日本薬理学雑誌
Online ISSN : 1347-8397
Print ISSN : 0015-5691
ISSN-L : 0015-5691
50 巻 , 4 号
選択された号の論文の17件中1~17を表示しています
  • 松本 順三
    1954 年 50 巻 4 号 p. 337-357,en25
    発行日: 1954/07/20
    公開日: 2011/09/07
    ジャーナル フリー
    Functional and cytochemical experiments were carried out on the cardiac movement and dephosphorylation in the hearts of frogs, toads and mice with a combination of digitalis glycoside such as digitoxine or g-strophanthine, ATP and DNase following Shionoya's 3rd method and Allen's method, from which the following results were obtained : DNase and ATP, generally, cause a stimulant action on the isolated frog's heart. Moreover, ATP has a potentiation of its action to DNase. On the other hand, DNase shows a synergistic action to the therapeutic phase of digitoxine or g-strophanthine. Furthermore, DNase and ATP have a specific effect on digitalis glycoside. (1) DNase shows a powerful antagonistic action to the toxic phase of digitoxine. (2) ATP shows the same action which, however, is somewhat weaker. (3) A combination of digitalis glycoside and DNase shows a powerful accelerated dephosphorylation in the heart tissues of the toads and mice. (4) Individual digitalis glycoside or a combination of digitalis glycoside and ATP shows the same dephosphorylation which, however, is somewhat weaker. The above results suggest that there exist synergistic effects on the cardiotonic actions among the therapeutic phase of digitalis glycoside, ATP and DNase ; they especially suggest that the combined effects on the cardiotonic actions among them increase in proportion to acceleration of dephosphorylation in the heart. They can be considered to provide further support for the previous ly proposed Nakatsuka's hypothesis that digitalis glycoside accelerates, primarily, nucleic acid metabolism and causes, secondarily, an increase in the rate of breakdown of energy-rich phosphate in the myocardium ; namely, that digitalis glycoside accelerates -ATP metabolism in the heart muscle through direct interaction with nucleic acid metabolism.
  • 松原 孝善
    1954 年 50 巻 4 号 p. 358-360,en25
    発行日: 1954/07/20
    公開日: 2011/09/07
    ジャーナル フリー
    On the basis of the previous reports of the other authors on the capillary-like patterns observed during the cultivations in vitro of fowl's leucocytes a reexamination was schemed. Result : Under the little modified conditions of cultivations it was true that the capillary-like patterns were formed within 24 hours from the beginning of cultivations, but thereafter the elongation of the patterns stopped and there remained only the pile of erythrocytes and leucocytes in the capillary tube, where the endothelial structures were no longer observed. The micro-scopic findings showed that these capillary-like patterns are of a degenerative sign of leucocytes, so that if only the cultivating conditions, which are mainly related to their physical properties in this case, were suitable enough, these patterns would be ever proliferating.
  • 荻生 規矩夫, 島本 暉朗, 松原 孝善
    1954 年 50 巻 4 号 p. 361-366,en26
    発行日: 1954/07/20
    公開日: 2011/09/07
    ジャーナル フリー
    In succession to the first report on the pharmacological studies on the in vitro cultivated ergot, the fluid extract of the mycelium and the filtrate of some ergot species (Claviceps purpurea separated by Prof. Katagiri, Claviceps purpurea separated from the rye-ergot, which were imported from North America and Claviceps separated from ergot of Agrogyrum semicostatum), which were cultivated in vitro on various synthetic culture media modifing the cultural pH, cultural period and cultural temperature, were studied systematically. The results obtained are as follows : 1) The oxytocic potency of the fluid extract of the mycelium of the third were 4 to 8 times stronger than that of the first and 2 to 4 times stronger than the second on the extirpated virgin guinea pig's uterus in vitro. Only the extract of mycelium of the latter showed the cock comb reaction and the adrenolytic action on the extirpated uterus and ileum of rabbit in vitro. As the amounts of histamine in these extracts, assayed by Dale-Schulz's method using the extirpated guinea pig's ileum, were considerably small, the oxytocic substances in the extracts resulted from the specific ergot alkaloids. 2) On the other hand, the fluid extracts of all the filtrates showed the cock comb reaction and adrenolytic action as well as the oxytocic action similar to that of the mycelium. 3) The alternation of the cultural conditions using various composition of natural and artificial cultural media and pH did not modified the pharmacological action essentially. However the oxytocic potency of the fluid extract of mycelium on artificial cultural media were somewhat stronger than that on natural cultural media. 4) Alternation of the cultural period for 1 to 4 weeks and the cultural temperature for 5 to 27°C also did not modified the pharmacological action of the fluid extract of mycelium and filtrate.
