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Volume 165 (1991)
- Issue 4 Pages 247-
- Issue 3 Pages 165-
- Issue 2 Pages 67-
- Issue 1 Pages 1-
Volume 165, Issue 2
Displaying 1-10 of 10 articles from this issue
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RITSUO USUI, HIDEO ISE, OSAMU KITAYAMA, AKIHITO MORIYASU, YUTAKA ABE, ...1991 Volume 165 Issue 2 Pages 67-77
Published: 1991
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSUSUI, R., ISE, H., KITAYAMA, O., MORIYASU, A., ABE, Y., SATOH, S., INOUE, H., HAYASAKA, H., HIRAMA, Y., HONDA, H., SUZUKI, N. and MATSUNO, S. Bilirubin Conjugation and Biliary Bilirubin Excretion after Intravenous Bilirubin Injection in Dogs. Tohoku J. Exp. Med., 1991, 165 (2), 67-77, -This study was undertaken to investigate the bilirubin clearance, bilirubin conjugation and biliary bilirubin excretion after an intravenous single bilirubin injection (5mg/kg or 10mg/kg) in 10 mongrel dogs. Serum total bilirubin concentration increased after the bilirubin injection, and then it immediately decreased. Biliary excretion of loaded bilirubin induced a rapid increase of the bile total bilirubin concentration and bilirubin-monoconjugate. In the 10mg/kg group, the increase was larger than in the 5mg/kg group and remained high up to 4hr after injection, whereas in the 5mg/kg group these bile components tended to decrease and returned to the preinjection levels within 4hr. The total bile acid concentration in the bile remained unchanged in both groups. The bile flow did not change in the 5mg/kg group, but in the 10mg/kg group it decreased after bilirubin loading. Significantly positive correlations were observed between total bilirubin and bilirubin monoconjugate concentrations in bile both before and 1hr after injection, but the slopes of regression lines were markedly different from each other. These results suggest that a bilirubin load surpassing the conjugating capacity of the hepatocytes increases bile bilirubin monoconjugate and also enhances the conjugating activity in the liver.View full abstractDownload PDF (942K) -
NOBUAKI FURUHASHI, MASASI TSUJIEI, HIROSI KIMURA, AKIRA YAJIMA, HIROYA ...1991 Volume 165 Issue 2 Pages 79-86
Published: 1991
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSFURUHASHI, N., TSUJIEI, M., KIMURA, H., YAJIMA, A., NAGAE, H. and KIMURA, C. Maternal and Fetal Atrial Natriuretic Peptide Levels, Maternal Plasma Renin Activity, Angiotensin II, Prostacyclin and Thromboxane A2 Levels in Normal and Preeclamptic Pregnancies. Tohoku J. Exp. Med., 1991, 165 (2), 79-86-To clarify the possible role of elevated atrial natriuretic peptide (ANP) in the pathophysiology of preeclapmsia, we measured ANP, renin activity (PRA), angiotensin II (Ang II), TXB2 (a stable metabolite of TXA2) and 6-keto-PGF1α (a stable end product of PGI2) concentrations in the plasma of 19 normal pregnant women and 35 severe preeclamptic patients at term. Plasma ANP levels in the preeclamptic patients (n=35, 71.5±3.8pg/ml, mean±S.E.) and also umbilical plasma ANP (n=35, 83.0±4.2pg/ml) were significantly (p<0.01) higher than those of normal pregnant women plasma (n=19, 58.7±3.7pg/ml) and umbilical plasma (n=19, 47.6±4.7pg/ml ). There was a significant (p<0.01) positive correlation between maternal ANP levels and fetal ANP levels (n=54, r=0.44). Plasma PRA and 6-keto-PGF1α levels in preeclampsia were significantly (p<0.05) lower than those of normal pregnancy. The ratio of 6-keto-PGF1α/TXB2 in preeclampsia was significantly (p<0.01) lower than that of normal pregnancy as we reported previously. There was no significant correlation between plasma ANP level and plasma PRA, Ang II, plasma TXB2 and 6-keto-PGF1α concentrations. Moreover there was no significant correlation between plasma ANP level and the severity of preeclampsia. These data suggest the possibility of a transplacental crossing of ANP secreted by feto-placental unit, which might be, at least in part, responsible for the high ANP levels observed in preeclampsia. The ANP in preeclampsia is not related directly to hypertension, but it may play a substantial role in the regulation or normalization of blood volume and vascular reactivity.View full abstractDownload PDF (784K) -
