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Volume 189 (1999)
- Issue 4 Pages 233-
- Issue 3 Pages 171-
- Issue 2 Pages 87-
- Issue 1 Pages 1-
Volume 189, Issue 2
October
Displaying 1-8 of 8 articles from this issue
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Regular Contributions
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Koji Tanoguchi, Nobuo Yaegashi, Kumiko Jiko, Akihiko Maekawa, Shinji S ...1999 Volume 189 Issue 2 Pages 87-93
Published: 1999
Released on J-STAGE: July 05, 2005
JOURNAL FREE ACCESSThe Donryu rat has been found to have a high incidence of spontaneous uterine endometrial carcinomas. Moreover the histologic findings, biological nature and pathogenesis of these rat tumors appear similar to those in humans. To determine if the incidence of H- and K-ras gene mutations in these rat tumors is similar to that in human endometrial cancers, we isolated DNA samples from 2 atypical hyperplasias, 5 simple or complex hyperplasia without atypia, 9 adenocarcinomas and 7 histologically normal tissues, amplified exons 1 and 2 of the H- and K-ras genes by PCR and hybridized the products with allele specific oligonucleotide probes. K-ras point mutations were observed in 1/2 of the atypical hyperplasia (codon 12: GGT→GTT) and 3/9 of the carcinoma (codon 12: GGT→GAT, GGT→AGT, codon 61: CAA→CAC), while they were not detected in 7 of the normal tissues and in 5 of the simple or complex hyperplasia without atypia. H-ras point mutations were not detected in any of these DNA samples. These frequencies in this rat model are similar to those in humans. The absence of K-ras mutations from simple and complex hyperplasia tissue samples suggests that these mutations are associated with cytological atypia. Our findings suggest that alterations in the K-ras gene may be one of the important initiating event in endometrial carcinogenesis in some of the Donryu rat, like the human.View full abstractDownload PDF (780K) -
Makoto Satoh, Fatemeh Moosavi Nejad, Osamu Nakano, Akihiro Ito, Sadafu ...1999 Volume 189 Issue 2 Pages 95-105
Published: 1999
Released on J-STAGE: July 05, 2005
JOURNAL FREE ACCESSClinicopathological studies revealed that monosialosyl galactosyl globoside (MSGG) and disialosyl galactosyl globoside (DSGG) expressed by renal cell carcinoma (RCC) are one of the biochemical indicator related to the metastatic potential. The present study examines the characteristics of four new human RCC cell lines and compares the expression of MSGG and DSGG among them using TLC immunostaining and flow cytometry. TOS-1 and TOS-2 were derived from metastatic subcutaneous tissues. TOS-3 and TOS-3LN were derived from the primary lesion and from metastatic lymph nodes respectively. Monolayer culture, light microscopy and electron microscopy of these cells showed that these cell lines were derived from RCC. TLC immunostaining and flow cytometric analysis revealed increased levels of MSGG in TOS-2 and TOS-3LN, and increased DSGG in TOS-1 and TOS-3LN. These cell lines would be useful for functional studies of globo-series ganglioside expressed by RCC.View full abstractDownload PDF (1559K) -
Ayse Balci, Abdullah Ekmekci, Recep Çetin1999 Volume 189 Issue 2 Pages 107-116
Published: 1999
Released on J-STAGE: July 05, 2005
JOURNAL FREE ACCESSThe frequency and distribution of aphidicolin induced common fragile sites was evaluated on chromosomes of peripheral blood lymphocytes in 10 breast and 10 colorectal cancer patients, and 10 healthy controls to determine correlation between specific fragile sites and cancer breakpoints. Fifty complete metaphases were screened from each culture and the results were evaluated by Student's t-test. The total number of fragile sites was found as 933 in breast cancer patients, 950 in colorectal cancer patients and 501 in control group. Both the number of aberrations per cell and number of aberrations per damaged cell were significantly higher in the patient groups. These findings indicate that genetic instability in the breast and colorectal cancer patients increased and fragile sites may play a critical role in the pathogenesis of breast and colorectal cancer.View full abstractDownload PDF (567K) -
Takuma Narita, Jun Koshimura, Katsunori Suzuki, Masahiko Murata, Hiroy ...1999 Volume 189 Issue 2 Pages 117-133
Published: 1999
Released on J-STAGE: July 05, 2005
JOURNAL FREE ACCESSTo determine whether each of glycemic control (GC), low protein diet (LPD) or administration of angiotensin converting enzyme inhibitor (ACEI) has beneficial effects on diabetic nephropathy through the different mechanisms, changes in charge and size selectivity of glomerulus and renal hemodynamics were analyzed in microalbuminuric type 2 diabetic patients after additive combination therapy (first period: GC only, second period: GC+LPD, third period: GC+LPD+ACEI). To detect improvement of the impairments of glomerular charge selectivity and size selectivity, changes in the ratio of the renal clearance of two plasma proteins with similar molecular radii and different isoelectric points (pIs) (ceruloplasmin and IgG: CRL/IgG) and changes in the ratio of the renal clearance of two plasma proteins with similar pIs and different molecular radii (α2-macroglobulin and albumin: α2/Alb) were examined before and after each therapy. Creatinine clearance decreased significantly in the first and third periods although slight but not significant decrease was detected in the second period. Filtration fraction was significantly decreased only in the third period. Although renal clearances of Alb, IgG and CRL were decreased in periods of all three therapies, that of α2-macroglobulin with a large molecular radius was decreased significantly only after the third therapy. Neither CRL/IgG nor α2/Alb changed during these three therapies. These findings suggest that each of three short-term therapies consisting of GC, GC+LPD and GC+LPD+ACEI, reduced proteinuria in microalbuminuric type 2 diabetic patients not through the improvement of renal size and charge selectivities, but through improvement of renal hemodynamics.View full abstractDownload PDF (1086K) -