  • 松原 孝善
    1954 年 50 巻 4 号 p. 367-370,en26
    発行日: 1954/07/20
    公開日: 2011/09/07
    ジャーナル フリー
    A pharmacological experiment was carried out on the fluid-extract of in vitro-culture of Claviceps parasited on “Tsuruyoshi, ” Phragmites prostratus Makino, grown wildly in the 'Kyoto district and gathered in December 1949, and it was proved that the fluid-extract exerted a strong contraction of the isolated uterus in rabbits and guinea-pigs, which may be attributed to ergotamine, probably being existed in this fluid-extract.
  • 内田 亨
    1954 年 50 巻 4 号 p. 371-383,en26
    発行日: 1954/07/20
    公開日: 2011/09/07
    ジャーナル フリー
    The present paper reports results of studies on influence of antihistaminics over the effects of epinephrine and stimulation of the sympathetic and vagus on functions of various organs as well as the activity of aminoxidase. 1) Benzhydryldimethylaminoethanolether (Benadryl) and N, N-thiodiphenyl-N ; N'-dimethylethylenediamine (Anergen) sensitize peripheral organs against epinephrine as well as the sympathetic stimulation, and they inhibit the aminoxidase activity. 2) N, -N-dimethyl-N'-phenyl-N' (2-thienylmethyl) -ethylenediamine (Diatrin) does not inhibit the aminoxidase, and its epinephrine and sympathetic sensitization is unpronounced and variable. 3) Effects of the three antihistaminics on functions of various organs were determined.
  • 岡本 悟一
    1954 年 50 巻 4 号 p. 384-392,en27
    発行日: 1954/07/20
    公開日: 2011/09/07
    ジャーナル フリー
    The potent anticonvulsant action with longer duration has been proved with 3- (1, 2, 3, 4-tetrahydro-8-naphthyloxy) -1, 2-propanediol (α-TNP) and 3-(1, 2, 3, 4-tetrahydro7-naphthyloxy) -1, 2-propanediol (β-TNP) by Omiya (see this Folia, vol. 49, no. 4, pp. 159, 1953). The author has proved also that they have the analgesic, anti-histaminic, anti-cholinergic and quinidine-like actions.
