The auther studies on the intrahepatic hemodynamics in experimentally induced acute portal obstruction in dogs. The Silica suspension was injected into the intrahepatic portal vein and the changes of portal venous pressure, wedged hepatic venous pressure, hepatic artery blood flow, portal blood flow and portographic findings were observed. Furthermore the influences of the hepatic artery ligation and application of various drugs such as Adrenalin, Pitressin, Buscopan, Pyrethia, Pacatal, or Procain which affect the portal circulation were also investigated. The results are summerized as follow: 1) The wedged hepatic venous pressure elevated in pallalel with elevation of portal venous pressure. In the group the hepatic artery being ligated the pressure gradient gradually increased with elevation of the portal venous pressure, whereas in the groups the drugs being administrated these pressures elevated pallalel. 2) The portal blood flow was markedly decreased converse to elevation of the portal venous pressure, while the hepatic artery blood flow was gradually increased. The flow rate of portal vein and hepatic artery was in the ratio 4.79: 1 before injection of silica, but after injection upon the degree of 350mm H2O of portal venous pressure, there were in ratio 2: 1. 3) In the portographic observation there were tendency that the portal vein was opened within the sphere of hepatic vein catheter obstruction, while on the other part the portal vein was obstructed. Accordingly it is suggested that portal venous and wedged hepatic venous pressure elevation in the acute intrahepatic portal obstruction have been resulted from the portal venous pressure elevation due to mechanical obstruction of the portal bed, and the reflection of the elevated portal venous pressure to the hepatic vein through the opening portal branches and furthermore the elevation of the sinusoidal pressure caused by compensatory augmentation of the hepatic artery blood flow against the decrease of portal blood flow.
Cell kinetics of the mucosal cells in various parts of the gastrointestinal tract was investigated in normal mice with 12-15 g of body weight using autoradiography with H3-thymidine and the following results were obtained: 1) Mitosis was observed only in the following area: stomach (5×14μ to 12×14μ), duodenum (1 to 5×14μ), jejunum (1 to 7×14μ) and ileum (1 to 4×14μ)-all figures represent the distance from the muscularis mucosa. In mucosal cells of the mice sacrificed 1 hour after intraperitoneal injection with H3-thymidine, H3-labeled cells were found only the same area as above described. These results indicate that the cells in this area consist of the proliferative compartment. 2) From the analysis of the fraction of mitoses labeled in the mucosal cells of the mice sacrificed at various time following single injection with H3-thymidine, the following results were obtained: 3) In gastric mucosa, distribution of the labeled cells spread out both to the gastoric pit and to the muscularis mucosa and attained the level of the muscularis mucosa at 21 hours following single injection with H3-thymidine. In apex of the gastric pit, labeled cells are found in the autoradiogram obtained from the mice sacrificed 46 hours after the injection. In the intestinal mucosa, distribution of the labeled cells spread out only to the intestinal villi, and attained at the apex at 43 hour in duodenum, 43 hour in jejunum and 46 hour in ileum after the injection. Autoradiograms obtained from the mice sacrificed 73 hours after the injection, percentage of the labeled cells was low at any part of the mucosa, suggesting that most of the labeled cells were removed by this time. 4) Mean grain count of the labeled cells in the proliferative compartment was reduced exponentially during the time course from single intraperitoneal injection with H3-thymidine at either part of the intestinal or gastric mucosa. Mean generation time of the mucosal cells of proliferative compartment of the stomach, duodenum, jejunum and ileum calculated from these results were 35, 14, 14 and 14 hours, respectively. The analysis of the mean grain counts both in the proliferative and in the functional compartment revealed that the cells of the latter compartment mitosed at least once in the former compartment on an average.