1) A total of 74 adult female rats (inbred Moriyama so-o) were served for a series of experiment, in which proximal 50%, proximal 75%, distal 50%, distal 75% and more than 90% of the small intestine distal to the ligament of Treitz was resected, and after complete rerecovery, gastric secretion and gastro-intestinal motility were examined by means of Shay's method as well as Nylander's technique. 2) It was proved with the method of Shay that postoperative gastric hyperacidity occurs on the rats, in which over 75% of small intestine was removed (referred to massive resection, hereafter), favoring the incidence of peptic ulcer after massive resection. This fact is consistent with the result from Heidenhain-pouched dogs which were reported by previous workers. 3) The Nylander's approach to the motility of gastro-intestinal tract, using 51Cr as an indicator, depicted that the gastric emptying was significantly depressed and the duodenal motility was slightly retarded after massive resection. 4) The above finding could be accounted for on the basis that the massive resection induces an excess of functional load on the remaining intestinal segemnt, which may result in a delay of emptying in the stomach through enterogastric reflex and then an acceleration of acid secretion. 5) In prevention of postoperative gastric disorder, it may be advisable that massive resection of small intestine should be limited with the reservation of gut segment of more than 1.5 times the body length.
A D-galactose-1-14C solution was injected into the superior mesenteric vein or the hepatic artery of the rats. Ten seconds after the injection, the hepatic artery and the portal vein were ligated, and the liver was resected. Per cent intrahepatic shunt was calculated from the radioactivity of the resected liver and that of the injected solution added to the liver with same kind of lesion. When the solution was injected into the mesenteric vein, per cent intrahepatic shunt of rats with fatty liver produced by feeding of choline deficient diet and rats with acute carbon tetrachloride intoxication was almost the same as the normal rats. Rats with hepatic fibrosis showed slight increase, and rats with hepatic cirrhosis marked increase in per cent intrahepatic shunt. There was no significant difference of per cent intrahepatic shunt between rats with hepatic carcinoma produced by feeding of 3'-methyl-DAB and normal rats. When the solution was injected into the hepatic artery, per cent intrahepatic shunt of rats with normal liver was higher than that of the mesenteric venous injection. The difference of per cent intrahepatic shunts in rats with hepatic cirrhosis between the mesenteric venous injection and the hepatic arterial injection was lesser than that in normal rats. Per cent intrahepatic shunt of rats with hepatic carcinoma increased markedly when the solution was injected into the hepatic artery. This can be explained by the facts that the cancerous tissue is supplied by the hepatic artery and the intrahepatic shunted blood flow in the cancerous tissue is not measured by the method of intra-portal injection but of intra-arterialinjection. Increase of per cent intrahepatic shunt in rats with hepatic carcinoma is ascribed to abnormal galactose metabolism in tumor tissue rather than to appearance of the hepatic arterio-venous anastomoses. Abnormal metabolism of galactose in hepatic carcinoma produced by feeding of 3'-methyl-DAB was shown in an experiment: 14CO2 production from D-galactose-1-14C in tumor slices decreased markedly as compared with that in the control liver.
The validity of measurement of activity and iso-enzymatic analysis of β-glucuronidase in basal gastric juice as one of tools for the diagnosis of gastric cancer has been evaluated. β-gl. activity was measured by the method described by Tsukamoto. Iso-enzymatic analysis was performed with starch block electrophoresis. Followings are the results. 1) 9 of 13 cases of early gastric cancer and 37 of 41 advanced gastric cancer showed activity higher than 1200 units of β-gl. 2) 2 cases of 47 benign gastric ulcer and 7 cases of 59 atrophic gastritis showed the activity higher than 1200 units. 3) Homogenate of gastric cancer tissue showed higher specific activity of β-gl. than that of the gastric mucosa of normal, ulcer and gastritis cases. 4) Starch block electrophoresis of homogenate of gastric cancer tissue or cancerous mucosa for β-gl. showed four peaks; 2 small peaks on the anodal side, one small peak on the origin, and one big peak on the cathodal side. These peaks are tentatively called peak I, II, III, IV, respectively. Homogenate of normal mucosa shows only peak III, IV, and no difference of electrophoretic patterns were found among antrum, lesser curvature and greater curvature of the body of the stomach. In cases of atrophic gastritis or ulcer, either peak I, III, IV, or peak III, IV, were noted. 5) Gastric juice of normal controls, atrophic gastritis or peptic ulcer showed either peak I, IV or peak IV. On the other hand, gastric juice of gastric cancer showed characteristic patterns; the presence of peak II and/or III was almost specific and was found in 18 out of 19 cancer cases, including 4 cases of early cancer. Only 2 of 30 controls showed peak I, III, IV pattern. These were severe cases of diffuse gastritis erosiva. 6) In conclusion, electrophoretic analysis as well as measurement of activity of β-gl. could be one of supplemental tools for the diagnosis of gastric cancer.