  • 島本 暉朗, 藤田 貞雄, 安原 基弘, 小川 保直
    1954 年 50 巻 4 号 p. 393-401,en27
    発行日: 1954/07/20
    公開日: 2011/09/07
    ジャーナル フリー
    Using the drop-timer, designed lately by the authors, the blood vessels of the extirpated frog's leg and of the rabbit's ear were perfused by the usual laboratory technic with Ringer-Locke's solution. The mode of action of adrenaline and acetylcholine were studied by discussing the effects of some other autonomic. drugs upon the action of adrenaline and acetylcholine. Results : 1) At the beginning of the perfusion of the frog's leg-vessels, the rhythmic increase and decrease of outflow of perfusate were observed. 2) At the beginning of the perfusion of both preparations, it was often seen that the injection of adrenaline (10-7-10-9) increased the outflow, but after the preparations were stored in 0°C for 12 hrs, the increase of outflow could be seen no more; but only a decrease of outflow. 3) Acetylcholine decreased the outflow of the frog's leg-vessels almost without exception, but increased the outflow of the rabbit's ear-vessels occasionally, which responded to a decrease of outflow of the rabbit's ear-vessels occasionally, and responded to a decrease of outflow without exception after it had been_ stored in 0°C for 12 hrs. 4) Parasympathicomimetics, such as betaine methyl- and ethylesters and pilocarpine inhibited the decreasing action of adrenaline in dilute concentration, but potentiated in high concentration. Pilocarpine also inhibited the decreasing action of acetylcholine. 5) The decreasing actions of adrenaline and acetylcholine were potentiated by eserine and DFP in dilute concentration, but they were inhibited or prevented by large doses of DFP. 6) The decreasing action of adrenaline and acetylcholine were potentiated by the amall doses of atropine, but were inhibited or prevented by large doses. 7) Adrenolytics, such as priscol, privine, Dibenamine and reygostine inhibited or prevented the decreasing action of adrenaline and acetylcholine. 8) Nicotine potentiated the decreasing action of adrenaline and acetylcholine by small doses, and inhibited or prevented by large doses. From the pharmacological results above mentioned the modes of constrictor action of nicotine and acetylcholine (nicotine-like action) were qualitative similar to that of adrenaline. In discussing the details of the results, the authors concluded that nicotine and acetylcholine had the same site as adrenaline, and that the constrictor action of nicotine and acetylcholine resulted from the liberation of sympathin-like substances at the effector cell.
  • 藤原 榮一
    1954 年 50 巻 4 号 p. 402-409,en28
    発行日: 1954/07/20
    公開日: 2011/09/07
    ジャーナル フリー
    The results of experiments with rabbits are as following : (1) Three per cent alcohol was injected in doses of 100 cc/ kg intraperitoneally to rabbits two weeks or more in succession until they became habituated to alcohol and the variation of the concentration of alcohol in blood was pursuited. In the habituated rabbits the *concentration of alcohol in blood became more rapidly high and decreased more quickly than that in the non-habituated rabbits. Its maximum concentration was higher in the habituated rabbits than that in the non-habituated ones. (2) The injection of alcohol subsequent to the administration of antabuse resulted in the very high concentration of alcohol in blood and the delay of alcohol metabolism in both the habituated and non-habituated rabbits. The concentration of alcohol in blood in the habituated rabbits, as compared with the one in the non-habituated ones lowered more quickly after it had once become high. (3) By the injection of alcohol only, the concentration of acetaldehyde in blood in the habituated rabbits, as compared to the one in the non-habituated ones, was more higher and fell off more quickly after it had gotten high. (4) When alcohol was injected-subsequently to the administration of antabuse into both the nonhabituated and the habituated rabbits, in the former the variation of the concentration, of acetaldehyde in blood scarcely differed with the one when only alcohol was given, but in the latter the concentration of acetaldehyde in blood increased remarkably, and acetaldehyde metabolism was delayed. (5) In the brain wave of the non-habituated rabbits the injection of alcohol induced disappearance of the fast wave and appearance of the prominent slow wave, this indicating the inhibitory effect of alcohol. The fast wave was apt to increase by the injection of alcohol as habituation of rabbits to alcohol proceeded. The brain wave of the nonhabituated rabbits which was obtained by the injection of alcohol subsequent to the administration of antabuse hardly differed with the one by the injection of alcohol only. In the brain wave of the habituated rabbits, on the contrary, the injection of alcohol after the administration of antabuse resulted in appearance of the fast wave in succession with intermittent spikes, to say the convulsive wave properly, this showing the significant effect of antabuse.
