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Volume 182 (1997)
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Volume 182, Issue 1
May
Displaying 1-10 of 10 articles from this issue
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Regular Contributions
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Izabella Pawlik, Urszula Mackiewicz, Jan K. Lacki, Krzysztof Wiktorowi ...1997 Volume 182 Issue 1 Pages 1-8
Published: 1997
Released on J-STAGE: March 30, 2006
JOURNAL FREE ACCESSIn order to determine the role of memory/naive T cells in atopic allergy patients we analyzed peripheral blood mononuclear cells before and during the grass pollen season. The study comprised 28 patients with seasonal symptoms of atopic allergy and 18 with perennial symptoms. Flow cytometry was employed to detect the expression of CD3, CD4, CD4CD45RA, CD4CD45RO, CD8, CD16, and CD19 molecules on peripheral blood lymphocytes. Allergic patients showed a decreased proportion of memory (CD4+CD45RO+) T cells compared with healthy subject (p<0.05). The proportion of naive (CD4+CD45RA+) helper T cells did not differ between allergic patients and controls. The percentage of CD4+CD45RO+ cells increased during natural antigen exposure (grass pollen season) in allergic patients with seasonal symptoms. The results show at least two important observations. A potential homing tendency to nasal, bronchial and conjunctival mucosa of memory T cells (CD45RO) in atopic allergy patients may explain their deficiency in peripheral blood. Secondly, the grass pollen season may switch their phenotype from naive into memory T cells causing the increase of CD45RO cells. These events do not occur in non-allergic individuals and may thus constitute new insight into the basic mechanism of atopic allergy.View full abstractDownload PDF (517K) -
Keisaku Hatanaka, Nobukazu Nakasato, Kaoru Seki, Akitake Kanno, Kazuo ...1997 Volume 182 Issue 1 Pages 9-14
Published: 1997
Released on J-STAGE: March 30, 2006
JOURNAL FREE ACCESSMagnetic fields evoked by checkerboard pattern reversal visual stimulation to the monocular left or right half-field were recorded over the whole head using a helmet-shaped 64 channel magnetoencephalography system in fourteen normal subjects. The sources of the triphasic N75m-P100m-N145m responses were located using a single current dipole model. Relative locations and orientations of the N75m and N145m dipoles to the P100m dipole were calculated for each subject to reduce errors due to anatomical variability and then averaged for all subjects under the same stimulus conditions. These averaged parameters showed that N75m and N145m originated from the same location in the striate cortex as P100m and reversed their orientations successively by 180° during the time course.View full abstractDownload PDF (707K) -
Kunihiko Goto, Masatoshi Iwamoto1997 Volume 182 Issue 1 Pages 15-33
Published: 1997
Released on J-STAGE: March 30, 2006
JOURNAL FREE ACCESSThe three-dimensional structure of bacteriorhodopsin indicates that the all-trans-cis retinal bending causes α-helix slidings during the bacteriorhodopsin photocycle. For the elucidation of α-helix slidings taking place during the bacteriorhodopsin photocycle, we calculated ASAs of hydrophobic and hydrophilic atoms translocated by α-helix slidings with thermodynamic criteria found previously. Thermodynamic parameters calculated from ASAs (calculated ΔGtransfer and TΔS) were consistent with those (observed ΔGtransfer and TΔS) obtained empirically. These findings indicate that α-helix slidings take place during bacteriorhodopsin photocycle-energetics coupling. These mechanisms not only explain various phenomena, including some mutants forming a long-lived intermediate, but also predict various yet-unobserved phenomena, including the generation of heat in early photocycle intermediates.View full abstractDownload PDF (1130K) -
Hideyuki Kakizawa, Tadayuki Ohno, Akira Ozawa, Takashi Tanaka, Kazuie ...1997 Volume 182 Issue 1 Pages 35-39
Published: 1997
Released on J-STAGE: March 30, 2006
JOURNAL FREE ACCESSTwo infants with tetralogy of Fallot with absent pulmonary valve were studied with electrocardiographically gated magnetic resonance imaging using a 1.5 T imaging system. In each case, imaging was performed successfully. The magnetic resonance imaging clearly demonstrated a rudimentary pulmonary valve, an aneurysmal pulmonary artery, and compression of the mainstem bronchi. Cine MRI revealed phasic compression of the bronchi by an aneurysmally dilated pulmonary artery. Magnetic resonance imaging produced high quality images and provided all of the anatomic information required for preoperative evaluation.View full abstractDownload PDF (1274K) -
