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Volume 152 (1987)
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- Issue 2 Pages 103-
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Volume 152, Issue 1
Displaying 1-12 of 12 articles from this issue
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FEDERICO FRANCIONI, TETSURO NISHIHIRA, MASAYUKI MASUDA, MICHIHIKO KITA ...1987 Volume 152 Issue 1 Pages 1-14
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSFRANCIONI, F., NISHIHIRA, T., MASUDA, M., KITAMURA, M., AKAISHI, T., SHINEHA, R., SEKINE, Y. and MORI, S. The Significance of Pre-Operative Diagnosis of Esophageal Cancer Using Esophageal Mediastinal Ultrasonography. Tohoku J. exp. Med., 1987, 152 (1), 1-14 - Esophageal ultrasonography (EUS) is a new diagnostic method which allows improved preoperative diagnosis of esophageal cancer. Careful examination of EUS findings and comparison of these findings with operative and pathological findings yielded criteria useful for the determination of tumor depth and lymphnode metastasis. Concerning tumor depth. EUS findings of 30 patients were reviewed and compared with operative and pathological findings. Based on this comparison some diagnostic criteria were then chosen; it was found that the accuracy was around 87% and that the differentiation between intramural and extraluminar invasion could be easily made using these criteria. Regarding the lymphnodes, at the time of operation, the site and size of each lymphnode (a total of 267 nodes) were recorded. These findings were grouped according to the site and size. We found the range of sizes of metastasic lymphnodes to be great and not so different from that of normal nodes. We then compared these anatomical findings with the EUS findings in an attempt to determine appropriate diagnostic criteria, which would include size as well as intrinsic and extrinsic echocharacteristics. When these criteria were used, the accuracy was about 90%. Although this preliminary study must be confirmed by further prospective studies with a large number of patients, we can conclude that EUS allows accurate determination of tumor depth and node metastasis in patients with esophageal cancer.View full abstractDownload PDF (2630K) -
KAZUHIKO OYANAGI, KEIJI TSUBAKIHARA, YOSHIHIRO ITAKURA, MASAYOSHI NAGA ...1987 Volume 152 Issue 1 Pages 15-21
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOYANAGI, K., TSUBAKIHARA, K., ITAKURA, Y., NAGAO, M., TAKAYANAGI, N., TSUCHIYAMA, A., NAKAO, T., INOUE, I. and SAHEKI, T. Immunoenzymatic Studies of Ornithine Transcarbamylase (OTC) in Liver of Patients with OTC Deficiency by Enzyme Linked Immunosorbent Assay (ELISA). Tohoku J. exp. Med., 1987, 152 (1), 15-21 - Antigen contents of ornithine transcarbamylase (OTC) protein in the liver of 5 patients with OTC deficiency and mutant mice were studied by ELISA. OTC activities in the liver tissues of 2 patients were 13.1% and 5.2% of the controls, respectively. The 2 patients' antigen contents of OTC protein were decreased in parallel with the enzyme activities. OTC antigen contents were not detected in the liver tissues of remaining 3 patients who had the complete type of OTC deficiency. Enzyme activities, kinetic properties and antigen contents of the OTC deficient mutant mice were the same as the results reported previously (Briand et al. 1982). The ELISA method for the assay of OTC antigen contents used in this study is more sensitive as compared with the radial immunodiffusion technique.View full abstractDownload PDF (529K) -
MASAYOSHI NAGAO, KAZUHIKO OYANAGI, AKIRA TSUCHIYAMA, TAKUO AOYAMA, TOO ...1987 Volume 152 Issue 1 Pages 23-29
