The aldolase activities toward Fructose-l. 6-diphosphate (FDP-ALD) and Fructose-I phosphate (FIP-ALD) were assayed by the method of spectrophotometric measurement of reduced nicotinamide adenine dinucleotide (NADH) at 340mμ in the sera of the patients with various liver diseases and in the supernatant fluid from the homogenates of the various tissues of normal adult rats. Zone electrophoresis of aldolase isozyme in the sera of the patients described above and of the patients with malignant tumor was carried out on cellulose acetate strips.
The results were the following:
1. FDP-ALD activity was remarkable in the skeletal muscle, moderate in the liver and brain, and slight in the lung, kidney, and spleen of the normal rats. but FIP-ALD activity was remarkable in the liver and slight in the other tissues. FDP-ALD/FIP-ALD activity ratio (FDP/FIP) was remarkable in the skeletal muscle (45.92±2.88) and slight in the liver (1.19±0.007). In other tissues, it was moderate.
2. Normal human serum showed the FDP-ALD activity of 5.01±1.71m-I.U., FIP-ALD activity of 2.47±0.67m-I.U. and FDP/FIP of 2.01±0.21, indicating that the main origin of the serum aldolase would be the liver.
3. Aldolase activities toward both substrates were significantly increased but FDP/FIP was significantly decreased in each serum of the patients with acute hepatitis and active form of chronic hepatitis in comparison with those in the normal human serum. This fact suggested that the increase of this enzyme in these diseases was derived from damaged liver,
4. Slight increase of aldolase activities toward both substrates and nearly the same ratio of FDP/FIP were observed in each serum of the patients with non-active form of chronic hepatitis and liver cirrhosis in comparison with those in the normal human serum.
5. Electrophoretic patterns of aldolase isozyme in the sera of the patients with various liver diseases and with malignant tumor using both substrates showed unexpectedly only one band at the α
2-β globurin area of the normal human serum protein.
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