Zuo-Wen Zhang, Jiang-Bin Qu, Chan-Seok Moon, Shinichiro Shimbo, Takao ...1997 Volume 182 Issue 1 Pages 41-59
Published: 1997
Released on J-STAGE: March 30, 2006
JOURNAL FREE ACCESSIn 1993-1995, a nutritional survey was conducted in China, in which 50 women each in three cities of Beijing, Shanghai and Nanning (thus 150 in total) volunteered to offer 24-hr total food duplicates and peripheral blood samples. People daily took 1,776 kcal (7,431 kJ) energy, 57 g protein, 75 g lipid, 218 g carbohydrate, 439 mg calcium, 24 mg iron, 3,398 mg sodium and 1,521 mg potassium. When compared with the recommended dietary allowance for Chinese, intakes of energy, iron and possibly vitamin C were sufficient in a majority of the participants, but not so in cases of calcium, and several vitamins. The lipid energy ratio was 38% on an average and was higher than 30% in a majority (73%) of the people. BMI, but not the lipid energy ratio, correlated with the serum triglyceride level. In accordance with high iron intake, the prevalence of anemia was low. Inter-city differences were evident in nutrient sources. Thus, Beijing people took more amounts of protein and lipid from meats (including eggs and milk), whereas plant-based foods were important sources of lipid in Shanghai and that of protein in Nanning. Furthermore, consumption of rice was significantly lower in Beijing than in Shanghai and Nanning, whereas wheat consumption was higher in Beijing than in Shanghai and Nanning.View full abstractDownload PDF (1130K) -
Akio Tomoda, Eiichi Murakami, Takeshi Shibuya1997 Volume 182 Issue 1 Pages 61-67
Published: 1997
Released on J-STAGE: March 30, 2006
JOURNAL FREE ACCESSWe found that nitric oxide (NO) was produced during the oxidation of oxymyoglobin by nitrite, by using a NO-selective electrode system, which enabled us to measure NO in phosphate solution, continuously and quantitatively. When 500 μM nitrite was added in the solution of oxymyoglobin (65 μM in heme), oxymyoglobin was oxidized to metmyoglobin in a sigmoidal manner. During the reaction, NO was generated quickly at the initial lag phase, and reached its peak (NO approximated to 30 μM), before the burst oxidation of oxymyoglobin occurred. Then, NO content was gradually decreased. By the addition of the increased concentration of nitrite (1 mM or 2 mM) to oxymyoglobin solution (65 μM in heme), the production rate of NO was much accelerated and the amounts of NO were increased, in good accordance with the accelerated oxidation of oxymyoglobin by nitrite. These results suggest that NO is involved in the oxidation of oxymyoglobin by nitrite.View full abstractDownload PDF (377K) -
Yasuhiro Teranishi, Juro Iriuchijima1997 Volume 182 Issue 1 Pages 69-73
Published: 1997
Released on J-STAGE: March 30, 2006
JOURNAL FREE ACCESSWe have designated the hindquarter (or terminal aortic) vascular resistance (HQR) of the rat as hindquarter compensator (HC) because it increases after such hypotensive interventions as pentobarbital anesthesia and nitrate administration, presumably due to reflex excitation of regional vasoconstrictor fibers. The aim of the present study was to observe whether the HC mechanism is also mobilized in response to hemorrhage. Rats were implanted with a 1.5 or 2 mm diameter electromagnetic flow probe at the terminal aorta for measurement of hindquarter flow (HQF). An indwelling catheter was placed in the right common carotid artery to measure arterial pressure (AP) and withdraw blood. Experiments were performed in conscious rats two or three days after implantation. HQR was calculated by dividing AP by HQF. About 10 min after withdrawing blood (0.3 ml/100 g body weight), ganglionic blockade with hexamethonium bromide significantly decreased HQR, which indicated a mobilization of the HC mechanism, a change not observed with superior mesenteric resistance. A quantitatively similar change was observed in HQR after withdrawing double the amount of blood, i.e., 0.6 ml/100 g body weight, suggesting that the HC mechanism is activated almost fully by the relatively small amount of blood loss of 0.3 ml/100 g body weight.View full abstractDownload PDF (287K) -
Jun Tohyama, Hiroyuki Tsunoda, Norio Sakuragawa1997 Volume 182 Issue 1 Pages 75-82