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNAGAO, M., OYANAGI, K., TSUCHIYAMA, A., AOYAMA, T. and NAKAO, T. Studies on the Expression of Liver-Specific Functions of Human Fetal Hepatocytes in Primary Culture. Tohoku J. exp. Med., 1987, 152 (1), 23-29 - Biochemical functions of human livers were studied using fetal hepatocytes in primary culture. Immunocytochemical staining showed that albumin was not expresed in any fetal hepatocytes, whereas α-fetoprotein was detected in almost all the cells. Tryptophan 2, 3-dioxygenase (TO, EC 1.13.11.11.) activity was not induced in the presence of 10-7M dexamethasone and 10-7M glucagon, but the activity of tyrosine aminotransferase (TAT, EC 2.6.1.5.) was elevated about 35 fold under the same conditions. These results suggest that the TAT and α-fetoprotein genes are activated in human fetal liver at 14 to 20 weeks of gestation.View full abstractDownload PDF (1615K) -
KAZUHIKO OYANAGI, MASAYOSHI NAKAO, AKIRA TSUCHIYAMA, TOORU NAKAO1987 Volume 152 Issue 1 Pages 31-34
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOYANAGI, K., NAGAO, M., TSUCHIYAMA, A. and NAKAO, T. Control of Growth and Amino Acid Transport of Mature Rat Hepatocytes by Human Newborn Serum. Tohoku J. exp. Med., 1987, 152 (1), 31-34 - DNA synthesis and system A-mediated amino acid transport were studied in primary culture of adult rat hepatocytes maintained as a monolayer. Potent growth promoting activity and inducibility of system A-mediated amino acid transport could be found in human newborn sera but not in human adult sera. The maximal activity to promote DNA synthesis was about the same level as insulin (10-7M) plus epidermal growth factor (EGF, 20ng/ml). These results suggest the presence of a hepatocyte-specific growth factor in human newborn sera controlling the amino acid metabolism during the newborn period.View full abstractDownload PDF (382K) -
YOSHIO SUZUKI, JUNKO UCHIDA, HIROSHI TSUJI, KEIHACHIRO KUZUHARA, SHIGE ...1987 Volume 152 Issue 1 Pages 35-45
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSUZUKI, Y., UCHIDA, J., TSUJI, H., KUZUHARA, K., HARA, S., NIHEI, H., OGURA, Y., OTSUBO, O. and MIMURA, N. Acute Changes in C3a and C5a in an Anaphylactoid Reaction in Hemodialysis Patients. Tohoku J. exp. Med., 1987, 152 (1), 35-45 - Blood sample were collected from both the arterial and venous lines of the patients undergoing hemodialysis using cuprophan membrane at several time intervals. Radioimmunoassay was then applied to measure the C3a and C5a levels. C3a levels from the arterial lines of patients undergoing uneventful hemodialysis were 463.2±51.3ng/ml (mean±S.D.), 1112.9±109.9, 956.0±72.1, and 721.2±49.6 at 0, 15, 120 and 360min, respectively. C5a levels did not change significantly during uneventful hemodialysis. C3a and C5a levels were significantly higher in the blood from the venous lines than those from the arterial lines, a finding indicating that complement activation via alternative pathway takes place in the dialyzer. C3a and C5a levels were markedly elevated in patients with anaphylactoid reaction. Reuses of dialyzers on the patients having a history of an anaphylactoid reaction during hemodialysis suppressed not only the rise of C3a levels, but also the recurrence of the reaction. From these findings, it is concluded that rises of C3a and C5a in the arterial lines of patients are causally related to the induction of anaphylactoid reaction during hemodialysisView full abstractDownload PDF (904K) -
KAZUHIKO OYANAGI, YOSHINORI KUNIYA, MASAYOSHI NAGAO, AKIRA TSUCHIYAMA, ...1987 Volume 152 Issue 1 Pages 47-51
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOYANAGI, K., KUNIYA, Y., NAGAO, M., TSUCHIYAMA, A. and NAKAO, T. Cytotoxicities of Sodium Benzoate in Primary Culture of Hepatocytes from Adult Rat Liver. Tohoku J. exp. Med., 1987, 152 (1), 47-51 - The cytotoxicities of sodium benzoate was studied using primary culture of hepatocytes established from adult rat liver by a collagenase perfusion technique and maintained as a monolayer in serum-free culture medium. The activities of ornithine transcarbamylase (as a marker of mitochondria) and tyrosine aminotransferase (as a marker of cytosol) were clearly suppressed by sodium benzoate at concentration in excess of 500μg/ml. Intracellular protein synthesis and DNA synthesis were also suppressed, and the suppression of DNA synthesis was observed even with a lower concentration of benzoate (100μg/ml).View full abstractDownload PDF (378K) -
