The effect of simultaneous administration of p-dimethylaminoazobenzene (D. A. B.) and carbon tetrachloride (CCl
4) was experimentally studied on rats.
1) The morphological changes in the early stage of simultaneous administration of D. A. B. and CCl
4 were characterized by an earlier appearance of cholangiofibrosis or of liver cirrhosis than in those produced by D. A. B. or CCl
4 alone.
2) Simultaneous administration of both agents invariably resulted in statisti-cally significant higher incidences of liver carcinoma, liver cirrhosis, liver carcinoma with liver cirrhosis, and nodular hvperplasia than the administration of D. A. B. alone.
3) There was no histological difference between the liver carcinomas caused by combined use of D. A. B. and CCl
4, and those by the administration of D. A. B. alone. 4) CCl
4 caused a decrease in protein-bound azo-dye in the liver in the course of carcinogenesis by D. A. B.
5) The mechanism of the activity of CCl
4 in the development of D. A. B. -induced liver carcinoma was discussed on the basis of the relation between the incidence of liver carcinoma and the amount of protein-bound azo-dye in the liver.
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