted tumors was closely similar to that of the original tumor. PK cells grew slowly with the doubling time of 41.3 to 82hr and showed aneuploid chromosome pattern. High levels of glucose-6-phosphate dehydrogenase (G6PDH) and lactic dehydrogenase (LDH) were found in each cell extract. Trypsin was not detected in cell extracts except PK-8 and PK-9. In chemosensitivity test, all of PK cell lines were sensitive to aclacinomycin A (ACM), and PK-1 and PK-8 were sensitive to 5-Fluorouracil (5-Fu) at concentrations of 0.02μg/ml, ACM and 1μg/ml, 5-Fu, when the drugs were used for over 48hr. At higher concentrations, they showed time independent sensitivity to mitomycin C (MMC). PK-9 was resistant to 5-Fu and MMC.View full abstractDownload PDF (4652K) -
KYOSUKE MASAKI, YUTAKA WAKO, TAKASHI URANO, SHUICHI KIMURA1986 Volume 150 Issue 3 Pages 249-257
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSMASAKI, K., WAKO, Y., URANO, T. and KIMURA, S. Purification of Rat Retinol-Binding Protein Using Blue Sepharose CL-6B. Tohoku J. exp. Med., 1986, 150 (3), 249-257-Studies were conducted to isolate rat plasma retinol-binding protein, the specific transport protein for vitamin A in the rat. Retinol-binding protein was isolated from rat plasma by a new simple procedure using Blue Sepharose CL-6B, and highly purified retinol-binding protein was obtained. This procedure included DEAE-cellulose chromatography at pH 6.0, Sephadex G-75 gel filtration in the presence of 3.0M urea, Blue Sepharose CL-6B affinity chromatography at pH 7.0 and finally Sephadex G-100 gel filtration at pH 7.4. The third step completely accomplished the dissociation and separation of retinon-binding protein from its complex with prealbumin and plasma albumin. This procedure is a significant improvement over previously published procedures, in which sample recycling and preparative polyacrylamide gel electrophoresis are necessary. The molecular weight, electrophoresic behaviour, ultraviolet and fluorescence spectra of the retinol-binding protein were similar to those appearing in other reports.View full abstractDownload PDF (977K) -
HIDETADA SASAKI, FREDRIC G. HOPPIN, Jr., TAMOTSU TAKISHIMA1986 Volume 150 Issue 3 Pages 259-271
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSASAKI, H., HOPPIN, F.G., Jr. and TAKISHIMA, T. Effect of Duration of Stimulation on Mechanical Properties of Trachealis Muscle. Tohoku J. exp. Med., 1986, 150 (3), 259-271-We have studied the effect of duration of stimulation on the dynamic behavior of isolated dog trachealis muscle topically contracted by alternating current or carbachol. With the muscle contracted quick stretch and quick release maneuvers were performed. The force-length curves during the quick maneuvers were similar for both methods of stimulation and regardless of the duration of stimulation. This behavior is consistent with an undamped passive series elastic component. The plateaux of force preceding these quick maneuvers were also similar. Isometric stress relaxations after quick stretch were similar. However, after quick release, isometric stress recovery and isotonic shortening velocity were more rapid with alternating current stimulation than with carbachol, and both isometric stress recovery and isotonic shortening velocity decreased with increasing duration of stimulation by either means of stimulation. We conclude that the difference in velocity of shortening is not due to the different method of stimulation but rather to the duration of stimulation. Active respiratory smooth muscle may show different dynamic properties depending on the duration of stimulation but not on the differences in means of stimulation.View full abstractDownload PDF (1303K) -
KUNITOSHI NAKAGAWA, SHUJI MATSUBARA, KIYOAKI OUCHI, YASUO OWADA, YOSHI ...1986 Volume 150 Issue 3 Pages 273-280
