Studies of glucose-
14C(U) and pyruvate-
14C (U) metabolism in liver tissues obtained by needle biopsy under peritoneascopy from patients with acute hepatitis (convalescence), chronic hepatitis (active and inactive form), cirrhosis of the liver (types A, A' and B, and the nutritional), fatty liver and diabetes mellitus and in liver slices from rats in acute and chronic carbontetrachloride intoxication revealed the following:
1) Metabolic rates as measured by the incorporation of labeled carbon from glucose-
14C(U) and pyruvate-
14C(U) into CO
2, glycogen, fatty acids and glucose with 10-15mg tissues of human liver were comparable to those obtained similarly with about 120mg slices of rat liver.
2) A statistically significant decrease in incorporation of labeled carbon from glucose-
14C(U) into glycogen and an increase from pyruvate-
14C(U) into fatty acids were found in convalescence from acute hepatitis. Incorporations of the radioisotope from glucose-
14C(U) into CO
2, glycogen and fatty acids tended to decline in chronic hepatitis (active form), diabetes mellitus, chronic hepatitis(active from) with diabetes mellitus and cirrhosis of the liver (types A and A').
Decreases in the total incoporation into CO
2, glycogen and fatty acids appeared to be correlated with retarded restoration of blood sugar level in a oral glucose tolerance test. Similarly, cases with impaired oxidation of pyruvate-
14C(U) by liver tissue showed a delay in restoration of blood pyruvate level towards the fasting value after the glucose load.
3) In acute carbontetrachloride intoxication of rats, increased procuctions of radioisotope CO
2 and glycogen from glucose-
14C (U) and decreased productions of the CO
2 and glucose from pyruvate-
14C (U) and an increased formation of
14C-glycogen from pyruvate-
14C (U) were found to be statistically significant to control values.
In chronic carbontetrachloride intoxication, productions of the CO
2 and glycogen from glucose-
14C(U) and oxidation of pyruvate-
14C(U) were all significantly decreased; the results being similar to those observed with clinical cases of chronic hepatitis and cirrhosis of the liver.
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