  • 小柳津 隆
    1954 年 50 巻 4 号 p. 410-421,en29
    発行日: 1954/07/20
    公開日: 2011/09/07
    ジャーナル フリー
    The protective action of salicylates against anaphylactic responses (shock as well as Schulz-Dale reaction) on guinea pigs, which were sensitized actively with egg-albumin or passively with serum of rabbit treated with egg-albumin. Result : (1) In the case of the active sensitization, anaphylactic responses were inhibited and animals survived against challenge when a sufficient large dose of sodium salicylate or acetylsalicylic acid was given repeatedly during all stages or at the middle stage of sensitizing treatment. (2) Passive anaphylaxis was similarly interferred with sodium salicylate when a sufficient dose of the drug was applied at the proper stage, i.e. within 12 hours from the beginning of the sensitizing procedure. (3) In guinea pigs sensitized with rabbit anti-egg-albumin serum, which were treated preliminary with salicylates during a period more than 24 hoursin vitro, the anaphylactic death was not observed. (4) It could be supposed that the salicylates had no effect on the circulating antibody-level.
  • 赤堀 和一郎
    1954 年 50 巻 4 号 p. 422-432,en29
    発行日: 1954/07/20
    公開日: 2011/09/07
    ジャーナル フリー
    Methemoglobin formation after the aromatic amino- or nitro-compounds was proved by the spectroscopic investigations following with the lapse of time of observations and its mechanism was explained on the basis of electrotonic theory with reference to the literatures of other authors ever published. On his opinion Fe··in hemoglobin will be oxidized to Fe··· directly or indirectly due to the electrotonic potency of available drugs tested and thus methemoglobin will be formed by neutralization of the increased charges of Fe ion with OH- in the solution.
  • 田窪 重徳, 森岡 英二
    1954 年 50 巻 4 号 p. 433-442,en29
    発行日: 1954/07/20
    公開日: 2011/09/07
    ジャーナル フリー
    The relation between the blood volume in the stomach wall and the gastric secretion is yet almost.unknown. In the present experiment, the blood volume in the gastric wall was traced by means of our apparatus, detail of which was already published in the 'previous paper, to observe the relation between the blood volume in the stomach wall and the gastric secretion, using the dog with a gastric fistula. After giving pilocarpine, acetylcholine, histamine, or electrical stimulus, both the blood volume and the gastric secretion increased. When given B.I. the increase of gastric secretion was not always accompanied with the increase of the blood volume.
  • 村松 正美
    1954 年 50 巻 4 号 p. 443-456,en29
    発行日: 1954/07/20
    公開日: 2011/09/07
    ジャーナル フリー
    The following results were obtained concerning the brain wave of cats by the administration of several kinds of anesthetic. 1) By ether, chloroform, and morphine hydrochloride, the α wave decreased, the β wave appeared clearly, and the amplitude of the brain wave became small, resulting in the flat wave on the whole. In the case of chloroform, in addition, the slow wave with large amplitude came to appearance. 2) Phenobarbital brought about the slowing of the brain wave, together with the induced large amplitude. 3) The slow wave with large amplitude appeared and the α wave disappeared immediately after the injection of amytal and seconal. 4) Magnesium sulfate made the remarkable β wave appear and the amplitude small. 5) By paraldehyde the amplitude of the brain wave became small and the α wave disappeared, resulting in the flat wave. The effects upon the brain wave differed in phenobarbital, seconal, and amytal, and ether, chloroform, morphine, paraldehyde, and magnesium ions. Difference in the site of action of each drug should be responsible for this fact.
  • 鏑木 暸應
    1954 年 50 巻 4 号 p. 457-463,en30
    発行日: 1954/07/20
    公開日: 2011/09/07
    ジャーナル フリー
    The hydrolysate of the mucous membrane of hog stomach treated by the diluted sulphuric acid has the most remarkable effect on the experimental ulcer of the rabbit and the toad in comparison with the hydrolysate of the muscular part of the stomach or intestine. The healing of the ulcer has been accelerated by the continuous administration of the hydrolysate consequently such treated toads survived longer than the control animals. The hydrolysate restrained the gastric juice secretion. Likewise the total acidity and content of free hydrochloric acid in the juice decreased. Furthermore, the hydrolysate showed the haematopoietic action. The increase of erythrocytes and reticulocytes was observed in the rabbits which were given the hydrolysate injections. The chemical structure of the active principle in the hydrolyse is still unknown but through the paperpartition chromatography the active principle seems to be a peptide like substance which is attendant on some amino acid.