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNAKAGAWA, K., MATSUBARA, S., OUCHI, K., OWADA, Y. and YAJIMA, Y. Endotoxemia after Abdominal Surgery. Tohoku J. exp. Med., 1986, 150 (3), 273-280-The blood level of endotoxin after operations in patients with digestive diseases, mainly liver cirrhosis and obstructive jaundice, and the complications most likely related to the presence of endotoxemia were investigated. Twenty-seven patients without either liver cirrhosis or obstructive jaundice showed a minimal elevation of the endotoxin level in blood, as shown by 6.1±3.9 (mean± S.E.) pg/ml at the first postoperative day and there was only one anastomotic leakage. On the other hand, 18 patients with liver cirrhosis showed a notable and persistent endotoxemia after surgery. The cirrhotic patients who especially underwent splenectomy and hepatectomy showed marked elevations of endotoxin level at the first postoperative day, with values of 151.0±46.1pg/ml and 101.3± 36.2pg/ml, respectively, and one of these patients died of hepatic failure. Thirteen patients with obstructive jaundice developed endotoxemia evidenced by the value of 21.6±4.8pg/ml at the first day after surgery. Among these patients, two had gastrointestinal bleeding and one developed disseminated intravascular coagulation (DIC). The markedly high and persistent levels of endotoxin in patients with liver cirrhosis or obstructive jaundice may be possibly related with the development of multiple organ failure (MOF).View full abstractDownload PDF (680K) -
MASAHIKO ITO, EIKO ISHIDA, FUMINORI TANABE, NORIO MORI, SHIRO SHIGETA1986 Volume 150 Issue 3 Pages 281-286
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSITO, M., ISHIDA, E., TANABE, F., MORI, N. and SHIGETA, S. Inhibitory Effect of Liposome-Encapsulated Penicillin G on Growth of Listeria monocytogenes in Mouse Macrophages. Tohoku J. exp. Med., 1986, 150 (3), 281-286-Administration of penicillin G encapsulated in liposomes inhibited the proliferation of Listeria which infected mouse intraperitoneal resident macrophages whereas free penicillin G and/or liposomes did not. This result suggests that administration of antibiotics encapsulated in liposomes is an effective treatment for the intracellular infection.View full abstractDownload PDF (527K) -
SHIGERU ASAKI, YOSHIO GOTO, TSUNEO TANAKA1986 Volume 150 Issue 3 Pages 287-297
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSASAKI, S., GOTO, Y. and TANAKA, T. Utility of BID Administration of Cimetidine for Gastric Ulcer: A Comparison with QID Administration in a Double Blind Manner. Tohoku J. exp. Med., 1986, 150 (3), 287-297- The clinical efficacy of cimetidine for gastric ulcer was compared between the standard administration methods in Japan; 200mg four times daily and 400mg twice daily after breakfast and at bedtime in a double blind manner. It was found that the results obtained by 200mg twice-daily administration were almost comparable to those obtained by the four-times-daily administration without statistically significant difference for healing rate, final global improvement in subjective and objective symptoms, incidence of adverse reactions and effects on laboratory tests. These findings resulted in that the twice-daily administration of cimetidine provides convenience for patients.View full abstractDownload PDF (1046K) -
TOMOHARU AKIBA, MASARU YOSHIKAWA, SHINSUKE OTAKI, YOKIO KOBAYASHI, MIT ...1986 Volume 150 Issue 3 Pages 299-307
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSAKIBA, T., YOSHIKAWA, M., OTAKI, S., KOBAYASHI, Y., NAKASATO, M., SUZUKI, H. and SATO, T. Angiocardiographic Estimation of Left Ventricular Mass in Normal Infants and Children. Tohoku J. exp. Med., 1986, 150 (3), 299-307-Left ventricular mass data were obtained from biplane cineangiocardiograms in 37 normal infants and children. Left ventricular mass calculated by using thickness of anterior wall (left ventricular mass-anterior) and posterior wall (left ventricular mass-posterior) of the free left ventricular wall were studied in relation to body