YASUO HORIE, MITSURO CHIBA, MASAHIRO IIZUKA, OSAMU MASAMUNE1991 Volume 165 Issue 2 Pages 87-97
Published: 1991
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSHORIE, Y., CHIBA, M., IIZUKA, M. and MASAMUNE, O. Class II (HLA-DR, HLA-DP, and HLA-DQ) Antigens on Colonic Epithelia in Ulcerative Colitis: A Comparison between Uneventful and Intractable Cases. Tohoku J. Exp. Med., 1991, 165 (2), 87-97-Class II antigens on colonic epithelia involved in ulcerative colitis (UC) with uneventful or intractable courses were investigated using an immunoperoxidase method. Twenty normal colonic mucosa served as normal controls. Nineteen specimens from 14 uneventful cases and 23 specimens from 12 intractable cases were studied. Normal colonic epithelia did not express class II antigens. In UC, there were no differences between uneventful cases and intractable cases in the frequencies of class II antigen expression, the distribution of class II antigen expression ratio, or the mutual relationship of the extent of expression of the three class II antigens on colonic epithelia. However, while the extent of expression of HLA-DR and HLA-DP antigens on the epithelia was positively correlated with the degree of inflammation in uneventful cases, there was no such correlation in intractable cases. These observations may suggest that the induction mechanisms of class II antigens on colonic epithelia in UC differ in uneventful and intractable cases.View full abstractDownload PDF (3273K) -
KAZUHIKO HOSHI, SUSUMU TSUKIKAWA, AKIRA SATO1991 Volume 165 Issue 2 Pages 99-104
Published: 1991
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSHOSHI, K., TSUKIKAWA, S. and SATO, A. Importance of Ca2+, K+ and Glucose in the Medium for Sperm Penetration through the Human Zona Pellucida. Tohoku J. Exp. Med., 1991, 165 (2), 99-104-When salt-stored human oocytes were inseminated in the regular mBWW medium and examined 6hr later, the zonae of almost all of the oocytes were penetrated by spermatozoa. In contrast, none of zonae were penetrated when the oocytes were inseminated in either Ca2+-free, K+-free or glucose-free medium. The failure of zona penetration by spermatozoa in Ca2+-free and K+-free media is likely to be due to the inhibition of the acrosome reaction. Spermatozoa were unable to exhibit hyperactivated motility in glucose-free medium, suggesting that the failure of zona penetration in this medium is due, at least in part, to the inhibition of hyperactivated motility of spermatozoa.View full abstractDownload PDF (550K) -
TOKIO NAKANE, YASUYUKI FURUKAWA, SHIGETOSHI CHIBA1991 Volume 165 Issue 2 Pages 105-113
Published: 1991
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNAKANE, T., FURUKAWA, Y. and CHIBA, S. Anti-Muscarinic Effect of Alinidine on Acetylcholine-Induced Vasodilation in Isolated and Perfused Dog Coronary Arteries. Tohoku J. Exp. Med., 1991, 165 (2), 105-113-The effect of alinidine, a bradycardic agent, on the vasodilator responses to acetylcholine was examined in isolated and perfused dog coronary arteries. Single injections of acetylcholine (10-12-10-6mol) and carbachol (10-10-10-6mol) produced dose-dependent vasodilations. The endothelial removal by a bolus injection of saponin (1mg) inhibited those vasodilations. Alinidine (10-6M) shifted the dose-response curves of acetylcholine and carbachol to the right, but it did not affect those for isosorbide dinitrate, isoproterenol and adenosine. The rank order of potency of muscarinic antagonists for inhibiting the acetylcholine-induced vasodilation was 4-DAMP≥atropine>AF-DX 116≥pirenzepine>alinidine. Alinidine was approximately 100 times less potent than atropine. Single injection of alinidine (10-8-10-6mol) dilated the dog coronary artery in a dose-related manner. The vasodilation was not affected by the pretreatment with phentolamine (10-6M), pindolol (10-6M), atropine (10-6M), chlorpheniramine (10-6M), cimetidine (10-6 M) or methysergide (10-6M). These results suggest that alinidine has a weak anti-muscarinic effect on the endothelium-dependent vasodilation of the dog coronary artery.View full abstractDownload PDF (892K) -