Takashi Kondo, Akira Horikoshi, Shigefumi Fujimura, Yuji Matsumura, Yo ...1999 Volume 189 Issue 2 Pages 135-145
Published: 1999
Released on J-STAGE: July 05, 2005
JOURNAL FREE ACCESSEffect of α-adrenergic blockers on pulmonary edema in lung transplantation was studied with a rat model of syngeneic left lung transplantation. Prior to harvesting, 0.1 mg of Prazocin or 0.4 mg of Yohimbin was given to the donor. Pulmonary and systemic hemodynamics were measured under the right pulmonary arterial occlusion (RPAO) at different time points after grafting. Wet to dry weight ratio (W/D) of all transplants was also calculated. Same procedure was conducted in rats with normal and ischemic lung and in transplanted animals without any treatments. While RPAO did not increase W/D in normal lung with a significant elevation in pulmonary arterial pressure (PAP), both these values significantly increased in transplanted lung. Transplanted animals could not tolerate RPAO 24 hours after grafting, but were tolerable later than 48 hours with elevated W/D and PAP. On the contrary, animals given Prazocin or Yohimbin were all tolerable at 24 hours postsurgery. Yohimbin significantly improved W/D. Consequently, it was demonstrated that pulmonary edema of the graft reached its peak during first 24 to 48 hours after transplantation and was alleviated by the blockade of α-adrenergic recepter in the graft vessel.View full abstractDownload PDF (642K) -
Shigeru Ehara, Shigehiko Katsuragawa1999 Volume 189 Issue 2 Pages 147-153
Published: 1999
Released on J-STAGE: July 05, 2005
JOURNAL FREE ACCESSThe purpose of this investigation is to assess the importance of clinical information for the detection of non-displaced wrist fractures in children. Twenty non-displaced fractures of the distal radius in children younger than 15 years of age and twenty age-matched controls were evaluated by five blinded observers before and after giving clinical data, and a receiver operating characteristic (ROC) analysis using a continuous rating scale with a line-marking method was performed. The detection of the fractures was significantly improved with clinical information, and the main reason for this was an increase in true positive fraction. Availability of adequate clinical data should be emphasized for interpreting radiography.View full abstractDownload PDF (1156K)
Case Reports
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Hiroshi Tanaka, Shinobu Waga, Takashi Tateyama, Kazuhiko Sugimoto, Yos ...1999 Volume 189 Issue 2 Pages 155-161
Published: 1999
Released on J-STAGE: July 05, 2005
JOURNAL FREE ACCESSA boy aged 4 years with nephrotic syndrome (NS) was referred to our hospital because of the third relapse of NS. Hypogammaglobulinemia associated with massive proteinuria was observed at the presentation. Residual urinary tract infection required intravenous piperacillin and immunoglobulin therapy (IVIG). Soon after IVIG, he complained of high fever with chills, bilateral knee joint pain, dry cough and chest discomfort. Although he did not develop renal insufficiency, a transient increase in the urinary β2-microglobulin and decrease in the serum complement hemolytic activity were observed. These clinical manifestations spontaneously ceased. A percutaneous renal biopsy for his NS performed 19 days after the episode of allergic rection revealed tubulointerstitial nephritis (TIN) with marked eosinophil infiltrates. Glomeruli showed minor glomerular abnormalities. Renal complications associated with IVIG treatment have been reported to date, however, acute TIN has rarely been seen.View full abstractDownload PDF (1034K) -
Yuji Kasukawa, Kyoji Okada, Manabu Hashimoto, Masato Sageshima1999 Volume 189 Issue 2 Pages 163-170
Published: 1999
Released on J-STAGE: July 05, 2005
JOURNAL FREE ACCESSExtra-abdominal desmoid tumor of the hand is rare and only 10 cases have been described in the literature. We present a 14-year-old boy with a recurrent extra-abdominal desmoid tumor in the dorsal site of the right hand. MR image demonstrated the tumor in the third dorsal interosseous muscle, and adhered to the radial side of the forth metacarpal bone. The lesion revealed iso-signal intensity on T1-weighted images and high intensity on T2. We performed a marginal excision. Histological examination of the tumor showed proliferation of the fibroblastic cells with abundant collagen bundles. He developed local recurrence for the third time. The size of the third recurrent tumor has not been changed for 2 years and 3 months. Therefore, we have not performed any additional surgery. Since extensive resection markedly diminishes the function of the hand, we consider that a marginal surgical margin is acceptable for the quality of daily life of patients with a desmoid tumor of the hand.View full abstractDownload PDF (2084K)
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