  • 鏑木 瞭應
    1954 年 50 巻 4 号 p. 464-477
    発行日: 1954/07/20
    公開日: 2011/09/07
    ジャーナル フリー
    The hydrolysate of the mucous membrane of hog stomach treated by the diluted sulphuric acid has the most remarkable effect on the experimental ulcer of the rabbit and the toad in comparison with the hydrolysate of the muscular part of the stomach or intestine. The healing of the ulcer has been accelerated by the continuous administration of the hydrolysate consequently such treated toads survived longer than the control animals. The hydrolysate restrained the gastric juice secretion. Likewise the total acidity and content of free hydrochloric acid in the juice decreased. Furthermore, the hydrolysate showed the haematopoietic action. The increase of erythrocytes and reticulocytes was observed in the rabbits which were given the hydrolysate injections. The chemical structure of the active principle in the hydrolyse is still unknown but through the paperpartition chromatography the active principle seems to be a peptide like substance which is attendant on some amino acid.
  • 仲井 睦彦
    1954 年 50 巻 4 号 p. 478-480,en30
    発行日: 1954/07/20
    公開日: 2011/09/07
    ジャーナル フリー
    In his previous paper the author reached the conclusion, that intralumbarly applied percaine (dibucaine) is rapidly absorbed in almost half the amount of the dose into the circulation; and the circulatory as well as the respiratory depression induced by the lumbar injection of the drug is caused halfly by that part of percaine which escaped toward the blood. The present experiment is to test the rapid absorption of drugs out of the lumbar canal. Methylene blue was injected into the lumbar canal of rabbits. Venous vessels nearby, Vena cava inferior, etc., lungs and heart were found blue colored by the dye within five minutes. Adrenalin applied in the same way increased, if slowly, the blood pressure (Fig. 1). Acetylcholine decreased it when given intralumbarly as rapidly as given intravenously (Fig. 2). The drugs must have been absorbed into the blood and fixed at the peripheral blood vessels, where they are held to display the specific action. In cases of hypodermic injection a thirty or fifty times larger dose of adrenalin or acetylcholine was necessary to elicit the pressor action of the same degree as in cases of lumbar injection. The absorption of adrenalin and acetylcholine from the lumbar canal is achieved incomparably more easily than from the subcutaneous tissue.
  • 北郷 武照, 上野 昭
    1954 年 50 巻 4 号 p. 481-484,en31
    発行日: 1954/07/20
    公開日: 2011/09/07
    ジャーナル フリー
    It has been found by Hongo that Ringer's solution, kept with rabbit plasma for 18 hours at 38°C, decreased the blood pressure in cats. The present study aimed to follow the mode of action of this substance, being employed this plasma as a solid component of the tissue culture medium in the Carrel's bottle. Result : The hypotensive effect for cat was not affected by the preliminary intravenous injection of atropine and benadryl, while it was antagonized by adrenaline. Such a hypotensive action was not observed in rabbit and it was also proved that this substance had no effect on the uterus of albino rat.
  • 松家 利男
    1954 年 50 巻 4 号 p. 485-493,en31
    発行日: 1954/07/20
    公開日: 2011/09/07
    ジャーナル フリー
    INAH in large toxic doses produces a slight fall in blood pressure which is followed by a sudden rise of blood pressure accompanied invariably by convulsions and a contraction of nictitating membrane in cats. The aforesaid rise of pressure and contraction of nictitating membrane are neither inhibited by atropine nor by d-tubocurarine, but by benzylimidazoline and TEAB. The convulsions, however, are depressed by d-tubocurarine, but not by benzylimidazoline or TEAB. Convulsions and the rise of blood pressure are 136th depressed to a certain extent by deep narcosis and completely by decapitation. The results lead to the following conclusions : INAH in large doses causes central nervous system stimulation, resulting in convulsions. The rise, of blood pressure results from stimulation of sympathetic vasoconstrictor centre.
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