surface area and left ventricular end-diastolic volume. Both of left ventricular mass-anterior and mass-posterior were expressed as a function of body surface area with exponential relationship ; left ventricular mass-anterior=68.5 (body surface area)1.46g (r=0.98, p<0.01), left ventricular mass-posterior=84.6 (body surface area)1.54g (r-0.97, p<0.01). Left ventricular mass-anterior/left ventricular end-diastolic volume averaged 0.73±0.01g/ml, and left ventricular mass-posterior/left ventricular end-diastolic volume 0.89±0.02g/ml, respectively. Thus, left ventricular mass can be predicted from body surface area by using the exponential equations. In addition, normal values for left ventricular mass- posterior must be discriminated from those for left ventricular mass-anterior.View full abstractDownload PDF (607K) -
ISAO OHYA, MINEO IWASA, HIDEOU KOMORIYA, YASUO BUNAI, KAORU SAGISAKA1986 Volume 150 Issue 3 Pages 309-315
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOHYA, I., IWASA, M., KOMORIYA, H., BUNAI, Y. and SAGISAKA, K. Identification of Human Saliva by Antisera to α-Amylase in Human Salivary Glands. Tohoku J. exp. Med., 1986, 150 (3), 309-315-Amylase activities were detected significantly in saliva from human, macaques and rodents and slightly in the vegetable and fruit extracts. Dried stains on filter paper prepared from human and mammalian saliva, and the vegetable and fruit extracts were subjected to starch-iodine test and blue starch polymer-agar plate test. Both tests showed strong positive reactions with the macaque and rodent saliva stains as well as human, but the vegetable and fruit stains showed clear positive reactions only in the starch-iodine test. The results suggest that these tests are not specific for human saliva and that for screening test of saliva stains, blue starch polymer-agar plate test is more suitable than starch-iodine test. Rabbit antisera against α-amylase isolated from the human submaxillary glands were prepared. In double immunodiffusion test with the human and mammalian saliva, human and macaque saliva produced precipitation lines. Human saliva gave patterns of partial identity with saliva from Japanese monkey and crab-eating monkey and these two macaque saliva gave total identity with each other. Anti-α-amylase sera absorbed with Japanese monkey saliva reacted only with human saliva. This suggested that the anti-α-amylase sera absorbed with macaque saliva make it possible to identify human saliva stain.View full abstractDownload PDF (3153K) -
YOSHIAKI YAJIMA, ICHIRO FUKUDA, MASAO OTSUKI, HIROSHI SUZUKI, YOSHIO G ...1986 Volume 150 Issue 3 Pages 317-325
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSYAJIMA, Y., FUKUDA, I., OTSUKI, M., SUZUKI, H. and GOTO, Y. Stability of Endotoxin Detected in Human Plasma against Endotoxin-Inactivating Factor (EIF): Quantitative Analysis of EIF Using Chromogenic Endotoxin Assay. Tohoku J. exp. Med., 1986, 150 (3), 317-325-Using a quantitative blood endotoxin assay utilizing chromogenic substrate coupled with perchloric acid pretreatment (PCA-LCT), we showed the presence of endotoxin-inactivating factor (EIF) in human plasma in vitro. EIF activity inactivated added endotoxin to about 10-4 of the initial level within 20min, followed by a stable phase where the residual endotoxin became resistant to EIF and was not further inactivated. The residual endotoxin may represent the endotoxin in patient plasma which is also EIF resistant. We postulate that endotoxin, upon entering the blood, is rapidly inactivated by chemical modification of its active site, lipid A, through EIF. Subsequently, inactivated endotoxin, mainly consisting of polysaccharide, is gradually removed from circulation by endocytosis in the reticuloendothelial system.View full abstractDownload PDF (714K) -
TETSU YAMAMOTO, SANYA SAKADA1986 Volume 150 Issue 3 Pages 327-336