MASAHIRO OHTA, TOKIHISA KIMURA, KOZO OTA, MASARU SHOJI, MINORU INOUE, ...1991 Volume 165 Issue 2 Pages 115-129
Published: 1991
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOHTA, M., KIMURA, T., OTA, K., SHOJI, M., INOUE, M., SATO, K., YAMAMOTO, T. and YOSHINAGA, K. Effect of Vasopressin on Cardiovascular and Renal Functions and ANP Release under Plasma Volume Expansion. Tohoku J. Exp. Med., 1991, 165 (2), 115-129-To assess the mechanisms whereby arginine vasopressin (AVP) increases atrial natriuretic peptide (ANP) release and to examine whether AVP-mediated ANP release affects the effect of AVP on the cardiovascular and renal function, AVP was infused continuously at a rate of 20ng•kg-1 min-1 for 75min following 200ng/kg bolus injection under stepwise increases in plasma volume in anesthetized dogs. Moreover, the effect of an AVP antagonist, a V1 blocker, 1-(β-mercapto-β, β-cyclopentamethylenepropionic acid)-2-(o-methyl)tyrosine AVP (TMeAVP), on these parameters was also investigated. In the control study, saline alone was infused. AVP infusion increased total peripheral resistance (TPR), pulmonary capillary wedge pressure (PCWP), and plasma ANP, but decreased cardiac index (CI) and heart rate (HR) without increases in mean arterial blood pressure (MABP). Stepwise rises in plasma volume further increased plasma ANP, CVP, CI and PCWP, but gradually decreased TPR. TMeAVP curtailed AVP-induced increases in TPR and plasma ANP as well as decreases in CI and HR. The replacement of ANP to prevent a fall in plasma ANP following TMeAVP never affected cardiovascular function. AVP infusion increased plasma volume accompanied by a fall in urinary Na excretion (UNaV) and urine flow (UF) under the stepwise plasma volume expansion compared to the control group, but did not affect mean circulatory filling pressure (MCFP). ANP administration enhanced UNaV and UF and decreased MCFP. These results indicate that AVP may preferentially increases ANP release via the increased cardiac afterload, not preload, but increased plasma ANP per se may not be involved directly in the AVP-induced cardiac suppression.View full abstractDownload PDF (1534K) -
SHINJI SATO, KIYOSHI ITO, NOBUYOSHI OZAWA, AKIRA YAJIMA, HIRONOBU SASA ...1991 Volume 165 Issue 2 Pages 131-136
Published: 1991
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSATO, S., ITO, K., OZAWA, N., YAJIMA, A. and SASANO, H. Analysis of Point Mutations at Codon 12 of K-ras in Human Endometrial Carcinoma and Cervical Adenocarcinoma by Dot Blot Hybridization and Polymerase Chain Reaction. Tohoku J. Exp. Med., 1991, 165 (2), 131-136-Samples of human endometrial carcinomas and cervical adenocarcinomas were screened for the presence of single site DNA mutations at codon 12 of the K-ras gene using dot blot hybridization of DNA amplified by the polymerise chain reaction (PCR). Of 21 cases of endometrial carcinoma, point mutations were observed in three cases (14.3%). Mutation from GGT to GTT was seen in one case, and mutation to GAT was observed in two cases. Of seven cases of cervical adenocarcinoma, point mutations were noted in two cases (28.6%). Mutation from GGT to GTT and double mutation to GAT and GCT were in one case each. However, no correlation was found between the presence of point mutation and age, clinical stage, or depth of muscular invasion. With respect to prognosis, of five patients with point mutation, one with cervical carcinoma died, and of 23 patients without mutation, one with endometrial carcinoma died.View full abstractDownload PDF (763K) -