Published: 1997
Released on J-STAGE: March 30, 2006
JOURNAL FREE ACCESSThis paper describes characteristics of human amniotic epithelial cells (AEC) transfected with a gene of origin-defective simian virus (SV) 40 large T-antigen (pMTIOD). Normal AEC before transfection with pMTIOD exhibited only low proliferative potential under our culture conditions. On the other hand, AEC cells transfected with pMTIOD exhibited greater proliferative potentials. Flowcytometry and immunohistochemistry analyses showed that both the primary and the transfected AEC did not express appreciable levels of class II antigens. However, the expression of class I antigen of the transfected AEC cells was slightly increased. The cells obtained in this experiment have the ability to induce tumors in severely combined immunodeficiency mice. This finding suggests that established AEC line can be used as a tool to investigate possible expression of the desired gene in human AEC and the gene products, however, was not suitable as a gene carrier to the recipient. Further experiments will be required to establish AEC as a transgene carrier for somatic cell gene therapy.View full abstractDownload PDF (959K) -
Shigeo Yamaki, Masato Endo, Tohru Takahashi1997 Volume 182 Issue 1 Pages 83-91
Published: 1997
Released on J-STAGE: March 30, 2006
JOURNAL FREE ACCESSMorphometric analysis of small pulmonary arterial changes was performed in three patients with different congenital heart disease with pulmonary hypertension: congenital mitral stenosis (MS), ventricular septal defect (VSD) and transposition of the great arteries (TGA). The material was biopsy or autopsy lung specimens, all having the same degree of elevated pulmonary arterial pressure. Medial thickness was determined by the method of Suwa and Takahashi, and the degree of intimal changes was quantified using the index of pulmonary vascular disease (IPVD) by Yamaki and Tezuka. It was demonstrated that the medial thickening of the small pulmonary arteries was the strongest in the patient with congenital MS, moderate in VSD, and the weakest in TGA, with statistically significant differences between each pair of these three conditions. Interestingly, the order of severity for intimal changes was reversed: it was the severest in TGA and the mildest in congenital MS. We surmise that in patients with TGA, medial hypertrophy is suppressed by sustained vasodilation resulting from the high oxygen saturation of pulmonary arterial blood, while in congenital MS, the media undergoes the severest hypertrophy because of the low oxygen saturation. We also conclude that in TGA intimal changes readily develop in the presence of attenuated media, while in congenital MS, the thickened media seems to prevent intimal changes. From a clinical viewpoint, these results urge us to recommend early surgical intervention in TGA and VSD where severe intimal changes can develop in the absence of extreme medial thickening. It may not be appropriate to extend operation on patients with congenital MS, since strongly thickened media can trigger vasospasms and medial necrosis.View full abstractDownload PDF (3164K)
Case Report
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A Case of Nonpenetrating Traumatic Aortic Regurgitation Detected by Transesophageal EchocardiographyHirotaka Oda, Toshiharu Tanaka, Yoshihiko Yamazaki, Eiichi Ito, Tsutom ...1997 Volume 182 Issue 1 Pages 93-101
Published: 1997
Released on J-STAGE: March 30, 2006
JOURNAL FREE ACCESSA 67-year-old man, who had fell 5 meters, landing on his back, one month before, was referred because of heart failure due to aortic regurgitation (AR). Transesophageal echocardiogram (TEE) confirmed injuries in the aortic valve and the Valsalva sinus of the aorta before the surgery: the intimal flap in the Valsalva sinus of right coronary cusp (RCC), the prolapse of the RCC, and the dissection by longitudinal length of 3 cm in the Valsalva sinus of noncoronary cusp (NCC), ending as a blind pouch. Postoperative TEE confirmed the dissection was not repaired in the Valsalva sinus of the NCC. In this instance, TEE was extremely useful, compared with transthoratic echocardiography, computed tomography and magnetic resonance imaging, to assess the mechanism of AR following a nonpenetrating trauma, and to know to what degree the aortic valve and the Valsalva sinus of the aorta were destroyed.View full abstractDownload PDF (4415K)
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