Rat's experiments were undertaken to examine the alterations of steps involved in the transfer of BSP from blood to bile after bilateral nephrectomy. 1) Following a single intravenous administration of BSP, biliary excretion of BSP and bile output soon after bilateral nephrectomy were increased. Biliary excretion of BSP and bile output at 24 hours following bilateral nephrectomy were increased in some rats and decreased in some ones. 2) Distrubution of BSP after a constant infusion of BSP was estimated. The maximal transport of BSP into bile (biliary BSP-Tm) soon after bilateral nephrectomy was decreased to some extent and the hepatic storage of BSP after one hour's constant infusion of BSP was distinctly increased. The increase of hepatic BSP storage was brought about with an increase of unconjugated BSP. Conjugation rate of the biliary BSP was unchanged and that of the plasma BSP was elevated. At 24 hours following bilateral nephrectomy, the biliary BSP-Tm was decreased more distinctly and the hepatic storage of BSP was variable. Conjugation rate of hepatic BSP was somewhat lower than the control and that of plasma BSP was the same as the control. 3) It was concluded that an increase of biliary excretion of BSP was brought about without any elevation of biliary BSP-Tm and conjugation of BSP. Thus, it suggests that an increase of hepatic cellular permeability may have a role to increase biliary excretion of BSP in bilaterally nephrectomized rats.
Rat's experiments were undertaken to examine the alterations of steps involved in the transfer of BSP from blood to bile following an intravenous administration of sodium salicylate. 1) Following a single intravenous administration of BSP, a moderate degree of choleresis and a significant increase of biliary excretion of BSP were observed. 2) Distribution of BSP was examined after a constant infusion of BSP. The maximal transport of BSP into bile (biliary BSP-Tm) was unchanged through a constant intravenous administration of sodium salicylate. A significant increase of hepatic BSP storage, in unconjugated form, was demonstrated. 3) Through an addition of sodium salicylate a significant inhibition of BSP-binding affinity of plasma albumin and a distinct release of BSP from BSP-bound serum were demonstrated in vitro. No acceleration of BSP-glutathione conjugating enzyme activity was demonstrated. 4) It was concluded that an increase of biliary excretion of BSP by sodium salicylate was brought about through an inhibition of BSP-plasma albumin binding.
Although many studies on the genesis of the peptic ulcer have been reported, the precise process developing to the ulcer is not yet clarified completely. In this study, the administration of"Cinchophen"to the dog was adopted for making the chronic ulcers, because of its validity of easy production of the ulcers which are macroscopically and microscopically similar to those of human. The single course of administration of"Cinchophen"was given 48 dogs and other 14 dogs were observed serially on the developmental process of the ulcers using per-peritoneal gastroscope and GTF. In the group of the single administration; 1) The ulcer could be recognized in 43 among 48 dogs (89.6%). 2) The single ulcer was found in 7 cases (16.3%) and the multiple ulcers were found in the other 36 cases (83.7%). 3) The ulcers located at the border section between the different glandular areas, ranged to the deep layers and were ranked as the grade of ul III or ul IV. 4) The form of the ulcers was almost round or oval. 5) Excepting at the above-mentioned border section, there were generally observed the shallow ulcers which were classified as ul II with their diameters less than 10mm in 80%. In the group administered the repeated courses of"cinchophen"under the serial abservation by gastroscopy; 1) There appeared multiformed hemorrhagic erosions and shallow ulcers in all cases, with the total dosage of 2-10gm (6.4gm on the average) of"Cinchophen"during 3-8 days (5.3 days on the average). 2) Most of them were healed during the successive administration of"Cinchophen". 3) Erosions located at the border zone were apt to develop to ulcers. 4) Speaking of 12 dogs in which gastric ulcers were observed, average days requiring to develop to ulcers were 1.1 days (6-18 days) and the mean dosage was 14.1gm (7.5-21.0gm). It was clarified, through this study, the multiple hemorrhagic erosions produced by"Cinchophen"were healed in most of cases, while those located at the border zone between the different glandular areas had a tendency to develop to ulcers reaching the deeper layers.
Spotty hepatic cell necrosis is said to be one of the characteristic histological findings of viral hepatitis. It is the purpose of this investigation to elucidate the submicroscopical change of reticulin framework associated with the necrosis. Three cases of acute viral hepatitis were selected for the study. Of the three cases two were icteric cases of serum hepatitis at the 34th and 19th day after the appearance of jaundice, and another one was a non-icteric case of infectious hepatitis. The results of the study were briefly summarized in the following; 1) The change of reticulin framework associated with hepatic cell necrosis was observed as early as icteric stage of acute viral hepatitis. 2) In accordance with the mode of hepatic cell necrosis, hydropic swelling on the one hand and condensation on the other, a difference was observed in the proliferation of connective tissue fiber associated with the change of reticulin framework. 3) In the area where the necrotic cells showed a degenerative change of coagulation, the proliferation of collagen fiber was intense. A substance of high electron density was observed in the Disse, s space, and a microfibrils of 450Åin diameter was laid down in a condensed fashion. The proliferation of fiber rather than the approximation of the pre-existing reticulin fiber was incriminated to the fibrogenesis of such areas. The areas where the necrotic cells showed a degenerative change of hydropic swelling, on the other hand, the rupture of the cell membrane was conspicuous. The scattering of the cell organella was found. The proliferation of connective tissue fiber had not yet begun. A scanty fibrous structure was found inside of the endothelial cell lining. 4) The matrix cells related to the hepatic fibroplasia were searched with special reference to the Kupffer cells, endothelial lining cells, macrophages and so on. No close relation was as yet observed between the production of fibrills and such cells.