SHINJI SATO, KIYOSHI ITO, NOBUYOSHI OZAWA, AKIRA YAJIMA, HIRONOBU SASA ...1991 Volume 165 Issue 2 Pages 137-145
Published: 1991
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSATO, S., ITO, K., OZAWA, N., YAJIMA, A. and SASANO, H. Expression of c-myc, Epidermal Growth Factor Receptor and c-erbB-2 in Human Endometrial Carcinoma and Cervical Adenocarcinoma. Tohoku J. Exp. Med., 1991, 165 (2), 137-145-Thirty specimens of human endometrial carcinoma (n=23) and cervical adenocarcinoma (n=7) have been analyzed for c-myc, epidermal growth factor receptor (EGFR) and c-erbB-2 by immunohistochemistry. In endometrial carcinomas, expression of c-myc was observed in all cases, EGFR in 21 of 23 cases (91.3%) and c-erbB-2 in 7 of 23 cases (30.4%). In cervical adenocarcinomas, expression of c-myc was seen in 5 of 7 cases (71.6%), EGFR in all cases and c-erbB-2 in 2 of 7 cases (28.6%). c-myc immunoactivity was observed as nuclear or cytoplasmic stain or both, EGFR as membrane and cytoplasmic stain, c-erbB-2 as menbrane stain. There was no relationship between expression of these three oncogenes and clinical prognostic factors in the present study.View full abstractDownload PDF (4438K) -
MEGUMI MATSUOKA, MASAYA SEGAWA, MAKOTO HIGURASHI1991 Volume 165 Issue 2 Pages 147-154
Published: 1991
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSMATSUOKA, M., SEGAWA, M. and HIGURASHI, M. The Development of Sleep and Wakefulness Cycle in Early Infancy and Its Relationship to Feeding Habit. Tohoku J. Exp. Med., 1991, 165 (2), 147-154-The purpose of this study was to evaluate the relationship between sleep and wakefulness patterns to feeding habits in early infancy. The population consisted of 33 neurologically normal infants studied during their first 4 months of life. The number of 30-min epochs with sleep (sleep epoch) were counted in each 4-hr period in a day and evaluated over time. The effects of feeding on sleep and wakefulness were examined by analyzing the rates of sleep epoch after feeding in each time period. The rates of sleep epochs in each time period showed specific patterns each week. From 2 weeks of age, sleep epochs appeared most frequently in time periods 0: 00-4: 00 and 4: 00-8: 00 (p<0.01). These periods also had significantly high rates of sleep epochs after feeding by week 2. From week 6 both the number of sleep epochs and the rate of sleep epochs after feeding in time periods from 8: 00 to 20: 00 tended to decrease. These results suggest that the development of the circadian oscillation is set as a sleep epoch first during the time period of 0: 00 to 8: 00. In addition, feeding alone seemed to have no role as a time cue in the first 4 months of life.View full abstractDownload PDF (867K) -
KOUHEI FUKUSHIMA, HIRONOBU SASANO, HIROSHI NAGURA, IWAO SASAKI, SEIKI ...1991 Volume 165 Issue 2 Pages 155-163
Published: 1991
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSFUKUSHIMA, K., SASANO, H., NAGURA, H., SASAKI, I., MATSUNO, S, and KROZOWSKI, Z.S. Immunohistochemical Localization of Mineralocorticoid Receptor in Human Gut. Tohoku J. Exp. Med., 1991, 165 (2), 155-163-Immunohistochemical localization of mineralocorticoid receptor in the human adult gut was examined by using a polyclonal antibody raised against a mineralocorticoid receptor fusion protein. Immunoreactive staining was observed in columnar cells in the middle of the intestinal glands of the proximal colon, but not in goblet cells. Western blot analysis also revealed the presence of an approximately 100kDa immunoreactive species consistent with the mineralocorticoid receptor protein in the ascending colon as well as the kidney. These results suggest that aldosterone-sensitive sodium transport and water absorption may be mainly carried out in the proximal colon in human adult gut at non-pathological state.View full abstractDownload PDF (3940K)
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