TADASHI ARIGA, MOTOHIKO OKANO, YUTAKA TAKAHASHI, YUKIO SAKIYAMA, SHUZO ...1987 Volume 152 Issue 1 Pages 53-61
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSARIGA, T., OKANO, M., TAKAHASHI, Y., SAKIYAMA, Y. and MATSUMOTO, S. Analysis of B Cell Dysfunction in Patients with Common Variable Immunodeficiency by Using Recombinant Interleukin 2. Tohoku J. exp. Med., 1987, 152 (1), 53-61 - Recombinant interleukin 2 (RIL2) induces proliferation and differentiation of the Staphylococcus aureus Cowan I (SAC)-activated normal B cells to immunoglobulin (Ig) producting cells. We applied this finding to an analysis of heterogeneity in the differentiation states of B cells in patients with common variable immunodeficiency (CVI). B cells from 5 of 7 patients with CVI were tested for their ability to proliferate under the stimulation of SAC, or SAC and RIL2, and for their differentiation to Ig producing cells in the presence of SAC plus RIL2. The results suggest that the differentiation status of B cells in CVI could be divided into four states based on their responses to SAC and RIL2. In the first state, no B cell proliferation or differentiation was demonstrated in our assays. B cells in the second state showed normal proliferative responses to SAC, but not to SAC plus RIL2, and no differentiation to Ig-secreting cells. The third state showed normal proliferation in responce to SAC and SAC plus RIL2, but no differentiation to Ig-secreting cells. The fourth state had a normal proliferation response to SAC and SAC plus RIL2, and normal secretions of IgG and IgM in response to SAC and RIL2. These results show that some B cells in CVI have defects in response to RIL2, and at least in terms of defect of B cells, CVI is heterogeneous.View full abstractDownload PDF (857K) -
YASUO TAKAHASHI, TETSURO OGAWA1987 Volume 152 Issue 1 Pages 63-65
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSTAKAHASHI, Y. and OGAWA, T. Effects of Lowered pH of the Extracellular Solution on Non-Transsynaptic Component of Evoked Potentials in the Rat's in Vitro Cerebral Cortical Slices. Tohoku J. exp. Med., 1987, 152 (1), 63-65 - The effects of lowered extracellular pH on non-transsynaptic component of field potentials evoked by electric stimulation of the white matter which were recorded in layer IV of the occipital cerebral cortex were studied with the rat's in vitro brain slices. At a range of pH between 6.2 and 7.2, the amplitude of the non-transsynaptic potentials was decreased with a complete recovery to the initial normal level when the pH was restored to 7.4. With a decrease in amplitude, the latency of evoked potentials was also delayed a little in the acidic media.View full abstractDownload PDF (230K) -
HARUYUKI OSHIMA, SHO ISHIKAWA, REIKO IRIE, TAKEHIKO TACHIBANA1987 Volume 152 Issue 1 Pages 67-80
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOSHIMA, H., ISHIKAWA, S., IRIE, R. and TACHIBANA, T. Expression of Alien H-2 and Non-H-2 Antigens on a Murine Mammary Tumor Cell Line, MM46. Tohoku J. exp. Med., 1987, 152 (1), 67-80 - Tumor-associated surface antigens on MM46, a transplantable ascites tumor from a spontaneous mammary carcinoma in a C3H/He mouse, were investigated. A complement-ependent microcytotoxicity test showed the expression of MM antigen, whose specificity appeared to be identical or cross-reactive with that of Ly-6.2 antigen, as reported previously. Moreover, MM46 was also found to express alien H-2 antigenic specificities, H-2.31 and H-2.28. H-2.31 represents the private specificity of the H-2Kd molecule, while H-2.28 represents a public specificity among molecules encoded by H-2K, H-2D, and H-2L loci in the majority of original H-2 haplotypes, including H-2d, but not H-2k, of inbred laboratory mice. The expression of MM antigen was found to be closely associated with the appearance of hypotetraploid tumor cells. These results suggest that alien H-2 and non-H-2 antigens appeared during ascites tumor cell conversion.View full abstractDownload PDF (1192K) -