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSYAMAMOTO, T. and SAKADA, S. Sensory Innervation of Gingival and Alveolar Mucosa of the House Musk Shrew (Suncus murinus). Tohoku J. exp. Med., 1986, 150 (3), 327-336-The lingual gingival and the alveolar mucosa of mandible of the house musk shrew (Suncus murinus) were stained by methylene blue vital staining or osmic acid staining, and mounted as whole thickness preparations. The sensory innervation and the distribution of sensory receptors were investigated with a light microscope. The nerve fibers supplying these regions derive from the sublingual nerve, which ascend in the mucosa as they branch out. Sensory receptors found in the present study are of four kinds ; free nerve endings, bush-like nerve endings, Merkel cell-neurite complexes and encapsulated corpuscles. The Merkel cell-neurite complexes were scarce and localized in the upper margin of gingival mucosa. The bush-like nerve endings were distributed preferentially in the alveolar mucosa, in which their maximum density was 9-23per mm2. Among the organized receptors, the encapsulated corpuscles appeared most frequently throughout the mucosal area investigated, and their maximum density amounted to 27-56per mm2 in the gingival mucosa. These corpuscles were relatively small and poorly differentiated. Although the bush-like nerve endings and the encapsulated corpuscles were fewer in the third molar region, there was no obvious regional difference in their distribution densities from the premolar region to the second molar region.View full abstractDownload PDF (2533K) -
REIKO KUMAGAI, HISAE NIITSU, IKUYO KATSURA, NORI NAKAYASHIKI, SYUSAKU ...1986 Volume 150 Issue 3 Pages 337-343
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKUMAGAI, R., NIITSU, H., KATSURA, I., NAKAYASHIKI, N. and KATSURA, S.A Case of a Newborn Infant with Hb M Iwate. Tohoku J. exp. Med., 1986, 150 (3), 337-343-Hemoglobin of a newborn infant who was suspected to hereditarily have Hb M Iwate was examined. The infant hemolysate was separated into five fractions by column chromatography on Amberlite CG-50, and two of these fractions showed absorption spectra corresponding with that of Hb M Iwate. Five bands were found after the isoelectric focusing of the hemolysate, and two of these bands were brown. The two Hb M fractions obtained by column chromatography was focused to the positions of the brown bands. One of these Hbs M corresponded with Hb M Iwate (αM2β2) from an adult carrier of this trait, but the other was not found in adult hemolysates. The latter species of Hb M was shown to be composed of the abnormal α chain and the normal γ chain (αM2γ2) by chain analysis, and was assumed to be specific for infants. A quantitative estimation of the hemoglobins in the infant hemolysate showed that there was no difference between the relative quantities of the fetal and adult forms of Hb M Iwate.View full abstractDownload PDF (997K) -
KAORU SAGISAKA, MASAYASU NOGUCHI, MASATO FUNAYAMA, YASUHIRO AOKI1986 Volume 150 Issue 3 Pages 345-351
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSAGISAKA, K., NOGUCHI, M., FUNAYAMA, M. and AOKI, Y. Heat and Chemical Stability of Blood Group-Specific Immunoglobulins in the State of Immunecomplex. Tohoku J. exp. Med., 1986, 150 (3), 345-351-Immune agglutinin or precipitin loses its reactivity, with the corresponding antigen by heating or some chemicals. A. Se group saliva or M group blood stain was sensitized with human IgM anti-A or rabbit IgG anti-M serum, respectively. After being washed with saline, the stains were heated or treated with formalin or 2-mercaptoethanol (2-ME) of various concentrations. The immunoreactivity of the antibodies in the state of immunecomplex was examined by the mixed agglutination or elution test, indicating IgM and IgG antibodies in the state of immunecomplex were proof against heating up to 150°C for 10min and against formalin and 2-ME of high concentrations. It is considered that IgM and IgG acquire a new property of heat and chemical stability by formation of immunecomplex although they are substantially heat and chemical labile.View full abstractDownload PDF (848K) -
