A 46 year-old man was admitted Shinko Hospital because of jaundice. He had a history of heavy alcoholic intake for many years and a very large intake just before his admission. The diagnosis of acute alcoholic hepatitis (pure cholestatic type) was made. Features of laboratory and morphologic findings were discussed. The electrophoretic pattern of serum γ-GTP isoenzymes-complex was analyzed and it was considered to be a very useful diagnostic aid in such a case. By the light microscopy, centrolobular cholestasis in addition to wire-mesh fibrosis and slight fatty infiltration was observed in the biopsy specimen from the liver. Although Popper hypothesized that intrahepatic cholestasis is the result of hypoactive and hypertrophic smooth endoplasmic reticulum in the hepatocytes, its proliferation was not shown by the electron microscopic observation in our case.