MASAMITSU UNAKAMI, ITARU KOMIYA, YUZO ENDO, MITSURU HARA, KENJI IKEDA, ...1987 Volume 152 Issue 1 Pages 81-86
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSUNAKAMI, M., KOMIYA, I., ENDO, Y., HARA, M., IKEDA, K., KUMADA, H., YOSHIBA, A., HINO, O. and KITAGAWA, T. Seroconversion from HBs-Ag to Anti- HBs in a Case of Liver Cirrhosis Associated with Hepatocellular Carcinoma. Tohoku J. exp. Med., 1987, 152 (1), 81-86 - This paper reports a case of liver cirrhosis associated with hepatocellular carcinoma (HCC) of a woman who was converted from hepatitis B surface antigen (HBs-Ag) positive to antibody against HBs-Ag (anti-HBs) positive in the serum through an immunoregulatory steroid rebound phenomenon. The histology of the biopsy specimen taken before the seroconversion showed an early stage of liver cirrhosis with moderate infiltration of mononuclear cells. At autopsy about 3 years after the seroconversion, the liver tissue free of the tumor was in an early stage of liver cirrhosis. Fibrosis did not advance as compared with the biopsy specimen. In addition, mononuclear cell infiltration decreased remarkably and piecemeal necrosis disappeared after the seroconversion. The immunohistologic examination of hepatocytes demonstrated that positive stainings for HBs-Ag and for hepatitis B core antigen (HBc-Ag) in the biopsy specimen turned to be negative in the autopsy specimen. These facts indicate that the steroid rebound phenomenon eliminated free hepatitis B virus (HBV) in the hepatocytes in the absence of massive necrosis of hepatocytes. HBV-DNA integration was proved in the genome of HCC by molecular hybridization method.View full abstractDownload PDF (971K) -
MINGCHANG HE, SENMING HUN, LIN ZHANG, CHENHUA LIAN1987 Volume 152 Issue 1 Pages 87-100
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSHE, M., HUN, S., ZHANG, L. and LIAN, C. In Vitro Alterations of Tracheal Epithelium of Hamsters by Carcinogens in Organ Culture. Tohoku J. exp. Med., 1987, 152 (1), 87-100 - In vitro organ culture of the hamster's trachea was improved and applied to a carcinogenesis research. The rotary culture enabled explants of tracheal epithelium to survive more than 8 weeks. The study was composed of 2 kinds of culture; untreated and treated with carcinogens. In the untreated culture, Eagle MEM medium had the same culture effect as RPMI 1640 medium. With prolongation of culture time (particulary longer than 5 weeks), irreversible degenerative changes appeared in epithelial cells. Culture for 4 weeks was usually thought to be appropriate for experimental research. In the treated culure, the effect of benzo-(a) pyrene (B(a)P) and B(a)P+cigarette smoking condensate-neutral fraction (CSC-NF) on tracheal epithelium was investigated with light and electron microscopies (TEM and SEM) and autoradiography. Atypical hyperplasia with or without lesions suggesting carcinoma in situ was induced by B(a)P+CSC-NF more evidently and frequently than by B(a)P alone. The present findings corroborated the cocarcinogenetic effect of CSC-NF.View full abstractDownload PDF (3715K) -
YURIKO SHINDOH, YASUO TANNO, SHIRO IDA, TAMOTSU TAKISHIMA1987 Volume 152 Issue 1 Pages 101-102
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSHINDOH, Y., TANNO, Y., IDA, S. and TAKISHIMA, T. Morphological Characterization of Basophilic Cells in Bronchoalveolar Lavage Fluids from Patients with Bronchial Asthma and Idiopathic Pulmonary Fibrosis. Tohoku J. exp. Med., 1987, 152 (1), 101-102 - A marked increase in the number of basophilic cells (BCs) was found in the bronchoalveolar lavage (BAL) fluids from the patients with bronchial asthma and idiopathic pulmonary fibrosis (IPF). Histochemical analysis revealed that basophils were the major components of BCs in asthmatic patients, while formalin-insensitive BCs, which are presumed to be connective tissue mast cells, were observed in BAL fluids from IPF patients. In control subjects, almost all of BCs were mucosal mast cells.View full abstractDownload PDF (1860K)
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