HIRONAKA KAWASAKI, YASUHITO YAMANISHI, MARIKO MIYAKE, TETSUO MURA, SHI ...1986 Volume 150 Issue 3 Pages 353-357
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKAWASAKI, H., YAMANISHI, Y., MIYAKE, M., MURA, T. and IKAWA, S. Age-and Sex-Related Profiles of Serum Primary and Total Bile Acids in Infants, Children and Adults. Tohoku J. exp. Med., 1986, 150 (3), 353-357-The relation of age and sex to serum primary (PBA) and total bile acid (TBA) concentrations was evaluated by an enzymatic fluorometric microassay in healthy infants, children and adults. TBA concentrations were the highest in the 6-day-old group and 1-month-old group and seemed to switch to almost normal adult levels by the age of 4-6 years, which persisted throughout life, while PBA concentrations were predominant over a period of 3 days to 3 months after birth. No sex-related differences were observed from neonates to very old persons for any of serum bile acids. These results show that the bile acid metabolism in the liver and the enterohepatic circulation of bile acids are usually matured in infancy and that aging and sex may insignificantly affect serum bile acid metabolic profiles.View full abstractDownload PDF (499K) -
NORI KOMATSU, AKIRA KIDO, MASAKAZU OYA1986 Volume 150 Issue 3 Pages 359-360
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKOMATSU, N., KIDO, A. and OYA, M. An Electrophoretic Slow PGM1 Variant Detected in a Yamanashi Population. Tohoku J. exp. Med., 1986, 150 (3), 359-360 -Using starch gel electrophoresis, a slow variant PGM1 6NGS2-1 (W23-1A) was detected among 749 residents of Yamanashi Prefecture. The isoelectric focusing pattern of this type was demonstrated, which would serve as a reference in further population studies.View full abstractDownload PDF (504K) -
TADASHI KANOH, TATSUHARU OHNO, NORIMUNE FUNAKOSHI, HIROSHI FUJII, TAKA ...1986 Volume 150 Issue 3 Pages 361-362
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKANOH, T., OHNO, T., FUNAKOSHI, N., FUJII, H. and OKADA, T. LAV/HTLV-III Antibodies in Patients Treated with Intravenous Gammaglobulins. Tohoku J. exp. Med., 1986, 150 (3), 361-362-The test sera from 50 patients treated with intravenous gammaglobulins (IVG) were examined for LAV/HTLV-III antibody by ELISA tests in order to evaluate the risk of transmission of LAV/HTLV-III virus via administration of IVG. The three ELISA-positive sera were negative for the antibody by both indirect immunofluorescence assay (IFA) and Western blot assay (WBA). Thus, all of the test sera were negative for LAV/HTLV-III antibody. Two of the ELISA-positive/IFA-negative/WBA-negative samples had HLA-DR4 antibodies causing positive ELISA results.View full abstractDownload PDF (236K) -
MASACHIKA SENBA, NORIKO FUKUSHIMA, TAKAYOSHI TODA1986 Volume 150 Issue 3 Pages 363-364
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSENBA, M., FUKUSHIMA, N. and TODA, T. A Rapid Silver Staining Method for Identification of Mycobacterium leprae in Histologic Sections. Tohoku J. exp. Med., 1986, 150 (3), 363-364-A few methods have been reported for the purpose of staining Mycobacterium leprae in paraffin sections, including Fite oil fuchsin method, auramine-rhodamine method, and Blanco-Fite silver method. Among these staining techniques, Fite oil fuchsin method and auramine-rhodamine method are popular. However, the Blanco-Fite silver method takes approximately 20 days. Therefore, we developed a new procedure for rapid identification of M. leprae in paraffin sections using another silver solution and found that the procedure gave stable and satisfactory results. This new method has proved superior to others in demonstrating a reliable staining for M. leprae.View full abstractDownload PDF (443K)
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