With a view to find a mechanism of a characteristic lowering of polarographic filtrate wave (Pfw) of 20-minute aspirate after insulin injection when compared to that of fasting gastric juice, which is called by the name of “Polarographic cancer pattern” of gastric juice, patients were divided into 2 groups, i. e., the cancerous group and healthy acidic controls, and fasting 20-minute and 40-minute aspirates drawn from respective groups were fractionated into four fractions (from F
5 to F
8) by the author's modification of Glass & Boud's method.
On each of the fractions thus obtained, polarographic behaviour was investigated.
The Pfws in both control and cancerous specimens were the highest in F
5 (acetone-soluble fraction), and showed perpendicular figures similar to that of native gastric juice.
In addition, in the cancerous specimen, a lowing of Pfw in F
5 was obtained in the 20-minute aspirate.
From these facts, it may be declared that F
5 is a fraction having the characteristic of “Polarographic cancer pattern”.
In the control specimens, typical polarographic double waves were noticed in F
8 (mucoprotein fraction according to Glass), but little polarographic activities were observed both in F
6 and in F
7 (mucoproteose fraction according to Glass).
On the contrary in the cancerous specimens, Pfws were noticed in F
6 and F
7, while they were negligible in F
8.
In summary of Part I and Part II, it is concluded that the several interfering factors, e. g., contaminations by saliva, blood, and bile have no influence on the “Polarographic cancer pattern” per se.
In the cancerous specimen, it was observed that polarographic activity existed in F
7 but not in F
8, which was a reverse result to that obtained with the control specimen.
Among the fractionated samples, F
5 seems to play an important rôle in the above cancer pattern.
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